pDC415

价格:1800元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Microbix Biosystems
载体名称: pDC415
质粒类型: 腺病毒表达载体
高拷贝/低拷贝: --
启动子: MCMV
克隆方法: --
载体大小: 5951 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素(Ampicillin)
筛选标记: --
备注: 克隆宿主菌:DB3.1
产品目录号: --
稳定性: --
组成型: 组成型
病毒/非病毒: 腺病毒

载体质粒图谱和多克隆位点信息

pDC415载体图谱



载体简介

载体序列

LOCUS       pDC415       5951 bp dsDNA    Circular
DEFINITION  Puc based MCMV shuttle plasmid  for Ad vector rescue via homologous recomb. Ng et al. Hum. Gene Ther. 11: xxx, 2000.
COMMENT     This file is best viewed with a free Vector NTI Viewer:
COMMENT     http://www.informaxinc.com/view/viewer.html
COMMENT     VNTREPLTYPE|Plasmid|
COMMENT     VNTEXTCHREPL|Bacteria|
FEATURES               Location/Qualifiers
     promoter        469..991
                     /note="MCMV promoter - Short"
                     /label=MCMV
     misc_feature    1029..1167
                     /label=SV40
     misc_feature    1167..3343
                     /label=Ad
     rep_origin      3641..3941
                     /label=ori
     misc_feature    5492..453
                     /label=Ad
     CDS             complement(4391..5207)
                     /label=amp
     CDS             5861..1
                     /label=ITR
     CDS             complement(2..102)
                     /label=ITR
ORIGIN
        1 ttcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt 
       61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg 
      121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg 
      181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt 
      241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa 
      301 gtgaaatctg aataattttg tgttactcat agcgcgtaat atttgtctag ggccgcgggg 
      361 actttgaccg tttacgtgga gactcgccca ggtgtttttc tcaggtgttt tccgcgttcc 
      421 gggtcaaagt tggcgtttta ttattatagt cagntctaga gatatactga gtcattaggg 
      481 actttccaat gggttttgcc cagtacataa ggtcaatagg ggtgaatcaa caggaaagtc 
      541 ccattggagc caagtacact gagtcaatag ggactttcca ttgggttttg cccagtacaa 
      601 aaggtcaata gggggtgagt caatgggttt ttcccattat tggcacgtac ataaggtcaa 
      661 taggggtgag tcattgggtt tttccagcca tttaattaaa acgccatgta ctttcccacc 
      721 attgacgtca atgggctatt gaaactaatg caacgtgacc tttaaacggt actttcccat 
      781 agctgattaa tgggaaagta ccgttctcga gccaatacac gtcaatggga agtgaaaggg 
      841 cagccaaaac gtaacaccgc cccggttttc ccctggaaat tccatattgg cactcattct 
      901 attggctgag ctgcgttcta cgtgggtata agaggcgcga ccagcgtcgg taccgtcgca 
      961 gtcttcggtc tgaccaccgt agaacgcaga tcgaattcaa gctgctagca aggatccagc 
     1021 ttgtcgactt cgagcaactt gtttattgca gcttataatg gttacaaata aagcaatagc 
     1081 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 
     1141 ctcatcaatg tatcttatca tgtctggatc gtctagcatc gaagatcgat ctgggcgtgg 
     1201 ttaagggtgg gaaagaatat ataaggtggg ggtcttatgt agttttgtat ctgttttgca 
     1261 gcagccgccg ccgccatgag caccaactcg tttgatggaa gcattgtgag ctcatatttg 
     1321 acaacgcgca tgcccccatg ggccggggtg cgtcagaatg tgatgggctc cagcattgat 
     1381 ggtcgccccg tcctgcccgc aaactctact accttgacct acgagaccgt gtctggaacg 
     1441 ccgttggaga ctgcagcctc cgccgccgct tcagccgctg cagccaccgc ccgcgggatt 
     1501 gtgactgact ttgctttcct gagcccgctt gcaagcagtg cagcttcccg ttcatccgcc 
     1561 cgcgatgaca agttgacggc tcttttggca caattggatt ctttgacccg ggaacttaat 
     1621 gtcgtttctc agcagctgtt ggatctgcgc cagcaggttt ctgccctgaa ggcttcctcc 
     1681 cctcccaatg cggtttaaaa cataaataaa aaaccagact ctgtttggat ttggatcaag 
     1741 caagtgtctt gctgtcttta tttaggggtt ttgcgcgcgc ggtaggcccg ggaccagcgg 
     1801 tctcggtcgt tgagggtcct gtgtattttt tccaggacgt ggtaaaggtg actctggatg 
     1861 ttcagataca tgggcataag cccgtctctg gggtggaggt agcaccactg cagagcttca 
     1921 tgctgcgggg tggtgttgta gatgatccag tcgtagcagg agcgctgggc gtggtgccta 
     1981 aaaatgtctt tcagtagcaa gctgattgcc aggggcaggc ccttggtgta agtgtttaca 
     2041 aagcggttaa gctgggatgg gtgcatacgt ggggatatga gatgcatctt ggactgtatt 
     2101 tttaggttgg ctatgttccc agccatatcc ctccggggat tcatgttgtg cagaaccacc 
     2161 agcacagtgt atccggtgca cttgggaaat ttgtcatgta gcttagaagg aaatgcgtgg 
     2221 aagaacttgg agacgccctt gtgacctcca agattttcca tgcattcgtc cataatgatg 
     2281 gcaatgggcc cacgggcggc ggcctgggcg aagatatttc tgggatcact aacgtcatag 
     2341 ttgtgttcca ggatgagatc gtcataggcc atttttacaa agcgcgggcg gagggtgcca 
     2401 gactgcggta taatggttcc atccggccca ggggcgtagt taccctcaca gatttgcatt 
     2461 tcccacgctt tgagttcaga tggggggatc atgtctacct gcggggcgat gaagaaaacg 
     2521 gtttccgggg taggggagat cagctgggaa gaaagcaggt tcctgagcag ctgcgactta 
     2581 ccgcagccgg tgggcccgta aatcacacct attaccgggt gcaactggta gttaagagag 
     2641 ctgcagctgc cgtcatccct gagcaggggg gccacttcgt taagcatgtc cctgactcgc 
     2701 atgttttccc tgaccaaatc cgccagaagg cgctcgccgc ccagcgatag cagttcttgc 
     2761 aaggaagcaa agtttttcaa cggtttgaga ccgtccgccg taggcatgct tttgagcgtt 
     2821 tgaccaagca gttccaggcg gtcccacagc tcggtcacct gctctacggc atctcgatcc 
     2881 agcatatctc ctcgtttcgc gggttggggc ggctttcgct gtacggcagt agtcggtgct 
     2941 cgtccagacg ggccagggtc atgtctttcc acgggcgcag ggtcctcgtc agcgtagtct 
     3001 gggtcacggt gaaggggtgc gctccgggct gcgcgctggc cagggtgcgc ttgaggctgg 
     3061 tcctgctggt gctgaagcgc tgccggtctt cgccctgcgc gtcggccagg tagcatttga 
     3121 ccatggtgtc atagtccagc ccctccgcgg cgtggccctt ggcgcgcagc ttgcccttgg 
     3181 aggaggcgcc gcacgagggg cagtgcagac ttttgagggc gtagagcttg ggcgcgagaa 
     3241 ataccgattc cggggagtag gcatccgcgc cgcaggcccc gcagacggtc tcgcattcca 
     3301 cgagccaggt gagctctggc cgttcggggt caaaaaccag gtttccccca tgctttttga 
     3361 tgcgtttctt acctctggtt tccatgagcc ggtgtccacg ctcggtgacg aaaaggctgt 
     3421 ccgtgtcccc gtatacagac ttgagaggct gcattaatga atcggccaac gcgcggggag 
     3481 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 
     3541 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 
     3601 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 
     3661 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 
     3721 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 
     3781 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 
     3841 gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct 
     3901 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 
     3961 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 
     4021 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 
     4081 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 
     4141 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 
     4201 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 
     4261 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 
     4321 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 
     4381 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 
     4441 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 
     4501 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 
     4561 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 
     4621 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 
     4681 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 
     4741 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 
     4801 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 
     4861 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 
     4921 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 
     4981 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 
     5041 ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt 
     5101 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 
     5161 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 
     5221 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 
     5281 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 
     5341 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 
     5401 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat 
     5461 gacattaacc tataaaaata ggcgtatcac tctaggcaaa atagcaccct cccgctccag 
     5521 aacaacatac agcgcttcac agcggcagcc taacagtcag ccttaccagt aaaaaagaaa 
     5581 acctattaaa aaaacaccac tcgacacggc accagctcaa tcagtcacag tgtaaaaaag 
     5641 ggccaagtgc agagcgagta tatataggac taaaaaatga cgtaacggtt aaagtccaca 
     5701 aaaaacaccc agaaaaccgc acgcgaacct acgcccagaa acgaaagcca aaaaacccac 
     5761 aacttcctca aatcgtcact tccgttttcc cacgttacgt aacttcccat tttaagaaaa 
     5821 ctacaattcc caacacatac aagttactcc gccctaaaac ctacgtcacc cgccccgttc 
     5881 ccacgccccg cgccacgtca caaactccac cccctcatta tcatattggc ttcaatccaa 
     5941 aataaggtat a 
//

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