| 质粒名称: | pDG136 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000589 |
| 产品用途: | Sleeping Beauty specific transposon containing constitutive expression units for MCP-3xNS3a(H1) and ZeoR (ITRSB-PhEF1a-MCP-(NS3a(H1))3-pA::PmPGK-ZeoR-pA-ITRSB). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | EF1a, mPGK |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | MCP-(NS3a(H1))3, ZeoR |
| 物种来源: | synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Multi-layered computational gene networks by engineered tristate logics
Shao J., Qiu X., Zhang L., Li S., Xue S., Si Y., Li Y., Jiang J., Wu Y., Xiong Q., Wang Y., Chen Q., Gao T., Zhu L., Wang H., Xie M.
Cell 187, 5064-5080.e14 (2024)
|
pDG136
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pDG136载体质粒基本信息
pDG136质粒图谱载体图谱和pDG136载体序列质粒序列多克隆位点信息
pDG136质粒载体简介
pDG136质粒序列载体序列
LOCUS pDG136 7367 bp DNA circular SYN 07-MAR-2022
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM recombinant plasmid
REFERENCE 1 (bases 1 to 7367)
AUTHORS 李怡霖
TITLE Direct Submission
JOURNAL Exported Jul 18, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7367
/mol_type="other DNA"
/organism="recombinant plasmid"
misc_feature 235..818
/label=P_CMV
/label=nonstandard type: promoter_const
misc_feature 235..614
/label=P_CMV_enhancer
/label=nonstandard type: promoter_element
misc_feature 615..818
/label=P_CMV_IE
/label=nonstandard type: promoter_element
primer_bind 769..789
/label=oMHS-CMV-F
misc_feature 829
/label=putative transcriptional start
promoter 863..882
/label=T7 promoter
primer_bind 897..941
/label=olyl337
CDS 923..1684
/codon_start=1
/label=FC
/translation="MYRMQLLSCIALSLALVTNSISAMVRSDKTHTCPPCPAPELLGGP
SVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYN
STYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRE
EMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKS
RWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK"
misc_feature 923..982
primer_bind 982..1006
/label=Hwtx-F
misc_feature 983..1684
primer_bind 983..1006
/label=olyl499
CDS 1685..1713
/codon_start=1
/label=linker
/translation="ASGSGGGGG"
primer_bind 1695..1738
/label=olyl499
misc_feature 1715..2416
/label=insert from IL2-hIgG1-Fc-linker-Adiponetin Seri...
/note="note=""Fragment extracted from
IL2-hIgG1-Fc-linker-Adiponetin SerialC.xdna [1487 nt] :
(EcoRI[1] / HindIII[1492])"
misc_feature 1715..2416
/label=ad
primer_bind 1715..1738
/label=Hwtx-F
CDS 2417..2428
/codon_start=1
/label=fcs
/translation="RIKR"
primer_bind 2424..2465
/label=olyl339
misc_feature 2429..2443
/label=GS Linker
primer_bind complement(2439..2463)
/label=olyl484
misc_feature 2450..2928
/label=ecDHFR(N18T/A19V)
primer_bind complement(2542..2564)
/label=ecDHFR-SEQ-R
primer_bind complement(2922..2949)
/label=olyl340
terminator 2964..3191
/label=bGH polyA
rep_origin 3237..3665
/label=f1 bacteriophage origin of replication
/label=f1 bacteriophage origin of replication; arrow _f1
ori
misc_feature 3679..4008
/label=P_SV40
/label=nonstandard type: promoter_const
rep_origin 3859..3994
/label=SV40 ORI
misc_feature 3911..3960
/label=P_SV40 TSS-region
CDS 4075..4869
/label=Translation 2136-2930
misc_feature 4075..4866
/label=Kan/neoR
/label=nonstandard type: resistance
primer_bind complement(5209..5229)
/label=M13-rev
misc_binding complement(5235..5257)
/label=LacO
promoter complement(5261..5291)
/label=lac promoter
rep_origin complement(5615..6200)
/direction=LEFT
/label=high-copy-number ColE1/pMB1/pBR322/pUC origin o
/label=high-copy-number ColE1/pMB1/pBR322/pUC origin of
_ori
CDS complement(6371..7231)
/label=Translation 5292-4432
misc_feature complement(6374..7231)
/label=AmpR
/label=nonstandard type: resistance
promoter complement(7232..7336)
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttgcca ccatgtacag gatgcaactc ctgtcttgca ttgcactaag
961 tcttgcactt gtcacgaact cgatatcggc catggttaga tctgacaaaa ctcacacatg
1021 cccaccgtgc ccagcacctg aactcctggg gggaccgtca gtcttcctct tccccccaaa
1081 acccaaggac accctcatga tctcccggac ccctgaggtc acatgcgtgg tggtggacgt
1141 gagccacgaa gaccctgagg tcaagttcaa ctggtacgtg gacggcgtgg aggtgcataa
1201 tgccaagaca aagccgcggg aggagcagta caacagcacg taccgtgtgg tcagcgtcct
1261 caccgtcctg caccaggact ggctgaatgg caaggagtac aagtgcaagg tctccaacaa
1321 agccctccca gcccccatcg agaaaaccat ctccaaagcc aaagggcagc cccgagaacc
1381 acaggtgtac accctgcccc catcccggga ggagatgacc aagaaccagg tcagcctgac
1441 ctgcctggtc aaaggcttct atcccagcga catcgccgtg gagtgggaga gcaatgggca
1501 gccggagaac aactacaaga ccacgcctcc cgtgctggac tccgacggct ccttcttcct
1561 ctacagcaag ctcaccgtgg acaagagcag gtggcagcag gggaacgtct tctcatgctc
1621 cgtgatgcac gaggctctgc acaaccacta cacgcagaag agcctctccc tgtctccggg
1681 taaagccagc ggaagtggcg gaggaggcgg atccatatcg gccatggtta gatctgacaa
1741 aactcacaca tgcccaccgt gcccagcacc tgaactcctg gggggaccgt cagtcttcct
1801 cttcccccca aaacccaagg acaccctcat gatctcccgg acccctgagg tcacatgcgt
1861 ggtggtggac gtgagccacg aagaccctga ggtcaagttc aactggtacg tggacggcgt
1921 ggaggtgcat aatgccaaga caaagccgcg ggaggagcag tacaacagca cgtaccgtgt
1981 ggtcagcgtc ctcaccgtcc tgcaccagga ctggctgaat ggcaaggagt acaagtgcaa
2041 ggtctccaac aaagccctcc cagcccccat cgagaaaacc atctccaaag ccaaagggca
2101 gccccgagaa ccacaggtgt acaccctgcc cccatcccgg gaggagatga ccaagaacca
2161 ggtcagcctg acctgcctgg tcaaaggctt ctatcccagc gacatcgccg tggagtggga
2221 gagcaatggg cagccggaga acaactacaa gaccacgcct cccgtgctgg actccgacgg
2281 ctccttcttc ctctacagca agctcaccgt ggacaagagc aggtggcagc aggggaacgt
2341 cttctcatgc tccgtgatgc acgaggctct gcacaaccac tacacgcaga agagcctctc
2401 cctgtctccg ggtaaaagga tcaagagggg aggaggatct ggagaattca tgatcagcct
2461 gatcgccgcc ctggccgtgg accacgtgat cggcatggag accgtgatgc cctggaacct
2521 gcccgccgac ctggcctggt tcaagagaaa caccctgaac aagcccgtga tcatgggcag
2581 acacacctgg gagagcatcg gcagacccct gcccggcaga aagaacatca tcctgagcag
2641 ccagcccagc accgacgaca gagtgacctg ggtgaagagc gtggacgagg ccatcgccgc
2701 ctgcggcgac gtgcccgaga tcatggtgat cggcggcggc agagtgtacg agcagttcct
2761 gcccaaggcc cagaagctgt acctgaccca catcgacgcc gaggtggagg gcgacaccca
2821 cttccccgac tacgagcccg acgactggga gagcgtgttc agcgagttcc acgacgccga
2881 cgcccagaac agccacagct actgcttcga gatcctggag agaagatagt ctagagggcc
2941 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
3001 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
3061 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
3121 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
3181 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
3241 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
3301 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
3361 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
3421 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
3481 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
3541 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
3601 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
3661 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
3721 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
3781 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
3841 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
3901 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
3961 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
4021 gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt
4081 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
4141 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
4201 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
4261 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
4321 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
4381 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
4441 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
4501 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
4561 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
4621 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
4681 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
4741 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
4801 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
4861 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
4921 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
4981 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
5041 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
5101 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
5161 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
5221 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
5281 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
5341 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
5401 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
5461 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
5521 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
5581 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
5641 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
5701 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
5761 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
5821 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
5881 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
5941 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
6001 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
6061 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
6121 gatccggcaa acaaaccacc gctggtagcg gtttttttgt ttgcaagcag cagattacgc
6181 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
6241 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
6301 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
6361 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
6421 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
6481 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
6541 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
6601 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
6661 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
6721 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
6781 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
6841 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
6901 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
6961 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
7021 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
7081 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
7141 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
7201 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
7261 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
7321 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
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