pSL685

LOCUS       pSL685               7714 bp DNA     circular SYN 14-JAN-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7714)
  AUTHORS   Westlake University
  TITLE     Direct Submission
  JOURNAL   Exported Jul 21, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7714
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     863..882
                     /label=T7
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    895..900
                     /label=NheI
     misc_feature    911..915
                     /label=New Feature
     misc_feature    911..915
                     /label=New Feature
     misc_feature    923..991
                     /label=Secretory (IgK)
                     /label=nonstandard type:  signal
     CDS             1010..1522
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     misc_feature    1043..1045
                     /label=cyt-STOP (TGG->TGA)
     CDS             1532..1588
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1589..1918
                     /label=mINS
     primer_bind     1878..1898
                     /label=202-WKG-632-F
     misc_feature    1934..1937
                     /label=linker
     misc_feature    1954..1966
                     /label=linker
     misc_feature    1972..1990
                     /label=MS2 box
     misc_feature    1996..2005
                     /label=linker
     misc_feature    2011..2029
                     /label=MS2 box
     misc_feature    2035..2044
                     /label=linker
     misc_feature    2035..2044
                     /label=linker
     misc_feature    2043..2073
                     /label=hairpin
     misc_feature    2049..2067
                     /label=MS2 box
     misc_feature    2072..2084
                     /label=linker
     misc_feature    2083..2113
                     /label=hairpin
     misc_feature    2089..2107
                     /label=MS2 box
     misc_feature    2117..2122
                     /label=linker
     misc_feature    2131..2146
                     /label=linker
     misc_feature    2152..2155
                     /label=linker
     misc_feature    2172..2184
                     /label=linker
     misc_feature    2190..2208
                     /label=MS2 box
     misc_feature    2214..2223
                     /label=linker
     misc_feature    2229..2247
                     /label=MS2 box
     misc_feature    2253..2262
                     /label=linker
     misc_feature    2253..2262
                     /label=linker
     misc_feature    2261..2291
                     /label=hairpin
     misc_feature    2267..2285
                     /label=MS2 box
     misc_feature    2290..2302
                     /label=linker
     misc_feature    2301..2331
                     /label=hairpin
     misc_feature    2307..2325
                     /label=MS2 box
     misc_feature    2335..2340
                     /label=linker
     misc_feature    2349..2364
                     /label=linker
     misc_feature    2370..2373
                     /label=linker
     misc_feature    2390..2402
                     /label=linker
     misc_feature    2408..2426
                     /label=MS2 box
     misc_feature    2432..2441
                     /label=linker
     misc_feature    2447..2465
                     /label=MS2 box
     misc_feature    2471..2480
                     /label=linker
     misc_feature    2471..2480
                     /label=linker
     misc_feature    2479..2509
                     /label=hairpin
     misc_feature    2485..2503
                     /label=MS2 box
     misc_feature    2508..2520
                     /label=linker
     misc_feature    2519..2549
                     /label=hairpin
     misc_feature    2525..2543
                     /label=MS2 box
     misc_feature    2553..2558
                     /label=linker
     misc_feature    2567..2582
                     /label=linker
     misc_feature    2609..2621
                     /label=linker
     misc_feature    2627..2645
                     /label=MS2 box
     misc_feature    2651..2660
                     /label=linker
     misc_feature    2666..2684
                     /label=MS2 box
     misc_feature    2690..2699
                     /label=linker
     misc_feature    2690..2699
                     /label=linker
     misc_feature    2698..2728
                     /label=hairpin
     misc_feature    2704..2722
                     /label=MS2 box
     misc_feature    2727..2739
                     /label=linker
     misc_feature    2738..2768
                     /label=hairpin
     misc_feature    2744..2762
                     /label=MS2 box
     misc_feature    2772..2777
                     /label=linker
     misc_feature    2786..2801
                     /label=linker
     misc_feature    2807..2819
                     /label=linker
     misc_feature    2825..2843
                     /label=MS2 box
     misc_feature    2849..2858
                     /label=linker
     misc_feature    2864..2882
                     /label=MS2 box
     misc_feature    2888..2897
                     /label=linker
     misc_feature    2888..2897
                     /label=linker
     misc_feature    2896..2926
                     /label=hairpin
     misc_feature    2902..2920
                     /label=MS2 box
     misc_feature    2925..2937
                     /label=linker
     misc_feature    2936..2966
                     /label=hairpin
     misc_feature    2942..2960
                     /label=MS2 box
     misc_feature    2970..2975
                     /label=linker
     misc_feature    2984..2999
                     /label=linker
     misc_feature    3005..3017
                     /label=linker
     misc_feature    3023..3041
                     /label=MS2 box
     misc_feature    3047..3056
                     /label=linker
     misc_feature    3062..3080
                     /label=MS2 box
     misc_feature    3086..3095
                     /label=linker
     misc_feature    3086..3095
                     /label=linker
     misc_feature    3094..3124
                     /label=hairpin
     misc_feature    3100..3118
                     /label=MS2 box
     misc_feature    3123..3135
                     /label=linker
     misc_feature    3134..3164
                     /label=hairpin
     misc_feature    3140..3158
                     /label=MS2 box
     misc_feature    3168..3173
                     /label=linker
     misc_feature    3182..3197
                     /label=linker
     misc_feature    3204..3279
                     /label=HHR Env140_nutR_H6
     misc_feature    complement(3212..3220)
                     /label=Stem III
     misc_feature    3223..3238
                     /label=Stem I
     terminator      3247..3261
                     /label=nutR-boxB
     misc_feature    3265..3273
                     /label=Stem III
     terminator      3308..3535
                     /label=bGH PA
     rep_origin      3581..4009
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3595..3619)
                     /label=184-WKG-599-R
     primer_bind     3880..3899
                     /label=189-WKG-612-F
     promoter        4023..4352
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             4419..5213
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4463..4490)
                     /label=188-WKG-610-R
     polyA_signal    5387..5508
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5553..5573)
                     /label=M13-rev
     primer_bind     complement(5557..5573)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_binding    complement(5579..5601)
                     /label=LacO
     protein_bind    5581..5597
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5605..5635)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        complement(5606..5635)
                     /label=lac
     protein_bind    5650..5671
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    5777..6717
                     /label=ColE1
                     /note="ColE1"
     CDS             complement(6718..7578)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7579..7683)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttgcca ccatgactag tgagacagac acactcctgc tatgggtact
      961 gctgctctgg gttccaggtt ccactggtga cgctagtggt ggttctggta tggtcttcac
     1021 actcgaagat ttcgttgggg actggcgaca gacagccggc tacaacctgg accaagtcct
     1081 tgaacaggga ggtgtgtcca gtttgtttca gaatctcggg gtgtccgtaa ctccgatcca
     1141 aaggattgtc ctgagcggtg aaaatgggct gaagatcgac atccatgtca tcatcccgta
     1201 tgaaggtctg agcggcgacc aaatgggcca gatcgaaaaa atttttaagg tggtgtaccc
     1261 tgtggatgat catcacttta aggtgatcct gcactatggc acactggtaa tcgacggggt
     1321 tacgccgaac atgatcgact atttcggacg gccgtatgaa ggcatcgccg tgttcgacgg
     1381 caaaaagatc actgtaacag ggaccctgtg gaacggcaac aaaattatcg acgagcgcct
     1441 gatcaacccc gacggctccc tgctgttccg agtaaccatc aacggagtga ccggctggcg
     1501 gctgtgcgaa cgcattctgg cggctagcgg agcaaccaac ttttccctgc tgaagcaggc
     1561 aggcgacgtg gaggagaatc ctggacccat ggccctgtgg atgcgcttcc tgcccctgct
     1621 ggccctgctc gtcctctggg agcccaagcc tgcccaggct tttgtcaaac agcacctttg
     1681 tggtcctcac ctggtggagg ctctgtacct ggtgtgtggg gaacgtggtt tcttctacac
     1741 acccaagtcc cgtcgtaaaa gggaggaccc gcaagtgcca caactggagc tgggtggagg
     1801 cccggaggcc ggggatcttc agaccttggc actggaggtt gcccggcaga agcgtggcat
     1861 tgtggatcag tgctgcacca gcatctgctc cctctaccaa ctggagaact actgcaactg
     1921 actcgaggga tccaacttga accggtaaga attgcaacaa acaaatcacc accgtgagga
     1981 tcacccacgg tggtgaaagc cttagcctag ccgtgaggat cacccacggt aggcacaaga
     2041 aattctaccc gtgaggatca cccacgggta gaatcacaaa gactacagcc gtgaggatca
     2101 cccacggctg tagactcaaa cttaaaaagg atatcttcag caaactgatc caacttgaac
     2161 cggtaagaat tgcaacaaac aaatcaccac cgtgaggatc acccacggtg gtgaaagcct
     2221 tagcctagcc gtgaggatca cccacggtag gcacaagaaa ttctacccgt gaggatcacc
     2281 cacgggtaga atcacaaaga ctacagccgt gaggatcacc cacggctgta gactcaaact
     2341 taaaaaggat atcttcagca aactgatcca acttgaaccg gtaagaattg caacaaacaa
     2401 atcaccaccg tgaggatcac ccacggtggt gaaagcctta gcctagccgt gaggatcacc
     2461 cacggtaggc acaagaaatt ctacccgtga ggatcaccca cgggtagaat cacaaagact
     2521 acagccgtga ggatcaccca cggctgtaga ctcaaactta aaaaggatat cttcagcaaa
     2581 ctgatcggag acgagtccgt ctcaaattgc aacaaacaaa tcaccaccgt gaggatcacc
     2641 cacggtggtg aaagccttag cctagccgtg aggatcaccc acggtaggca caagaaattc
     2701 tacccgtgag gatcacccac gggtagaatc acaaagacta cagccgtgag gatcacccac
     2761 ggctgtagac tcaaacttaa aaaggatatc ttcagcaaac tgaattgcaa caaacaaatc
     2821 accaccgtga ggatcaccca cggtggtgaa agccttagcc tagccgtgag gatcacccac
     2881 ggtaggcaca agaaattcta cccgtgagga tcacccacgg gtagaatcac aaagactaca
     2941 gccgtgagga tcacccacgg ctgtagactc aaacttaaaa aggatatctt cagcaaactg
     3001 aattgcaaca aacaaatcac caccgtgagg atcacccacg gtggtgaaag ccttagccta
     3061 gccgtgagga tcacccacgg taggcacaag aaattctacc cgtgaggatc acccacgggt
     3121 agaatcacaa agactacagc cgtgaggatc acccacggct gtagactcaa acttaaaaag
     3181 gatatcttca gcaaactgaa ttcgtttaaa ccctcctcgt ccggggctgt gtcgccccgc
     3241 tgacgagccc tgaaaaaggg cgaaacgagg aggactagtg ggcccgttta aacccgctga
     3301 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     3361 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     3421 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     3481 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     3541 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     3601 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     3661 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     3721 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     3781 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     3841 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     3901 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     3961 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     4021 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     4081 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     4141 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     4201 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     4261 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     4321 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     4381 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     4441 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     4501 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     4561 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
     4621 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     4681 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     4741 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     4801 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     4861 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     4921 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
     4981 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     5041 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     5101 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     5161 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     5221 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     5281 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     5341 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     5401 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     5461 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     5521 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     5581 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     5641 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     5701 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     5761 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     5821 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     5881 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     5941 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     6001 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     6061 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     6121 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     6181 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     6241 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     6301 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     6361 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     6421 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     6481 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     6541 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     6601 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     6661 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     6721 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     6781 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     6841 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     6901 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     6961 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     7021 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     7081 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     7141 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     7201 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     7261 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     7321 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     7381 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     7441 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     7501 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     7561 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     7621 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     7681 gcgcacattt ccccgaaaag tgccacctga cgtc
//