| 质粒名称: | pGreen2x35SLUC |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 108228 |
| 存储实验室: | Alejandro Ferrando |
|
载体骨架基本信息 |
|
| 载体名称: | pGreenII0800-LUC |
| 载体出品公司: | Hellens et al |
| 完整质粒大小: | 7921bp |
| 载体类型: | Plant Expression, Luciferase |
| 载体抗性: | Kanamycin |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | iRFP713 |
| 启动子: | 2x35S |
| 标签/融合蛋白: | Firefly Luciferase (C terminal on insert) |
| 克隆方法: | 未知 |
|
文献引用方法 |
|
| 材料和方法部分: | pGreen2x35SLUC was a gift from Alejandro Ferrando (Addgene plasmid # 108228 ; http://n2t.net/addgene:108228 ; RRID:Addgene_108228) |
| 参考文献部分: | Quantification of Xylem-Specific Thermospermine-Dependent Translation of SACL Transcripts with Dual Luciferase Reporter System. Solé-Gil A, Úrbez C, Ferrando A, Blázquez MA. Methods Mol Biol. 2024;2722:79-87. doi: 10.1007/978-1-0716-3477-6_6. 10.1007/978-1-0716-3477-6_6 PubMed 37897601 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pGreen2x35SLUC 7921 bp ds-DNA circular SYN 13-MAY-2021
DEFINITION Plant expression as translational fusions to FLUC with RLUC
normalization.
ACCESSION .
VERSION .
KEYWORDS pGreen2x35SLUC
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7921)
AUTHORS Alejandro R. Ferrando
TITLE Ferrando Lab Plant Expression Vectors
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 7921)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7921
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(5..21)
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5..21)
/label=pBluescriptSK
/note="For pBluescript vector"
CDS 53..1705
/codon_start=1
/gene="luc+"
/product="firefly luciferase"
/label=luciferase
/note="enhanced luc+ version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
primer_bind complement(119..137)
/label=LucNrev
/note="Firefly luciferase, reverse primer"
polyA_signal 1767..1943
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
misc_feature 1953..1977
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
rep_origin complement(2068..2656)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2148..2167)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(2827..3642)
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
primer_bind 3550..3569
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
primer_bind 3634..3653
/label=pENTR-R
/note="pENTR vectors, reverse primer"
mobile_element 3698..4465
/mobile_element_type="insertion sequence:IS1"
/note="prokaryotic transposable element"
rep_origin 4710..5145
/label=pSa ori
/note="origin of replication from bacterial plasmid pSa"
misc_feature 5272..5294
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA
(truncated)"
promoter 5356..5701
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
CDS 5720..6655
/codon_start=1
/product="luciferase from the anthozoan coelenterate
Renilla reniformis (sea pansy)"
/label=Rluc
/translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
polyA_signal 6710..6886
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
primer_bind 7035..7057
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 7049..7066
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 7050..7066
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 7076..7095
/label=T7
/note="T7 promoter, forward primer"
promoter 7076..7094
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 7119..7135
/label=pBluescriptKS
/note="For pBluescript vector"
primer_bind 7120..7136
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
promoter 7567..7911
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
ORIGIN
1 gggggatcca ctagttctag agcggccgcc accgcggtgg agatcgaatt ccatggaaga
61 cgccaaaaac ataaagaaag gcccggcgcc attctatccg ctggaagatg gaaccgctgg
121 agagcaactg cataaggcta tgaagagata cgccctggtt cctggaacaa ttgcttttac
181 agatgcacat atcgaggtgg acatcactta cgctgagtac ttcgaaatgt ccgttcggtt
241 ggcagaagct atgaaacgat atgggctgaa tacaaatcac agaatcgtcg tatgcagtga
301 aaactctctt caattcttta tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc
361 gcccgcgaac gacatttata atgaacgtga attgctcaac agtatgggca tttcgcagcc
421 taccgtggtg ttcgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaagct
481 cccaatcatc caaaaaatta ttatcatgga ttctaaaacg gattaccagg gatttcagtc
541 gatgtacacg ttcgtcacat ctcatctacc tcccggtttt aatgaatacg attttgtgcc
601 agagtccttc gatagggaca agacaattgc actgatcatg aactcctctg gatctactgg
661 tctgcctaaa ggtgtcgctc tgcctcatag aactgcctgc gtgagattct cgcatgccag
721 agatcctatt tttggcaatc aaatcattcc ggatactgcg attttaagtg ttgttccatt
781 ccatcacggt tttggaatgt ttactacact cggatatttg atatgtggat ttcgagtcgt
841 cttaatgtat agatttgaag aagagctgtt tctgaggagc cttcaggatt acaagattca
901 aagtgcgctg ctggtgccaa ccctattctc cttcttcgcc aaaagcactc tgattgacaa
961 atacgattta tctaatttac acgaaattgc ttctggtggc gctcccctct ctaaggaagt
1021 cggggaagcg gttgccaaga ggttccatct gccaggtatc aggcaaggat atgggctcac
1081 tgagactaca tcagctattc tgattacacc cgagggggat gataaaccgg gcgcggtcgg
1141 taaagttgtt ccattttttg aagcgaaggt tgtggatctg gataccggga aaacgctggg
1201 cgttaatcaa agaggcgaac tgtgtgtgag aggtcctatg attatgtccg gttatgtaaa
1261 caatccggaa gcgaccaacg ccttgattga caaggatgga tggctacatt ctggagacat
1321 agcttactgg gacgaagacg aacacttctt catcgttgac cgcctgaagt ctctgattaa
1381 gtacaaaggc tatcaggtgg ctcccgctga attggaatcc atcttgctcc aacaccccaa
1441 catcttcgac gcaggtgtcg caggtcttcc cgacgatgac gccggtgaac ttcccgccgc
1501 cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg attacgtcgc
1561 cagtcaagta acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg acgaagtacc
1621 gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga gagatcctca taaaggccaa
1681 gaagggcgga aagatcgccg tgtaattcta gagaattcgg tacgctgaaa tcaccagtct
1741 ctctctacaa atctatctct ctctattttc tccataaata atgtgtgagt agtttcccga
1801 taagggaaat tagggttctt atagggtttc gctcatgtgt tgagcatata agaaaccctt
1861 agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa
1921 aatccagtac taaaatccag atccactagc cttgacagga tatattggcg ggtaaactaa
1981 gtcgctgtat gtgtttgttt gagatctcat gtgagcaaaa ggccagcaaa aggccaggaa
2041 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
2101 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
2161 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
2221 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
2281 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
2341 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
2401 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
2461 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
2521 tatctgcgct ctgctgaagc cagttacctt cggaagaaga gttggtagct cttgatccgg
2581 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
2641 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
2701 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
2761 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgtgt aacattggtc
2821 tagtgattag aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt
2881 atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca
2941 gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat
3001 acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt
3061 gacgactgaa tccggtgaga atggcaaaag tttatgcatt tctttccaga cttgttcaac
3121 aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg
3181 tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta aaaggacaat tacaaacagg
3241 aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc
3301 aggatattct tctaatacct ggaatgctgt tttccctggg atcgcagtgg tgagtaacca
3361 tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag
3421 ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt
3481 cagaaacaac tctggcgcat cgggcttccc atacaatcgg tagattgtcg cacctgattg
3541 cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa
3601 tcgcggcctt gagcaagacg tttcccgttg aatatggctc ataacacccc ttgtattact
3661 gtttatgtaa gcagacagtt ttattgttca tgatgatggt gatgctgcca acttactgat
3721 ttagtgtatg atggtgtttt tgaggtgctc cagtggcttc tgtttctatc agctgtccct
3781 cctgttcagc tactgacggg gtggtgcgta acggcaaaag caccgccgga catcagcgct
3841 atctctgctc tcactgccgt aaaacatggc aactgcagtt cacttacacc gcttctcaac
3901 ccggtacgca ccagaaaatc attgatatgg ccatgaatgg cgttggatgc cgggcaacag
3961 cccgcattat gggcgttggc ctcaacacga ttttacgtca cttaaaaaac tcaggccgca
4021 gtcggtaacc tcgcgcatac agccgggcag tgacgtcatc gtctgcgcgg aaatggacga
4081 acagtggggc tatgtcgggg ctaaatcgcg ccagcgctgg ctgttttacg cgtatgacag
4141 tctccggaag acggttgttg cgcacgtatt cggtgaacgc actatggcga cgctggggcg
4201 tcttatgagc ctgctgtcac cctttgacgt ggtgatatgg atgacggatg gctggccgct
4261 gtatgaatcc cgcctgaagg gaaagctgca cgtaatcagc aagcgatata cgcagcgaat
4321 tgagcggcat aacctgaatc tgaggcagca cctggcacgg ctgggacgga agtcgctgtc
4381 gttctcaaaa tcggtggagc tgcatgacaa agtcatcggg cattatctga acataaaaca
4441 ctatcaataa gttggagtca ttacccatga tgatatattt ttatcttgtg caatgtaaca
4501 tcagagattt tgagacacaa cgtggctttg ttgaataaat cgaacttttg ctgagttgaa
4561 ggatcagatc acgcatcttc ccgacaacgc agaccgttcc gtggcaaagc aaaagttcaa
4621 aatcaccaac tggtccacct acaacaaagc tctcatcaac cgtggctccc tcactttctg
4681 gctggatgat ggggcgattc aggcgatccc catccaacag cccgccgtcg agcgggcttt
4741 tttatccccg gaagcctgtg gatagagggt agttatccac gtgaaaccgc taatgccccg
4801 caaagccttg attcacgggg ctttccggcc cgctccaaaa actatccacg tgaaatcgct
4861 aatcagggta cgtgaaatcg ctaatcggag tacgtgaaat cgctaataag gtcacgtgaa
4921 atcgctaatc aaaaaggcac gtgagaacgc taatagccct ttcagatcaa cagcttgcaa
4981 acacccctcg ctccggcaag tagttacagc aagtagtatg ttcaattagc ttttcaatta
5041 tgaatatata tatcaattat tggtcgccct tggcttgtgg acaatgcgct acgcgcaccg
5101 gctccgcccg tggacaaccg caagcggttg cccaccgtcg agcgcctttg cccacaaccc
5161 ggcggccggc cgcaacagat cgttttataa attttttttt ttgaaaaaga aaaagcccga
5221 aaggcggcaa cctctcgggc ttctggattt ccgatccccg gaattagatc ttggcaggat
5281 atattgtggt gtaacgttat cgtaccccta ctccaaaaat gtcaaagata cagtctcaga
5341 agaccaaagg gctattgaga cttttcaaca aagggtaatt tcgggaaacc tcctcggatt
5401 ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag gtggctccta
5461 caaatgccat cattgcgata aaggaaaggc tatcattcaa gatgcctctg ccgacagtgg
5521 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac
5581 gtcttcaaag caagtggatt gatgtgacat ctccactgac gtaagggatg acgcacaatc
5641 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac
5701 agcccaagct ctagccacca tgacttcgaa agtttatgat ccagaacaaa ggaaacggat
5761 gataactggt ccgcagtggt gggccagatg taaacaaatg aatgttcttg attcatttat
5821 taattattat gattcagaaa aacatgcaga aaatgctgtt atttttttac atggtaacgc
5881 ggcctcttct tatttatggc gacatgttgt gccacatatt gagccagtag cgcggtgtat
5941 tataccagac cttattggta tgggcaaatc aggcaaatct ggtaatggtt cttataggtt
6001 acttgatcat tacaaatatc ttactgcatg gtttgaactt cttaatttac caaagaagat
6061 catttttgtc ggccatgatt ggggtgcttg tttggcattt cattatagct atgagcatca
6121 agataagatc aaagcaatag ttcacgctga aagtgtagta gatgtgattg aatcatggga
6181 tgaatggcct gatattgaag aagatattgc gttgatcaaa tctgaagaag gagaaaaaat
6241 ggttttggag aataacttct tcgtggaaac catgttgcca tcaaaaatca tgagaaagtt
6301 agaaccagaa gaatttgcag catatcttga accattcaaa gagaaaggtg aagttcgtcg
6361 tccaacatta tcatggcctc gtgaaatccc gttagtaaaa ggtggtaaac ctgacgttgt
6421 acaaattgtt aggaattata atgcttatct acgtgcaagt gatgatttac caaaaatgtt
6481 tattgaatcg gacccaggat tcttttccaa tgctattgtt gaaggtgcca agaagtttcc
6541 taatactgaa tttgtcaaag taaaaggtct tcatttttcg caagaagatg cacctgatga
6601 aatgggaaaa tatatcaaat cgttcgttga gcgagttctc aaaaatgaac aataattcta
6661 gggtacgctg aaatcaccag tctctctcta caaatctatc tctctctatt ttctccataa
6721 ataatgtgtg agtagtttcc cgataaggga aattagggtt cttatagggt ttcgctcatg
6781 tgttgagcat ataagaaacc cttagtatgt atttgtattt gtaaaatact tctatcaata
6841 aaatttctaa ttcctaaaac caaaatccag tactaaaatc cagatcgata acattaacgc
6901 ttacaatttc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
6961 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
7021 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata
7081 cgactcacta tagggcgaat tgggtaccgg gccccccctc gaggtcgacg gtatcgataa
7141 gcttgatatc gaattcctgc aggtcaacat ggtggagcac gacacacttg tctactccaa
7201 aaatatcaaa gatacagtct cagaagacca aagggcaatt gagacttttc aacaaagggt
7261 aatatccgga aacctcctcg gattccattg cccagctatc tgtcacttta ttgtgaagat
7321 agtggaaaag gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggccatcgt
7381 tgaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt
7441 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg ataacatggt
7501 ggagcacgac acacttgtct actccaaaaa tatcaaagat acagtctcag aagaccaaag
7561 ggcaattgag acttttcaac aaagggtaat atccggaaac ctcctcggat tccattgccc
7621 agctatctgt cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca
7681 tcattgcgat aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga
7741 tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa
7801 gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc
7861 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga cctcgagaat
7921 t
//
