| 质粒名称: | MP-P8 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 69667 |
| 存储实验室: | David Liu |
|
载体骨架基本信息 |
|
| 载体名称: | CloDF13 |
| 载体出品公司: | David Liu Lab |
| 载体抗性: | 氯霉素 |
| 空载体大小: | 2992 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | dnaQ926 dam seqA emrR ugi AID |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | E. coli PBAD |
| 标签/融合蛋白: | -- |
| 克隆方法: | Ligation Independent 克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | pBAD-F |
| 3' 测序引物 | CAT-F |
|
文献引用方法 |
|
| 材料和方法部分: | MP-P8 was a gift from David Liu (Addgene plasmid # 69667 ; http://n2t.net/addgene:69667 ; RRID:Addgene_69667) |
| 参考文献部分: | Development of potent in vivo mutagenesis plasmids with broad mutational spectra. Badran AH, Liu DR. Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425. 10.1038/ncomms9425 PubMed 26443021 |
买家导航
MPP8载体基本信息
MPP8载体图谱质粒图谱和多克隆位点信息
MPP8载体简介
MPP8载体序列
LOCUS MP-P8 6651 bp ds-DNA circular SYN 13-MAY-2021
DEFINITION Untargetted Bacterial Mutagenesis.
ACCESSION .
VERSION .
KEYWORDS MP-P8
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6651)
AUTHORS Badran AH, Liu DR
TITLE Development of potent in vivo mutagenesis plasmids with broad
mutational spectra.
JOURNAL Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425.
PUBMED 26443021
REFERENCE 2 (bases 1 to 6651)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6651
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin complement(39..777)
/direction=LEFT
/label=CloDF13 ori
/note="Plasmids containing the CloDF13 (CDF) origin of
replication can be propagated in E. coli cells that contain
additional plasmids with compatible origins."
terminator 867..912
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
CDS complement(927..1805)
/codon_start=1
/gene="araC"
/product="L-arabinose regulatory protein"
/label=araC
/translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
EKVNDVAVKLS"
promoter 1832..2116
/gene="araBAD"
/label=araBAD promoter
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction (Guzman et al., 1995)"
primer_bind 2039..2058
/label=pBAD Forward
/note="For vectors with E. coli araBAD promoter, forward
primer"
CDS 4924..5178
/codon_start=1
/product="uracil-DNA glycosylase inhibitor from a Bacillus
subtilis bacteriophage (Mol et al., 1995)"
/label=UGI
/translation="MTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHT
AYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
CDS 5208..5804
/codon_start=1
/gene="AICDA"
/product="human activation-induced cytidine deaminase
(Dickerson et al., 2003)"
/label=AID
/translation="MDSLLMNRRKFLYQFKNVRWAKGRRETYLCYVVKRRDSATSFSLD
FGYLRNKNGCHVELLFLRYISDWDLDPGRCYRVTWFTSWSPCYDCARHVADFLRGNPNL
SLRIFTARLYFCEDRKAEPEGLRRLHRAGVQIAIMTFKDYFYCWNTFVENHERTFKAWE
GLHENSVRLSRQLRRILLPLYEVDDLRDAFRTLGL"
terminator 5831..5862
/label=tonB terminator
/note="bidirectional E. coli tonB-P14 transcription
terminator"
CDS complement(5878..6537)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVTQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
1 cactcggtcg ctacgctccg ggcgtgagac tgcggcgggc gctgcggaca catacaaagt
61 tacccacaga ttccgtggat aagcagggga ctaacatgtg aggcaaaaca gcagggccgc
121 gccggtggcg tttttccata ggctccgccc tcctgccaga gttcacataa acagacgctt
181 ttccggtgca tctgtgggag ccgtgaggct caaccatgaa tctgacagta cgggcgaaac
241 ccgacaggac ttaaagatcc ccaccgtttc cggcgggtcg ctccctcttg cgctctcctg
301 ttccgaccct gccgtttacc ggatacctgt tccgcctttc tcccttacgg gaagtgtggc
361 gctttctcat agctcacaca ctggtatctc ggctcggtgt aggtcgttcg ctccaagctg
421 ggctgtaagc aagaactccc cgttcagccc gactgctgcg ccttatccgg taactgttca
481 cttgagtcca acccggaaaa gcacggtaaa acgccactgg cagcagccat tggtaactgg
541 gagttcgcag aggatttgtt tagctaaaca cgcggttgct cttgaagtgt gcgccaaagt
601 ccggctacac tggaaggaca gatttggttg ctgtgctctg cgaaagccag ttaccacggt
661 taagcagttc cccaactgac ttaaccttcg atcaaaccac ctccccaggt ggttttttcg
721 tttacagggc aaaagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
781 tctactgaac cgctctagat ttcagtgcaa tttatctctt caaatgtagc acctgaagtc
841 agcccaggag gaagaggaca tccggtcaaa taaaacgaaa ggctcagtcg aaagactggg
901 cctttcgttt tagacttagg gaccctttat gacaacttga cggctacatc attcactttt
961 tcttcacaac cggcacggaa ctcgctcggg ctggccccgg tgcatttttt aaatacccgc
1021 gagaaataga gttgatcgtc aaaaccaaca ttgcgaccga cggtggcgat aggcatccgg
1081 gtggtgctca aaagcagctt cgcctggctg atacgttggt cctcgcgcca gcttaagacg
1141 ctaatcccta actgctggcg gaaaagatgt gacagacgcg acggcgacaa gcaaacatgc
1201 tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt gatcgctgat gtactgacaa
1261 gcctcgcgta cccgattatc catcggtgga tggagcgact cgttaatcgc ttccatgcgc
1321 cgcagtaaca attgctcaag cagatttatc gccagcagct ccgaatagcg cccttcccct
1381 tgcccggcgt taatgatttg cccaaacagg tcgctgaaat gcggctggtg cgcttcatcc
1441 gggcgaaaga accccgtatt ggcaaatatt gacggccagt taagccattc atgccagtag
1501 gcgcgcggac gaaagtaaac ccactggtga taccattcgc gagcctccgg atgacgaccg
1561 tagtgatgaa tctctcctgg cgggaacagc aaaatatcac ccggtcggca aacaaattct
1621 cgtccctgat ttttcaccac cccctgaccg cgaatggtga gattgagaat ataacctttc
1681 attcccagcg gtcggtcgat aaaaaaatcg agataaccgt tggcctcaat cggcgttaaa
1741 cccgccacca gatgggcatt aaacgagtat cccggcagca ggggatcatt ttgcgcttca
1801 gccatacttt tcatactccc accattcaga gaagaaacca attgtccata ttgcatcaga
1861 cattgccgtc actgcgtctt ttactggctc ttctcgctaa cccaaccggt aaccccgctt
1921 attaaaagca ttctgtaaca aagcgggacc aaagccatga caaaaacgcg taacaaaagt
1981 gtctataatc acggcagaaa agtccacatt gattatttgc acggcgtcac actttgctat
2041 gccatagcat ttttatccat aagattagcg gatcctacct gacgcttttt atcgcaactc
2101 tctactgttt ctccataccc gtttttttgg acgcgtacaa ctcaagtctg acataaatga
2161 ccgctatgag cactgcaatt acacgccaga tcgttctcgc taccgcaacc accggtatga
2221 accagattgg tgcgcactat gaaggccaca agatcattga gattggtgcc gttgaagtgg
2281 tgaaccgtcg cctgacgggc aataacttcc atgtttatct caaacccgat cggctggtgg
2341 atccggaagc ctttggcgta catggtattg ccgatgaatt tttgctcgat aagcccacgt
2401 ttgccgaagt agccgatgag ttcatggact atattcgcgg cgcggagttg gtgatccata
2461 acgcagcgtt cgatatcggc tttatggact acgagttttc gttgcttaag cgcgatattc
2521 cgaagaccaa tactttctgt aaggtcaccg atagccttgc ggtggcgagg aaaatgtttc
2581 ccggtaagcg caacagcctc gatgcgttat gtgctcgcta cgaaatagat aacagtaaac
2641 gaacgctgca cggggcatta ctcgatgccc agatccttgc ggaagtttat ctggcgatga
2701 ccggtggtca aacgtcgatg gcttttgcga tggaaggaga gacacaacag caacaaggtg
2761 aagcaacaat tcagcgcatt gtacgtcagg caagtaagtt acgcgttgtt tttgcgacag
2821 atgaagagat tgcagctcat gaagcccgtc tcgatctggt gcagaagaaa ggcggaagtt
2881 gcctctggcg agcataattt aatatcagta aaccggacat aacccatgaa gaaaaatcgc
2941 gcttttttga agtgggcagg gggcaagtat cccctgcttg atgatattaa acggcatttg
3001 cccaagggcg aatgtctggt tgagcctttt gtaggtgccg ggtcggtgtt tctcaacacc
3061 gacttttctc gttatatcct tgccgatatc aatagcgacc tgatcagtct ctataacatt
3121 gtgaagatgc gtactgatga gtacgtacag gccgcacgcg agctgtttgt tcccgaaaca
3181 aattgcgccg aggtttacta tcagttccgc gaagagttca acaaaagcca ggatccgttc
3241 cgtcgggcgg tactgttttt atatttgaac cgctacggtt acaacggcct gtgtcgttac
3301 aatctgcgcg gtgagtttaa cgtgccgttc ggccgctaca aaaaacccta tttcccggaa
3361 gcagagttgt atcacttcgc tgaaaaagcg cagaatgcct ttttctattg tgagtcttac
3421 gccgatagca tggcgcgcgc agatgatgca tccgtcgtct attgcgatcc gccttatgca
3481 ccgctgtctg cgaccgccaa ctttacggcg tatcacacaa acagttttac gcttgaacaa
3541 caagcgcatc tggcggagat cgccgaaggt ctggttgagc gccatattcc agtgctgatc
3601 tccaatcacg atacgatgtt aacgcgtgag tggtatcagc gcgcaaaatt gcatgtcgtc
3661 aaagttcgac gcagtataag cagcaacggc ggcacacgta aaaaggtgga cgaactgctg
3721 gctttgtaca aaccaggagt cgtttcaccc gcgaaaaaat aattcagcta agacactgca
3781 ctggattaag atgaaaacga ttgaagttga tgatgaactc tacagctata ttgccagcca
3841 cactaagcat atcggcgaga gcgcatccga cattttacgg cgtatgttga aattttccgc
3901 cgcatcacag cctgctgctc cggtgacgaa agaggttcgc gttgcgtcac ctgctatcgt
3961 cgaagcgaag ccggtcaaaa cgattaaaga caaggttcgc gcaatgcgtg aacttctgct
4021 ttcggatgaa tacgcagagc aaaagcgagc ggtcaatcgc tttatgctgc tgttgtctac
4081 actatattct cttgacgccc aggcgtttgc cgaagcaacg gaatcgttgc acggtcgtac
4141 acgcgtttac tttgcggcag atgaacaaac gctgctgaaa aatggtaatc agaccaagcc
4201 gaaacatgtg ccaggcacgc cgtattgggt gatcaccaac accaacaccg gccgtaaatg
4261 cagcatgatc gaacacatca tgcagtcgat gcaattcccg gcggaattga ttgagaaggt
4321 ttgcggaact atctaacggc tgaaattaat gaggtcatac ccaaatggat agttcgttta
4381 cgcccattga acaaatgcta aaatttcgcg ccagccgcca cgaagatttt ccttatcagg
4441 agatccttct gactcgtctt tgcatgcaca tgcaaagcaa gctgctggag aaccgcaata
4501 aaatgctgaa ggctcaggga attaacgaga cgttgtttat ggcgttgatt acgctggagt
4561 ctcaggaaaa ccacagtatt cagccttctg aattaagttg tgctcttgga tcatcccgta
4621 ccaacgcgac gcgtattgcc gatgaactgg aaaaacgcgg ttggatcgaa cgtcgtgaaa
4681 gcgataacga tcgccgctgc ctgcatctgc aattaacgga aaaaggtcac gagtttttgc
4741 gcgaggtttt accaccgcag cataactgcc tgcatcaact ctggtccgcg ctcagcacaa
4801 cagaaaaaga tcagctcgag caaatcaccc gcaaattgct ctcccgtctc gaccagatgg
4861 aacaagacgg tgtggttctc gaagcgatga gctaataata caaaaattag gaggaatttc
4921 aacatgacaa atttatctga catcattgaa aaagaaacag gaaaacaact agtgattcaa
4981 gaatcaattc taatgttacc agaagaagta gaggaagtaa ttgggaataa accagaaagt
5041 gatattttag ttcatactgc ttatgatgaa agtacagatg aaaatgtaat gctattaact
5101 tcagatgctc cagaatataa accttgggct ttagtaattc aagacagtaa tggagaaaat
5161 aaaattaaaa tgttataagt cgagattaag taaaccggaa tctgaagatg gactctctgc
5221 tgatgaaccg tcgtaaattc ctgtaccagt tcaaaaacgt tcgttgggcg aaaggtcgtc
5281 gtgaaaccta cctgtgctac gttgttaaac gtcgtgactc tgcgacctct ttctctctgg
5341 acttcggtta cctgcgtaac aaaaacggtt gccacgttga actgctgttc ctgcgttaca
5401 tctctgactg ggacctggac ccgggtcgtt gctaccgtgt tacctggttc acctcttggt
5461 ctccgtgcta cgactgcgcg cgtcacgttg cggacttcct gcgtggtaac ccgaacctgt
5521 ctctgcgtat cttcaccgcg cgtctgtact tctgcgaaga ccgtaaagcg gaaccggaag
5581 gtctgcgtcg tctgcaccgt gcgggtgttc agatcgcgat catgaccttc aaagactact
5641 tctactgctg gaacaccttc gttgaaaacc acgaacgtac cttcaaagcg tgggaaggtc
5701 tgcacgaaaa ctctgttcgt ctgtctcgtc agctgcgtcg tatcctgctg ccgctgtacg
5761 aagttgacga cctgcgtgac gcgttccgta ccctgggtct gtaaacttaa ttaacggcac
5821 tcctcagcca agtcaaaagc ctccgaccgg aggcttttga ctacatgccc atggcgttta
5881 cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg
5941 gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc
6001 ttgcgtataa tatttgccca tagtgaaaac gggggcgaag aagttgtcca tattggccac
6061 gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc
6121 aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata
6181 tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc
6241 agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc
6301 gtctttcatt gccatacgga actccggatg agcattcatc aggcgggcaa gaatgtgaat
6361 aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc
6421 cagctgaacg gtctggttat aggtacattg agtaactgac tgaaatgcct caaaatgttc
6481 tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt
6541 agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat
6601 ttcattatgg tgaaagttgg aacctcttac gtgccaagcc aaataggccg t
//
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