pCMV-Tag 2C

价格:1250元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Stratagene
载体名称: pCMV-Tag 2C
质粒类型: 哺乳动物表达载体
高拷贝/低拷贝: 高拷贝
克隆方法: 多克隆位点;限制性内切酶
启动子: CMV
载体大小: 4325 bp
5' 测序引物及序列: CMV Forward:  CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列: --
载体标签: FLAG tag
载体抗性: Kanamycin.html' target='_blank'>卡那霉素
筛选标记: Neomycin
克隆菌株: XL1-Blue
宿主细胞(系): --
备注: FLAG标签含8个氨基酸残基(DYKDDDDK);
pCMV-Tag 2 A,B,C的差别仅在于读码框(阅读框)处。
产品目录号: 211172
稳定性: 瞬表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pCMV-Tag 2C载体图谱



pCMV-Tag 2C 多克隆位点

pCMV-Tag 2C 载体特征

载体简介

ADDITIONAL MATERIALS REQUIRED
T4 DNA ligase
Taq DNA polymerase
Taq DNA polymerase buffer
TE buffer
CIAP
Falcon 2059 polypropylene tubes (15-ml)
Water baths (37°C and 42°C)


XL1-Blue Genotype: recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F´ proAB lacIqZΔM15 Tn10 (Tetr)].
The XL1-Blue cells supplied with the pCMV-Tag vectors are not competent cells. Refer to Hanahan (1983) for a protocol for producing competent cells.

载体序列

LOCUS       pCMV-Tag 2C	4325 bp 	DNA	SYN
DEFINITION  pCMV-Tag 2C
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="pCMV-Tag 2C"
                     /mol_type="other DNA"
     promoter        16..568
                     /label="CMV_immearly_promoter"
     misc_feature    71..358
                     /label="CAG_enhancer"
     misc_feature    525..545
                     /label="CMV_fwd_primer"
     promoter        526..595
                     /label="CMV_promoter"
     promoter        620..639
                     /label="T3_promoter"
     misc_feature    complement(755..771)
                     /label="pBluescriptKS_primer"
     promoter        complement(827..845)
                     /label="T7_promoter"
     misc_feature    902..961
                     /label="SV40_3_splice"
     terminator      1012..1240
                     /label="SV40_PA_terminator"
     misc_feature    1210..1229
                     /label="EBV_rev_primer"
     rep_origin      complement(1379..1685)
                     /label="f1_origin"
     promoter        1764..1792
                     /label="AmpR_promoter"
     misc_feature    complement(1858..1878)
                     /label="pBABE_3_primer"
     misc_feature    complement(1864..2079)
                     /label="SV40_enhancer"
     promoter        1876..2144
                     /label="SV40_promoter"
     rep_origin      2043..2120
                     /label="SV40_origin"
     misc_feature    2105..2124
                     /label="SV40pro_F_primer"
     CDS             2227..3021
                     /label="ORF frame 1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     gene            2230..3018
                     /label="NeoR/KanR (variant)"
                     /gene="NeoR/KanR (variant)"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     CDS             complement(2536..3072)
                     /label="ORF frame 3"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKFFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMFFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     terminator      3196..3465
                     /label="TK_PA_terminator"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKFFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMFFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     rep_origin      3613..4232
                     /label="pBR322_origin"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKFFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMFFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
ORIGIN
    1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
   61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
  121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
  181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
  241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
  301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
  361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
  421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
  481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
  541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
  601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc
  661 gcggtggcgg ccgccaccat ggattacaag gatgacgacg ataagaagcc cgggcggatc
  721 ccccgggctg caggaattcg atatcaagct tatcgatacc gtcgacctcg agggggggcc
  781 cggtacctta attaattaag gtaccaggta agtgtaccca attcgcccta tagtgagtcg
  841 tattacaatt cactcgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggaga
  901 tccaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga
  961 ttcacagtcc caaggctcat ttcaggcccc tcagtcctca cagtctgttc atgatcataa
 1021 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
 1081 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
 1141 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
 1201 attctagttg tggtttgtcc aaactcatca atgtatctta acgcgtaaat tgtaagcgtt
 1261 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
 1321 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
 1381 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
 1441 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
 1501 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
 1561 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
 1621 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
 1681 aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
 1741 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
 1801 taaatgcttc aataatattg aaaaaggaag aatcctgagg cggaaagaac cagctgtgga
 1861 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
 1921 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
 1981 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
 2041 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
 2101 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
 2161 gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg
 2221 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
 2281 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
 2341 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
 2401 gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
 2461 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
 2521 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
 2581 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
 2641 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
 2701 gacgaagaac atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg
 2761 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
 2821 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
 2881 caggacatag cgttggctac ccgtgatatt gctgaagaac ttggcggcga atgggctgac
 2941 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
 3001 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc
 3061 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
 3121 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
 3181 tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa
 3241 cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc
 3301 ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt
 3361 ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag
 3421 gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat
 3481 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
 3541 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
 3601 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
 3661 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
 3721 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
 3781 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
 3841 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
 3901 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
 3961 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
 4021 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
 4081 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
 4141 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
 4201 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
 4261 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
 4321 ccgcc
//

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