pYC46

价格:1200元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品人: Irene Castano Navarro(2015)
载体名称: pYC46
质粒类型: 酿酒酵母表达载体;整合型载体;FLP/FRT系统载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: --
载体大小: 6017 bp
5' 测序引物及序列: M13 primer  5’TGTAAAACGACGGCCAGT 3’
3' 测序引物及序列: --
载体标签: 3XFLAG tag (C ter)
载体抗性: 氨苄青霉素
筛选标记: URA3,Nourseothricin
克隆菌株: DH10B感受态细胞 ,30°C
宿主细胞(系): 出芽酵母(酿酒酵母)、白色念珠菌
备注: --
产品目录号: 63904
稳定性: 瞬表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pYC46载体图谱



pYC46多克隆位点

载体简介

酿酒酵母整合型载体还有: 

pYC44
pYC56   红色荧光标签
pYC54   黄色荧光标签
pYC52   青色荧光标签
pYC50   13XMyc标签
pYC48   HA标签
pYC46   3XFlag标签



载体序列

LOCUS       pYC46	6017 bp 	DNA	SYN
DEFINITION  pYC46
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..6017
                     /organism="pYC46"
                     /mol_type="other DNA"
     misc_feature    complement(29..51)
                     /label="pGEX_3_primer"
     promoter        196..409
                     /label="URA3_promoter"
     gene            411..1211
                     /label="URA3"
                     /gene="URA3"
     CDS             411..1214
                     /label="ORF frame 3"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
                     LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
                     LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
                     TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
                     TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*"
     CDS             complement(446..922)
                     /label="ORF frame 2"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     misc_feature    complement(1814..1962)
                     /label="lacZ_a"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     misc_feature    1928..1950
                     /label="M13_pUC_fwd_primer"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     promoter        1943..1959
                     /label="M13_forward20_primer"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     promoter        1966..1984
                     /label="T7_promoter"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     misc_feature    2017..2033
                     /label="pBluescriptSK_primer"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     misc_feature    2052..2063
                     /label="EK"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     misc_feature    2112..2126
                     /label="EK"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     CDS             complement(2823..3326)
                     /label="ORF frame 1"
                     /translation="MNSWSINIGNFQPQMTSTSFSGEFSSQTHQSSTNTMTSMFWSNF
                     NIFNSQSSVPTRIRNNNETSQITIITISNESSRIWITIFTSIGFIIGFIIWENLSQWW
                     INWNFSQSETITSSSNSKNSIGSERTIQSFNSFSITWNRSSVSISSIIQSSRHNCRID
                     PVVYVRM*"
     CDS             2862..3434
                     /label="ORF frame 3"
                     /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                     FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                     NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
                     GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
     promoter        complement(3756..3775)
                     /label="T3_promoter"
                     /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                     FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                     NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
                     GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
     promoter        complement(3792..3810)
                     /label="M13_reverse_primer"
                     /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                     FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                     NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
                     GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
     misc_feature    complement(3809..3831)
                     /label="M13_pUC_rev_primer"
                     /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                     FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                     NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
                     GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
     rep_origin      complement(4183..4802)
                     /label="pBR322_origin"
                     /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                     FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                     NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
                     GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
     gene            complement(4957..5817)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                     FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                     NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
                     GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
     CDS             complement(4957..5817)
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(5859..5887)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 Tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
   61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
  121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
  181 accacgcttt tcaattcaat tcatcatttt ttttttattc ttttttttga tttcggtttc
  241 tttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca
  301 gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat
  361 tcttaaccca actgcacaga acaaaaacct gcaggaaacg aagataaatc ATGTCGAAAG
  421 CTACATATAA GGAACGTGCT GCTACTCATC CTAGTCCTGT TGCTGCCAAG CTATTTAATA
  481 TCATGCACGA AAAGCAAACA AACTTGTGTG CTTCATTGGA TGTTCGTACC ACCAAGGAAT
  541 TACTGGAGTT AGTTGAAGCA TTAGGTCCCA AAATTTGTTT ACTAAAAACA CATGTGGATA
  601 TCTTGACTGA TTTTTCCATG GAGGGCACAG TTAAGCCGCT AAAGGCATTA TCCGCCAAGT
  661 ACAATTTTTT ACTCTTCGAA GACAGAAAAT TTGCTGACAT TGGTAATACA GTCAAATTGC
  721 AGTACTCTGC GGGTGTATAC AGAATAGCAG AATGGGCAGA CATTACGAAT GCACACGGTG
  781 TGGTGGGCCC AGGTATTGTT AGCGGTTTGA AGCAGGCGGC AGAAGAAGTA ACAAAGGAAC
  841 CTAGAGGCCT TTTGATGTTA GCAGAATTGT CATGCAAGGG CTCCCTATCT ACTGGAGAAT
  901 ATACTAAGGG TACTGTTGAC ATTGCGAAGA GCGACAAAGA TTTTGTTATC GGCTTTATTG
  961 CTCAAAGAGA CATGGGTGGA AGAGATGAAG GTTACGATTG GTTGATTATG ACACCCGGTG
 1021 TGGGTTTAGA TGACAAGGGA GACGCATTGG GTCAACAGTA TAGAACCGTG GATGATGTGG
 1081 TCTCTACAGG ATCTGACATT ATTATTGTTG GAAGAGGACT ATTTGCAAAG GGAAGGGATG
 1141 CTAAGGTAGA GGGTGAACGT TACAGAAAAG CAGGCTGGGA AGCATATTTG AGAAGATGCG
 1201 GCCAGCAAAA CTAAaaaact gtattataag taaatgcatg tatactaaac tcacaaatta
 1261 gagcttcaat ttaattatat cagttattac cctgcggtgt gaaataccgc acagatgcgt
 1321 aaggagaaaa taccgcatca ggaaattgta aacgttaata ttttgttaaa attcgcgtta
 1381 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
 1441 aaatcaaaag aatagaccga gatagggttg agtgTTGTTC CAGTTTGGAA CAAGAGTCCA
 1501 CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC
 1561 CCACTACGTG AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA
 1621 AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG
 1681 GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG
 1741 GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG Cgccgctaca gggcgcgtcg
 1801 cgCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG
 1861 CTATTACGCC AGCTGGCGAA GGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA
 1921 GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTG AAttgtaata cgactcacta
 1981 tagggcgaat tggagctcca ccgcggtggc ggccgctcta gaactagtgg atcctaGGAG
 2041 CAGACTACAA AGATGACGAT GACAAaGgaG CCGATTATAA GGACGATGAC GATAAAGGGG
 2101 CTGACTACAA AGATGACGAT GACAAGtgat aagatccGAA GTTCCTATAC TTTCTAGAGA
 2161 ATAGGAACTT Cgtgcgcttt tgaaccacgt aaagtgctgt aaaCcagctc tattcaatga
 2221 ggtaaatgat aaagtttata agagaccaaa aaattcaaaa aaaaaattga aaaattatat
 2281 tggtatttat atgaaacaat agttcagttc aaagcgctag cttttctcat ctcaactaac
 2341 cggtattagg tgggtattta tttaatatgg gaacaagaaa tgtgacaTta atgttcatgt
 2401 ttttattttc attatttatt ctatatggtt tgggacttga tgttatgtct taattcatag
 2461 aGATCCgctt gcctcgtccc cgccgggtca cccggccagc gacatggagg cccagaatac
 2521 cctccttgac agtcttgacg tgcgcagctc aggggcatga tgtgactgtc gcccgtacat
 2581 ttagcccata catccccatg tataatcatt tgcatccata cattttgatg gccgcacggc
 2641 gcgaagcaaa aattacggct cctcgctgca gacctgcgag cagggaaacg ctcccctcac
 2701 agacgcgttg aattgtcccc acgccgcgcc cctgtagaga aatataaaag gttaggattt
 2761 gccactgagg ttcttctttc atatacttcc ttttaaaatc ttgctaggat acagttctca
 2821 catcacatcc gaacataaac aaccgGATCg atcctacaat tATGTCTACT ACTTTGGATG
 2881 ATACTGCTTA TAGATACAGA ACTTCTGTTC CAGGTGATGC TGAAGCTATT GAAGCTTTGG
 2941 ATGGTTCTTT CACTACCGAT ACTGTTTTTA GAGTTACTGC TACTGGTGAT GGTTTCACTT
 3001 TGAGAGAAGT TCCAGTTGAT CCACCATTGA CTAAGGTTTT CCCAGATGAT GAATCCGATG
 3061 ATGAATCCGA TGCTGGTGAA GATGGTGATC CAGATTCTAG AACTTTCGTT GCTTATGGTG
 3121 ATGATGGTGA TTTGGCTGGT TTCGTTGTTG TTTCTTATTC TGGTTGGAAC AGAAGATTGA
 3181 CTGTTGAAGA TATTGAAGTT GCTCCAGAAC ATAGAGGTCA TGGTGTTGGT AGAGCTTTGA
 3241 TGGGTTTGGC TACTGAATTC GCCAGAGAAA GAGGTGCTGG TCATTTGTGG TTGGAAGTTA
 3301 CCAATGTTAA TGCTCCAGCT ATTCATGCTT ATAGAAGAAT GGGTTTCACT TTGTGTGGTT
 3361 TGGATACTGC TTTATACGAT GGTACTGCTT CCGATGGTGA ACAAGCTTTG TATATGTCTA
 3421 TGCCATGTCC ATAAtaacTC GACcagtact gacaataaaa agattcttgt tttcaagaac
 3481 ttgtcatttg tatagttttt ttatattgta gttgttctat tttaatcaaa tgttagcgtg
 3541 atttatattt tttttcgcct cgacatcatc tgcccagatg cgaagttaag tgcgcagaaa
 3601 gtaatatcat gcgtcaatcg tatgtgaatg ctggtcgcta tactgctgtc gattcgatac
 3661 taacgccgcc atccagtgTC GAtcgacGAA GTTCCTATAC TTTCTAGAGA ATAGGAACTT
 3721 CGTCctcgag ggggggcccg gtaccagctt ttgttccctt tagtgagggt taattccgag
 3781 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
 3841 acacaacata ggagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgaggta
 3901 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
 3961 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
 4021 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
 4081 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
 4141 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gcCGCGTTGC TGGCGTTTTT
 4201 CCATAGGCTC GGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG
 4261 AAACCCGACA GGACTATAAA GATACCAGGC GTTCCCCCCT GGAAGCTCCC TCGTGCGCTC
 4321 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT
 4381 GGCGCTTTCT CAATGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA
 4441 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA
 4501 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA
 4561 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA
 4621 CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT
 4681 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT
 4741 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT
 4801 CTtttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
 4861 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
 4921 aatctaaagt atatatgagt aaacttggtc tgacagTTAC CAATGCTTAA TCAGTGAGGC
 4981 ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTGC CCGTCGTGTA
 5041 GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA
 5101 CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG
 5161 CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC
 5221 TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT
 5281 CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG
 5341 GCGAGTTACA TGATCCCCCA TGTTGTGAAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT
 5401 CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA
 5461 TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA
 5521 GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA
 5581 TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG
 5641 GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC
 5701 ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG
 5761 AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATact
 5821 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
 5881 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
 5941 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
 6001 cacgaggccc tttcgtc
//

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