| 出品公司: | Agilent |
|---|---|
| 载体名称: | pCT74-HygR-RFP |
| 别称: | pCT74-Hygro-RFP |
| 质粒类型: | ORF |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | ToxA |
| 复制子: | pUC |
| 载体大小: | 5.0kb |
| 载体抗性: | Amp |
| 筛选标记: | Hyg |
| 克隆菌株: | DH5 alpha; 等 |
| 宿主细胞（系）: | 丝状真菌 |
| 备注: | 蛋白表达 |
| 产品目录号: | #20246 |
| 温度: | 37 |
买家导航
pCT74-HygR-RFP载体质粒基本信息
pCT74-HygR-RFP质粒图谱载体图谱和pCT74-HygR-RFP载体序列质粒序列多克隆位点信息
pCT74-HygR-RFP质粒载体简介
pCT74-HygR-RFP质粒序列载体序列
LOCUS pCT74-HygR-RFP 5056 bp DNA circular SYN 17-JUL-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5056)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 17, 2024 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5056
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 19..35
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
CDS 434..1459
/codon_start=1
/product="hygromycin B phosphotransferase"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANILFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
E"
CDS complement(1500..2195)
/codon_start=1
/product="red fluorescent protein from Entacmaea
quadricolor"
/label=TurboRFP
/note="mammalian codon-optimized"
/translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
DLPSKLGHR"
promoter complement(2213..2231)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(2252..2268)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 2276..2292
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(2300..2330)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2345..2366
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(2654..3242)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3413..4273)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4274..4378)
/gene="bla"
/label=AmpR promoter
rep_origin complement(4404..4859)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 5001..5017
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 5024..5042
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 caccgcggtg gcggccgctc tagaactagt ggatcccccg ggctgcagga attcatcgat
61 accgtcgacg ttaactgata ttgaaggagc attttttggg cttggctgga gctagtggag
121 gtcaacaatg aatgcctatt ttggtttagt cgtccaggcg gtgagcacaa aatttgtgtc
181 gtttgacaag atggttcatt taggcaactg gtcagatcag ccccacttgt agcagtagcg
241 gcggcgctcg aagtgtgact cttattagca gacaggaacg aggacattat tatcatctgc
301 tgcttggtgc acgataactt ggtgcgtttg tcaagcaagg taagtggacg acccggtcat
361 accttcttaa gttcgccctt cctcccttta tttcagattc aatctgactt acctattcta
421 cccaagcatc caaatgaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat
481 cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc
541 tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg
601 tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga
661 agtgcttgac attggggagt tcagcgagag cctgacctat tgcatctccc gccgtgcaca
721 gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctcc agccggtcgc
781 ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt
841 cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga
901 tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca
961 ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca
1021 tgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga
1081 ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatcc tcttctggag
1141 gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct
1201 tgcaggatcg ccgcgcctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca
1261 gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat
1321 cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt
1381 ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg
1441 tccgagggca aaggaataga gtagatgccg accgggaacc agttaacgtc gacctcgagt
1501 catctgtgcc ccagtttgct agggaggtcg cagtacttgg ccacagccat ctcgtgctgc
1561 tcgacgtagg tctctttgtc ggcctccttg attctttcca gtctgtggtc cacgaagtgg
1621 aagccgggca tcttgaggtt cttagcgggt ttcttggatc tgtatgtggt cttgaaggag
1681 cagtgcaggt agcccccgcc cacgagcttc agggccatct ggctgtggcc tctcaggccg
1741 ccgtcagcgg ggtacagcat ctcggtgttg gcctcccagc cgcgtgtttt cttctgcatc
1801 acagggccgt tggatgggaa gttcaccccg ttgatcttga cgttgtagat gatgcagccg
1861 ttctggaagc tggtgtcctg ggtagcggtc agcacgcccc cgtcttcgta tgtggtgatt
1921 ctctcccatg tgaagccctc agggaaggac tgcttaaaga agtcggggat gccctgggtg
1981 tggttgatga aggctttgct gccgtacatg aagctggtag ccaggatgtc gaaggcgaag
2041 gggagagggc cgccctcgac caccttgatc ttcatggtct gggtgccctc gtagggcttg
2101 ccttcgccct cggatgtgca cttgaagtgg tggttgttca cggtgccctc catgtacagc
2161 ttcatgtgca tgttctcctt gatcagctcg ctcatggtac cagcttttgt tccctttagt
2221 gagggttaat ttcgagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
2281 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
2341 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
2401 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
2461 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
2521 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
2581 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
2641 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
2701 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
2761 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
2821 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
2881 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
2941 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
3001 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
3061 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
3121 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
3181 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
3241 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
3301 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
3361 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
3421 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
3481 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
3541 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
3601 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
3661 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
3721 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
3781 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
3841 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
3901 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
3961 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
4021 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
4081 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
4141 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
4201 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
4261 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
4321 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
4381 catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt
4441 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
4501 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
4561 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
4621 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
4681 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
4741 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
4801 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
4861 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
4921 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
4981 gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg actcactata
5041 gggcgaattg gagctc
//
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