| 质粒名称: | pCDNA3-PAX3-FOXO1/FKHR |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 115526 |
| 存储实验室: | Martine Roussel |
|
载体骨架基本信息 |
|
| 载体名称: | pCDNA3 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Pax3-FOXO1/FKHR |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 2300 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pCDNA3-PAX3-FOXO1/FKHR was a gift from Martine Roussel (Addgene plasmid # 115526 ; http://n2t.net/addgene:115526 ; RRID:Addgene_115526) |
| 参考文献部分: | The oncogenic potential of the Pax3-FKHR fusion protein requires the Pax3 homeodomain recognition helix but not the Pax3 paired-box DNA binding domain. Lam PY, Sublett JE, Hollenbach AD, Roussel MF. Mol Cell Biol. 1999 Jan;19(1):594-601. PubMed 9858583 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCDNA3-PAX3-FOXO1/FKHR 8100 bp ds-DNA circular SYN 27-SEP-2018
DEFINITION Mammalian expression construct .
ACCESSION .
VERSION .
KEYWORDS pCDNA3-PAX3-FOXO1/FKHR
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8100)
AUTHORS Lam PY, Sublett JE, Hollenbach AD, Roussel MF
TITLE The oncogenic potential of the Pax3-FKHR fusion protein requires the
Pax3 homeodomain recognition helix but not the Pax3 paired-box DNA
binding domain.
JOURNAL Mol Cell Biol. 1999 Jan;19(1):594-601.
PUBMED 9858583
REFERENCE 2 (bases 1 to 8100)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 27, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8100
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 172..551
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 552..755
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 706..726
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 800..819
/label=T7
/note="T7 promoter, forward primer"
promoter 800..818
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
promoter complement(3590..3608)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(3591..3608)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(3628..3645)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 3634..3858
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 3904..4332
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3991..4010)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4201..4222
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(4341..4361)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 4346..4675
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 4526..4661
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 4588..4607
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 4742..5536
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(4796..4815)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 5406..5425
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 5710..5831
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5747..5766)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5801..5820
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(5880..5896)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5880..5896)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5893..5915)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5904..5920
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5928..5958)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5973..5994
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6111..6128)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6282..6870)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6362..6381)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7041..7901)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7664..7683
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7902..8006)
/gene="bla"
/label=AmpR promoter
primer_bind complement(8081..8100)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga
61 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta
121 gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat
181 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
241 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
301 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
361 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
421 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
481 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
541 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
601 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
661 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
721 ggcgtgtacg gtgggaggtc tatataagca gagctctctg gctaactaga gaacccactg
781 cttactggct tatcgaaatt aatacgactc actataggga gacccaagct tggtaccgag
841 ctcggatcct gcactcaagg gactcctctg gagcctgtgg acttggatct attagcgccc
901 ccctccccgc gccccgcctc cctctctggc cttttttggg ggaggagctc tccgagatcc
961 ggagagttcc cgagggtcac ccgccgcact gtgctcgctt tttcgtctcg ccttcacctg
1021 gatataattt gcgagcgaag ctgcccccag gatgaccacg ctggccggcg ctgtgcccag
1081 gatgatgcgg cccggcccgg ggcagaatta cccacgcagc ggcttcccgc tggaagtgtc
1141 cacccctctt ggccagggcc gagtcaacca gctcggagga gtatttatca acggcaggcc
1201 tctgcccaac catatccgcc acaagatagt ggagatggcc caccatggca ttcggccttg
1261 cgtcatttct cgccagcttc gcgtgtccca tggttgcgtc tctaagatcc tgtgcaggta
1321 ccaggagaca ggctccatcc gacctggtgc catcggcggc agcaaaccca agcaggtgac
1381 aacgcctgac gtggagaaga aaattgagga atacaaaaga gagaacccgg gcatgtttag
1441 ctgggaaatc agagacaaat tgctcaagga cgcggtctgt gatcgaaaca ccgtgccgtc
1501 agtgagttcc atcagccgca tcctgagaag taaattcggg aaaggtgaag aggaggaggc
1561 cgacttggag aggaaggagg cagaggaaag cgagaagaag gccaaacaca gcatcgacgg
1621 catcctgagc gagcgagcct cagcacccca atcagatgaa ggctctgata ttgactctga
1681 accagattta ccactaaaga ggaaacagcg cagaagccga accaccttca cagcagaaca
1741 gctggaggaa ctggagcgtg cttttgagag aactcattac cctgacattt atactaggga
1801 ggaactggcc cagagggcga agctcaccga ggcccgagta caggtctggt ttagcaaccg
1861 ccgtgcaaga tggaggaagc aagctggggc caatcaactg atggctttca accatctcat
1921 tcccgggggg ttccctccca ctgccatgcc gaccttgcca acgtaccagc tgtcggagac
1981 ctcttaccag cccacatcta ttccacaagc tgtgtcagat cccagcagca ccgttcacag
2041 acctcaaccg cttcctccaa gcactgtaca ccaaagcacg attccttcca acccagacag
2101 cagctctgcc tactgcctcc ccagcaccag gcatggattt tccagctata cagacagctt
2161 tgtgcctccg tcggggccct ccaaccccat gaaccccacc attggcaatg gcctctcacc
2221 tcagaattca attcgtcata atctgtccct acacagcaag ttcattcgtg tgcagaatga
2281 aggaactgga aaaagttctt ggtggatgct caatccagag ggtggcaaga gcgggaaatc
2341 tcctaggaga agagctgcat ccatggacaa caacagtaaa tttgctaaga gccgaagccg
2401 agctgccaag aagaaagcat ctctccagtc tggccaggag ggtgctgggg acagccctgg
2461 atcacagttt tccaaatggc ctgcaagccc tggctctcac agcaatgatg actttgataa
2521 ctggagtaca tttcgccctc gaactagctc aaatgctagt actattagtg ggagactctc
2581 acccattatg accgaacagg atgatcttgg agaaggggat gtgcattcta tggtgtaccc
2641 gccatctgcc gcaaagatgg cctctacttt acccagtctg tctgagataa gcaatcccga
2701 aaacatggaa aatcttttgg ataatctcaa ccttctctca tcaccaacat cattaactgt
2761 ttcgacccag tcctcacctg gcaccatgat gcagcagacg ccgtgctact cgtttgcgcc
2821 accaaacacc agtttgaatt cacccagccc aaactaccaa aaatatacat atggccaatc
2881 cagcatgagc cctttgcccc agatgcctat acaaacactt caggacaata agtcgagtta
2941 tggaggtatg agtcagtata actgtgcgcc tggactcttg aaggagttgc tgacttctga
3001 ctctcctccc cataatgaca ttatgacacc agttgatcct ggggtagccc agcccaacag
3061 ccgggttctg ggccagaacg tcatgatggg ccctaattcg gtcatgtcaa cctatggcag
3121 ccaggcatct cataacaaaa tgatgaatcc cagctcccat acccaccctg gacatgctca
3181 gcagacatct gcagttaacg ggcgtcccct gccccacacg gtaagcacca tgccccacac
3241 ctcgggtatg aaccgcctga cccaagtgaa gacacctgta caagtgcctc tgccccaccc
3301 catgcagatg agtgccctgg ggggctactc ctccgtgagc agctgcaatg gctatggcag
3361 aatgggcctt ctccaccagg agaagctccc aagtgacttg gatggcatgt tcattgagcg
3421 cttagactgt gacatggaat ccatcattcg gaatgacctc atggatggag atacattgga
3481 ttttaacttt gacaatgtgt tgcccaacca aagcttccca cacagtgtca agacaacgac
3541 acatagctgg gtgtcaggct gactcgagca tgcatctaga gggccctatt ctatagtgtc
3601 acctaaatgc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
3661 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
3721 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
3781 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
3841 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
3901 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
3961 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
4021 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
4081 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
4141 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
4201 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
4261 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
4321 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
4381 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
4441 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
4501 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
4561 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
4621 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
4681 cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg
4741 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
4801 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
4861 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
4921 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
4981 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
5041 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
5101 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
5161 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
5221 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
5281 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
5341 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
5401 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
5461 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
5521 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
5581 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
5641 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
5701 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
5761 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
5821 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
5881 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
5941 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
6001 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
6061 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
6121 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
6181 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
6241 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
6301 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
6361 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
6421 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
6481 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
6541 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
6601 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
6661 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
6721 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
6781 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
6841 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
6901 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
6961 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
7021 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
7081 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
7141 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
7201 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
7261 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
7321 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
7381 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
7441 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
7501 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
7561 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
7621 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
7681 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
7741 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
7801 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
7861 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
7921 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
7981 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac
8041 ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg
//
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇：YE1-BE4max
- 下一篇：pNLF1W
