pRetroX-IRES-ZsGreen1

价格:1000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pRetroX-IRES-ZsGreen1
质粒类型: 哺乳动物细胞双顺反子逆病毒载体
高拷贝/低拷贝: 高拷贝
启动子: --
克隆方法: 多克隆位点,限制性内切酶
载体大小: 5953bp
5' 测序引物及序列: --
3' 测序引物及序列: IRES-R: CCTCACATTGCCAAAAGACG
载体标签: --
载体抗性: 氨苄
筛选标记: --
备注: 载体表达ZsGreen绿色荧光蛋白作为筛选标志,用来构建稳表达细胞系。
产品目录号: 632520
稳定性: 稳表达
组成型: 组成型
病毒/非病毒: 逆病毒

载体质粒图谱和多克隆位点信息

pRetroX-IRES-ZsGreen1载体图谱



pRetroX-IRES-ZsGreen1多克隆位点

RNAi-Ready pSIREN-RetroQ载体特征

载体简介

载体序列

LOCUS       pRetroX-IRES-ZsGreen1   5953 bp    DNA   circular            10-NOV-2009
COMMENT     Created by Clontech Laboratories Inc. 
COMMENT     Cat. No. 632520
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     LTR             1..592
                     /label=5'\MMLV\LTR
     misc_feature    662..1012
                     /label=Packaging\signal
     intron          1075..1308
                     /label=Intron\containing\splice\acceptor\site
     LTR             2718..3309
                     /label=3'\MMLV\LTR
     rep_origin      4162..4281
                     /label=pUC\origin
     CDS             complement(4489..5278)
                     /label=Amp(R)
     misc_feature    1361..1390
                     /label=MCS
     misc_feature    1967..2662
                     /label=ZsGreen1
     misc_feature    1392..1966
                     /label=IRES
     misc_feature    650..655
                     /label=Splice\donor\site
BASE COUNT     1404 a      1586 c      1556 g      1407 t 
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat 
       61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca gatggaacag 
      121 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa 
      181 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc 
      241 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag 
      301 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg 
      361 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgggctcaat 
      421 aaaagagccc acaacccctc actcggcgcg ccagtcctcc gattgactga gtcgcccggg 
      481 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggcct cgctgttcct 
      541 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct 
      601 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg 
      661 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct 
      721 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga 
      781 gttcggaaca cccggccgca accctgggag acgtcccagg gacttcgggg gccgtttttg 
      841 tggcccgacc tgagtccaaa aatcccgatc gttttggact ctttggtgca ccccccttag 
      901 aggagggata tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg 
      961 aatttttgct ttcggtttgg gaccgaagcc gcgccgcgcg tcttgtctgc tgcagcatcg 
     1021 ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc tgaaaatacg cgtgtcgagc 
     1081 ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac 
     1141 actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa 
     1201 tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt 
     1261 ttttttcttc catttcagcc gtcgtgaaaa ctacccctaa aagccaaaaa aatcgaattc 
     1321 ccaaacttaa gcttggtacc gagctcggat ctcacgtggg cccgcggccg cagatctatc 
     1381 gatggatccg tgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga 
     1441 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa 
     1501 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc 
     1561 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc 
     1621 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg 
     1681 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca 
     1741 accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tcacctcaag 
     1801 cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct 
     1861 ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaacg tctaggcccc 
     1921 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg cccagtccaa 
     1981 gcacggcctg accaaggaga tgaccatgaa gtaccgcatg gagggctgcg tggacggcca 
     2041 caagttcgtg atcaccggcg agggcatcgg ctaccccttc aagggcaagc aggccatcaa 
     2101 cctgtgcgtg gtggagggcg gccccttgcc cttcgccgag gacatcttgt ccgccgcctt 
     2161 catgtacggc aaccgcgtgt tcaccgagta cccccaggac atcgtcgact acttcaagaa 
     2221 ctcctgcccc gccggctaca cctgggaccg ctccttcctg ttcgaggacg gcgccgtgtg 
     2281 catctgcaac gccgacatca ccgtgagcgt ggaggagaac tgcatgtacc acgagtccaa 
     2341 gttctacggc gtgaacttcc ccgccgacgg ccccgtgatg aagaagatga ccgacaactg 
     2401 ggagccctcc tgcgagaaga tcatccccgt gcccaagcag ggcatcttga agggcgacgt 
     2461 gagcatgtac ctgctgctga aggacggtgg ccgcttgcgc tgccagttcg acaccgtgta 
     2521 caaggccaag tccgtgcccc gcaagatgcc cgactggcac ttcatccagc acaagctgac 
     2581 ccgcgaggac cgcagcgacg ccaagaacca gaagtggcac ctgaccgagc acgccatcgc 
     2641 ctccggctcc gccttgccct gagcggcttc gaggataaaa taaaagattt tatttagtct 
     2701 ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag cttaagtaac 
     2761 gccattttgc aaggcatgga aaaatacata actgagaata gagaagttca gatcaaggtc 
     2821 aggaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg 
     2881 ccccggctca gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt 
     2941 ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag 
     3001 ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg 
     3061 accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc 
     3121 tgctccccga gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat 
     3181 tgactgagtc gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt 
     3241 gtggtctcgc tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg 
     3301 tctttcattg aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct 
     3361 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg 
     3421 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct 
     3481 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 
     3541 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 
     3601 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 
     3661 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 
     3721 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 
     3781 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 
     3841 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 
     3901 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 
     3961 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 
     4021 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 
     4081 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 
     4141 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc 
     4201 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 
     4261 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 
     4321 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 
     4381 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 
     4441 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 
     4501 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 
     4561 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 
     4621 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 
     4681 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 
     4741 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 
     4801 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 
     4861 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 
     4921 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 
     4981 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 
     5041 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 
     5101 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 
     5161 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 
     5221 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 
     5281 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 
     5341 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 
     5401 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 
     5461 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa 
     5521 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg 
     5581 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca 
     5641 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc 
     5701 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt 
     5761 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg 
     5821 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag 
     5881 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag 
     5941 tgccaagctt atg 
//

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