YIplac211

价格:1000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: ATCC
载体名称: YIplac211
质粒类型: 酿酒酵母表达载体;酵母整合型载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: lacZ
载体大小: 3797 bp
5' 测序引物及序列: M13 Reverse:CAGGAAACAGCTATGAC
3' 测序引物及序列: M13/pUC Forward:CCCAGTCACGACGTTGTAAAACG
载体标签: --
载体抗性: 氨苄青霉素
筛选标记: URA3
表达菌株: 酿酒酵母、白色念珠菌
备注: --
产品目录号: #87591
稳定性: 稳表达
组成型/诱导型: 诱导型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

YIplac211载体图谱



YIplac211 多克隆位点

载体简介

载体序列

LOCUS       YIplac211	3797 bp 	DNA	SYN
DEFINITION  YIplac211
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="YIplac211"
                     /mol_type="other DNA"
     promoter        142..171
                     /label="lac_promoter"
     misc_feature    185..207
                     /label="M13_pUC_rev_primer"
     promoter        206..224
                     /label="M13_reverse_primer"
     promoter        complement(290..306)
                     /label="M13_forward20_primer"
     misc_feature    complement(299..321)
                     /label="M13_pUC_fwd_primer"
     misc_feature    634..656
                     /label="pGEX_3_primer"
     CDS             complement(774..1577)
                     /label="ORF frame 1"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
                     LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
                     LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
                     TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
                     TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*"
     gene            complement(777..1577)
                     /label="URA3"
                     /gene="URA3"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
                     LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
                     LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
                     TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
                     TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*"
     CDS             1066..1542
                     /label="ORF frame 1"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     promoter        1926..1954
                     /label="AmpR_promoter"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     gene            1996..2856
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     CDS             1996..2856
                     /label="ORF frame 1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      3011..3630
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
   61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
  121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
  181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
  241 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattca ctggccgtcg
  301 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
  361 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
  421 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
  481 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
  541 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
  601 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
  661 caccgtcatc accgaaacgc gcgagacgaa agggcgggta ataactgata taattaaatt
  721 gaagctctaa tttgtgagtt tagtatacat gcatttactt ataatacagt tttttagttt
  781 tgctggccgc atcttctcaa atatgcttcc cagcctgctt ttctgtaacg ttcaccctct
  841 accttagcat cccttccctt tgcaaatagt cctcttccaa caataataat gtcagatcct
  901 gtagagacca catcatccac ggttctatac tgttgaccca atgcgtctcc cttgtcatct
  961 aaacccacac cgggtgtcat aatcaaccaa tcgtaacctt catctcttcc acccatgtct
 1021 ctttgagcaa taaagccgat aacaaaatct ttgtcgctct tcgcaatgtc aacagtaccc
 1081 ttagtatatt ctccagtaga tagggagccc ttgcatgaca attctgctaa catcaaaagg
 1141 cctctaggtt cctttgttac ttcttctgcc gcctgcttca aaccgctaac aatacctggg
 1201 cccaccacac cgtgtgcatt cgtaatgtct gcccattctg ctattctgta tacacccgca
 1261 gagtactgca atttgactgt attaccaatg tcagcaaatt ttctgtcttc gaagagtaaa
 1321 aaattgtact tggcggataa tgcctttagc ggcttaactg tgccctccat ggaaaaatca
 1381 gtcaagatat ccacatgtgt ttttagtaaa caaattttgg gacctaatgc ttcaactaac
 1441 tccagtaatt ccttggtggt acgaacatcc aatgaagcac acaagtttgt ttgcttttcg
 1501 tgcatgatat taaatagctt ggcagcaaca ggactaggat gagtagcagc acgttcctta
 1561 tatgtagctt tcgacatgat ttatcttcgt ttcctgcatg tttttgttct gtgcagttgg
 1621 gttaagaata ctgggcaatt tcatgtttct tcaacactac atatgcgtat atataccaat
 1681 ctaagtctgt gctccttcct tcgttcttcc ttctgttcgg agattaccga atcaaaaaaa
 1741 tttcaaagaa accgaaatca aaaaaaagaa taaaaaaaaa atgatgaatt gaattgaaaa
 1801 gctggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
 1861 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
 1921 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
 1981 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
 2041 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
 2101 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
 2161 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
 2221 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
 2281 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
 2341 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
 2401 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
 2461 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
 2521 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
 2581 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
 2641 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
 2701 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
 2761 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
 2821 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
 2881 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
 2941 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
 3001 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
 3061 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
 3121 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
 3181 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
 3241 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
 3301 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
 3361 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
 3421 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
 3481 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
 3541 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
 3601 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
 3661 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
 3721 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
 3781 agcgaggaag cggaaga
//

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