GW1-pHRed

价格:1200元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

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载体基本信息

质粒名称: GW1-pHRed 
出品公司: 爱迪基因
目录编号: 31473
存储实验室: Gary Yellen

载体骨架基本信息
载体名称: GW1
载体出品公司: British Biotechnology
载体抗性: 氨苄霉素
空载体大小: 5300 bp
载体类型: 哺乳细胞表达
高拷贝/低拷贝: 未知
生长条件: 37 ℃

插入基因信息
插入基因名称: pHRed
物种来源: 合成
插入大小: 710 bp
克隆方法: 限制性内切酶
5' 克隆位点: XbaI(未破坏)
3' 克隆位点: NheI (未破坏)
5' 测序引物 CACAGCAATCGATCCATG
3' 测序引物  

文献引用方法
材料和方法部分: GW1-pHRed was a gift from Gary Yellen (Addgene plasmid # 31473)
参考文献部分: Imaging Intracellular pH in Live Cells with a Genetically Encoded Red Fluorescent Protein Sensor. Tantama M, Hung YP, Yellen G. J Am Chem Soc. 2011 Jul 6. 133(26):10034-7. 10.1021/ja202902d PubMed 21631110

载体图谱质粒图谱和多克隆位点信息

GW1-pHRed载体图谱

载体简介

载体序列

LOCUS       GW1-pHRed	5981 bp 	DNA	SYN
DEFINITION  GW1-pHRed
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..5981
                     /organism="GW1-pHRed"
                     /mol_type="other DNA"
     CDS             189..899
                     /label="ORF frame 3"
     terminator      1024..1155
                     /label="SV40_PA_terminator"
     misc_feature    1112..1131
                     /label="EBV_rev_primer"
     promoter        complement(1175..1193)
                     /label="M13_reverse_primer"
     misc_feature    complement(1192..1214)
                     /label="M13_pUC_rev_primer"
     promoter        complement(1228..1257)
                     /label="lac_promoter"
     rep_origin      complement(1566..2185)
                     /label="pBR322_origin"
     gene            complement(2340..3200)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(2340..3200)
                     /label="ORF frame 1"
     promoter        complement(3279..3307)
                     /label="AmpR_promoter"
     misc_feature    complement(3466..3488)
                     /label="pGEX_3_primer"
     misc_feature    complement(3675..3694)
                     /label="SV40pro_F_primer"
     misc_feature    complement(3725..3868)
                     /label="lacZ_a"
     CDS             complement(3725..4252)
                     /label="ORF frame 2"
     misc_feature    3839..3861
                     /label="M13_pUC_fwd_primer"
     promoter        3854..3870
                     /label="M13_forward20_primer"
     promoter        4420..4996
                     /label="CMV_immearly_promoter"
     misc_feature    4499..4786
                     /label="CAG_enhancer"
     misc_feature    4953..4973
                     /label="CMV_fwd_primer"
     promoter        4954..5023
                     /label="CMV_promoter"
     promoter        4966..5085
                     /label="CMV2_promoter"
     misc_feature    4997..5016
                     /label="pCEP_fwd_primer"
     misc_feature    4999..5023
                     /label="LNCX_primer"
     CDS             complement(5491..5949)
                     /label="ORF frame 3"
     CDS             complement(5852..920)
                     /label="ORF frame 1"
ORIGIN
    1 AAGCTTCCCG GCCACAGCAA TCGATCCATG ACACAACCAG TGACAAGCAG TCCCCCAGTG
   61 AAACCGCATC CGCATCCGAG TCCGATACCG ATAAAGATTC TGAATCGGAG TGAGTGCCGC
  121 GTCCGAGAGC GTTCCCTGTC CACGTCCACC ATCGGCGGAG CAGGTGTGCC TGAGGCCTCT
  181 AGACCACCAT GGTTTCCGTT ATCGCAAAGC AAATGACCTA TAAAGTTTAT ATGTCTGGCA
  241 CCGTTAACGG CCACTACTTC GAGGTTGAAG GTGACGGCAA GGGCAAGCCT TACGAGGGCG
  301 AGCAGACCGT TAAGCTGACC GTTACCAAAG GCGGCCCACT GCCTTTCGCC TGGGATATCC
  361 TGTCCCCTCA GCTCCAATAC GGCTCCATCC CTTTCACCAA ATACCCCGAG GACATCCCTG
  421 ACTACTTTAA GCAGAGCTTC CCAGAGGGCT ACACCTGGGA GCGCTCTATG AACTTCGAGG
  481 ACGGCGCAGT TTGTACCGTT TCCAACGACA GCTCCATCCA GGGTAACTGC TTCATCTACA
  541 ACGTTAAGAT TTCCGGCGAG AACTTCCCAC CTAACGGCCC AGTTATGCAG AAGAAAACCC
  601 AAGGCTGGGA GCCCAGCACC GAACGCCTGT TTGCCCGCGA CGGTATGCTG ATTGGTAACG
  661 ATTATATGGC CCTGAAACTG GAAGGCGGCG GTCACTACCT GTGCGAGTTC AAGAGCACTT
  721 ACAAGGCTAA AAAGCCTGTT CGCATGCCTG GCCGCCACGA AATCGACCGC AAGCTGGATG
  781 TTACCTCTCA CAACCGCGAC TACACCAGCG TTGAGCAGTG TGAAATCTCT ATCGCCCGCC
  841 ACTCTCTGCT GGGGACAGGC AACTCCGCCG ACGGTGGTGG CTCTGGTGGT GCTAGCTGAG
  901 TCGACGAATT CATTGATCAT AATCAGCCAT ACCACATTTG TAGAGGTTTT ACTTGCTTTA
  961 AAAAACCTCC CACACCTCCC CCTGAACCTG AAACATAAAA TGAATGCAAT TGTTGTTGTT
 1021 AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA
 1081 AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT
 1141 TATCATGTCT GGATCCGTAC CGAGCTCGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA
 1201 AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC
 1261 TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC
 1321 CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC
 1381 GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT
 1441 CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA
 1501 GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA
 1561 AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT
 1621 CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC
 1681 CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC
 1741 GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCAATGCT CACGCTGTAG GTATCTCAGT
 1801 TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC
 1861 CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG
 1921 CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA
 1981 GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC
 2041 GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA
 2101 ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA
 2161 GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC
 2221 TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA
 2281 AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT
 2341 TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA
 2401 GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC
 2461 AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC
 2521 CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG
 2581 TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC
 2641 GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC
 2701 AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG
 2761 GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC
 2821 ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT
 2881 GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC
 2941 TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC
 3001 ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC
 3061 AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC
 3121 GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA
 3181 CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATAAG CGGCCGCGGC CATGCCGGCC
 3241 ACTAGTCTCG AGTTATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT
 3301 ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT
 3361 GCCACCTGAC GTCTAAGAAA CCATTATTAT CATGACATTA ACCTATAAAA ATAGGCGTAT
 3421 CACGAGGCCC TTTCGTCTCG CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA
 3481 GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA
 3541 GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT AACTATGCGG CATCAGAGCA
 3601 GATTGTACTG AGAGTGCACC ATATGAAGAC GTCGCCTCCT CACTACTTCT GGAATAGCTC
 3661 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC GCGCCATTCG
 3721 CCATTCAGGC TGCGCAACTG TTGGGAAGGG CGATCGGTGC GGGCCTCTTC GCTATTACGC
 3781 CAGCTGGCGA AAGGGGGATG TGCTGCAAGG CGATTAAGTT GGGTAACGCC AGGGTTTTCC
 3841 CAGTCACGAC GTTGTAAAAC GACGGCCAGT GCCATCTGCA GTGAATAATA AAATGTGTGT
 3901 TTGTCCGAAA TACGCGTTTG AGATTTCTGT CCCGACTAAA TTCATGTCGC GCGATAGTGG
 3961 TGTTTATCGC CGATAGAGAT GGCGATATTG GAAAAATCGA TATTTGAAAA TATGGCATAT
 4021 TGAAAATGTC GCCGATGTGA GTTTCTGTGT AACTGATATC GCCATTTTTC CAAAAGTTGA
 4081 TTTTTGGGCA TACGCGATAT CTGGCGATAC GCTTATATCG TTTACGGGGG ATGGCGATAG
 4141 ACGCCTTTGG TGACTTGGGC GATTCTGTGT GTCGCAAATA TCGCAGTTTC GATATAGGTG
 4201 ACAGACGATA TGAGGCTATA TCGCCGATAG AGGCGACATC AAGCTGGCAC ATGGCCAATG
 4261 CATATCGATC TATACATTGA ATCAATATTG GCCATTAGCC ATATTATTCA TTGGTTATAT
 4321 AGCATAAATC AATATTGGCT ATTGGCCATT GCATACGTTG TATCCATATC ATAATATGTA
 4381 CATTTATATT GGCTCATGTC CAACATTACC GCCATGTTGA CATTGATTAT TGACTAGTTA
 4441 TTAATAGTAA TCAATTACGG GGTCATTAGT TCATAGCCCA TATATGGAGT TCCGCGTTAC
 4501 ATAACTTACG GTAAATGGCC CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC
 4561 AATAATGACG TATGTTCCCA TAGTAACGCC AATAGGGACT TTCCATTGAC GTCAATGGGT
 4621 GGAGTATTTA CGGTAAACTG CCCACTTGGC AGTACATCAA GTGTATCATA TGCCAAGTAC
 4681 GCCCCCTATT GACGTCAATG ACGGTAAATG GCCCGCCTGG CATTATGCCC AGTACATGAC
 4741 CTTATGGGAC TTTCCTACTT GGCAGTACAT CTACGTATTA GTCATCGCTA TTACCATGGT
 4801 GATGCGGTTT TGGCAGTACA TCAATGGGCG TGGATAGCGG TTTGACTCAC GGGGATTTCC
 4861 AAGTCTCCAC CCCATTGACG TCAATGGGAG TTTGTTTTGG CACCAAAATC AACGGGACTT
 4921 TCCAAAATGT CGTAACAACT CCGCCCCATT GACGCAAATG GGCGGTAGGC GTGTACGGTG
 4981 GGAGGTCTAT ATAAGCAGAG CTCGTTTAGT GAACCGTCAG ATCGCCTGGA GACGCCATCC
 5041 ACGCTGTTTT GACCTCCATA GAAGACACCG GGACCGATCC AGCCTCCGCG GCCGGGAACG
 5101 GTGCATTGGA ACGCGGATTC CCCGTGCCAA GAGTGACGTA AGTACCGCCT ATAGAGTCTA
 5161 TAGGCCCACC CCCTTGGCTT CTTATGCATG CTATACTGTT TTTGGCTTGG GGTCTATACA
 5221 CCCCCGCTTC CTCATGTTAT AGGTGATGGT ATAGCTTAGC CTATAGGTGT GGGTTATTGA
 5281 CCATTATTGA CCACTCCCCT ATTGGTGACG ATACTTTCCA TTACTAATCC ATAACATGGC
 5341 TCTTTGCCAC AACTCTCTTT ATTGGCTATA TGCCAATACA CTGTCCTTCA GAGACTGACA
 5401 CGGACTCTGT ATTTTTACAG GATGGGGTCT CATTTATTAT TTACAAATTC ACATATACAA
 5461 CACCACCGTC CCCAGTGCCC GCAGTTTTTA TTAAACATAA CGTGGGATCT CCACGCGAAT
 5521 CTCGGGTACG TGTTCCGGAC ATGGGCTCTT CTCCGGTAGC GGCGGAGCTT CTACATCCGA
 5581 GCCCTGCTCC CATGCCTCCA GCGACTCATG GTCGCTCGGC AGCTCCTTGC TCCTAACAGT
 5641 GGAGGCCAGA CTTAGGCACA GCACGATGCC CACCACCACC AGTGTGCCGC ACAAGGCCGT
 5701 GGCGGTAGGG TATGTGTCTG AAAATGAGCT CGGGGAGCGG GCTTGCACCG CTGACGCATT
 5761 TGGAAGACTT AAGGCAGCGG CAGAAGAAGA TGCAGGCAGC TGAGTTGTTG TGTTCTGATA
 5821 AGAGTCAGAG GTAACTCCCG TTGCGGTGCT GTTAACGGTG GAGGGCAGTG TAGTCTGAGC
 5881 AGTACTCGTT GCTGCCGCGC GCGCCACCAG ACATAATAGC TGACAGACTA ACAGACTGTT
 5941 CCTTTCCATG GGTCTTTTCT GCAGTCACCG TCCTTGACAC G
//

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