| 质粒名称: | pAAV2/5 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 104964 |
| 存储实验室: | Melina Fan |
|
载体骨架基本信息 |
|
| 载体名称: | pUC19 with additional elements |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | 7371 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达,AAV |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Rep2/Cap5 |
| 物种来源: | parvovirus |
| 插入大小: | 4058 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | p5 |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | M13 Reverse |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pAAV2/5 7371 bp ds-DNA circular SYN 25-SEP-2018
DEFINITION AAV packaging plasmid expressing Rep/Cap genes for production of
serotype 5.
ACCESSION .
VERSION .
KEYWORDS pAAV2/5
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7371)
TITLE AAV Packaging
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 7371)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 25, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7371
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 83..105
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 97..114
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 98..114
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(4593..4640)
/label=FRT
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
primer_bind complement(4654..4670)
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4654..4670)
/label=pBluescriptSK
/note="For pBluescript vector"
primer_bind complement(4720..4736)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4720..4736)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4733..4755)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4744..4760
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4768..4798)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4813..4834
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4951..4968)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5122..5710)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5202..5221)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5881..6741)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6504..6523
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6742..6846)
/gene="bla"
/label=AmpR promoter
rep_origin 6873..7328
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6960..6979)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 7169..7190
/label=F1ori-F
/note="F1 origin, forward primer"
ORIGIN
1 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
61 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagcgc
121 gccagcgctc gttcaaacct cccgctcaaa atggagaccc tgcgtgctca ctcgggctta
181 aatacccagc gtgaccacat ggtgtcgcaa aatgtcgcaa aacactcacg tgacctctaa
241 tacaggactc tccctaaccc tatgacgtaa ttcacgtcac gactccaccc ctccgcccgc
301 tgtttaaacg cccgggctgt agttaatgat taacccgcca tgctacttat ctacgtagtt
361 tattgattaa caagcattaa aggggtcggg taaggtatcg ggttccgata ggtctggtgg
421 ttctgtattc cccggtgctg tccggggcaa agtccacaaa ctgggggtcg ttgtagttgt
481 ttgtgtactg gatctctggg ttccacctct tggagttttc cttcttgagc tcccactcca
541 tctccacggt gacctgcccg gtgctgtact gggtgatgaa gctgctgacg ggcacgtccg
601 agaagctggt gatatttccg ggcacaggcg tgttcttgat gagcatcatg ggcggtgggt
661 gtttgagtcc gaatccgccc atggccggag aggggtgaaa gtgcgccccc gtctctggga
721 tcttggccca gatgggtcct tggaggtaca cgtccctctc catccacacg ctgccgggca
781 cgatttcctg gaggttgtac gtgccggtcg cgggggcagt ggtggagctc tggttgttgg
841 tggccatctg cccgccgacg ttgtacgcca cgcggttcac cggctgcgtc tcgctctcgc
901 tggtgatgag catgttgccc tcgaggtacg tggcggtggt gcccgggttc gccggctggc
961 tgttgaagat catagtgttc tccagggcat aggtgttgct gccctggagg ttgttggtca
1021 tgccgttcgg ctgcgggggc acctggtaac tcgcgccctc gagctccatc ctattggtcg
1081 tggcgaaggc gctgacactg gcgcggttga ccccggagcc caggttccag ccctgggttc
1141 ggcccatggg ccccgggaac cagtttttgt aggtgttggc gtatctcccg gccaggttct
1201 tgttgaactg gactccgcca gtgttatttg tgctcacgaa gcggtacaag tactggtcca
1261 ccagcgggtt ggccagcttg aacaggttct gactgggagc gaagctggag tggaagggca
1321 cctcctcaaa gttgtaggta aactcaaagt tgttgcccgt tctcagcatc ttgctgggaa
1381 agtactctag gcagaagaag ctgctcctct cggtgggatt ttctgtgttg tcgcggttca
1441 gcgtcgcgta accgtactgc ggcagcgtaa agacctgcgg agggaaggcc ggcaggcatc
1501 cctcggtccc gttgccgacg acgtagggca gctggtagtc gtcgtccgta aacacttgga
1561 cggtggaggt gaggttgttg gcgatggtgg tggtggagtc ctgcaccgtg acctctttga
1621 cttgaatgtt gaagattttg actctgaggg accggggtct gaagccccag tagttgttga
1681 tgagtctttg ccagtctcgg gggctccagt ggctgtggaa gcggttaaag tcaaagtacc
1741 cccagggggt gctgtatcca aagtaggcgt tggcgttgct tccgtcgacg gagccgcttt
1801 tgatctctcg gtactggtgg ttgttgtagc tgggcagcac ccaggttcgg gtggacttgg
1861 tgacgactct gtcccccatc cacgtggaat cgcaatgcca atctcccgag gcattgccca
1921 ctccatcggc accttggtta ttgtcgccca atgggccgcc acctcccgca gacattgtat
1981 cagctcccaa acttgaggct ggttgggctg ggatttgcag ctgctgggat ccgctgggtc
2041 cagcttcggc gtctgacgag gtggaaggct tggagtcctc ttcggtccga gccttctttc
2101 tttttggaaa gtggtcgtct atccgctttc cggtaggggc cgtcttagca ccctcttcaa
2161 ccaggccaaa aggttcgaga acccttttct tggcctgaaa gactgccttt ccgaggtttc
2221 ccccgaagga tgtgtcgtcg gcgagcttct cctgaaactc ggcgtccgcg tggttgtact
2281 tgaggtaggg gttgtctccc gcctcaagct gctcgttgta cgagatgtcg tgctctcgcg
2341 cgacctcgtc tgccctgttg acaggctctc ctcgatcgag accgtttccg ggtccgagat
2401 agttataacc aggcagcaca agaccacggg cttgatcttg atgctgctga ttgggttttg
2461 gtttcggtgg gcccgcttca aggcccaaaa actcgcgaag accttcacca acttcttcca
2521 accaatctgg agggtgatca acaaaagaca tacctgattt aaatcattta ttgttcaaag
2581 atgcagtcat ccaaatccac attgaccaga tcgcaggcag tgcaagcgtc tggcaccttt
2641 cccatgatat gatgaatgta gcacagtttc tgatacgcct ttttgacgac agaaacgggt
2701 tgagattctg acacgggaaa gcactctaaa cagtctttct gtccgtgagt gaagcagata
2761 tttgaattct gattcattct ctcgcattgt ctgcagggaa acagcatcag attcatgccc
2821 acgtgacgag aacatttgtt ttggtacctg tctgcgtagt tgatcgaagc ttccgcgtct
2881 gacgtcgatg gctgcgcaac tgactcgcgc acccgtttgg gctcacttat atctgcgtca
2941 ctgggggcgg gtcttttctt ggctccaccc tttttgacgt agaattcatg ctccacctca
3001 accacgtgat cctttgccca ccggaaaaag tctttgactt cctgcttggt gaccttccca
3061 aagtcatgat ccagacggcg ggtgagttca aatttgaaca tccggtcttg caacggctgc
3121 tggtgttcga aggtcgttga gttcccgtca atcacggcgc acatgttggt gttggaggtg
3181 acgatcacgg gagtcgggtc tatctgggcc gaggacttgc atttctggtc cacgcgcacc
3241 ttgcttcctc cgagaatggc tttggccgac tccacgacct tggcggtcat cttcccctcc
3301 tcccaccaga tcaccatctt gtcgacacag tcgttgaagg gaaagttctc attggtccag
3361 tttacgcacc cgtagaaggg cacagtgtgg gctatggcct ccgcgatgtt ggtcttcccg
3421 gtagttgcag gcccaaacag ccagatggtg ttcctcttgc cgaacttttt cgtggcccat
3481 cccagaaaga cggaagccgc atattgggga tcgtacccgt ttagttccaa aattttataa
3541 atccgattgc tggaaatgtc ctccacgggc tgctggccca ccaggtagtc gggggcggtt
3601 ttagtcaggc tcataatctt tcccgcattg tccaaggcag ccttgatttg ggaccgcgag
3661 ttggaggccg cattgaagga gatgtatgag gcctggtcct cctggatcca ctgcttctcc
3721 gaggtaatcc ccttgtccac gagccacccg accagctcca tgtacctggc tgaagttttt
3781 gatctgatca ccggcgcatc agaattggga ttctgattct ctttgttctg ctcctgcgtc
3841 tgcgacacgt gcgtcagatg ctgcgccacc aaccgtttac gctccgtgag attcaaacag
3901 gcgcttaaat actgttccat attagtccac gcccactgga gctcaggctg ggttttgggg
3961 agcaagtaat tggggatgta gcactcatcc accaccttgt tcccgcctcc ggcgccattt
4021 ctggtctttg tgaccgcgaa ccagtttggc aaagtcggct cgatcccgcg gtaaattctc
4081 tgaatcagtt tttcgcgaat ctgactcagg aaacgtccca aaaccatgga tttcaccccg
4141 gtggtttcca cgagcacgtg catgtggaag tagctctctc ccttctcaaa ttgcacaaag
4201 aaaagggcct ccggggcctt actcacacgg cgccattccg tcagaaagtc gcgctgcagc
4261 ttctcggcca cggtcagggg tgcctgctca atcagattca gatccatgtc agaatctggc
4321 ggcaactccc attccttctc ggccacccag ttcacaaagc tgtcagaaat gccgggcaga
4381 tgctcgtcaa ggtcgctggg gaccttaatc acaatctcgt aaaaccccgg cgtggcggct
4441 gcgcgttcaa acctcccgct tcaaaatgga gaccctgcgt gctcactcgg gcttaaatac
4501 ccagcgtgac cacatggtgt cgcaaaatgt cgcaaaacac tcacgtgacc tctaatacag
4561 gacctctagg ggtaccgagc tcgaatagat cagaagttcc tatactttct agagaatagg
4621 aacttcggaa taggaacttc tgatcttccg ggggatccac tagttctaga gcggccggcg
4681 cggggcgtta acgatatcgg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg
4741 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
4801 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
4861 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
4921 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
4981 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
5041 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
5101 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
5161 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
5221 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
5281 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
5341 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
5401 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
5461 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
5521 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
5581 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
5641 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
5701 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
5761 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
5821 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
5881 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
5941 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
6001 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
6061 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
6121 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
6181 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
6241 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
6301 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
6361 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
6421 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
6481 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
6541 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
6601 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
6661 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
6721 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
6781 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
6841 tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc
6901 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
6961 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
7021 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
7081 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
7141 tttgacgttg gagtccacgt tcttaatagt ggactcttgt tccaaactgg aacaacactc
7201 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
7261 ttaaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
7321 tacaatttcc attcgccatt caggctgcgc aactgttggg aagggcgatc g
//
