pLVTHM

价格:1000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: --
载体名称: pLVTHM
质粒类型: 哺乳动物细胞慢病毒表达载体, RNAi载体, Cre/loxP载体
高拷贝/低拷贝: 高拷贝
启动子: EF-1 alpha
克隆方法: 多克隆位点,限制性内切酶
载体大小: 11085 bp
5' 测序引物及序列: EF-1a Forward:   TCAAGCCTCAGACAGTGGTTC
3' 测序引物及序列: EGFP-N: CGTCGCCGTCCAGCTCGACCAG
载体标签: C-EGFP
载体抗性: 氨苄
备注: 载体使用Stbl3感受态细胞扩增质粒,可以减少载体基因重组几率。pLVTHM载体用来替代pLVTH,允许shRNA定向 克隆入载体。
产品目录号: --
稳定性: /
组成型: --
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLVTHM载体图谱


载体简介

pLVTHM allows for direct cloning of shRNAs into the lentiviral vectors and replaces the old pLVTH system from Trono lab.
pLVTHM is similar to pLVTH, but contains a 3bp substitution that generates a unique MluI site for direct cloning of an shRNA into MluI-ClaI.

Please note that there is an additional ClaI site in this vector that is blocked by Dam methylation (this site does not appear in the depositor's full sequence). The plasmid needs to be grown in a Dam+ bacterial strain in order to use ClaI for cloning.

Note that ClaI has lower salt concentration requirements than MluI. One way to handle this is to digest with ClaI for 45 minutes, followed by addition of diluted MluI buffer and MluI, then incubation for 90 more minutes. Be sure not to use a large amount of vector (1.7 ug to 2.5 ug is known to work).

Also, if your shRNA is already in pSUPER (or another plasmid under the control of the PolIII promoter) you may use the EcoRI-ClaI sites to replace the H1 promoter in pLVTHM with the H1-shRNA cassette from pSUPER (or another plasmid).

载体序列

LOCUS       pLVTHM	11085 bp 	DNA     circular	SYN
DEFINITION  pLVTHM
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..11085
                     /organism="pLVTHM"
                     /mol_type="other DNA"
     misc_feature    455..635
                     /label="truncHIV-1_3_LTR"
     misc_feature    455..635
                     /label="HIV-1_5_LTR"
     misc_feature    746..790
                     /label="HIV-1_psi_pack"
     CDS             1178..2056
                     /label="ORF frame 2"
     misc_feature    1300..1533
                     /label="RRE"
     CDS             complement(1906..3039)
                     /label="ORF frame 1"
     promoter        2129..3299
                     /label="EF1a_promoter"
     misc_feature    3247..3267
                     /label="EF1a_fwd_primer"
     misc_feature    3351..3366
                     /label="cPPT"
     CDS             3554..4291
                     /label="ORF frame 2"
     gene            3557..4270
                     /label="EGFP"
                     /gene="EGFP"
     misc_feature    complement(3599..3620)
                     /label="EGFP_N_primer"
     misc_feature    3737..3766
                     /label="Y66 (EGFP)"
     misc_feature    4207..4228
                     /label="EGFP_C_primer"
     misc_feature    4345..4932
                     /label="WPRE"
     CDS             complement(4430..4933)
                     /label="ORF frame 3"
     CDS             4433..4993
                     /label="ORF frame 2"
     misc_feature    5003..5018
                     /label="cPPT"
     misc_feature    5003..5024
                     /label="U3PPT"
     misc_feature    5085..5372
                     /label="TRE"
     misc_feature    5533..5552
                     /label="H1_primer"
     misc_feature    5647..5680
                     /label="loxP"
     misc_feature    5700..5880
                     /label="HIV-1_5_LTR"
     misc_feature    5700..5880
                     /label="truncHIV-1_3_LTR"
     misc_feature    complement(5906..5923)
                     /label="Sp6_primer"
     gene            6161..6424
                     /label="tet (300 - 563)"
                     /gene="tet (300 - 563)"
     misc_feature    complement(6213..6232)
                     /label="pBRrevBam_primer"
     misc_feature    6565..6587
                     /label="pGEX_3_primer"
     promoter        6746..6774
                     /label="AmpR_promoter"
     gene            6816..7676
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             6816..7676
                     /label="ORF frame 3"
     rep_origin      7831..8450
                     /label="pBR322_origin"
     misc_feature    complement(8676..8696)
                     /label="pBABE_3_primer"
     misc_feature    complement(8682..9135)
                     /label="SV40_enhancer"
     promoter        8694..8962
                     /label="SV40_promoter"
     rep_origin      8861..8938
                     /label="SV40_origin"
     misc_feature    8923..8942
                     /label="SV40pro_F_primer"
     misc_feature    10143..10158
                     /label="SV40_int"
     misc_feature    10164..10211
                     /label="SV40_3_splice"
     CDS             10288..10761
                     /label="ORF frame 1"
     misc_feature    10339..10359
                     /label="SV40 NLS"
     misc_feature    10345..10359
                     /label="NLS"
     terminator      10787..10918
                     /label="SV40_PA_terminator"
     misc_feature    10875..10894
                     /label="EBV_rev_primer"
ORIGIN
    1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
   61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
  121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
  181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
  241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
  301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
  361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
  421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
  481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
  541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
  601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
  661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
  721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
  781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
  841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
  901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
  961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
 1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
 1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
 1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
 1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
 1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
 1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
 1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
 1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
 1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
 1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
 1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
 1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
 1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
 1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
 1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
 1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
 1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgcgatgtcg acgataagct
 2041 ttgcaaagat ggataaagtt ttaaacagag aggaatcttt gcagctaatg gaccttctag
 2101 gtcttgaaag gagtgggaat tggctccggt gcccgtcagt gggcagagcg cacatcgccc
 2161 acagtccccg agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg
 2221 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg
 2281 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc
 2341 gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat
 2401 ggcccttgcg tgccttgaat tacttccact ggctgcagta cgtgattctt gatcccgagc
 2461 ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc
 2521 gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc
 2581 ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg
 2641 ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg
 2701 gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt
 2761 cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg
 2821 gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa
 2881 ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg
 2941 cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac
 3001 aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg
 3061 cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg
 3121 gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc
 3181 cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt
 3241 tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgag
 3301 gaatttcgac atttaaattt aattaatctc gacggtatcg gttaactttt aaaagaaaag
 3361 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
 3421 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttccgatc acgagactag
 3481 cctcgaggtt taaactacgg gatcttcgaa ggcctaagct tacgcgcgcg tcctagcgct
 3541 accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
 3601 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
 3661 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
 3721 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
 3781 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
 3841 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
 3901 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
 3961 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
 4021 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
 4081 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
 4141 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
 4201 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
 4261 gctgtacaag tccggactca gatctcgata gctagtagct agctagctag tcgagctcaa
 4321 cttcggggac tagtcatatg ataatcaacc tctggattac aaaatttgtg aaagattgac
 4381 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
 4441 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
 4501 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
 4561 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
 4621 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
 4681 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
 4741 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
 4801 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
 4861 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
 4921 cgcctccccg catcggtacg tatggccagg tacctttaag accaatgact tacaaggcag
 4981 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
 5041 aacgaagaca agatgggatc aattcaccat gctagtggat ccccctcgag tttaccactc
 5101 cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa
 5161 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
 5221 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
 5281 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
 5341 agtttaccac tccctatcag tgatagagaa aagtgaaagt cggggctgca ggaattcgaa
 5401 cgctgacgtc atcaacccgc tccaaggaat cgcgggccca gtgtcactag gcgggaacac
 5461 ccagcgcgcg tgcgccctgg caggaagatg gctgtgaggg acaggggagt ggcgccctgc
 5521 aatatttgca tgtcgctatg tgttctggga aatcaccata aacgtgaaat gtctttggat
 5581 ttgggaatct tataagttct gtatgagacc acgcgtctcc aagcttatcg ataccgtcgc
 5641 atgggaataa cttcgtatag catacattat acgaagttat gctgcttttt gcttgtactg
 5701 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
 5761 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
 5821 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
 5881 gcatctagaa ttaattccgt gtattctata gtgtcaccta aatcgtatgt gtatgataca
 5941 taaggttatg tattaattgt agccgcgttc taacgacaat atgtacaagc ctaattgtgt
 6001 agcatctggc ttactgaagc agaccctatc atctctctcg taaactgccg tcagagtcgg
 6061 tttggttgga cgaaccttct gagtttctgg taacgccgtc ccgcacccgg aaatggtcag
 6121 cgaaccaatc agcagggtca tcgctagcca gatcctctac gccggacgca tcgtggccgg
 6181 catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga
 6241 agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg
 6301 ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc
 6361 ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg
 6421 agagcgtcga atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
 6481 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
 6541 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
 6601 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
 6661 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
 6721 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
 6781 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
 6841 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
 6901 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
 6961 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
 7021 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
 7081 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
 7141 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
 7201 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
 7261 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
 7321 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
 7381 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
 7441 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
 7501 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
 7561 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
 7621 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
 7681 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
 7741 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
 7801 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
 7861 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
 7921 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
 7981 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
 8041 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
 8101 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
 8161 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
 8221 gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
 8281 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
 8341 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
 8401 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
 8461 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
 8521 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
 8581 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
 8641 tccccgcgcg ttggccgatt cattaatgca gctgtggaat gtgtgtcagt tagggtgtgg
 8701 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
 8761 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
 8821 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
 8881 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
 8941 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
 9001 cttttgcaaa aagcttggac acaagacagg cttgcgagat atgtttgaga ataccacttt
 9061 atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt
 9121 ttagtgcgcc agatctctat aatctcgcgc aacctatttt cccctcgaac actttttaag
 9181 ccgtagataa acaggctggg acacttcaca tgagcgaaaa atacatcgtc acctgggaca
 9241 tgttgcagat ccatgcacgt aaactcgcaa gccgactgat gccttctgaa caatggaaag
 9301 gcattattgc cgtaagccgt ggcggtctgt accgggtgcg ttactggcgc gtgaactggg
 9361 tattcgtcat gtcgataccg tttgtatttc cagctacgat cacgacaacc agcgcgagct
 9421 taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg atgacctggt
 9481 ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac
 9541 catcttcgca aaaccggctg gtcgtccgct ggttgatgac tatgttgttg atatcccgca
 9601 agatacctgg attgaacagc cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg
 9661 tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt ctttttaact tcaggcgggt
 9721 tacaatagtt tccagtaagt attctggagg ctgcatccat gacacaggca aacctgagcg
 9781 aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc gacgctagtc cgccgcttta
 9841 atcacggcgc acaaccgcct gtgcagtcgg cccttgatgg taaaaccatc cctcactggt
 9901 atcgcatgat taaccgtctg atgtggatct ggcgcggcat tgacccacgc gaaatcctcg
 9961 acgtccaggc acgtattgtg atgagcgatg ccgaacgtac cgacgatgat ttatacgata
10021 cggtgattgg ctaccgtggc ggcaactgga tttatgagtg ggccccggat ctttgtgaag
10081 gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct
10141 aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt
10201 gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat
10261 gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac
10321 tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct
10381 tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt
10441 gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat
10501 tctgtaacct ttataagtag gcataacagt tataatcata acatactgtt ttttcttact
10561 ccacacaggc atagagtgtc tgctattaat aactatgctc aaaaattgtg tacctttagc
10621 tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt gactagagat
10681 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
10741 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
10801 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
10861 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
10921 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac
10981 tgccaattac ctagtggttt catttactct aaacctgtga ttcctctgaa ttattttcat
11041 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtag
//

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