pPRIME-TREX-GFP-FF3

价格:1000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Addgene
载体名称: pPRIME-TREX-GFP-FF3
质粒类型: 慢病毒载体;RNA干扰载体;四环素诱导载体
高拷贝/低拷贝: 低拷贝
启动子: TREX
克隆方法: 多克隆位点,限制性内切酶
载体大小: 8600bp
5' 测序引物及序列: EGFP-C Foward: CATGGTCCTGCTGGAGTTCGTG
3' 测序引物及序列: --
载体标签: --
载体抗性: 氯霉素(Chloramphenicol)
筛选标记: EGFP
克隆宿主菌: Stbl3,在30度条件下进行质粒扩增。
备注:
3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a Tet-responsive
promoter (TREX) controlling expression of GFP and miR30-based shRNA targeting firefly luciferase
产品目录号: 11667
稳定性: 稳表达
组成型/诱导型: 诱导型
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pPRIME-TREX-GFP-FF3载体图谱

载体简介

载体序列

LOCUS       pPRIME-TREX-GFP-FF3	8600 bp 	DNA	SYN
DEFINITION  pPRIME-TREX-GFP-FF3
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..8600
                     /organism="pPRIME-TREX-GFP-FF3"
                     /mol_type="other DNA"
     promoter        239..803
                     /label="CMV_immearly_promoter"
     misc_feature    318..605
                     /label="CAG_enhancer"
     misc_feature    772..792
                     /label="CMV_fwd_primer"
     misc_feature    820..976
                     /label="truncHIV-1_3_LTR"
     misc_feature    820..976
                     /label="HIV-1_5_LTR"
     misc_feature    1087..1131
                     /label="HIV-1_psi_pack"
     CDS             1523..2410
                     /label="ORF frame 2"

     misc_feature    1645..1878
                     /label="RRE"

     misc_feature    2409..2424
                     /label="cPPT"

     promoter        2606..3182
                     /label="CMV_immearly_promoter"

     misc_feature    2685..2972
                     /label="CAG_enhancer"

     misc_feature    3139..3159
                     /label="CMV_fwd_primer"

     promoter        3140..3263
                     /label="CMV_promoter"

     misc_feature    3190..3229
                     /label="tetO"

     misc_feature    3239..3263
                     /label="LNCX_primer"

     CDS             3302..4021
                     /label="ORF frame 2"

     gene            3305..4018
                     /label="EGFP"
                     /gene="EGFP"

     misc_feature    complement(3347..3368)
                     /label="EGFP_N_primer"

     misc_feature    3485..3514
                     /label="Y66 (EGFP)"

     misc_feature    3955..3976
                     /label="EGFP_C_primer"

     gene            4546..5205
                     /label="CAT/CamR"
                     /gene="CAT/CamR"

     CDS             4546..5205
                     /label="ORF frame 1"

     misc_feature    5491..6078
                     /label="WPRE"

     CDS             5579..6133
                     /label="ORF frame 2"

     CDS             5592..6632
                     /label="ORF frame 3"

     misc_feature    complement(6081..6097)
                     /label="pBluescriptKS_primer"

     misc_feature    6269..6284
                     /label="cPPT"

     misc_feature    6269..6290
                     /label="U3PPT"

     misc_feature    6606..6786
                     /label="HIV-1_5_LTR"

     misc_feature    6606..6786
                     /label="truncHIV-1_3_LTR"

     gene            complement(7607..8467)
                     /label="Ampicillin"
                     /gene="Ampicillin"

     CDS             complement(7607..8467)
                     /label="ORF frame 2"

     promoter        complement(8509..8537)
                     /label="AmpR_promoter"

ORIGIN
    1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
   61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
  121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
  181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
  241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
  301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
  361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
  421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
  481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
  541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
  601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
  661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
  721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
  781 gcggtaggcg tgtacggtgg gaggtctata gaccagatct gagcctggga gctctctggc
  841 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
  901 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
  961 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
 1021 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
 1081 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
 1141 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
 1201 cagggggaaa gaaaaaatat aaataaacat atagtatggg caagcaggga gctagaacga
 1261 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
 1321 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
 1381 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
 1441 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgcgctgat
 1501 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
 1561 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
 1621 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
 1681 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
 1741 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
 1801 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
 1861 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
 1921 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
 1981 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
 2041 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
 2101 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
 2161 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
 2221 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
 2281 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
 2341 attagtgaac ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc
 2401 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
 2461 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
 2521 ttcgggttta ttacagggac agcagagatc cagtttggtt agtaccgggc ccgctctaga
 2581 atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa
 2641 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
 2701 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
 2761 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
 2821 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
 2881 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
 2941 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
 3001 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
 3061 ttgacgtcaa tgggagtttg ttttggaacc aaaatcaacg ggactttcca aaatgtcgta
 3121 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
 3181 gcagagctct ccctatcagt gatagagatc tccctatcag tgatagagat cgtcgacgag
 3241 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgttac cggtcgccac
 3301 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
 3361 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
 3421 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
 3481 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
 3541 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
 3601 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
 3661 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
 3721 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
 3781 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
 3841 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
 3901 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
 3961 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
 4021 gcggccgcaa gccttgttaa gtgctcgctt cggcagcaca tatactatgt ttgaatgagg
 4081 cttcagtact ttacagaatc gttgcctgca catcttggaa acacttgctg ggattacttc
 4141 ttcaggttaa cccaacagaa ggctcgagca accagaattc aaggggctac tttaggagca
 4201 attatcttgt ttactaaaac tgaatacctt gctatctctt tgatacattt ttacaaagct
 4261 gaattaaaat ggtataaatt aaatcacttt tttcaattgg aagactaatg cgtttaaaca
 4321 cgcggcgacg cgttcgaccg aataaaacct gtgacggaag atcacttcgc agaataaata
 4381 aatcctggtg tccctgttga taccgggaag ccctgggcca acttttggcg aaaatgagac
 4441 gttgatcggc acgtaagagg ttccaacttt caccataatg aaataagatc actaccgggc
 4501 gtattttttg agttgtcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
 4561 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
 4621 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
 4681 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
 4741 ctgatgaatg ctcatccgga attacgtatg gcaatgaaag acggtgagct ggtgatatgg
 4801 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc
 4861 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg
 4921 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc
 4981 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac
 5041 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg
 5101 ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt
 5161 aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt
 5221 tattggtgcc cttaaacgcc tggttgctac gcctgaataa gtgataataa gcggatgaat
 5281 ggcagaaatt cggatctcga ccgcgtttgg gcggtggctc cctgccacgc ggctccgaac
 5341 agaagctgat ctccgaagag gatctgacat gtgtttaaac ctcgacttaa ttaagtcgag
 5401 ggtcgacggt atcgataagc tcgcttcacg agatcatgtt taagggttcc ggttccacta
 5461 ggtacaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag
 5521 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
 5581 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
 5641 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
 5701 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
 5761 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
 5821 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
 5881 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
 5941 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
 6001 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
 6061 ttgggccgcc tccccgcatc gataccgtcg acctcgatcg agacctagaa aaacatggag
 6121 caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag
 6181 aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca
 6241 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
 6301 actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc
 6361 ctgattggca gaactacaca ccagggccag ggatcagata tccactgacc tttggatggt
 6421 gctacaagct agtaccagtt gagcaagaga aggtagaaga agccaatgaa ggagagaaca
 6481 cccgcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtattag
 6541 agtggaggtt tgacagccgc ctagcatttc atcacatggc ccgagagctg catccggact
 6601 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
 6661 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
 6721 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
 6781 ctagcagcat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
 6841 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
 6901 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
 6961 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
 7021 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
 7081 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
 7141 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
 7201 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
 7261 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
 7321 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
 7381 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
 7441 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
 7501 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
 7561 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
 7621 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
 7681 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
 7741 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
 7801 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
 7861 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
 7921 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
 7981 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
 8041 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
 8101 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
 8161 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
 8221 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
 8281 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
 8341 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
 8401 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
 8461 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
 8521 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
 8581 cccgaaaagt gccacctgac
//

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