PX387

价格:1000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Addgene
载体名称: PX387 或 PX387 Mycoplasma mobile Cas9
质粒类型: CRISPR-Cas9载体;基因敲除载体;昆虫细胞载体
高拷贝/低拷贝: 高拷贝
插入位点: 限制性内切酶,多克隆位点
启动子: CMV
载体大小: 10904 bp
5' 测序引物及序列: CMV Forward:CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列: C-CMV-24 (5'-TATTAGGACAAGGCTGGTGGGCAC-3')
载体标签: NLS (C ter);HA、NLS (N ter)
载体抗性: 氨苄青霉素
筛选标记: Neomycin
克隆菌株: Stbl3
宿主细胞(系): 昆虫细胞
备注: --
产品目录号: #68324
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 杆状病毒

载体质粒图谱和多克隆位点信息

PX387载体图谱



载体简介

载体序列

LOCUS       PX387 Mycoplasma mobile Cas9	10904 bp 	DNA	SYN
DEFINITION  PX387 Mycoplasma mobile Cas9
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..10904
                     /organism="PX387 Mycoplasma mobile Cas9"
                     /mol_type="other DNA"
     misc_feature    47..1001
                     /label="Ac_5_flank"
     CDS             complement(343..948)
                     /label="ORF frame 3"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     promoter        1023..1587
                     /label="CMV_immearly_promoter"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1090..1377
                     /label="CAG_enhancer"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1544..1564
                     /label="CMV_fwd_primer"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     promoter        1615..1633
                     /label="T7_promoter"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1633..1656
                     /label="lacO"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1772..1796
                     /label="attB1"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     CDS             1822..5595
                     /label="ORF frame 1"
                     /translation="MYPYDVPDYASPKKKRKVEASMYFYKNKENKLNKKVVLGLDLGI
                     ASVGWCLTDISQKEDNKFPIILHGVRLFETVDDSDDKLLNETRRKKRGQRRRNRRLFT
                     RKRDFIKYLIDNNIIELEFDKNPKILVRNFIEKYINPFSKNLELKYKSVTNLPIGFHN
                     LRKAAINEKYKLDKSELIVLLYFYLSLRGAFFDNPEDTKSKEMNKNEIEIFDKNESIK
                     NAEFPIDKIIEFYKISGKIRSTINLKFGHQDYLKEIKQVFEKQNIDFMNYEKFAMEEK
                     SFFSRIRNYSEGPGNEKSFSKYGLYANENGNPELIINEKGQKIYTKIFKTLWESKIGK
                     CSYDKKLYRAPKNSFSAKVFDITNKLTDWKHKNEYISERLKRKILLSRFLNKDSKSAV
                     EKILKEENIKFENLSEIAYNKDDNKINLPIINAYHSLTTIFKKHLINFENYLISNEND
                     LSKLMSFYKQQSEKLFVPNEKGSYEINQNNNVLHIFDAISNILNKFSTIQDRIRILEG
                     YFEFSNLKKDVKSSEIYSEIAKLREFSGTSSLSFGAYYKFIPNLISEGSKNYSTISYE
                     EKALQNQKNNFSHSNLFEKTWVEDLIASPTVKRSLRQTMNLLKEIFKYSEKNNLEIEK
                     IVVEVTRSSNNKHERKKIEGINKYRKEKYEELKKVYDLPNENTTLLKKLWLLRQQQGY
                     DAYSLRKIEANDVINKPWNYDIDHIVPRSISFDDSFSNLVIVNKLDNAKKSNDLSAKQ
                     FIEKIYGIEKLKEAKENWGNWYLRNANGKAFNDKGKFIKLYTIDNLDEFDNSDFINRN
                     LSDTSYITNALVNHLTFSNSKYKYSVVSVNGKQTSNLRNQIAFVGIKNNKETEREWKR
                     PEGFKSINSNDFLIREEGKNDVKDDVLIKDRSFNGHHAEDAYFITIISQYFRSFKRIE
                     RLNVNYRKETRELDDLEKNNIKFKEKASFDNFLLINALDELNEKLNQMRFSRMVITKK
                     NTQLFNETLYSGKYDKGKNTIKKVEKLNLLDNRTDKIKKIEEFFDEDKLKENELTKLH
                     IFNHDKNLYETLKIIWNEVKIEIKNKNLNEKNYFKYFVNKKLQEGKISFNEWVPILDN
                     DFKIIRKIRYIKFSSEEKETDEIIFSQSNFLKIDQRQNFSFHNTLYWVQIWVYKNQKD
                     QYCFISIDARNSKFEKDEIKINYEKLKTQKEKLQIINEEPILKINKGDLFENEEKELF
                     YIVGRDEKPQKLEIKYILGKKIKDQKQIQKPVKKYFPNWKKVNLTYMGEIFKK*"
     misc_feature    1825..1851
                     /label="HA"
                     /translation="MYPYDVPDYASPKKKRKVEASMYFYKNKENKLNKKVVLGLDLGI
                     ASVGWCLTDISQKEDNKFPIILHGVRLFETVDDSDDKLLNETRRKKRGQRRRNRRLFT
                     RKRDFIKYLIDNNIIELEFDKNPKILVRNFIEKYINPFSKNLELKYKSVTNLPIGFHN
                     LRKAAINEKYKLDKSELIVLLYFYLSLRGAFFDNPEDTKSKEMNKNEIEIFDKNESIK
                     NAEFPIDKIIEFYKISGKIRSTINLKFGHQDYLKEIKQVFEKQNIDFMNYEKFAMEEK
                     SFFSRIRNYSEGPGNEKSFSKYGLYANENGNPELIINEKGQKIYTKIFKTLWESKIGK
                     CSYDKKLYRAPKNSFSAKVFDITNKLTDWKHKNEYISERLKRKILLSRFLNKDSKSAV
                     EKILKEENIKFENLSEIAYNKDDNKINLPIINAYHSLTTIFKKHLINFENYLISNEND
                     LSKLMSFYKQQSEKLFVPNEKGSYEINQNNNVLHIFDAISNILNKFSTIQDRIRILEG
                     YFEFSNLKKDVKSSEIYSEIAKLREFSGTSSLSFGAYYKFIPNLISEGSKNYSTISYE
                     EKALQNQKNNFSHSNLFEKTWVEDLIASPTVKRSLRQTMNLLKEIFKYSEKNNLEIEK
                     IVVEVTRSSNNKHERKKIEGINKYRKEKYEELKKVYDLPNENTTLLKKLWLLRQQQGY
                     DAYSLRKIEANDVINKPWNYDIDHIVPRSISFDDSFSNLVIVNKLDNAKKSNDLSAKQ
                     FIEKIYGIEKLKEAKENWGNWYLRNANGKAFNDKGKFIKLYTIDNLDEFDNSDFINRN
                     LSDTSYITNALVNHLTFSNSKYKYSVVSVNGKQTSNLRNQIAFVGIKNNKETEREWKR
                     PEGFKSINSNDFLIREEGKNDVKDDVLIKDRSFNGHHAEDAYFITIISQYFRSFKRIE
                     RLNVNYRKETRELDDLEKNNIKFKEKASFDNFLLINALDELNEKLNQMRFSRMVITKK
                     NTQLFNETLYSGKYDKGKNTIKKVEKLNLLDNRTDKIKKIEEFFDEDKLKENELTKLH
                     IFNHDKNLYETLKIIWNEVKIEIKNKNLNEKNYFKYFVNKKLQEGKISFNEWVPILDN
                     DFKIIRKIRYIKFSSEEKETDEIIFSQSNFLKIDQRQNFSFHNTLYWVQIWVYKNQKD
                     QYCFISIDARNSKFEKDEIKINYEKLKTQKEKLQIINEEPILKINKGDLFENEEKELF
                     YIVGRDEKPQKLEIKYILGKKIKDQKQIQKPVKKYFPNWKKVNLTYMGEIFKK*"
     misc_feature    1855..1875
                     /label="SV40 NLS"
                     /translation="MYPYDVPDYASPKKKRKVEASMYFYKNKENKLNKKVVLGLDLGI
                     ASVGWCLTDISQKEDNKFPIILHGVRLFETVDDSDDKLLNETRRKKRGQRRRNRRLFT
                     RKRDFIKYLIDNNIIELEFDKNPKILVRNFIEKYINPFSKNLELKYKSVTNLPIGFHN
                     LRKAAINEKYKLDKSELIVLLYFYLSLRGAFFDNPEDTKSKEMNKNEIEIFDKNESIK
                     NAEFPIDKIIEFYKISGKIRSTINLKFGHQDYLKEIKQVFEKQNIDFMNYEKFAMEEK
                     SFFSRIRNYSEGPGNEKSFSKYGLYANENGNPELIINEKGQKIYTKIFKTLWESKIGK
                     CSYDKKLYRAPKNSFSAKVFDITNKLTDWKHKNEYISERLKRKILLSRFLNKDSKSAV
                     EKILKEENIKFENLSEIAYNKDDNKINLPIINAYHSLTTIFKKHLINFENYLISNEND
                     LSKLMSFYKQQSEKLFVPNEKGSYEINQNNNVLHIFDAISNILNKFSTIQDRIRILEG
                     YFEFSNLKKDVKSSEIYSEIAKLREFSGTSSLSFGAYYKFIPNLISEGSKNYSTISYE
                     EKALQNQKNNFSHSNLFEKTWVEDLIASPTVKRSLRQTMNLLKEIFKYSEKNNLEIEK
                     IVVEVTRSSNNKHERKKIEGINKYRKEKYEELKKVYDLPNENTTLLKKLWLLRQQQGY
                     DAYSLRKIEANDVINKPWNYDIDHIVPRSISFDDSFSNLVIVNKLDNAKKSNDLSAKQ
                     FIEKIYGIEKLKEAKENWGNWYLRNANGKAFNDKGKFIKLYTIDNLDEFDNSDFINRN
                     LSDTSYITNALVNHLTFSNSKYKYSVVSVNGKQTSNLRNQIAFVGIKNNKETEREWKR
                     PEGFKSINSNDFLIREEGKNDVKDDVLIKDRSFNGHHAEDAYFITIISQYFRSFKRIE
                     RLNVNYRKETRELDDLEKNNIKFKEKASFDNFLLINALDELNEKLNQMRFSRMVITKK
                     NTQLFNETLYSGKYDKGKNTIKKVEKLNLLDNRTDKIKKIEEFFDEDKLKENELTKLH
                     IFNHDKNLYETLKIIWNEVKIEIKNKNLNEKNYFKYFVNKKLQEGKISFNEWVPILDN
                     DFKIIRKIRYIKFSSEEKETDEIIFSQSNFLKIDQRQNFSFHNTLYWVQIWVYKNQKD
                     QYCFISIDARNSKFEKDEIKINYEKLKTQKEKLQIINEEPILKINKGDLFENEEKELF
                     YIVGRDEKPQKLEIKYILGKKIKDQKQIQKPVKKYFPNWKKVNLTYMGEIFKK*"
     misc_feature    1861..1875
                     /label="NLS"
                     /translation="MYPYDVPDYASPKKKRKVEASMYFYKNKENKLNKKVVLGLDLGI
                     ASVGWCLTDISQKEDNKFPIILHGVRLFETVDDSDDKLLNETRRKKRGQRRRNRRLFT
                     RKRDFIKYLIDNNIIELEFDKNPKILVRNFIEKYINPFSKNLELKYKSVTNLPIGFHN
                     LRKAAINEKYKLDKSELIVLLYFYLSLRGAFFDNPEDTKSKEMNKNEIEIFDKNESIK
                     NAEFPIDKIIEFYKISGKIRSTINLKFGHQDYLKEIKQVFEKQNIDFMNYEKFAMEEK
                     SFFSRIRNYSEGPGNEKSFSKYGLYANENGNPELIINEKGQKIYTKIFKTLWESKIGK
                     CSYDKKLYRAPKNSFSAKVFDITNKLTDWKHKNEYISERLKRKILLSRFLNKDSKSAV
                     EKILKEENIKFENLSEIAYNKDDNKINLPIINAYHSLTTIFKKHLINFENYLISNEND
                     LSKLMSFYKQQSEKLFVPNEKGSYEINQNNNVLHIFDAISNILNKFSTIQDRIRILEG
                     YFEFSNLKKDVKSSEIYSEIAKLREFSGTSSLSFGAYYKFIPNLISEGSKNYSTISYE
                     EKALQNQKNNFSHSNLFEKTWVEDLIASPTVKRSLRQTMNLLKEIFKYSEKNNLEIEK
                     IVVEVTRSSNNKHERKKIEGINKYRKEKYEELKKVYDLPNENTTLLKKLWLLRQQQGY
                     DAYSLRKIEANDVINKPWNYDIDHIVPRSISFDDSFSNLVIVNKLDNAKKSNDLSAKQ
                     FIEKIYGIEKLKEAKENWGNWYLRNANGKAFNDKGKFIKLYTIDNLDEFDNSDFINRN
                     LSDTSYITNALVNHLTFSNSKYKYSVVSVNGKQTSNLRNQIAFVGIKNNKETEREWKR
                     PEGFKSINSNDFLIREEGKNDVKDDVLIKDRSFNGHHAEDAYFITIISQYFRSFKRIE
                     RLNVNYRKETRELDDLEKNNIKFKEKASFDNFLLINALDELNEKLNQMRFSRMVITKK
                     NTQLFNETLYSGKYDKGKNTIKKVEKLNLLDNRTDKIKKIEEFFDEDKLKENELTKLH
                     IFNHDKNLYETLKIIWNEVKIEIKNKNLNEKNYFKYFVNKKLQEGKISFNEWVPILDN
                     DFKIIRKIRYIKFSSEEKETDEIIFSQSNFLKIDQRQNFSFHNTLYWVQIWVYKNQKD
                     QYCFISIDARNSKFEKDEIKINYEKLKTQKEKLQIINEEPILKINKGDLFENEEKELF
                     YIVGRDEKPQKLEIKYILGKKIKDQKQIQKPVKKYFPNWKKVNLTYMGEIFKK*"
     CDS             complement(2809..3273)
                     /label="ORF frame 3"
                     /translation="MQDVIVLVDFVGALLIGHKQFLALLLVERHQLGQVIFVTDQIVL
                     EINQMLLEDCGQRVVGVNDWQVNLIIVLVVCDLGQIFELNVFLLQDFLHGTLRIFVQK
                     PRQQNFSLQALGDVFVFVLPISQLICDVKHFSGEAVLWGSIQFLVVAALPDF*"
     CDS             complement(4405..4923)
                     /label="ORF frame 3"
                     /translation="MVEDVQLGQLIFLQFILVEELLDFLNLIGSIVQQVQFFDFLDSV
                     LAFVILSAVQCLIEQLGVFLSDDHPRKSHLIQLLVQFIQSINQQKVVEGGLFFELDVI
                     LLQIVQLSGFFPVIHVQALNPLKTPEVLRDDSDEVGILCMVAVEAPILDQDIVFHVIL
                     SFLSNQKVIAVD*"
     misc_feature    complement(5628..5645)
                     /label="Sp6_primer"
                     /translation="MVEDVQLGQLIFLQFILVEELLDFLNLIGSIVQQVQFFDFLDSV
                     LAFVILSAVQCLIEQLGVFLSDDHPRKSHLIQLLVQFIQSINQQKVVEGGLFFELDVI
                     LLQIVQLSGFFPVIHVQALNPLKTPEVLRDDSDEVGILCMVAVEAPILDQDIVFHVIL
                     SFLSNQKVIAVD*"
     misc_feature    5650..5674
                     /label="attB2"
                     /translation="MVEDVQLGQLIFLQFILVEELLDFLNLIGSIVQQVQFFDFLDSV
                     LAFVILSAVQCLIEQLGVFLSDDHPRKSHLIQLLVQFIQSINQQKVVEGGLFFELDVI
                     LLQIVQLSGFFPVIHVQALNPLKTPEVLRDDSDEVGILCMVAVEAPILDQDIVFHVIL
                     SFLSNQKVIAVD*"
     terminator      5686..6152
                     /label="hGH_PA_terminator"
                     /translation="MVEDVQLGQLIFLQFILVEELLDFLNLIGSIVQQVQFFDFLDSV
                     LAFVILSAVQCLIEQLGVFLSDDHPRKSHLIQLLVQFIQSINQQKVVEGGLFFELDVI
                     LLQIVQLSGFFPVIHVQALNPLKTPEVLRDDSDEVGILCMVAVEAPILDQDIVFHVIL
                     SFLSNQKVIAVD*"
     terminator      6158..6193
                     /label="T7_terminator"
                     /translation="MVEDVQLGQLIFLQFILVEELLDFLNLIGSIVQQVQFFDFLDSV
                     LAFVILSAVQCLIEQLGVFLSDDHPRKSHLIQLLVQFIQSINQQKVVEGGLFFELDVI
                     LLQIVQLSGFFPVIHVQALNPLKTPEVLRDDSDEVGILCMVAVEAPILDQDIVFHVIL
                     SFLSNQKVIAVD*"
     rep_origin      6310..6616
                     /label="f1_origin"
                     /translation="MVEDVQLGQLIFLQFILVEELLDFLNLIGSIVQQVQFFDFLDSV
                     LAFVILSAVQCLIEQLGVFLSDDHPRKSHLIQLLVQFIQSINQQKVVEGGLFFELDVI
                     LLQIVQLSGFFPVIHVQALNPLKTPEVLRDDSDEVGILCMVAVEAPILDQDIVFHVIL
                     SFLSNQKVIAVD*"
     misc_feature    complement(6730..6750)
                     /label="pBABE_3_primer"
                     /translation="MVEDVQLGQLIFLQFILVEELLDFLNLIGSIVQQVQFFDFLDSV
                     LAFVILSAVQCLIEQLGVFLSDDHPRKSHLIQLLVQFIQSINQQKVVEGGLFFELDVI
                     LLQIVQLSGFFPVIHVQALNPLKTPEVLRDDSDEVGILCMVAVEAPILDQDIVFHVIL
                     SFLSNQKVIAVD*"
     misc_feature    complement(6736..6951)
                     /label="SV40_enhancer"
                     /translation="MVEDVQLGQLIFLQFILVEELLDFLNLIGSIVQQVQFFDFLDSV
                     LAFVILSAVQCLIEQLGVFLSDDHPRKSHLIQLLVQFIQSINQQKVVEGGLFFELDVI
                     LLQIVQLSGFFPVIHVQALNPLKTPEVLRDDSDEVGILCMVAVEAPILDQDIVFHVIL
                     SFLSNQKVIAVD*"
     promoter        6748..7016
                     /label="SV40_promoter"
                     /translation="MVEDVQLGQLIFLQFILVEELLDFLNLIGSIVQQVQFFDFLDSV
                     LAFVILSAVQCLIEQLGVFLSDDHPRKSHLIQLLVQFIQSINQQKVVEGGLFFELDVI
                     LLQIVQLSGFFPVIHVQALNPLKTPEVLRDDSDEVGILCMVAVEAPILDQDIVFHVIL
                     SFLSNQKVIAVD*"
     rep_origin      6915..6992
                     /label="SV40_origin"
                     /translation="MVEDVQLGQLIFLQFILVEELLDFLNLIGSIVQQVQFFDFLDSV
                     LAFVILSAVQCLIEQLGVFLSDDHPRKSHLIQLLVQFIQSINQQKVVEGGLFFELDVI
                     LLQIVQLSGFFPVIHVQALNPLKTPEVLRDDSDEVGILCMVAVEAPILDQDIVFHVIL
                     SFLSNQKVIAVD*"
     misc_feature    6977..6996
                     /label="SV40pro_F_primer"
                     /translation="MVEDVQLGQLIFLQFILVEELLDFLNLIGSIVQQVQFFDFLDSV
                     LAFVILSAVQCLIEQLGVFLSDDHPRKSHLIQLLVQFIQSINQQKVVEGGLFFELDVI
                     LLQIVQLSGFFPVIHVQALNPLKTPEVLRDDSDEVGILCMVAVEAPILDQDIVFHVIL
                     SFLSNQKVIAVD*"
     CDS             7131..7925
                     /label="ORF frame 3"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     gene            7134..7922
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     CDS             complement(7440..7976)
                     /label="ORF frame 1"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     terminator      8102..8221
                     /label="SV40_PA_terminator"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    8190..8209
                     /label="EBV_rev_primer"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     terminator      8412..8447
                     /label="T7_terminator"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    complement(8470..8487)
                     /label="Baculovirs_rev_primer"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    8477..8554
                     /label="3_AcPH"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     CDS             complement(8584..9201)
                     /label="ORF frame 3"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     misc_feature    9181..9214
                     /label="loxP"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     gene            complement(9982..10842)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     CDS             complement(9982..10842)
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
   61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
  121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
  181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
  241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
  301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
  361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
  421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
  481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
  541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
  601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
  661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
  721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
  781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
  841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
  901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
  961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc
 1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
 1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
 1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
 1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
 1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
 1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
 1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
 1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
 1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
 1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac
 1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac
 1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac
 1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg
 1801 agatatacca ccggtgccac catgtaccca tacgatgttc cagattacgc ttcgccgaag
 1861 aaaaagcgca aggtcgaagc gtccatgtac ttctataaaa ataaggaaaa caaactgaac
 1921 aaaaaggtgg tgctggggct ggatctggga atcgcttctg tcgggtggtg cctgaccgac
 1981 atctcccaga aagaggataa caagttccct atcattctgc acggagtgcg cctgtttgag
 2041 actgtcgacg attctgacga taagctgctg aatgaaaccc ggagaaagaa acgaggccag
 2101 aggcgccgaa accggagact gttcacaagg aagcgcgact ttatcaagta cctgatcgat
 2161 aacaatatca ttgagctgga gttcgacaaa aaccctaaga tcctggtgcg caacttcatc
 2221 gagaagtaca tcaacccatt ttcaaagaat ctggaactga agtacaaaag cgtcactaac
 2281 ctgccaatcg ggtttcacaa tctgcgcaaa gccgctatta acgagaagta caaactggat
 2341 aagagtgaac tgatcgtgct gctgtacttc tatctgtcac tgcggggagc attctttgac
 2401 aaccctgagg atacaaagtc aaaggaaatg aataagaacg agatcgaaat tttcgacaag
 2461 aacgagtcca tcaaaaatgc cgagttccca atcgataaga tcatcgagtt ttacaagatt
 2521 agcgggaaga tccggtccac tattaacctg aagtttggac atcaggacta cctgaaagag
 2581 atcaagcagg tgttcgaaaa gcagaacatc gatttcatga actacgagaa gttcgctatg
 2641 gaggaaaaat cattctttag ccgaatccgg aattacagcg aaggccccgg gaacgagaaa
 2701 agtttttcaa agtacgggct gtatgcaaat gaaaacggaa atcctgagct gatcattaat
 2761 gaaaagggcc agaagatcta tacaaagatt ttcaagactc tgtgggagtc aaaaatcggg
 2821 aagtgcagct acgacaagaa actgtataga gccccaaaga acagcttctc cgctaaagtg
 2881 tttgacatca caaataagct gactgattgg aagcacaaaa acgaatacat ctccgagcgc
 2941 ctgaagcgaa aaattctgct gtctaggttt ctgaacaaag attctaagag tgccgtggag
 3001 aaaatcctga aggaggaaaa cattaagttc gaaaatctgt ccgagatcgc atacaacaag
 3061 gacgataata agattaacct gccaatcatt aacgcctacc actctctgac cacaatcttc
 3121 aagaagcatc tgattaattt cgagaactat ctgatcagta acgaaaatga cctgtccaag
 3181 ctgatgtctt tctacaagca gcagagcgag aaactgtttg tgcccaatga gaagggctcc
 3241 tacgaaatca accagaacaa taacgtcctg catattttcg acgccatctc taacattctg
 3301 aacaagttta gtactatcca ggatagaatc aggattctgg aagggtattt cgagttttct
 3361 aacctgaaga aagacgtgaa aagctccgaa atctacagtg agattgctaa gctgcgggag
 3421 ttttccggca cctctagtct gtctttcggg gcatactata agttcatccc caatctgatt
 3481 tccgagggct ctaagaacta cagtacaatc tcatatgagg aaaaggccct gcagaatcag
 3541 aaaaataact tctcacacag caacctgttt gagaaaactt gggtggagga cctgatcgct
 3601 agtcctaccg tcaagcggtc actgagacag acaatgaatc tgctgaaaga gatcttcaag
 3661 tacagcgaga agaacaacct ggagatcgaa aagattgtgg tcgaggtgac caggtcaagc
 3721 aataacaaac atgagcgcaa gaaaatcgaa ggaattaaca agtaccgcaa ggagaaatat
 3781 gaggaactga agaaagtgta tgacctgccc aacgaaaata ctaccctgct gaagaaactg
 3841 tggctgctgc gacagcagca gggctacgac gcatattccc tgcggaaaat cgaggccaat
 3901 gatgtgatta acaagccatg gaattacgac atcgatcaca ttgtcccccg gtccatctct
 3961 ttcgacgata gtttttcaaa cctggtcatc gtcaacaagc tggacaatgc taagaaaagt
 4021 aacgatctgt cagcaaagca gttcatcgag aaaatctacg gcatcgagaa gctgaaagaa
 4081 gccaaggaga attgggggaa ctggtatctg cgcaacgcta atggaaaagc attcaacgac
 4141 aagggcaagt tcatcaagct gtataccatt gacaacctgg atgagttcga caatagcgat
 4201 tttatcaacc ggaatctgag cgatacctcc tacattacaa acgccctggt gaatcatctg
 4261 acttttagca attccaagta caagtactct gtggtcagtg tgaacggaaa gcagacctcc
 4321 aatctgagaa accagatcgc tttcgtcggc atcaagaata acaaggagac agaacgcgag
 4381 tggaagcgac ctgaggggtt caaatcaatc aacagcaatg actttctgat tagagaggaa
 4441 ggaaagaatg acgtgaaaga cgatgtcctg atcaaggata ggagcttcaa cggccaccat
 4501 gcagaggatg cctacttcat cactatcatc tctcagtact tcaggagttt taagaggatt
 4561 gagcgcctga acgtgaatta ccggaaagaa accagagagc tggacgatct ggagaagaat
 4621 aacatcaagt tcaaagaaaa ggcctccttc gacaactttc tgctgattaa tgctctggat
 4681 gaactgaacg agaagctgaa tcagatgcga ttttctcgga tggtcatcac taagaaaaac
 4741 acccagctgt tcaatgagac actgtacagc ggaaagtatg acaaaggcaa gaacactatc
 4801 aagaaagtcg aaaaactgaa cctgctggac aatagaaccg ataagattaa gaaaatcgag
 4861 gagttcttcg acgaggataa actgaaggaa aatgagctga ccaagctgca catcttcaac
 4921 catgataaga atctgtatga gacactgaaa atcatttgga acgaagtgaa gatcgagatc
 4981 aagaacaaga atctgaacga gaagaattac ttcaagtact tcgtgaacaa gaaactgcag
 5041 gaggggaaga tcagcttcaa tgaatgggtc cctattctgg acaacgattt caagatcatc
 5101 agaaagatca ggtacatcaa gttctcctct gaggaaaaag aaaccgacga gatcattttc
 5161 tctcagagta attttctgaa gatcgatcag agacagaact tctcctttca caatacactg
 5221 tactgggtgc agatttgggt ctataaaaac cagaaggacc agtactgttt catcagcatt
 5281 gatgcccgga acagcaaatt tgaaaaggac gagatcaaga ttaactacga gaagctgaaa
 5341 acccagaaag aaaagctgca gatcattaac gaggaaccaa ttctgaaaat caataagggc
 5401 gacctgttcg agaacgagga aaaggaactg ttttatatcg tggggaggga tgagaaaccc
 5461 cagaagctgg aaattaagta catcctggga aagaaaatca aagatcagaa gcagattcag
 5521 aagcccgtga agaaatactt ccctaattgg aagaaggtca atctgacata catgggcgag
 5581 atcttcaaga aataagaatt cggtaccgag ctcaagcttg cggccgccta tagtgtcacc
 5641 taaatcgtaa cccagctttc ttgtacaaag tggtcgggtg gcatccctgt gacccctccc
 5701 cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt gtcctaataa
 5761 aattaagttg catcattttg tctgactagg tgtccttcta taatattatg gggtggaggg
 5821 gggtggtatg gagcaagggg caagttggga tgacaacctg tagggcctgc ggggtctatt
 5881 gggaaccaag ctggagtgca gtggcacaat cttggctcac tgcaatctcc gcctcctggg
 5941 ttcaagcgat tctcctgcct cagcctcccg agttgttggg attccaggca tgcatgacca
 6001 ggctcagcta atttttgttt ttttggtaga gacggggttt caccatattg gccaggctgg
 6061 tctccaactc ctaatctcag gtgatctacc caccttggcc tcccaaattg ctgggattac
 6121 aggcgtgaac cactgctccc ttccctgtcc ttctagcata accccttggg gcctctaaac
 6181 gggtcttgag gggttttttg tctagaagac aatagcaggc atgctgggga tgcggtgggc
 6241 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
 6301 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
 6361 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
 6421 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
 6481 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
 6541 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
 6601 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
 6661 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
 6721 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
 6781 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
 6841 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
 6901 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
 6961 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
 7021 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
 7081 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
 7141 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
 7201 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
 7261 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
 7321 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
 7381 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
 7441 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
 7501 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
 7561 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
 7621 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
 7681 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
 7741 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
 7801 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
 7861 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
 7921 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
 7981 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
 8041 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
 8101 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
 8161 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
 8221 tcatgtctgt tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt
 8281 ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg
 8341 gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat
 8401 atgtaactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa
 8461 gcatgcggag gaaattctcc ttgaagtttc cctggtgttc aaagtaaagg agtttgcacc
 8521 agacgcacct ctgttcactg gtccggcgta ttaaaacacg atacattgtt attagtacat
 8581 ttattaagcg ctagattctg tgcgttgttg atttacagac aattgttgta cgtattttaa
 8641 taattcatta aatttataat ctttagggtg gtatgttaga gcgaaaatca aatgattttc
 8701 agcgtcttta tatctgaatt taaatattaa atcctcaata gatttgtaaa ataggtttcg
 8761 attagtttca aacaagggtt gtttttccga accgatggct ggactatcta atggattttc
 8821 gctcaacgcc acaaaacttg ccaaatcttg tagcagcaat ctagctttgt cgatattcgt
 8881 ttgtgttttg ttttgtaata aaggttcgac gtcgttcaaa atattatgcg cttttgtatt
 8941 tctttcatca ctgtcgttag tgtacaattg actcgacgta aacacgttaa atagagcttg
 9001 gacatattta acatcgggcg tgttagcttt attaggccga ttatcgtcgt cgtcccaacc
 9061 ctcgtcgtta gaagttgctt ccgaagacga ttttgccata gccacacgac gcctattaat
 9121 tgtgtcggct aacacgtccg cgatcaaatt tgtagttgag ctttttggaa ttgcgatcgc
 9181 ataacttcgt atagcataca ttatacgaag ttataagctc ggaacgctgc gctcggtcgt
 9241 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
 9301 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
 9361 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc ctgacgagca tcacaaaaat
 9421 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
 9481 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
 9541 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt
 9601 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
 9661 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
 9721 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
 9781 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
 9841 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
 9901 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
 9961 ggatctcaag aagatccttt gttaccaatg cttaatcagt gaggcaccta tctcagcgat
10021 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
10081 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
10141 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
10201 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
10261 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
10321 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
10381 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
10441 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
10501 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
10561 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
10621 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
10681 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
10741 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
10801 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
10861 ttattgaagc atttatcagg gttattgtct catgtccgcg cgtt
//

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