pEarleyGate201

价格:2000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: CAMBIA
载体名称: pEarleyGate201; pEarleyGate 201
质粒类型: Gateway载体;植物载体;农杆菌双元表达载体
高拷贝/低拷贝: 高拷贝
克隆方法: Gateway
启动子: 35S
载体大小: 11779 bp
5' 测序引物及序列: 35S promoter:CTATCCTTCGCAAGACCCTTC
3' 测序引物及序列: --
载体标签: HA (N ter)
载体抗性: 卡那霉素、氯霉素(仅空载体)
筛选标记: 草丁膦(磷丝菌素)phosphinothricin、
克隆菌株: DB3.1菌株
宿主细胞(系): 植物细胞、农杆菌
备注: --
产品目录号: --
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pEarleyGate201载体图谱



载体简介

载体序列

LOCUS       pEarleyGate 201        11779 bp ds-DNA     circular SYN 26-JUL-2016
DEFINITION  Gateway(R)-compatible plant transformation vector with an N-terminal
            HA tag.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11779)
  AUTHORS   Earley KW, Haag JR, Pontes O, Opper K, Juehne T, Song K, Pikaard CS.
  TITLE     Gateway-compatible vectors for plant functional genomics and 
            proteomics.
  JOURNAL   Plant J. 2006;45:616-29.
  PUBMED    16441352
REFERENCE   2  (bases 1 to 11779)
  AUTHORS   Pikaard Lab
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, July 26, 2016 from SnapGene Viewer 3.1.4 to Vector
            NTI(R) format
            http://www.snapgene.com
COMMENT     The sequence was corrected by inserting a G at position 7887.
COMMENT     VNTDATE|660009600|
COMMENT     VNTDBDATE|776390400|
COMMENT     VNTNAME|pEarleyGate 201|
COMMENT     VNTAUTHORNAME|Pikaard Lab|
FEATURES             Location/Qualifiers
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      111..363
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(373..924)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase草丁膦(磷丝菌素) 
                     乙酰转移酶"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
     promoter        complement(930..1310)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     promoter        2306..2651
                     /label=35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    2681..2735
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             2737..2739
                     /codon_start=1
                     /product="start codon"
                     /label=ATG
     CDS             2740..2766
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
     protein_bind    2770..2889
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2919..2949
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3003..3662
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
     CDS             4004..4309
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
     protein_bind    complement(4350..4474)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      4550..5257
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     misc_feature    5501..5525
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6825..7454
                     /codon_start=1
                     /product="stability protein from Pseudomonas plasmid pVS1"
                     /label=pVS1 StaA
     CDS             7883..8956
                     /codon_start=1
                     /product="replication protein from Pseudomonas plasmid 
                     pVS1"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9022..9216
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    9560..9700
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9886..10474)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10561..11355)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tccaatccca caaaaatctg agcttaacag cacagttgct cctctcagag cagaatcggg
     1381 tattcaacac cctcatatca actactacgt tgtgtataac ggtccacatg ccggtatata
     1441 cgatgactgg ggttgtacaa aggcggcaac aaacggcgtt cccggagttg cacacaagaa
     1501 atttgccact attacagagg caagagcagc agctgacgcg tacacaacaa gtcagcaaac
     1561 agacaggttg aacttcatcc ccaaaggaga agctcaactc aagcccaaga gctttgctaa
     1621 ggccctaaca agcccaccaa agcaaaaagc ccactggctc acgctaggaa ccaaaaggcc
     1681 cagcagtgat ccagccccaa aagagatctc ctttgccccg gagattacaa tggacgattt
     1741 cctctatctt tacgatctag gaaggaagtt cgaaggtgaa ggtgacgaca ctatgttcac
     1801 cactgataat gagaaggtta gcctcttcaa tttcagaaag aatgctgacc cacagatggt
     1861 tagagaggcc tacgcagcag gtctcatcaa gacgatctac ccgagtaaca atctccagga
     1921 gatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga ctaattgcat
     1981 caagaacaca gagaaagaca tatttctcaa gatcagaagt actattccag tatggacgat
     2041 tcaaggcttg cttcataaac caaggcaagt aatagagatt ggagtctcta aaaaggtagt
     2101 tcctactgaa tctaaggcca tgcatggagt ctaagattca aatcgaggat ctaacagaac
     2161 tcgccgtgaa gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga
     2221 aaatcttcgt caacatggtg gagcacgaca ctctggtcta ctccaaaaat gtcaaagata
     2281 cagtctcaga agaccaaagg gctattgaga cttttcaaca aaggataatt tcgggaaacc
     2341 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag
     2401 gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa gatctctctg
     2461 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
     2521 ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac gtaagggatg
     2581 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
     2641 tggagaggac acgctcgagt ataagagctc tatttttaca acaattacca acaacaacaa
     2701 acaacaaaca acattacaat tacatttaca attaccatgt acccatacga tgttccagat
     2761 tacgctatca caagtttgta caaaaaagct gaacgagaaa cgtaaaatga tataaatatc
     2821 aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat
     2881 atccagtcat attggcggcc gcattaggca ccccaggctt tacactttat gcttccggct
     2941 cgtataatgt gtggattttg agttaggatc cgtcgagatt ttcaggagct aaggaagcta
     3001 aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag
     3061 aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg
     3121 atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta
     3181 ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg
     3241 gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg
     3301 aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat
     3361 attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg
     3421 agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg
     3481 tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag
     3541 gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc
     3601 atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt
     3661 aaacgcgtgg atccggctta ctaaaagcca gataacagta tgcgtatttg cgcgctgatt
     3721 tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa agaggtatgc
     3781 tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc tcaaggcata
     3841 tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg
     3901 cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt
     3961 gaaatgaacg gctcttttgc tgacgagaac aggggctggt gaaatgcagt ttaaggttta
     4021 cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga
     4081 cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt
     4141 ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac
     4201 cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc tcagccaccg
     4261 cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggctc
     4321 ccttatacac agccagtctg caggtcgacc atagtgactg gatatgttgt gttttacagc
     4381 attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt
     4441 acgtttctcg ttcagctttc ttgtacaaag tggtgcctag gtgagtctag agagttaatc
     4501 taggtgagtc tagagagtta attaagaccc gggactagtc cctagagtcc tgctttaatg
     4561 agatatgcga gacgcctatg atcgcatgat atttgctttc aattctgttg tgcacgttgt
     4621 aaaaaacctg agcatgtgta gctcagatcc ttaccgccgg tttcggttca ttctaatgaa
     4681 tatatcaccc gttactatcg tatttttatg aataatattc tccgttcaat ttactgattg
     4741 taccctacta cttatatgta caatattaaa atgaaaacaa tatattgtgc tgaataggtt
     4801 tatagcgaca tctatgatag agcgccacaa taacaaacaa ttgcgtttta ttattacaaa
     4861 tccaatttta aaaaaagcgg cagaaccggt caaacctaaa agactgatta cataaatctt
     4921 attcaaattt caaaagtgcc ccaggggcta gtatctacga cacaccgagc ggcgaactaa
     4981 taacgctcac tgaagggaac tccggttccc cgccggcgcg catgggtgag attccttgaa
     5041 gttgagtatt ggccgtccgc tctaccgaaa gttacgggca ccattcaacc cggtccagca
     5101 cggcggccgg gtaaccgact tgctgccccg agaattatgc agcatttttt tggtgtatgt
     5161 gggccccaaa tgaagtgcag gtcaaacctt gacagtgacg acaaatcgtt gggcgggtcc
     5221 agggcgaatt ttgcgacaac atgtcgaggc tcagcaggac ctgcaggcat gcaagcttgg
     5281 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     5341 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     5401 gcccttccca acagttgcgc agcctgaatg gcgaatgcta gagcagcttg agcttggatc
     5461 agattgtcgt ttcccgcctt cagtttaaac tatcagtgtt tgacaggata tattggcggg
     5521 taaacctaag agaaaagagc gtttattaga ataacggata tttaaaaggg cgtgaaaagg
     5581 tttatccgtt cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggatcaaag
     5641 tactttgatc caacccctcc gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc
     5701 gtcttctgaa aacgacatgt cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc
     5761 cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata aagtagaata cttgcgacta
     5821 gaaccggaga cattacgcca tgaacaagag cgccgccgct ggcctgctgg gctatgcccg
     5881 cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc gaactgcacg cggccggctg
     5941 caccaagctg ttttccgaga agatcaccgg caccaggcgc gaccgcccgg agctggccag
     6001 gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg accaggctag accgcctggc
     6061 ccgcagcacc cgcgacctac tggacattgc cgagcgcatc caggaggccg gcgcgggcct
     6121 gcgtagcctg gcagagccgt gggccgacac caccacgccg gccggccgca tggtgttgac
     6181 cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc atcgaccgca cccggagcgg
     6241 gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc
     6301 acagatcgcg cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga aagaggcggc
     6361 tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt
     6421 gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag gacgcattga ccgaggccga
     6481 cgccctggcg gccgccgaga atgaacgcca agaggaacaa gcatgaaacc gcaccaggac
     6541 ggccaggacg aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcggccggg
     6601 tacgtgttcg agccgcccgc gcacgtctca accgtgcggc tgcatgaaat cctggccggt
     6661 ttgtctgatg ccaagctggc ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc
     6721 cgccgtctaa aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg
     6781 tatatgatgc gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg
     6841 tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc
     6901 tgcaactcgc cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg
     6961 attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga
     7021 ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc
     7081 aggcggcgga cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc
     7141 agccaagccc ttacgacata tgggccaccg ccgacctggt ggagctggtt aagcagcgca
     7201 ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca
     7261 cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta cgagctgccc attcttgagt
     7321 cccgtatcac gcagcgcgtg agctacccag gcactgccgc cgccggcaca accgttcttg
     7381 aatcagaacc cgagggcgac gctgcccgcg aggtccaggc gctggccgct gaaattaaat
     7441 caaaactcat ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag
     7501 tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc tggcagacac
     7561 gccagccatg aagcgggtca actttcagtt gccggcggag gatcacacca agctgaagat
     7621 gtacgcggta cgccaaggca agaccattac cgagctgcta tctgaataca tcgcgcagct
     7681 accagagtaa atgagcaaat gaataaatga gtagatgaat tttagcggct aaaggaggcg
     7741 gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga atgccccatg tgtggaggaa
     7801 cgggcggttg gccaggcgta agcggctggg ttgtctgccg gccctgcaat ggcactggaa
     7861 cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg
     7921 cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca
     7981 acgcatcgag gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg
     8041 caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg
     8101 cgacgagcaa ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg
     8161 cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt
     8221 gatccgctac gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc
     8281 cagtgtgtgg gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa
     8341 ccgataccgg gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga
     8401 cgtactcaag ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac
     8461 ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg
     8521 ccgcctggtg acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag
     8581 cgaaaccggg cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat
     8641 cacagaaggc aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc
     8701 cggcatcggc cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag
     8761 atggttgttc aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg
     8821 tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga
     8881 ggcggggcag gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc
     8941 atccgccggt tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa
     9001 aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat
     9061 tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat
     9121 gtaagtgact gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact
     9181 tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga
     9241 agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg
     9301 tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc
     9361 agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct
     9421 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     9481 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     9541 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata
     9601 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
     9661 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct
     9721 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     9781 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     9841 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     9901 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     9961 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
    10021 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
    10081 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
    10141 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
    10201 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
    10261 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
    10321 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
    10381 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
    10441 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
    10501 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta
    10561 ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc
    10621 cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg
    10681 gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa
    10741 gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa
    10801 gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt
    10861 aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa
    10921 gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc
    10981 gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg
    11041 ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct
    11101 ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca
    11161 tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt
    11221 catttttaaa tataggtttt cattttctcc caccagctta tataccttag caggagacat
    11281 tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat
    11341 tctcatttta gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa
    11401 gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa
    11461 taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg
    11521 gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa
    11581 catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc
    11641 cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc
    11701 cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg
    11761 ggagctgttg gctggctgg
//

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