| 质粒名称: | pYW473 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002591 |
| 产品用途: | AAV packaging plasmid expressing rep-AAV-PHP.eB cap (modified from Addgene plasmid no. 103005). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS |
| 表达系统: | 腺病毒 |
| 启动子: | CMV |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | PHP.eB |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
pYW473
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pYW473载体质粒基本信息
pYW473质粒图谱载体图谱和pYW473载体序列质粒序列多克隆位点信息
pYW473质粒载体简介
pYW473质粒序列载体序列
LOCUS pYW473 7351 bp DNA circular SYN 18-APR-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM recombinant plasmid
REFERENCE 1 (bases 1 to 7351)
AUTHORS Macapp
TITLE Direct Submission
JOURNAL Exported Jun 22, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7351
/mol_type="other DNA"
/organism="recombinant plasmid"
primer_bind 6..27
/label=PMD-F
primer_bind 45..63
/label=1300-R
primer_bind 79..99
/label=PMDC83-F
primer_bind 86..105
/label=PMAL-C2X-R
primer_bind 91..108
/label=2.0rev
primer_bind 93..112
/label=P2?(R)A374)
primer_bind 110..133
/label=M13F(-47)
primer_bind 111..127
/label=pSOS-3'
primer_bind 117..136
/label=pGreenII-LUC-F
primer_bind 119..137
/label=pHis2.1-F
primer_bind 136..153
/label=M13F
primer_bind 136..153
/label=Seq-F
primer_bind 137..153
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 163..181
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 163..181
/label=T7
primer_bind 163..180
/label=T72G
primer_bind 211..227
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 1073..1091
/label=oYW1050
primer_bind 1555..1570
/label=oYW1030
/note="AAV9n seq primer"
primer_bind 1823..1840
/label=oYW1036
/note="LA(HindIII)-AAV9n"
primer_bind complement(2010..2030)
/label=oYW1067
/note="PHP.eB seq primer"
CDS 2164..3922
/codon_start=1
/label=Rep/Cap
/translation="MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQHQDNARGL
VLPGYKYLGPGNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLKYNHADAEFQERL
KEDTSFGGNLGRAVFQAKKRLLEPLGLVEEAAKTAPGKKRPVEQSPQEPDSSAGIGKSG
AQPAKKRLNFGQTGDTESVPDPQPIGEPPAAPSGVGSLTMASGGGAPVADNNEGADGVG
SSSGNWHCDSQWLGDRVITTSTRTWALPTYNNHLYKQISNSTSGGSSNDNAYFGYSTPW
GYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTDNNGVKTIANNLTS
TVQVFTDSDYQLPYVLGSAHEGCLPPFPADVFMIPQYGYLTLNDGSQAVGRSSFYCLEY
FPSQMLRTGNNFQFSYEFENVPFHSSYAHSQSLDRLMNPLIDQYLYYLSKTINGSGQNQ
QTLKFSVAGPSNMAVQGRNYIPGPSYRQQRVSTTVTQNNNSEFAWPGASSWALNGRNSL
MNPGPAMASHKEGEDRFFPLSGSLIFGKQGTGRDNVDADKVMITNEEEIKTTNPVATES
YGQVATNHQS"
primer_bind 2727..2743
/label=oYW1051
primer_bind complement(3638..3661)
/label=oYW1031
/note="AAV9n seq primer"
misc_feature 3899..3921
/label=特征 14
primer_bind complement(3903..3948)
/label=oYW1037
/note="AAV9n-PHP.eB-DGTLAVPFK-1"
primer_bind 3923..3964
/label=oYW1038
/note="AAV9n-PHP.eB-DGTLAVPFK-2"
CDS 3949..4395
/codon_start=1
/label=Rep/Cap
/translation="AQAQTGWVQNQGILPGMVWQDRDVYLQGPIWAKIPHTDGNFHPSP
LMGGFGMKHPPPQILIKNTPVPADPPTAFNKDKLNSFITQYSTGQVSVEIEWELQKENS
KRWNPEIQYTSNYYKSNNVEFAVNTEGVYSEPRPIGTRYLTRNL"
primer_bind complement(4472..4495)
/label=oYW1039
/note="RA(SbfI)-AAV9n"
primer_bind complement(4698..4717)
/label=T3
promoter complement(4700..4718)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(4738..4755)
/label=M13R
primer_bind complement(4738..4755)
/label=Seq-R
primer_bind complement(4739..4755)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4751..4774)
/label=M13R(-48)
primer_bind complement(4752..4771)
/label=PBI101-F
primer_bind complement(4752..4771)
/label=pKNTG.1F
primer_bind complement(4753..4776)
/label=pQLink-F
primer_bind complement(4753..4774)
/label=PQE30-F
protein_bind 4763..4779
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind complement(4765..4782)
/label=PKNT2F
promoter complement(4787..4817)
/label=lac promoter
/note="promoter for the E. coli lac operon"
primer_bind complement(4795..4814)
/label=PC1300-F
primer_bind complement(4804..4823)
/label=P1?(R)A373)
primer_bind complement(4804..4823)
/label=PBI101-F (1)
primer_bind complement(4825..4846)
/label=1300-F
primer_bind complement(4827..4847)
/label=3.0rev
protein_bind 4832..4853
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4904..4923)
/label=PMD-R
primer_bind complement(4970..4987)
/label=L4440
primer_bind complement(5019..5042)
/label=PTRE-R?(R)A3254?(C)
primer_bind complement(5096..5115)
/label=oYW207
/note="pLL3.7 PCR R"
primer_bind complement(5126..5139)
/label=oYW49
/note="ColE1 origin R for PBS-C/D box elongation"
rep_origin complement(5141..5729)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5221..5240)
/label=PBR322ori-F
primer_bind 5221..5240
/label=PBR322ori-R
primer_bind complement(5280..5299)
/label=W5CF0703-P099-D09
/note="Sequencing primer"
primer_bind 5779..5802
/label=BlaRV
CDS complement(5900..6760)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(5917..5936)
/label=W4CF0701-P099-E08
/note="Sequencing primer"
primer_bind complement(5984..6007)
/label=BlaM1
primer_bind 6128..6147
/label=anbian-R
primer_bind complement(6320..6339)
/label=anbian-F
primer_bind complement(6581..6600)
/label=W3CF0630-P099-D08
/note="Sequencing primer"
primer_bind complement(6637..6656)
/label=W3CF0630-P099-A08
/note="Sequencing primer"
primer_bind complement(6637..6656)
/label=W3CF0630-P099-F07
/note="Sequencing primer"
primer_bind 6731..6750
/label=oYW24
/note="Forward seq primer of AmpR"
promoter complement(6761..6865)
/gene="bla"
/label=AmpR promoter
primer_bind 6796..6817
/label=163-F
rep_origin complement(6891..7346)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6967..6986)
/label=W3F0630-P099-E08
/note="Sequencing primer"
primer_bind complement(7028..7049)
/label=F1ori-F
primer_bind 7086..7106
/label=oYW498
/note="f1 ori PCR f"
primer_bind complement(7089..7103)
/label=oYW1030
/note="AAV9n seq primer"
primer_bind complement(7185..7203)
/label=W3F0630-P099-F08
/note="Sequencing primer"
primer_bind 7240..7259
/label=F1ori-R
ORIGIN
1 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
61 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
121 tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag
181 ggcgaattgg gtaccgggcc ccccctcgat cgaggtcgac ggtatcgggg gagctcgcag
241 ggtctccatt ttgaagcggg aggtttgaac gcgcagccgc catgccgggg ttttacgaga
301 ttgtgattaa ggtccccagc gaccttgacg agcatctgcc cggcatttct gacagctttg
361 tgaactgggt ggccgagaag gaatgggagt tgccgccaga ttctgacatg gatctgaatc
421 tgattgagca ggcacccctg accgtggccg agaagctgca gcgcgacttt ctgacggaat
481 ggcgccgtgt gagtaaggcc ccggaggctc ttttctttgt gcaatttgag aagggagaga
541 gctacttcca catgcacgtg ctcgtggaaa ccaccggggt gaaatccatg gttttgggac
601 gtttcctgag tcagattcgc gaaaaactga ttcagagaat ttaccgcggg atcgagccga
661 ctttgccaaa ctggttcgcg gtcacaaaga ccagaaatgg cgccggaggc gggaacaagg
721 tggtggatga gtgctacatc cccaattact tgctccccaa aacccagcct gagctccagt
781 gggcgtggac taatatggaa cagtatttaa gcgcctgttt gaatctcacg gagcgtaaac
841 ggttggtggc gcagcatctg acgcacgtgt cgcagacgca ggagcagaac aaagagaatc
901 agaatcccaa ttctgatgcg ccggtgatca gatcaaaaac ttcagccagg tacatggagc
961 tggtcgggtg gctcgtggac aaggggatta cctcggagaa gcagtggatc caggaggacc
1021 aggcctcata catctccttc aatgcggcct ccaactcgcg gtcccaaatc aaggctgcct
1081 tggacaatgc gggaaagatt atgagcctga ctaaaaccgc ccccgactac ctggtgggcc
1141 agcagcccgt ggaggacatt tccagcaatc ggatttataa aattttggaa ctaaacgggt
1201 acgatcccca atatgcggct tccgtctttc tgggatgggc cacgaaaaag ttcggcaaga
1261 ggaacaccat ctggctgttt gggcctgcaa ctaccgggaa gaccaacatc gcggaggcca
1321 tagcccacac tgtgcccttc tacgggtgcg taaactggac caatgagaac tttcccttca
1381 acgactgtgt cgacaagatg gtgatctggt gggaggaggg gaagatgacc gccaaggtcg
1441 tggagtcggc caaagccatt ctcggaggaa gcaaggtgcg cgtggaccag aaatgcaagt
1501 cctcggccca gatagacccg actcccgtga tcgtcacctc caacaccaac atgtgcgccg
1561 tgattgacgg gaactcaacg accttcgaac accagcagcc gttgcaagac cggatgttca
1621 aatttgaact cacccgccgt ctggatcatg actttgggaa ggtcaccaag caggaagtca
1681 aagacttttt ccggtgggca aaggatcacg tggttgaggt ggagcatgaa ttctacgtca
1741 aaaagggtgg agccaagaaa agacccgccc ccagtgacgc agatataagt gagcccaaac
1801 gggtgcgcga gtcagttgcg cagccatcga cgtcagacgc ggaagcttcg atcaactacg
1861 cagacaggta ccaaaacaaa tgttctcgtc acgtgggcat gaatctgatg ctgtttccct
1921 gcagacaatg cgagagaatg aatcagaatt caaatatctg cttcactcac ggacagaaag
1981 actgtttaga gtgctttccc gtgtcagaat ctcaacccgt ttctgtcgtc aaaaaggcgt
2041 atcagaaact gtgctacatt catcatatca tgggaaaggt gccagacgct tgcactgcct
2101 gcgatctggt caatgtggat ttggatgact gcatctttga acaataaatg atttaaatca
2161 ggtatggctg ccgatggtta tcttccagat tggctcgagg acaaccttag tgaaggaatt
2221 cgcgagtggt gggctttgaa acctggagcc cctcaaccca aggcaaatca acaacatcaa
2281 gacaacgctc gaggtcttgt gcttccgggt tacaaatacc ttggacccgg caacggactc
2341 gacaaggggg agccggtcaa cgcagcagac gcggcggccc tcgagcacga caaggcctac
2401 gaccagcagc tcaaggccgg agacaacccg tacctcaagt acaaccacgc cgacgccgag
2461 ttccaggagc ggctcaaaga agatacgtct tttgggggca acctcgggcg agcagtcttc
2521 caggccaaaa agaggcttct tgaacctctt ggtctggttg aggaagcggc taagacggct
2581 cctggaaaga agaggcctgt agagcagtct cctcaggaac cggactcctc cgcgggtatt
2641 ggcaaatcgg gtgcacagcc cgctaaaaag agactcaatt tcggtcagac tggcgacaca
2701 gagtcagtcc cagaccctca accaatcgga gaacctcccg cagccccctc aggtgtggga
2761 tctcttacaa tggcttcagg tggtggcgca ccagtggcag acaataacga aggtgccgat
2821 ggagtgggta gttcctcggg aaattggcat tgcgattccc aatggctggg ggacagagtc
2881 atcaccacca gcacccgaac ctgggccctg cccacctaca acaatcacct ctacaagcaa
2941 atctccaaca gcacatctgg aggatcttca aatgacaacg cctacttcgg ctacagcacc
3001 ccctgggggt attttgactt caacagattc cactgccact tctcaccacg tgactggcag
3061 cgactcatca acaacaactg gggattccgg cctaagcgac tcaacttcaa gctcttcaac
3121 attcaggtca aagaggttac ggacaacaat ggagtcaaga ccatcgccaa taaccttacc
3181 agcacggtcc aggtcttcac ggactcagac tatcagctcc cgtacgtgct cgggtcggct
3241 cacgagggct gcctcccgcc gttcccagcg gacgttttca tgattcctca gtacgggtat
3301 ctgacgctta atgatggaag ccaggccgtg ggtcgttcgt ccttttactg cctggaatat
3361 ttcccgtcgc aaatgctaag aacgggtaac aacttccagt tcagctacga gtttgagaac
3421 gtacctttcc atagcagcta cgctcacagc caaagcctgg accgactaat gaatccactc
3481 atcgaccaat acttgtacta tctctcaaag actattaacg gttctggaca gaatcaacaa
3541 acgctaaaat tcagtgtggc cggacccagc aacatggctg tccagggaag aaactacata
3601 cctggaccca gctaccgaca acaacgtgtc tcaaccactg tgactcaaaa caacaacagc
3661 gaatttgctt ggcctggagc ttcttcttgg gctctcaatg gacgtaatag cttgatgaat
3721 cctggacctg ctatggccag ccacaaagaa ggagaggacc gtttctttcc tttgtctgga
3781 tctttaattt ttggcaaaca aggaactgga agagacaacg tggatgcgga caaagtcatg
3841 ataaccaacg aagaagaaat taaaactact aacccggtag caacggagtc ctatggacaa
3901 gtggccacaa accaccagag tgatgggact ttggcggtgc cttttaaggc acaggcgcag
3961 accggctggg ttcaaaacca aggaatactt ccgggtatgg tttggcagga cagagatgtg
4021 tacctgcaag gacccatttg ggccaaaatt cctcacacgg acggcaactt tcacccttct
4081 ccgctgatgg gagggtttgg aatgaagcac ccgcctcctc agatcctcat caaaaacaca
4141 cctgtacctg cggatcctcc aacggccttc aacaaggaca agctgaactc tttcatcacc
4201 cagtattcta ctggccaagt cagcgtggag atcgagtggg agctgcagaa ggaaaacagc
4261 aagcgctgga acccggagat ccagtacact tccaactatt acaagtctaa taatgttgaa
4321 tttgctgtta atactgaagg tgtatatagt gaaccccgcc ccattggcac cagatacctg
4381 actcgtaatc tgtaattgct tgttaatcaa taaaccgttt aattcgtttc agttgaactt
4441 tggtctctgc gaagggcgaa ttcgtttaaa cctgcaggac tagaggtcct gtattagagg
4501 tcacgtgagt gttttgcgac attttgcgac accatgtggt cacgctgggt atttaagccc
4561 gagtgagcac gcagggtctc cattttgaag cgggaggttt gaacgcgcag ccgccaagcc
4621 gaattctgca gatatccatc acactggcgg ccgctcgact agagcggccg ccaccgcggt
4681 ggagctccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt
4741 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
4801 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
4861 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
4921 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
4981 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
5041 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
5101 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
5161 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
5221 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
5281 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
5341 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
5401 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
5461 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
5521 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
5581 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
5641 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
5701 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
5761 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
5821 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
5881 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
5941 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
6001 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
6061 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
6121 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
6181 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
6241 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
6301 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
6361 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
6421 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
6481 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
6541 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
6601 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
6661 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
6721 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
6781 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
6841 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag
6901 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
6961 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
7021 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
7081 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
7141 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
7201 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
7261 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
7321 gcttaatgcg ccgctacagg gcgcgtccca t
//
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pYW473质粒载体应用举例
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