| 质粒名称: | pSLQ5079_pHR_PGK_sfGFP_CoV-F1 |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 155303 |
| 存储实验室: | Stanley Qi |
|
载体骨架基本信息 |
|
| 载体名称: | pHR |
| 空载体大小: | 8965 bp |
| 完整质粒大小: | 11685bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Superfolder GFP fused with SARS-CoV-F1/SFGFP/SARS-CoV-F1 |
| 物种来源: | Synthetic |
| 插入大小: | 2717 bp |
| 启动子: | PGK |
| 标签/融合蛋白: | 人细胞色素c氧化酶亚基VIII（N端） |
| 5’测序引物 | CATTCTGCACGCTTCAAAAG |
| 3’测序引物 | CATAGCGTAAAAGGAGCAACA |
|
文献引用方法 |
|
| 材料和方法部分: |
pSLQ5079_pHR_PGK_sfGFP_CoV-F1 was a gift from Stanley Qi (Addgene plasmid # 155303 ; http://n2t.net/addgene:155303 ; RRID:Addgene_155303)
|
| 参考文献部分: | Development of CRISPR as an Antiviral Strategy to Combat SARS-CoV-2 and Influenza. Abbott TR, Dhamdhere G, Liu Y, Lin X, Goudy L, Zeng L, Chemparathy A, Chmura S, Heaton NS, Debs R, Pande T, Endy D, La Russa MF, Lewis DB, Qi LS. Cell. 2020 May 14;181(4):865-876.e12. doi: 10.1016/j.cell.2020.04.020. Epub 2020 Apr 29. 10.1016/j.cell.2020.04.020 PubMed 32353252 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pSLQ5079_pHR_PGK_sfGFP_CoV-F1 11685 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION SARS-CoV-2 fluorescent reporter 1.
ACCESSION .
VERSION .
KEYWORDS pSLQ5079_pHR_PGK_sfGFP_CoV-F1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11685)
AUTHORS Abbott TR, Dhamdhere G, Liu Y, Lin X, Goudy L, Zeng L, Chemparathy
A, Chmura S, Heaton NS, Debs R, Pande T, Endy D, La Russa MF, Lewis
DB, Qi LS
TITLE Development of CRISPR as an Antiviral Strategy to Combat SARS-CoV-2
and Influenza.
JOURNAL Cell. 2020 May 14;181(4):865-876.e12. doi:
10.1016/j.cell.2020.04.020. Epub 2020 Apr 29.
PUBMED 32353252
REFERENCE 2 (bases 1 to 11685)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..11685
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(1..17)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2..18)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 108..341
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
promoter complement(366..384)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(367..384)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(674..693)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind complement(907..926)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 1026..1048
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(1086..1104)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 1172..1276
/gene="bla"
/label=AmpR promoter
CDS 1277..2137
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(1495..1514)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 2308..2896
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 2797..2816
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 3050..3067
/label=L4440
/note="L4440 vector, forward primer"
primer_bind complement(3137..3157)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 3142..3471
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3322..3457
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3384..3403
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
intron 4605..4670
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 4800..4820
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 5245..5379
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5282..5301)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5336..5355
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
LTR 5547..6180
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 6227..6352
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 6849..7082
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 7267..7311
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 7596..7713
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 7791..8290
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(7826..7849)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 8223..8242
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
CDS 8347..9060
/codon_start=1
/product="GFP variant that folds robustly even when fused
to poorly folded proteins (Pedelacq et al., 2006)"
/label=superfolder GFP
/translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
FVTAAGITHGMDELYK"
primer_bind complement(8377..8405)
/label=GFP-R
/note="GFP, reverse primer. Does NOT anneal to EGFP"
primer_bind 9000..9020
/label=GFP-F
/note="GFP, forward primer. Does NOT anneal to EGFP"
misc_feature 11095..11683
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(11148..11168)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(11566..11577)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
ORIGIN
1 cgataccgtc gacctcgagg gaattaattc gagctcggta cctttaagac caatgactta
61 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
121 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
181 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
241 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
301 atccctcaga cccttttagt cagtgtggaa aatctctagc agcatctaga attaattccg
361 tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg
421 tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag
481 cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc
541 tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat cagcagggtc
601 atcgctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
661 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
721 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
781 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
841 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg aatggtgcac
901 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
961 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
1021 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
1081 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
1141 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
1201 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
1261 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
1321 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
1381 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
1441 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
1501 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
1561 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
1621 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
1681 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
1741 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
1801 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
1861 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
1921 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
1981 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
2041 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
2101 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
2161 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
2221 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
2281 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
2341 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
2401 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
2461 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
2521 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
2581 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
2641 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
2701 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
2761 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
2821 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
2881 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
2941 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
3001 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
3061 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gtcggccgat
3121 tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
3181 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
3241 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
3301 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
3361 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
3421 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttgga
3481 cacaagacag gcttgcgaga tatgtttgag aataccactt tatcccgcgt cagggagagg
3541 cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta
3601 taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg
3661 gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg
3721 taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg
3781 tggcggtctg taccgggtgc gttactggcg cgtgaactgg gtattcgtca tgtcgatacc
3841 gtttgtattt ccagctacga tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca
3901 gaaggcgatg gcgaaggctt catcgttatt gatgacctgg tggataccgg tggtactgcg
3961 gttgcgattc gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc aaaaccggct
4021 ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc aagatacctg gattgaacag
4081 ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc
4141 ctggcactgc cgggcgttgt tctttttaac ttcaggcggg ttacaatagt ttccagtaag
4201 tattctggag gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg
4261 ctttaaacat cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc
4321 tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct
4381 gatgtggatc tggcgcggca ttgacccacg cgaaatcctc gacgtccagg cacgtattgt
4441 gatgagcgat gccgaacgta ccgacgatga tttatacgat acggtgattg gctaccgtgg
4501 cggcaactgg atttatgagt gggccccgga tctttgtgaa ggaaccttac ttctgtggtg
4561 tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt
4621 taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct
4681 atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct
4741 cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc
4801 caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt
4861 tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg
4921 aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta
4981 ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt
5041 ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg
5101 ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca
5161 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
5221 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
5281 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
5341 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac
5401 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt
5461 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt
5521 taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga
5581 tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc agaactacac
5641 accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
5701 tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt
5761 gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg
5821 cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata
5881 tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg
5941 gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg
6001 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
6061 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
6121 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
6181 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
6241 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
6301 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
6361 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
6421 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
6481 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
6541 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
6601 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
6661 aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga
6721 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
6781 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
6841 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
6901 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
6961 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
7021 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
7081 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
7141 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
7201 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
7261 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
7321 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
7381 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
7441 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
7501 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct
7561 cgacggtatc gccaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
7621 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
7681 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
7741 ccagtttgga tcgataagct tgatatctaa ttcctgcagc caattctacc gggtagggga
7801 ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg
7861 ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc
7921 tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc
7981 ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag
8041 tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg
8101 ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg
8161 gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc
8221 ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc
8281 gggcctttcg acctgcagcc caagcttggg gatctggagc tctcgagaat tctcacgcgt
8341 gccaccatga gcaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta
8401 gatggtgatg ttaatgggca caaattttct gtccgtggag agggtgaagg tgatgctaca
8461 aacggaaaac tcacccttaa atttatttgc actactggaa aactacctgt tccgtggcca
8521 acacttgtca ctactctgac ctatggtgtt caatgctttt cccgttatcc ggatcacatg
8581 aaacggcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga acgcactata
8641 tctttcaaag atgacgggac ctacaagacg cgtgctgaag tcaagtttga aggtgatacc
8701 cttgttaatc gtatcgagtt aaagggtatt gattttaaag aagatggaaa cattcttgga
8761 cacaaactcg agtacaactt taactcacac aatgtataca tcacggcaga caaacaaaag
8821 aatggaatca aagctaactt caaaattcgc cacaacgttg aagatggttc cgttcaacta
8881 gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac
8941 cattacctgt cgacacaatc tgtcctttcg aaagatccca acgaaaagcg tgaccacatg
9001 gtccttcttg agtttgtaac tgctgctggg attacacatg gcatggatga gctctacaaa
9061 taacttcagt cagctgatgc acaatcgttt ttaaacgggt ttgcggtgta agtgcagccc
9121 gtcttacacc gtgcggcaca ggcactagta ctgatgtcgt atacagggct tttgacatct
9181 acaatgataa agtagctggt tttgctaaat tcctaaaaac taattgttgt cgcttccaag
9241 aaaaggacga agatgacaat ttaattgatt cttactttgt agttaagaga cacactttct
9301 ctaactacca acatgaagaa acaatttata atttacttaa ggattgtcca gctgttgcta
9361 aacatgactt ctttaagttt agaatagacg gtgacatggt accacatata tcacgtcaac
9421 gtcttactaa atacacaatg gcagacctcg tctatgcttt aaggcatttt gatgaaggta
9481 attgtgacac attaaaagaa atacttgtca catacaattg ttgtgatgat gattatttca
9541 ataaaaagga ctggtatgat tttgtagaaa acccagatat attacgcgta tacgccaact
9601 taggtgaacg tgtacgccaa gctttgttaa aaacagtaca attctgtgat gccatgcgaa
9661 atgctggtat tgttggtgta ctgacattag ataatcaaga tctcaatggt aactggtatg
9721 atttcggtga tttcatacaa accacgccag gtagtggagt tcctgttgta gattcttatt
9781 attcattgtt aatgcctata ttaaccttga ccagggcttt aactgcagag tcacatgttg
9841 acactgactt aacaaagcct tacattaagt gggatttgtt aaaatatgac ttcacggaag
9901 agaggttaaa actctttgac cgttatttta aatattggga tcagacatac cacccaaatt
9961 gtgttaactg tttggatgac agatgcattc tgcattgtgc aaactttaat gttttattct
10021 ctacagtgtt cccacctaca agttttggac cactagtgag aaaaatattt gttgatggtg
10081 ttccatttgt agtttcaact ggataccact tcagagagct aggtgttgta cataatcagg
10141 atgtaaactt acatagctct agacttagtt ttaaggaatt acttgtgtat gctgctgacc
10201 ctgctatgca cgctgcttct ggtaatctat tactagataa acgcactacg tgcttttcag
10261 tagctgcact tactaacaat gttgcttttc aaactgtcaa acccggtaat tttaacaaag
10321 acttctatga ctttgctgtg tctaagggtt tctttaagga aggaagttct gttgaattaa
10381 aacacttctt ctttgctcag gatggtaatg ctgctatcag cgattatgac tactatcgtt
10441 ataatctacc aacaatgtgt gatatcagac aactactatt tgtagttgaa gttgttgata
10501 agtactttga ttgttacgat ggtggctgta ttaatgctaa ccaagtcatc gtcaacaacc
10561 tagacaaatc agctggtttt ccatttaata aatggggtaa ggctagactt tattatgatt
10621 caatgagtta tgaggatcaa gatgcacttt tcgcatatac aaaacgtaat gtcatcccta
10681 ctataactca aatgaatctt aagtatgcca ttagtgcaaa gaatagagct cgcaccgtag
10741 ctggtgtctc tatctgtagt actatgacca atagacagtt tcatcaaaaa ttattgaaat
10801 caatagccgc cactagagga gctactgtag taattggaac aagcaaattc tatggtggtt
10861 ggcacaacat gttaaaaact gtttatagtg atgtagaaaa ccctcacctt atgggttggg
10921 attatcctaa atgtgataga gccatgccta acatgcttag aattatggcc tcacttgttc
10981 ttgctcgcaa acatacaacg tgttgtagct tgtcacaccg tttctataga ttagctaatg
11041 agtgtgctca agtattgagt gaaggcatgc aagcttgata tcaagcttat cgataatcaa
11101 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
11161 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
11221 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
11281 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
11341 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
11401 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
11461 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
11521 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
11581 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
11641 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcat
//
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