lenti dCAS-VP64_Blast

价格:1200元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

买家导航

载体基本信息

质粒名称: lenti dCAS-VP64_Blast 
出品公司: 爱迪基因
目录编号: 61425
存储实验室: Feng Zhang

载体骨架基本信息
载体名称: plenti
载体抗性: 氨苄霉素
载体类型: 哺乳细胞表达
筛选标记: 稻瘟
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
插入基因名称: dCAS9(D10A, N863A)-VP64_2A_Blast
物种来源: Synthetic; S. pyogenes
基因突变: D10A and N863A in Cas9
启动子 EF1A
克隆方法: 限制性内切酶
5' 克隆位点: BsiWI(未破坏)
3' 克隆位点: EcoRI(未破坏)
5' 测序引物 GTTTGGATCTTGGTTCATTCTCAAGCCTCAG
3' 测序引物 cacatagcgtaaaaggagcaacatag

文献引用方法
材料和方法部分: lenti dCAS-VP64_Blast was a gift from Feng Zhang (Addgene plasmid # 61425)
参考文献部分: Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F. Nature. 2014 Dec 10. doi: 10.1038/nature14136. 10.1038/nature14136 PubMed 25494202

载体图谱质粒图谱和多克隆位点信息

lenti dCAS-VP64_Blast载体图谱

载体简介

载体序列

LOCUS       lenti dCAS-VP64_Blast Sequencing Result	14085 bp 	DNA	SYN
DEFINITION  lenti dCAS-VP64_Blast Sequencing Result
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..14085
                     /organism="lenti dCAS-VP64_Blast Sequencing Result"
                     /mol_type="other DNA"
     promoter        239..815
                     /label="CMV_immearly_promoter"
     misc_feature    318..605
                     /label="CAG_enhancer"
     misc_feature    772..792
                     /label="CMV_fwd_primer"
     misc_feature    835..1015
                     /label="truncHIV-1_3_LTR"
     misc_feature    835..1015
                     /label="HIV-1_5_LTR"
     misc_feature    1126..1170
                     /label="HIV-1_psi_pack"
     CDS             1558..2445
                     /label="ORF frame 1"
     misc_feature    1680..1913
                     /label="RRE"
     misc_feature    2444..2459
                     /label="cPPT"
     promoter        2694..3865
                     /label="EF1a_promoter"
     CDS             complement(2985..3605)
                     /label="ORF frame 2"
     misc_feature    3813..3833
                     /label="EF1a_fwd_primer"
     CDS             3878..8752
                     /label="ORF frame 2"
     misc_feature    8084..8104
                     /label="SV40 NLS"
     misc_feature    8090..8104
                     /label="NLS"
     gene            8354..8752
                     /label="blast"
                     /gene="blast"
     misc_feature    8779..9366
                     /label="WPRE"
     CDS             8867..9916
                     /label="ORF frame 2"
     CDS             8880..9392
                     /label="ORF frame 3"
     misc_feature    complement(9369..9385)
                     /label="pBluescriptKS_primer"
     misc_feature    9553..9568
                     /label="cPPT"
                     /label="U3PPT"
     misc_feature    9890..10070
                     /label="truncHIV-1_3_LTR"
     misc_feature    9890..10070
                     /label="HIV-1_5_LTR"
     misc_feature    complement(10096..10113)
                     /label="BGH_rev_primer"
     terminator      10099..10326
                     /label="bGH_PA_terminator"
     rep_origin      10389..10695
                     /label="f1_origin"
     misc_feature    complement(10809..10829)
                     /label="pBABE_3_primer"
     misc_feature    complement(10815..11030)
                     /label="SV40_enhancer"
     promoter        10827..11095
                     /label="SV40_promoter"
     rep_origin      10994..11071
                     /label="SV40_origin"
     misc_feature    11056..11075
                     /label="SV40pro_F_primer"
     promoter        11189..11256
                     /label="EM7_promoter"
     gene            11257..11628
                     /label="bleo"
                     /gene="bleo"
     misc_feature    11257..11631
                     /label="sh_ble"
     terminator      11764..11883
                     /label="SV40_PA_terminator"
     misc_feature    11852..11871
                     /label="EBV_rev_primer"
     promoter        complement(11927..11945)
                     /label="M13_reverse_primer"
     misc_feature    complement(11944..11966)
                     /label="M13_pUC_rev_primer"
     promoter        complement(11980..12009)
                     /label="lac_promoter"
     gene            complement(13092..13952)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(13092..13952)
                     /label="ORF frame 2"
     promoter        complement(13994..14022)
                     /label="AmpR_promoter"
ORIGIN
    1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
   61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
  121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
  181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
  241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
  301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
  361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
  421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
  481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
  541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
  601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
  661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
  721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
  781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
  841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
  901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
  961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
 2581 agatccagtt tggttaatta gctagcTGCA AAGATGGATA AAGTTTTAAA CAGAGAGGAA
 2641 TCTTTGCAGC TAATGGACCT TCTAGGTCTT GAAAGGAGTG GGAATTGGCT CCGGTGCCCG
 2701 TCAGTGGGCA GAGCGCACAT CGCCCACAGT CCCCGAGAAG TTGGGGGGAG GGGTCGGCAA
 2761 TTGAACCGGT GCCTAGAGAA GGTGGCGCGG GGTAAACTGG GAAAGTGATG TCGTGTACTG
 2821 GCTCCGCCTT TTTCCCGAGG GTGGGGGAGA ACCGTATATA AGTGCAGTAG TCGCCGTGAA
 2881 CGTTCTTTTT CGCAACGGGT TTGCCGCCAG AACACAGGTA AGTGCCGTGT GTGGTTCCCG
 2941 CGGGCCTGGC CTCTTTACGG GTTATGGCCC TTGCGTGCCT TGAATTACTT CCACCTGGCT
 3001 GCAGTACGTG ATTCTTGATC CCGAGCTTCG GGTTGGAAGT GGGTGGGAGA GTTCGAGGCC
 3061 TTGCGCTTAA GGAGCCCCTT CGCCTCGTGC TTGAGTTGAG GCCTGGCCTG GGCGCTGGGG
 3121 CCGCCGCGTG CGAATCTGGT GGCACCTTCG CGCCTGTCTC GCTGCTTTCG ATAAGTCTCT
 3181 AGCCATTTAA AATTTTTGAT GACCTGCTGC GACGCTTTTT TTCTGGCAAG ATAGTCTTGT
 3241 AAATGCGGGC CAAGATCTGC ACACTGGTAT TTCGGTTTTT GGGGCCGCGG GCGGCGACGG
 3301 GGCCCGTGCG TCCCAGCGCA CATGTTCGGC GAGGCGGGGC CTGCGAGCGC GGCCACCGAG
 3361 AATCGGACGG GGGTAGTCTC AAGCTGGCCG GCCTGCTCTG GTGCCTGGCC TCGCGCCGCC
 3421 GTGTATCGCC CCGCCCTGGG CGGCAAGGCT GGCCCGGTCG GCACCAGTTG CGTGAGCGGA
 3481 AAGATGGCCG CTTCCCGGCC CTGCTGCAGG GAGCTCAAAA TGGAGGACGC GGCGCTCGGG
 3541 AGAGCGGGCG GGTGAGTCAC CCACACAAAG GAAAAGGGCC TTTCCGTCCT CAGCCGTCGC
 3601 TTCATGTGAC TCCACGGAGT ACCGGGCGCC GTCCAGGCAC CTCGATTAGT TCTCGAGCTT
 3661 TTGGAGTACG TCGTCTTTAG GTTGGGGGGA GGGGTTTTAT GCGATGGAGT TTCCCCACAC
 3721 TGAGTGGGTG GAGACTGAAG TTAGGCCAGC TTGGCACTTG ATGTAATTCT CCTTGGAATT
 3781 TGCCCTTTTT GAGTTTGGAT CTTGGTTCAT TCTCAAGCCT CAGACAGTGG TTCAAAGTTT
 3841 TTTTCTTCCA TTTCAGGTGT CGTGAcGTAC GgccaccATG AAAAGGCCGG CGGCCACGAA
 3901 AAAGGCCGGC CAGGCAAAAA AGAAAAAGGA CAAGAAGTAC AGCATCGGCC TGGCCATCGG
 3961 CACCAACTCT GTGGGCTGGG CCGTGATCAC CGACGAGTAC AAGGTGCCCA GCAAGAAATT
 4021 CAAGGTGCTG GGCAACACCG ACCGGCACAG CATCAAGAAG AACCTGATCG GAGCCCTGCT
 4081 GTTCGACAGC GGCGAAACAG CCGAGGCCAC CCGGCTGAAG AGAACCGCCA GAAGAAGATA
 4141 CACCAGACGG AAGAACCGGA TCTGCTATCT GCAAGAGATC TTCAGCAACG AGATGGCCAA
 4201 GGTGGACGAC AGCTTCTTCC ACAGACTGGA AGAGTCCTTC CTGGTGGAAG AGGATAAGAA
 4261 GCACGAGCGG CACCCCATCT TCGGCAACAT CGTGGACGAG GTGGCCTACC ACGAGAAGTA
 4321 CCCCACCATC TACCACCTGA GAAAGAAACT GGTGGACAGC ACCGACAAGG CCGACCTGCG
 4381 GCTGATCTAT CTGGCCCTGG CCCACATGAT CAAGTTCCGG GGCCACTTCC TGATCGAGGG
 4441 CGACCTGAAC CCCGACAACA GCGACGTGGA CAAGCTGTTC ATCCAGCTGG TGCAGACCTA
 4501 CAACCAGCTG TTCGAGGAAA ACCCCATCAA CGCCAGCGGC GTGGACGCCA AGGCCATCCT
 4561 GTCTGCCAGA CTGAGCAAGA GCAGACGGCT GGAAAATCTG ATCGCCCAGC TGCCCGGCGA
 4621 GAAGAAGAAT GGCCTGTTCG GCAACCTGAT TGCCCTGAGC CTGGGCCTGA CCCCCAACTT
 4681 CAAGAGCAAC TTCGACCTGG CCGAGGATGC CAAACTGCAG CTGAGCAAGG ACACCTACGA
 4741 CGACGACCTG GACAACCTGC TGGCCCAGAT CGGCGACCAG TACGCCGACC TGTTTCTGGC
 4801 CGCCAAGAAC CTGTCCGACG CCATCCTGCT GAGCGACATC CTGAGAGTGA ACACCGAGAT
 4861 CACCAAGGCC CCCCTGAGCG CCTCTATGAT CAAGAGATAC GACGAGCACC ACCAGGACCT
 4921 GACCCTGCTG AAAGCTCTCG TGCGGCAGCA GCTGCCTGAG AAGTACAAAG AGATTTTCTT
 4981 CGACCAGAGC AAGAACGGCT ACGCCGGCTA CATTGACGGC GGAGCCAGCC AGGAAGAGTT
 5041 CTACAAGTTC ATCAAGCCCA TCCTGGAAAA GATGGACGGC ACCGAGGAAC TGCTCGTGAA
 5101 GCTGAACAGA GAGGACCTGC TGCGGAAGCA GCGGACCTTC GACAACGGCA GCATCCCCCA
 5161 CCAGATCCAC CTGGGAGAGC TGCACGCCAT TCTGCGGCGG CAGGAAGATT TTTACCCATT
 5221 CCTGAAGGAC AACCGGGAAA AGATCGAGAA GATCCTGACC TTCCGCATCC CCTACTACGT
 5281 GGGCCCTCTG GCCAGGGGAA ACAGCAGATT CGCCTGGATG ACCAGAAAGA GCGAGGAAAC
 5341 CATCACCCCC TGGAACTTCG AGGAAGTGGT GGACAAGGGC GCTTCCGCCC AGAGCTTCAT
 5401 CGAGCGGATG ACCAACTTCG ATAAGAACCT GCCCAACGAG AAGGTGCTGC CCAAGCACAG
 5461 CCTGCTGTAC GAGTACTTCA CCGTGTATAA CGAGCTGACC AAAGTGAAAT ACGTGACCGA
 5521 GGGAATGAGA AAGCCCGCCT TCCTGAGCGG CGAGCAGAAA AAGGCCATCG TGGACCTGCT
 5581 GTTCAAGACC AACCGGAAAG TGACCGTGAA GCAGCTGAAA GAGGACTACT TCAAGAAAAT
 5641 CGAGTGCTTC GACTCCGTGG AAATCTCCGG CGTGGAAGAT CGGTTCAACG CCTCCCTGGG
 5701 CACATACCAC GATCTGCTGA AAATTATCAA GGACAAGGAC TTCCTGGACA ATGAGGAAAA
 5761 CGAGGACATT CTGGAAGATA TCGTGCTGAC CCTGACACTG TTTGAGGACA GAGAGATGAT
 5821 CGAGGAACGG CTGAAAACCT ATGCCCACCT GTTCGACGAC AAAGTGATGA AGCAGCTGAA
 5881 GCGGCGGAGA TACACCGGCT GGGGCAGGCT GAGCCGGAAG CTGATCAACG GCATCCGGGA
 5941 CAAGCAGTCC GGCAAGACAA TCCTGGATTT CCTGAAGTCC GACGGCTTCG CCAACAGAAA
 6001 CTTCATGCAG CTGATCCACG ACGACAGCCT GACCTTTAAA GAGGACATCC AGAAAGCCCA
 6061 GGTGTCCGGC CAGGGCGATA GCCTGCACGA GCACATTGCC AATCTGGCCG GCAGCCCCGC
 6121 CATTAAGAAG GGCATCCTGC AGACAGTGAA GGTGGTGGAC GAGCTCGTGA AAGTGATGGG
 6181 CCGGCACAAG CCCGAGAACA TCGTGATCGA AATGGCCAGA GAGAACCAGA CCACCCAGAA
 6241 GGGACAGAAG AACAGCCGCG AGAGAATGAA GCGGATCGAA GAGGGCATCA AAGAGCTGGG
 6301 CAGCCAGATC CTGAAAGAAC ACCCCGTGGA AAACACCCAG CTGCAGAACG AGAAGCTGTA
 6361 CCTGTACTAC CTGCAGAATG GGCGGGATAT GTACGTGGAC CAGGAACTGG ACATCAACCG
 6421 GCTGTCCGAC TACGATGTGG ACCACATCGT GCCTCAGAGC TTTCTGAAGG ACGACTCCAT
 6481 CGACAACAAG GTGCTGACCA GAAGCGACAA GGCCCGGGGC AAGAGCGACA ACGTGCCCTC
 6541 CGAAGAGGTC GTGAAGAAGA TGAAGAACTA CTGGCGGCAG CTGCTGAACG CCAAGCTGAT
 6601 TACCCAGAGA AAGTTCGACA ATCTGACCAA GGCCGAGAGA GGCGGCCTGA GCGAACTGGA
 6661 TAAGGCCGGC TTCATCAAGA GACAGCTGGT GGAAACCCGG CAGATCACAA AGCACGTGGC
 6721 ACAGATCCTG GACTCCCGGA TGAACACTAA GTACGACGAG AATGACAAGC TGATCCGGGA
 6781 AGTGAAAGTG ATCACCCTGA AGTCCAAGCT GGTGTCCGAT TTCCGGAAGG ATTTCCAGTT
 6841 TTACAAAGTG CGCGAGATCA ACAACTACCA CCACGCCCAC GACGCCTACC TGAACGCCGT
 6901 CGTGGGAACC GCCCTGATCA AAAAGTACCC TAAGCTGGAA AGCGAGTTCG TGTACGGCGA
 6961 CTACAAGGTG TACGACGTGC GGAAGATGAT CGCCAAGAGC GAGCAGGAAA TCGGCAAGGC
 7021 TACCGCCAAG TACTTCTTCT ACAGCAACAT CATGAACTTT TTCAAGACCG AGATTACCCT
 7081 GGCCAACGGC GAGATCCGGA AGCGGCCTCT GATCGAGACA AACGGCGAAA CCGGGGAGAT
 7141 CGTGTGGGAT AAGGGCCGGG ATTTTGCCAC CGTGCGGAAA GTGCTGAGCA TGCCCCAAGT
 7201 GAATATCGTG AAAAAGACCG AGGTGCAGAC AGGCGGCTTC AGCAAAGAGT CTATCCTGCC
 7261 CAAGAGGAAC AGCGATAAGC TGATCGCCAG AAAGAAGGAC TGGGACCCTA AGAAGTACGG
 7321 CGGCTTCGAC AGCCCCACCG TGGCCTATTC TGTGCTGGTG GTGGCCAAAG TGGAAAAGGG
 7381 CAAGTCCAAG AAACTGAAGA GTGTGAAAGA GCTGCTGGGG ATCACCATCA TGGAAAGAAG
 7441 CAGCTTCGAG AAGAATCCCA TCGACTTTCT GGAAGCCAAG GGCTACAAAG AAGTGAAAAA
 7501 GGACCTGATC ATCAAGCTGC CTAAGTACTC CCTGTTCGAG CTGGAAAACG GCCGGAAGAG
 7561 AATGCTGGCC TCTGCCGGCG AACTGCAGAA GGGAAACGAA CTGGCCCTGC CCTCCAAATA
 7621 TGTGAACTTC CTGTACCTGG CCAGCCACTA TGAGAAGCTG AAGGGCTCCC CCGAGGATAA
 7681 TGAGCAGAAA CAGCTGTTTG TGGAACAGCA CAAGCACTAC CTGGACGAGA TCATCGAGCA
 7741 GATCAGCGAG TTCTCCAAGA GAGTGATCCT GGCCGACGCT AATCTGGACA AAGTGCTGTC
 7801 CGCCTACAAC AAGCACCGGG ATAAGCCCAT CAGAGAGCAG GCCGAGAATA TCATCCACCT
 7861 GTTTACCCTG ACCAATCTGG GAGCCCCTGC CGCCTTCAAG TACTTTGACA CCACCATCGA
 7921 CCGGAAGAGG TACACCAGCA CCAAAGAGGT GCTGGACGCC ACCCTGATCC ACCAGAGCAT
 7981 CACCGGCCTG TACGAGACAC GGATCGACCT GTCTCAGCTG GGAGGCGACa gcgctGGAGG
 8041 AGGTGGAAGC GGAGGAGGAG GAAGCGGAGG AGGAGGTAGC ggacctaaga aaaagaggaa
 8101 ggtggcggcc gctggatccG GACGGGCTGA CGCATTGGAC GATTTTGATC TGGATATGCT
 8161 GGGAAGTGAC GCCCTCGATG ATTTTGACCT TGACATGCTT GGTTCGGATG CCCTTGATGA
 8221 CTTTGACCTC GACATGCTCG GCAGTGACGC CCTTGATGAT TTCGACCTGG ACATGCTGAT
 8281 TAACtgtaca GGCAGTGGAG AGGGCAGAGG AAGTCTGCTA ACATGCGGTG ACGTCGAGGA
 8341 GAATCCTGGC CCAatggcca agcctttgtc tcaagaagaa tccaccctca ttgaaagagc
 8401 aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca gcgcagctct
 8461 ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg ggggaccttg
 8521 tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc tgacttgtat
 8581 cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt gccgacaggt
 8641 gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg gacagccgac
 8701 ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggct aagaattcga
 8761 tatcaagctt atcggtaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
 8821 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
 8881 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
 8941 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
 9001 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
 9061 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
 9121 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
 9181 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
 9241 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
 9301 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
 9361 cccgcatcga taccgtcgac ctcgagacct agaaaaacat ggagcaatca caagtagcaa
 9421 tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg
 9481 ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct
 9541 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
 9601 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta
 9661 cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc
 9721 agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc
 9781 tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag
 9841 ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg
 9901 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
 9961 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
10021 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gggcccgttt
10081 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
10141 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
10201 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
10261 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
10321 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
10381 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
10441 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
10501 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
10561 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
10621 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
10681 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
10741 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
10801 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
10861 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
10921 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
10981 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
11041 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
11101 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
11161 tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc
11221 ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt
11281 ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg
11341 ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg
11401 ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg
11461 cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac
11521 gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg
11581 cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta
11641 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
11701 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
11761 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
11821 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
11881 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
11941 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
12001 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
12061 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
12121 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
12181 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
12241 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
12301 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
12361 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
12421 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
12481 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
12541 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
12601 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
12661 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
12721 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
12781 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
12841 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
12901 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
12961 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
13021 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
13081 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
13141 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
13201 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
13261 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
13321 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
13381 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
13441 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
13501 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
13561 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
13621 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
13681 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
13741 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
13801 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
13861 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
13921 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
13981 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
14041 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgac
//

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