| 出品公司: | Bio-Rad |
|---|---|
| 载体名称: | pGLO |
| 质粒类型: | -- |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | -- |
| 启动子: | -- |
| 载体大小: | 5371 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | 调控GFP表达的细菌转化质粒。 |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pGLO 5371 bp ds-DNA circular SYN 02-MAY-2019
DEFINITION Bacterial transformation plasmid for regulated expression of GFP.
ACCESSION .
VERSION .
KEYWORDS pGLO
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5371)
AUTHORS Bio-Rad
TITLE Direct Submission
JOURNAL Exported Tuesday, February 25, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5371
/organism="synthetic DNA construct"
/lab_host="Escherichia coli"
/mol_type="other DNA"
CDS complement(96..974)
/codon_start=1
/gene="araC"
/product="L-arabinose regulatory protein"
/note="araC"
/translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
EKVNDVAVKLS"
promoter 1120..1285
/gene="araBAD"
/note="araBAD promoter"
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction"
CDS 1342..2061
/codon_start=1
/product="Aequoria victoria green fluorescent protein"
/note="GFP"
/translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITHGMDELYK"
misc_feature 2063..2119
/note="MCS"
/note="pUC18/19 multiple cloning site"
terminator 2321..2407
/gene="Escherichia coli rrnB"
/note="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
promoter 2544..2635
/gene="bla"
/note="AmpR promoter"
CDS 2636..3496
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 3538..3993
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
rep_origin 4104..4692
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac
61 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca
121 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta
181 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata
241 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag
301 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag
361 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg
421 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct
481 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc
541 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc
601 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca
661 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga
721 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa
781 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata
841 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc
901 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt
961 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat
1021 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta
1081 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt
1141 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca
1201 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta
1261 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagaaataa ttttgtttaa
1321 ctttaagaag gagatataca tatggctagc aaaggagaag aacttttcac tggagttgtc
1381 ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag
1441 ggtgaaggtg atgctacata cggaaagctt acccttaaat ttatttgcac tactggaaaa
1501 ctacctgttc catggccaac acttgtcact actttctctt atggtgttca atgcttttcc
1561 cgttatccgg atcatatgaa acggcatgac tttttcaaga gtgccatgcc cgaaggttat
1621 gtacaggaac gcactatatc tttcaaagat gacgggaact acaagacgcg tgctgaagtc
1681 aagtttgaag gtgataccct tgttaatcgt atcgagttaa aaggtattga ttttaaagaa
1741 gatggaaaca ttctcggaca caaactcgag tacaactata actcacacaa tgtatacatc
1801 acggcagaca aacaaaagaa tggaatcaaa gctaacttca aaattcgcca caacattgaa
1861 gatggatccg ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct
1921 gtccttttac cagacaacca ttacctgtcg acacaatctg ccctttcgaa agatcccaac
1981 gaaaagcgtg accacatggt ccttcttgag tttgtaactg ctgctgggat tacacatggc
2041 atggatgagc tctacaaata atgaattcga gctcggtacc cggggatcct ctagagtcga
2101 cctgcaggca tgcaagcttg gctgttttgg cggatgagag aagattttca gcctgataca
2161 gattaaatca gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc
2221 ggtggtccca cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag
2281 tgtggggtcc cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca
2341 gtgcaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag
2401 gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc
2461 aggacgcccg ccataaactg ccaggcatca aattaagcag aaggccatcc tgacggatgg
2521 cctttttgcg tttctacaaa ctctttgttt atttttctaa atacattcaa atatgtatcc
2581 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
2641 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
2701 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
2761 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
2821 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt
2881 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
2941 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
3001 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
3061 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
3121 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc
3181 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
3241 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
3301 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
3361 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
3421 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
3481 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttacg
3541 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
3601 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
3661 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
3721 ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat
3781 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
3841 tcttgttcca aactggaaca acactcaacc ctatctcggg ctattctttt gatttataag
3901 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
3961 cgaattttaa caaaatatta acgtttacaa tttaaaagga tctaggtgaa gatccttttt
4021 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
4081 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
4141 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
4201 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
4261 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
4321 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
4381 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
4441 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga
4501 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
4561 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
4621 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
4681 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
4741 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
4801 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
4861 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
4921 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
4981 acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg
5041 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
5101 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc
5161 aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa
5221 caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata
5281 taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag
5341 aggatctaat tctcatgttt gacagcttat c
//
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