pCMV_UdgX-APOBEC1-UdgX-HF-NG-nCas9

LOCUS       pCMV_UdgX-APOBEC1-UdgX-HF-NG-nCas9               9849 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Mammalian CG-to-GC base editing.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV_UdgX-APOBEC1-UdgX-HF-NG-nCas9
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9849)
  TITLE     Liu Lab CGBE base editors
REFERENCE   2  (bases 1 to 9849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9849
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             694..714
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1438..2121
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="SSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGG
                     RHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFLS
                     RYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSNE
                     AHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHIL
                     WATGLK"
     CDS             2941..4656
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(N)
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTAFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
     CDS             7084..7104
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7113..7130
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7153..7170)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7159..7383
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7454..7470)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7454..7470)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7467..7489)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7478..7494
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7502..7532)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7547..7568
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7685..7702)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7856..8444)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7936..7955)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8615..9475)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9238..9257
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9476..9580)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9659..9678)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      601 agatccgcta gagatccgcg gccgctaata cgactcacta tagggagagc cgccaccatg
      661 aaacggacag ccgacggaag cgagttcgag tcaccaaaga agaagcggaa agtcatggcc
      721 ggagcacagg attttgtccc acatactgcc gatctggctg agctggctgc cgccgctgga
      781 gagtgtagag gatgcgggct gtaccgggat gccacacagg ccgtgttcgg agcaggcggc
      841 aggagcgccc gcatcatgat gatcggcgag cagccaggcg ataaggagga cctggccggc
      901 ctgccatttg tgggaccagc aggccggctg ctggacagag ccctggaggc cgccgacatc
      961 gatagggacg ccctgtacgt gaccaacgcc gtgaagcact tcaagtttac acgcgccgca
     1021 ggaggcaaga ggagaatcca caagaccccc tctcggacag aggtggtggc ctgcagacct
     1081 tggctgatcg ccgagatgac cagcgtggag ccagatgtgg tggtgctgct gggagcaaca
     1141 gcagcaaagg ccctgctggg caatgacttc agggtgaccc agcacagggg agaggtgctg
     1201 cacgtggacg atgtgccagg cgatcctgcc ctggtggcaa ccgtgcaccc tagctccctg
     1261 ctgagaggcc caaaggagga gcgggaatcc gccttcgctg ggctggtgga cgacctgaga
     1321 gtggctgccg atgtgagacc ttctggagga tctagcggag gctcctctgg cagcgagaca
     1381 ccaggaacaa gcgagtcagc aacaccagag agcagtggcg gcagcagcgg ggggtcatcc
     1441 tcagagactg ggcctgtcgc cgtcgatcca accctgcgcc gccggattga acctcacgag
     1501 tttgaagtgt tctttgaccc ccgggagctg agaaaggaga catgcctgct gtacgagatc
     1561 aactggggag gcaggcactc catctggagg cacacctctc agaacacaaa taagcacgtg
     1621 gaggtgaact tcatcgagaa gtttaccaca gagcggtact tctgccccaa taccagatgt
     1681 agcatcacat ggtttctgag ctggtcccct tgcggagagt gtagcagggc catcaccgag
     1741 ttcctgtcca gatatccaca cgtgacactg tttatctaca tcgccaggct gtatcaccac
     1801 gcagacccaa ggaataggca gggcctgcgc gatctgatca gctccggcgt gaccatccag
     1861 atcatgacag agcaggagtc cggctactgc tggcggaact tcgtgaatta ttctcctagc
     1921 aacgaggccc actggcctag gtacccacac ctgtgggtgc gcctgtacgt gctggagctg
     1981 tattgcatca tcctgggcct gcccccttgt ctgaatatcc tgcggagaaa gcagccccag
     2041 ctgaccttct ttacaatcgc cctgcagtct tgtcactatc agaggctgcc accccacatc
     2101 ctgtgggcca caggcctgaa gtctggagga tctagcggag gatcctctgg cagcgagaca
     2161 ccaggaacaa gcgagtcagc aacaccagag agcagtggcg gcagcagcgg cggcagcatg
     2221 gccggagcac aggattttgt cccacatact gccgatctgg ctgagctggc tgccgccgct
     2281 ggagagtgta gaggatgcgg gctgtaccgg gatgcaacac aggccgtgtt cggagcaggc
     2341 ggcaggagcg cccgcatcat gatgatcggc gagcagccag gcgataagga ggacctggcc
     2401 ggcctgccat ttgtgggacc agcaggccgg ctgctggaca gagccctgga ggccgccgac
     2461 atcgataggg acgccctgta cgtgaccaac gccgtgaagc acttcaagtt tacacgcgcc
     2521 gcaggaggca agaggagaat ccacaagacc ccctctcgga cagaggtggt ggcctgcaga
     2581 ccttggctga tcgccgagat gaccagcgtg gagccagatg tggtggtgct gctgggagca
     2641 acagcagcaa aggccctgct gggcaatgac ttcagggtga cccagcacag gggagaggtg
     2701 ctgcacgtgg acgatgtgcc aggcgatcct gccctggtgg caaccgtgca ccctagctcc
     2761 ctgctgagag gcccaaagga ggagcgggaa tccgccttcg ctgggctggt ggacgacctg
     2821 agagtggctg ccgatgtgag acctagcggt ggtagttctg gcggtagctc aggatctgaa
     2881 actcccggca catctgaaag cgcgactccc gaatcatctg gtggatcttc aggtggaagt
     2941 gacaagaagt acagcatcgg cctggccatc ggcaccaact ctgtgggctg ggccgtgatc
     3001 accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac
     3061 agcatcaaga agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgaggcc
     3121 acccggctga agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat
     3181 ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg
     3241 gaagagtcct tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac
     3301 atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa
     3361 ctggtggaca gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg
     3421 atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg
     3481 gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc
     3541 aacgccagcg gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg
     3601 ctggaaaatc tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggaaacctg
     3661 attgccctga gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat
     3721 gccaaactgc agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag
     3781 atcggcgacc agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg
     3841 ctgagcgaca tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg
     3901 atcaagagat acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag
     3961 cagctgcctg agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc
     4021 tacattgacg gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa
     4081 aagatggacg gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag
     4141 cagcggacct tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc
     4201 attctgcggc ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag
     4261 aagatcctga ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga
     4321 ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg
     4381 gtggacaagg gcgcttccgc ccagagcttc atcgagcgga tgaccgcctt cgataagaac
     4441 ctgcccaacg agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtat
     4501 aacgagctga ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc
     4561 ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg
     4621 aagcagctga aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc
     4681 ggcgtggaag atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc
     4741 aaggacaagg acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg
     4801 accctgacac tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac
     4861 ctgttcgacg acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg
     4921 ctgagcgcga agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat
     4981 ttcctgaagt ccgacggctt cgccaacaga aacttcatgg cgctgatcca cgacgacagc
     5041 ctgaccttta aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac
     5101 gagcacattg ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg
     5161 aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc
     5221 gaaatggcca gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg
     5281 aagcggatcg aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg
     5341 gaaaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat
     5401 atgtacgtgg accaggaact ggacatcaac cggctgtccg actacgatgt ggaccatatc
     5461 gtgcctcaga gctttctgaa ggacgactcc atcgacaaca aggtgctgac cagaagcgac
     5521 aagaaccggg gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac
     5581 tactggcggc agctgctgaa cgccaagctg attacccaga gaaagttcga caatctgacc
     5641 aaggccgaga gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg
     5701 gtggaaaccc gggcgatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact
     5761 aagtacgacg agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag
     5821 ctggtgtccg atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac
     5881 caccacgccc acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac
     5941 cctaagctgg aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg
     6001 atcgccaaga gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac
     6061 atcatgaact ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct
     6121 ctgatcgaga caaacggcga aaccggggag atcgtgtggg ataagggccg ggattttgcc
     6181 accgtgcgga aagtgctgag catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag
     6241 acaggcggct tcagcaaaga gtctatcagg cccaagagga acagcgataa gctgatcgcc
     6301 agaaagaagg actgggaccc taagaagtac ggcggcttcg tcagccccac cgtggcctat
     6361 tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa
     6421 gagctgctgg ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt
     6481 ctggaagcca agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac
     6541 tccctgttcg agctggaaaa cggccggaag agaatgctgg cctctgccag attcctgcag
     6601 aagggaaacg aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac
     6661 tatgagaagc tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag
     6721 cacaagcact acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc
     6781 ctggccgacg ctaatctgga caaagtgctg tccgcctaca acaagcaccg ggataagccc
     6841 atcagagagc aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct
     6901 agggccttca agtactttga caccaccatc gaccggaagg tgtacaggag caccaaagag
     6961 gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac
     7021 ctgtctcagc tgggaggtga ctctggcggc tcaaaaagaa ccgccgacgg cagcgaattc
     7081 gagcccaaga agaagaggaa agtctaaccg gtcatcatca ccatcaccat tgagtttaaa
     7141 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     7201 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     7261 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     7321 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     7381 tggcttctga ggcggaaaga accagctggg gctcgatacc gtcgacctct agctagagct
     7441 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     7501 acaacatacg agccggaagc ataaagtgta aagcctaggg tgcctaatga gtgagctaac
     7561 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     7621 tgcattaatg aatcggccaa cgcgcgggaa gaggcggttt gcgtattggg cgctcttccg
     7681 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     7741 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     7801 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     7861 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     7921 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     7981 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     8041 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     8101 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     8161 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     8221 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     8281 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     8341 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     8401 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     8461 tttctacggg gtctgacact cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     8521 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     8581 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     8641 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     8701 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     8761 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     8821 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     8881 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     8941 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     9001 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     9061 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     9121 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     9181 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     9241 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     9301 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     9361 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     9421 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     9481 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     9541 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     9601 cacctgacgt cgacggatcg ggagatcgat ctcccgatcc cctagggtcg actctcagta
     9661 caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg
     9721 tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt
     9781 gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat
     9841 atacgcgtt
//