| 质粒名称: | pCMV_UdgX-APOBEC1-UdgX-HF-NG-nCas9 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 163561 |
| 存储实验室: | David Liu |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达,CRISPR |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | UdgX-APOBEC1-UdgX-HF-NG-nCas9 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | nCas9 (D10A)N497A, R661A, Q695A, Q926A//L111R, D1135V, G1218R, E1219F, A1322R, R1335V, T1337R |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gibson克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pCMV_UdgX-APOBEC1-UdgX-HF-NG-nCas9 was a gift from David Liu (Addgene plasmid # 163561 ; http://n2t.net/addgene:163561 ; RRID:Addgene_163561) |
| 参考文献部分: | Efficient C*G-to-G*C base editors developed using CRISPRi screens, target-library analysis, and machine learning. Koblan LW, Arbab M, Shen MW, Hussmann JA, Anzalone AV, Doman JL, Newby GA, Yang D, Mok B, Replogle JM, Xu A, Sisley TA, Weissman JS, Adamson B, Liu DR. Nat Biotechnol. 2021 Jun 28. pii: 10.1038/s41587-021-00938-z. doi: 10.1038/s41587-021-00938-z. 10.1038/s41587-021-00938-z PubMed 34183861 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCMV_UdgX-APOBEC1-UdgX-HF-NG-nCas9 9849 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Mammalian CG-to-GC base editing.
ACCESSION .
VERSION .
KEYWORDS pCMV_UdgX-APOBEC1-UdgX-HF-NG-nCas9
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9849)
TITLE Liu Lab CGBE base editors
REFERENCE 2 (bases 1 to 9849)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9849
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 1..380
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 381..584
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 535..555
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 626..645
/label=T7
/note="T7 promoter, forward primer"
promoter 626..644
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 694..714
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 1438..2121
/codon_start=1
/product="cytidine deaminase (C to U editing enzyme) from
rat"
/label=APOBEC-1
/note="can use ssDNA as a substrate (Komor et al., 2016)"
/translation="SSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGG
RHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFLS
RYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSNE
AHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHIL
WATGLK"
CDS 2941..4656
/codon_start=1
/product="N-terminal portion of Streptococcus pyogenes Cas9
(Zetsche et al., 2015)"
/label=Cas9(N)
/translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTAFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
CDS 7084..7104
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 7113..7130
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(7153..7170)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 7159..7383
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(7454..7470)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7454..7470)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7467..7489)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7478..7494
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7502..7532)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7547..7568
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7685..7702)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7856..8444)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7936..7955)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8615..9475)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9238..9257
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9476..9580)
/gene="bla"
/label=AmpR promoter
primer_bind complement(9659..9678)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
601 agatccgcta gagatccgcg gccgctaata cgactcacta tagggagagc cgccaccatg
661 aaacggacag ccgacggaag cgagttcgag tcaccaaaga agaagcggaa agtcatggcc
721 ggagcacagg attttgtccc acatactgcc gatctggctg agctggctgc cgccgctgga
781 gagtgtagag gatgcgggct gtaccgggat gccacacagg ccgtgttcgg agcaggcggc
841 aggagcgccc gcatcatgat gatcggcgag cagccaggcg ataaggagga cctggccggc
901 ctgccatttg tgggaccagc aggccggctg ctggacagag ccctggaggc cgccgacatc
961 gatagggacg ccctgtacgt gaccaacgcc gtgaagcact tcaagtttac acgcgccgca
1021 ggaggcaaga ggagaatcca caagaccccc tctcggacag aggtggtggc ctgcagacct
1081 tggctgatcg ccgagatgac cagcgtggag ccagatgtgg tggtgctgct gggagcaaca
1141 gcagcaaagg ccctgctggg caatgacttc agggtgaccc agcacagggg agaggtgctg
1201 cacgtggacg atgtgccagg cgatcctgcc ctggtggcaa ccgtgcaccc tagctccctg
1261 ctgagaggcc caaaggagga gcgggaatcc gccttcgctg ggctggtgga cgacctgaga
1321 gtggctgccg atgtgagacc ttctggagga tctagcggag gctcctctgg cagcgagaca
1381 ccaggaacaa gcgagtcagc aacaccagag agcagtggcg gcagcagcgg ggggtcatcc
1441 tcagagactg ggcctgtcgc cgtcgatcca accctgcgcc gccggattga acctcacgag
1501 tttgaagtgt tctttgaccc ccgggagctg agaaaggaga catgcctgct gtacgagatc
1561 aactggggag gcaggcactc catctggagg cacacctctc agaacacaaa taagcacgtg
1621 gaggtgaact tcatcgagaa gtttaccaca gagcggtact tctgccccaa taccagatgt
1681 agcatcacat ggtttctgag ctggtcccct tgcggagagt gtagcagggc catcaccgag
1741 ttcctgtcca gatatccaca cgtgacactg tttatctaca tcgccaggct gtatcaccac
1801 gcagacccaa ggaataggca gggcctgcgc gatctgatca gctccggcgt gaccatccag
1861 atcatgacag agcaggagtc cggctactgc tggcggaact tcgtgaatta ttctcctagc
1921 aacgaggccc actggcctag gtacccacac ctgtgggtgc gcctgtacgt gctggagctg
1981 tattgcatca tcctgggcct gcccccttgt ctgaatatcc tgcggagaaa gcagccccag
2041 ctgaccttct ttacaatcgc cctgcagtct tgtcactatc agaggctgcc accccacatc
2101 ctgtgggcca caggcctgaa gtctggagga tctagcggag gatcctctgg cagcgagaca
2161 ccaggaacaa gcgagtcagc aacaccagag agcagtggcg gcagcagcgg cggcagcatg
2221 gccggagcac aggattttgt cccacatact gccgatctgg ctgagctggc tgccgccgct
2281 ggagagtgta gaggatgcgg gctgtaccgg gatgcaacac aggccgtgtt cggagcaggc
2341 ggcaggagcg cccgcatcat gatgatcggc gagcagccag gcgataagga ggacctggcc
2401 ggcctgccat ttgtgggacc agcaggccgg ctgctggaca gagccctgga ggccgccgac
2461 atcgataggg acgccctgta cgtgaccaac gccgtgaagc acttcaagtt tacacgcgcc
2521 gcaggaggca agaggagaat ccacaagacc ccctctcgga cagaggtggt ggcctgcaga
2581 ccttggctga tcgccgagat gaccagcgtg gagccagatg tggtggtgct gctgggagca
2641 acagcagcaa aggccctgct gggcaatgac ttcagggtga cccagcacag gggagaggtg
2701 ctgcacgtgg acgatgtgcc aggcgatcct gccctggtgg caaccgtgca ccctagctcc
2761 ctgctgagag gcccaaagga ggagcgggaa tccgccttcg ctgggctggt ggacgacctg
2821 agagtggctg ccgatgtgag acctagcggt ggtagttctg gcggtagctc aggatctgaa
2881 actcccggca catctgaaag cgcgactccc gaatcatctg gtggatcttc aggtggaagt
2941 gacaagaagt acagcatcgg cctggccatc ggcaccaact ctgtgggctg ggccgtgatc
3001 accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac
3061 agcatcaaga agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgaggcc
3121 acccggctga agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat
3181 ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg
3241 gaagagtcct tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac
3301 atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa
3361 ctggtggaca gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg
3421 atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg
3481 gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc
3541 aacgccagcg gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg
3601 ctggaaaatc tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggaaacctg
3661 attgccctga gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat
3721 gccaaactgc agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag
3781 atcggcgacc agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg
3841 ctgagcgaca tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg
3901 atcaagagat acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag
3961 cagctgcctg agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc
4021 tacattgacg gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa
4081 aagatggacg gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag
4141 cagcggacct tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc
4201 attctgcggc ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag
4261 aagatcctga ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga
4321 ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg
4381 gtggacaagg gcgcttccgc ccagagcttc atcgagcgga tgaccgcctt cgataagaac
4441 ctgcccaacg agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtat
4501 aacgagctga ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc
4561 ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg
4621 aagcagctga aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc
4681 ggcgtggaag atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc
4741 aaggacaagg acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg
4801 accctgacac tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac
4861 ctgttcgacg acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg
4921 ctgagcgcga agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat
4981 ttcctgaagt ccgacggctt cgccaacaga aacttcatgg cgctgatcca cgacgacagc
5041 ctgaccttta aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac
5101 gagcacattg ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg
5161 aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc
5221 gaaatggcca gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg
5281 aagcggatcg aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg
5341 gaaaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat
5401 atgtacgtgg accaggaact ggacatcaac cggctgtccg actacgatgt ggaccatatc
5461 gtgcctcaga gctttctgaa ggacgactcc atcgacaaca aggtgctgac cagaagcgac
5521 aagaaccggg gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac
5581 tactggcggc agctgctgaa cgccaagctg attacccaga gaaagttcga caatctgacc
5641 aaggccgaga gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg
5701 gtggaaaccc gggcgatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact
5761 aagtacgacg agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag
5821 ctggtgtccg atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac
5881 caccacgccc acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac
5941 cctaagctgg aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg
6001 atcgccaaga gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac
6061 atcatgaact ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct
6121 ctgatcgaga caaacggcga aaccggggag atcgtgtggg ataagggccg ggattttgcc
6181 accgtgcgga aagtgctgag catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag
6241 acaggcggct tcagcaaaga gtctatcagg cccaagagga acagcgataa gctgatcgcc
6301 agaaagaagg actgggaccc taagaagtac ggcggcttcg tcagccccac cgtggcctat
6361 tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa
6421 gagctgctgg ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt
6481 ctggaagcca agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac
6541 tccctgttcg agctggaaaa cggccggaag agaatgctgg cctctgccag attcctgcag
6601 aagggaaacg aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac
6661 tatgagaagc tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag
6721 cacaagcact acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc
6781 ctggccgacg ctaatctgga caaagtgctg tccgcctaca acaagcaccg ggataagccc
6841 atcagagagc aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct
6901 agggccttca agtactttga caccaccatc gaccggaagg tgtacaggag caccaaagag
6961 gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac
7021 ctgtctcagc tgggaggtga ctctggcggc tcaaaaagaa ccgccgacgg cagcgaattc
7081 gagcccaaga agaagaggaa agtctaaccg gtcatcatca ccatcaccat tgagtttaaa
7141 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
7201 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
7261 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
7321 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
7381 tggcttctga ggcggaaaga accagctggg gctcgatacc gtcgacctct agctagagct
7441 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
7501 acaacatacg agccggaagc ataaagtgta aagcctaggg tgcctaatga gtgagctaac
7561 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
7621 tgcattaatg aatcggccaa cgcgcgggaa gaggcggttt gcgtattggg cgctcttccg
7681 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
7741 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
7801 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
7861 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
7921 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
7981 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
8041 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
8101 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
8161 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
8221 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
8281 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
8341 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
8401 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
8461 tttctacggg gtctgacact cagtggaacg aaaactcacg ttaagggatt ttggtcatga
8521 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
8581 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
8641 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
8701 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
8761 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
8821 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
8881 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
8941 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
9001 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
9061 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
9121 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
9181 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
9241 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
9301 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
9361 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
9421 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
9481 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
9541 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
9601 cacctgacgt cgacggatcg ggagatcgat ctcccgatcc cctagggtcg actctcagta
9661 caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg
9721 tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt
9781 gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat
9841 atacgcgtt
//
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