pEarleyGate101

价格:2000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: CAMBIA
载体名称: pEarleyGate101; pEarleyGate 101
质粒类型: Gateway载体;植物载体;农杆菌双元表达载体
高拷贝/低拷贝: 高拷贝
克隆方法: Gateway
启动子: 35S
载体大小: 12454 bp
5' 测序引物及序列: 35S promoter:CTATCCTTCGCAAGACCCTTC
3' 测序引物及序列: --
载体标签: 黄色荧光蛋白EYFP、HA (C ter)
载体抗性: 卡那霉素、氯霉素(仅空载体)
筛选标记: 草丁膦(磷丝菌素)phosphinothricin、
克隆菌株: DB3.1菌株
宿主细胞(系): 植物细胞、农杆菌
备注: --
产品目录号: --
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pEarleyGate101载体图谱



载体简介

载体序列

LOCUS       pEarleyGate 101        12454 bp ds-DNA     circular SYN 26-JUL-2016
DEFINITION  Gateway(R)-compatible plant transformation vector with YFP and HA 
            C-terminal tags.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12454)
  AUTHORS   Earley KW, Haag JR, Pontes O, Opper K, Juehne T, Song K, Pikaard CS.
  TITLE     Gateway-compatible vectors for plant functional genomics and 
            proteomics.
  JOURNAL   Plant J. 2006;45:616-29.
  PUBMED    16441352
REFERENCE   2  (bases 1 to 12454)
  AUTHORS   Pikaard Lab
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, July 26, 2016 from SnapGene Viewer 3.1.4 to Vector
            NTI(R) format
            http://www.snapgene.com
COMMENT     The sequence was corrected by inserting a G at position 8562.
COMMENT     VNTDATE|659923200|
COMMENT     VNTDBDATE|776390400|
COMMENT     VNTNAME|pEarleyGate 101|
COMMENT     VNTAUTHORNAME|Pikaard Lab|
FEATURES             Location/Qualifiers
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      111..363
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(373..924)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
     promoter        complement(930..1310)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     promoter        2306..2651
                     /label=35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    2663..2782
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2812..2842
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2896..3555
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
     CDS             3897..4202
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
     protein_bind    complement(4243..4367)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             4375..5088
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
     CDS             5167..5193
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
     terminator      5225..5932
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     misc_feature    6176..6200
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7500..8129
                     /codon_start=1
                     /product="stability protein from Pseudomonas plasmid pVS1"
                     /label=pVS1 StaA
     CDS             8558..9631
                     /codon_start=1
                     /product="replication protein from Pseudomonas plasmid 
                     pVS1"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9697..9891
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    10235..10375
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(10561..11149)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11236..12030)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tccaatccca caaaaatctg agcttaacag cacagttgct cctctcagag cagaatcggg
     1381 tattcaacac cctcatatca actactacgt tgtgtataac ggtccacatg ccggtatata
     1441 cgatgactgg ggttgtacaa aggcggcaac aaacggcgtt cccggagttg cacacaagaa
     1501 atttgccact attacagagg caagagcagc agctgacgcg tacacaacaa gtcagcaaac
     1561 agacaggttg aacttcatcc ccaaaggaga agctcaactc aagcccaaga gctttgctaa
     1621 ggccctaaca agcccaccaa agcaaaaagc ccactggctc acgctaggaa ccaaaaggcc
     1681 cagcagtgat ccagccccaa aagagatctc ctttgccccg gagattacaa tggacgattt
     1741 cctctatctt tacgatctag gaaggaagtt cgaaggtgaa ggtgacgaca ctatgttcac
     1801 cactgataat gagaaggtta gcctcttcaa tttcagaaag aatgctgacc cacagatggt
     1861 tagagaggcc tacgcagcag gtctcatcaa gacgatctac ccgagtaaca atctccagga
     1921 gatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga ctaattgcat
     1981 caagaacaca gagaaagaca tatttctcaa gatcagaagt actattccag tatggacgat
     2041 tcaaggcttg cttcataaac caaggcaagt aatagagatt ggagtctcta aaaaggtagt
     2101 tcctactgaa tctaaggcca tgcatggagt ctaagattca aatcgaggat ctaacagaac
     2161 tcgccgtgaa gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga
     2221 aaatcttcgt caacatggtg gagcacgaca ctctggtcta ctccaaaaat gtcaaagata
     2281 cagtctcaga agaccaaagg gctattgaga cttttcaaca aaggataatt tcgggaaacc
     2341 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag
     2401 gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa gatctctctg
     2461 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
     2521 ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac gtaagggatg
     2581 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
     2641 tggagaggac acgctcgaga tcacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
     2701 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
     2761 taaaacacaa catatccagt catattggcg gccgcattag gcaccccagg ctttacactt
     2821 tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga
     2881 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
     2941 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
     3001 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
     3061 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg
     3121 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
     3181 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
     3241 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
     3301 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt
     3361 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa
     3421 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt
     3481 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
     3541 cagggcgggg cgtaaacgcg tggatccggc ttactaaaag ccagataaca gtatgcgtat
     3601 ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca
     3661 aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt
     3721 tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga
     3781 agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt
     3841 cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct ggtgaaatgc
     3901 agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga
     3961 gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc
     4021 tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc
     4081 gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg
     4141 atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat
     4201 aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt
     4261 tgtgttttac agcattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata
     4321 tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggtgcc tagggtgagc
     4381 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     4441 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     4501 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     4561 accttcggct acggcctgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac
     4621 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     4681 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     4741 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     4801 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     4861 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     4921 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     4981 taccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     5041 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagtc cggactcaga
     5101 tctcgagctc aagcttcgaa ttctgcagtc gacggtaccg cgggcccggg atccaccgga
     5161 tctagatacc catacgatgt tccagattac gcttaattaa gacccgggac tagtccctag
     5221 agtcctgctt taatgagata tgcgagacgc ctatgatcgc atgatatttg ctttcaattc
     5281 tgttgtgcac gttgtaaaaa acctgagcat gtgtagctca gatccttacc gccggtttcg
     5341 gttcattcta atgaatatat cacccgttac tatcgtattt ttatgaataa tattctccgt
     5401 tcaatttact gattgtaccc tactacttat atgtacaata ttaaaatgaa aacaatatat
     5461 tgtgctgaat aggtttatag cgacatctat gatagagcgc cacaataaca aacaattgcg
     5521 ttttattatt acaaatccaa ttttaaaaaa agcggcagaa ccggtcaaac ctaaaagact
     5581 gattacataa atcttattca aatttcaaaa gtgccccagg ggctagtatc tacgacacac
     5641 cgagcggcga actaataacg ctcactgaag ggaactccgg ttccccgccg gcgcgcatgg
     5701 gtgagattcc ttgaagttga gtattggccg tccgctctac cgaaagttac gggcaccatt
     5761 caacccggtc cagcacggcg gccgggtaac cgacttgctg ccccgagaat tatgcagcat
     5821 ttttttggtg tatgtgggcc ccaaatgaag tgcaggtcaa accttgacag tgacgacaaa
     5881 tcgttgggcg ggtccagggc gaattttgcg acaacatgtc gaggctcagc aggacctgca
     5941 ggcatgcaag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     6001 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     6061 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgctagagca
     6121 gcttgagctt ggatcagatt gtcgtttccc gccttcagtt taaactatca gtgtttgaca
     6181 ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataac ggatatttaa
     6241 aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc
     6301 ccctcgggat caaagtactt tgatccaacc cctccgctgc tatagtgcag tcggcttctg
     6361 acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca
     6421 ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc gcataaagta
     6481 gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg ccgctggcct
     6541 gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac gggccgaact
     6601 gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca ggcgcgaccg
     6661 cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga cagtgaccag
     6721 gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc gcatccagga
     6781 ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca cgccggccgg
     6841 ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc taatcatcga
     6901 ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc
     6961 taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac
     7021 cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc gcgcacttga
     7081 gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc
     7141 attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg aacaagcatg
     7201 aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat cgaggcggag
     7261 atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat
     7321 gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag cttggccgct
     7381 gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt
     7441 catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa ggggaacgca
     7501 tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc atcgcaaccc
     7561 atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat tccgatcccc
     7621 agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc gttgtcggca
     7681 tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga
     7741 tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg
     7801 tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac ctggtggagc
     7861 tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc
     7921 gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc gggtacgagc
     7981 tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact gccgccgccg
     8041 gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg
     8101 ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag
     8161 cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg caacgttggc
     8221 cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg cggaggatca
     8281 caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc tgctatctga
     8341 atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga tgaattttag
     8401 cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc
     8461 ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgtc tgccggccct
     8521 gcaatggcac tggaaccccc aagcccgagg aatcggcgtg agcggtcgca aaccatccgg
     8581 cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggccgcgca
     8641 ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc
     8701 cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc cgtcgattag
     8761 gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg
     8821 cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg
     8881 acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg
     8941 gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct
     9001 aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg
     9061 tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga
     9121 cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa
     9181 gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta
     9241 caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagctgattg
     9301 gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta
     9361 ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg
     9421 caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga
     9481 gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta
     9541 cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct
     9601 gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc
     9661 cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt acattgggaa
     9721 cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc cgtacattgg
     9781 gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt tttccgccta
     9841 aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg
     9901 ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg
     9961 ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg
    10021 gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc
    10081 acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
    10141 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
    10201 agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg
    10261 atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
    10321 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc
    10381 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
    10441 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
    10501 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
    10561 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
    10621 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
    10681 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
    10741 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
    10801 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    10861 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    10921 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    10981 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
    11041 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    11101 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    11161 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    11221 catgcattct aggtactaaa acaattcatc cagtaaaata taatatttta ttttctccca
    11281 atcaggcttg atccccagta agtcaaaaaa tagctcgaca tactgttctt ccccgatatc
    11341 ctccctgatc gaccggacgc agaaggcaat gtcataccac ttgtccgccc tgccgcttct
    11401 cccaagatca ataaagccac ttactttgcc atctttcaca aagatgttgc tgtctcccag
    11461 gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa aatcatacag
    11521 ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca catcggccag
    11581 atcgttattc agtaagtaat ccaattcggc taagcggctg tctaagctat tcgtataggg
    11641 acaatccgat atgtcgatgg agtgaaagag cctgatgcac tccgcataca gctcgataat
    11701 cttttcaggg ctttgttcat cttcatactc ttccgagcaa aggacgccat cggcctcact
    11761 catgagcaga ttgctccagc catcatgccg ttcaaagtgc aggacctttg gaacaggcag
    11821 ctttccttcc agccatagca tcatgtcctt ttcccgttcc acatcatagg tggtcccttt
    11881 ataccggctg tccgtcattt ttaaatatag gttttcattt tctcccacca gcttatatac
    11941 cttagcagga gacattcctt ccgtatcttt tacgcagcgg tatttttcga tcagtttttt
    12001 caattccggt gatattctca ttttagccat ttattatttc cttcctcttt tctacagtat
    12061 ttaaagatac cccaagaagc taattataac aagacgaact ccaattcact gttccttgca
    12121 ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat
    12181 aacatagtat cgacggagcc gattttgaaa ccgcggtgat cacaggcagc aacgctctgt
    12241 catcgttaca atcaacatgc taccctccgc gagatcatcc gtgtttcaaa cccggcagct
    12301 tagttgccgt tcttccgaat agcatcggta acatgagcaa agtctgccgc cttacaacgg
    12361 ctctcccgct gacgccgtcc cggactgatg ggctgcctgt atcgagtggt gattttgtgc
    12421 cgagctgccg gtcggggagc tgttggctgg ctgg
//

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