| 质粒名称: | PX458-mcherry |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 161974 |
| 存储实验室: | Joanna Wysocka |
|
载体骨架基本信息 |
|
| 载体名称: | PX458 |
| 载体出品公司: | Clontech |
| 空载体大小: | 4218bp |
| 完整质粒大小: | 9280bp |
| 载体类型: | 哺乳细胞表达, CRISPR |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Cas9-t2a-mcherry |
| 物种来源: | Streptococcus pyogene |
| 插入大小: | 5052bp |
| 启动子: | Cbh |
| 标签/融合蛋白: | 人细胞色素c氧化酶亚基VIII（N端） |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | AgeI (not destroyed) |
| 3’克隆位点: | EcoRI (not destroyed) |
| 5’测序引物 | AAGGGATGGTTGGTTGGTGG |
| 3’测序引物 |
GGCAAACAACAGATGGCTGG
(Common Sequencing Primers)
|
|
文献引用方法 |
|
| 材料和方法部分: | PX458-mcherry was a gift from Joanna Wysocka (Addgene plasmid # 161974 ; http://n2t.net/addgene:161974 ; RRID:Addgene_161974) |
| 参考文献部分: | Opposing Effects of Cohesin and Transcription on CTCF Organization Revealed by Super-resolution Imaging. Gu B, Comerci CJ, McCarthy DG, Saurabh S, Moerner WE, Wysocka J. Mol Cell. 2020 Nov 19;80(4):699-711.e7. doi: 10.1016/j.molcel.2020.10.001. Epub 2020 Oct 21. 10.1016/j.molcel.2020.10.001 PubMed 33091336 |
买家导航
PX458-mcherry载体质粒基本信息
PX458-mcherry质粒图谱载体图谱和PX458-mcherry载体序列质粒序列多克隆位点信息
PX458-mcherry质粒载体简介
PX458-mcherry质粒序列载体序列
LOCUS PX458-mcherry 9279 bp DNA circular SYN 25-AUG-2024
DEFINITION Expresses Cas9-p2a-mcherry (BbsI site silenced) and sgRNA.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9279)
AUTHORS Gu B, Comerci CJ, McCarthy DG, Saurabh S, Moerner WE, Wysocka J
TITLE Opposing Effects of Cohesin and Transcription on CTCF Organization
Revealed by Super-resolution Imaging.
JOURNAL Mol Cell. 2020 Nov 19;80(4):699-711.e7. doi:
10.1016/j.molcel.2020.10.001. Epub 2020 Oct 21.
PUBMED 33091336
REFERENCE 2 (bases 1 to 9279)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 25, 2024 from SnapGene Server 7.0.3
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1016/j.molcel.2020.10.001"; journalName: "Mol Cell"; date:
"2020-11-19- 19"; volume: "80"; issue: "4"; pages: "699-711.e7"
FEATURES Location/Qualifiers
source 1..9279
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 1..249
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA
(Domitrovich & Kunkel, 2003)"
primer_bind 1..21
/label=hU6-F
/note="Human U6 promoter, forward primer"
primer_bind 172..191
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
misc_RNA 268..343
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
enhancer 440..725
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer;
contains an 18-bp deletion relative to the standard CMV
enhancer"
intron 1005..1232
/label=hybrid intron
/note="hybrid between chicken beta-actin (CBA) and minute
virus of mice (MMV) introns (Gray et al., 2011)"
regulatory 1244..1253
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 1253..1318
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
CDS 1325..1345
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 1370..5470
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 1370..5470
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 5471..5518
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
CDS 5528..5581
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 5588..6292
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
GRHSTGGMDELYK"
primer_bind complement(5730..5748)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind complement(6320..6337)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 6326..6533
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
repeat_region 6542..6682
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
repeat_region 6542..6671
/label=AAV2 ITR
rep_origin 6757..7212
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6844..6863)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 7054..7075
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(7229..7248)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 7348..7370
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(7408..7426)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 7494..7598
/gene="bla"
/label=AmpR promoter
CDS 7599..8459
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7817..7836)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 8630..9218
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 9119..9138
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
241 cgaaacaccg ggtcttcgag aagacctgtt ttagagctag aaatagcaag ttaaaataag
301 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttg ttttagagct
361 agaaatagca agttaaaata aggctagtcc gtttttagcg cgtgcgccaa ttctgcagac
421 aaatggctct agaggtaccc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
481 ccaacgaccc ccgcccattg acgtcaatag taacgccaat agggactttc cattgacgtc
541 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
601 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tgtgcccagt
661 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
721 ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac
781 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg
841 ggggggggsg sgcscsrggs ggggsggggs ggggsgrggg gsggggsggg gsgrggsggr
901 grggkgsggs ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc
961 ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct
1021 gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga
1081 ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc
1141 tgagcaagag gtaagggttt aagggatggt tggttggtgg ggtattaatg tttaattacc
1201 tggagcacct gcctgaaatc actttttttc aggttggacc ggtgccacca tggactataa
1261 ggaccacgac ggagactaca aggatcatga tattgattac aaagacgatg acgataagat
1321 ggccccaaag aagaagcgga aggtcggtat ccacggagtc ccagcagccg acaagaagta
1381 cagcatcggc ctggacatcg gcaccaactc tgtgggctgg gccgtgatca ccgacgagta
1441 caaggtgccc agcaagaaat tcaaggtgct gggcaacacc gaccggcaca gcatcaagaa
1501 gaacctgatc ggagccctgc tgttcgacag cggcgaaaca gccgaggcca cccggctgaa
1561 gagaaccgcc agaagaagat acaccagacg gaagaaccgg atctgctatc tgcaagagat
1621 cttcagcaac gagatggcca aggtggacga cagcttcttc cacagactgg aagagtcctt
1681 cctggtggaa gaggataaga agcacgagcg gcaccccatc ttcggcaaca tcgtggacga
1741 ggtggcctac cacgagaagt accccaccat ctaccacctg agaaagaaac tggtggacag
1801 caccgacaag gccgacctgc ggctgatcta tctggccctg gcccacatga tcaagttccg
1861 gggccacttc ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg acaagctgtt
1921 catccagctg gtgcagacct acaaccagct gttcgaggaa aaccccatca acgccagcgg
1981 cgtggacgcc aaggccatcc tgtctgccag actgagcaag agcagacggc tggaaaatct
2041 gatcgcccag ctgcccggcg agaagaagaa tggcctgttc ggaaacctga ttgccctgag
2101 cctgggcctg acccccaact tcaagagcaa cttcgacctg gccgaggatg ccaaactgca
2161 gctgagcaag gacacctacg acgacgacct ggacaacctg ctggcccaga tcggcgacca
2221 gtacgccgac ctgtttctgg ccgccaagaa cctgtccgac gccatcctgc tgagcgacat
2281 cctgagagtg aacaccgaga tcaccaaggc ccccctgagc gcctctatga tcaagagata
2341 cgacgagcac caccaggacc tgaccctgct gaaagctctc gtgcggcagc agctgcctga
2401 gaagtacaaa gagattttct tcgaccagag caagaacggc tacgccggct acattgacgg
2461 cggagccagc caggaagagt tctacaagtt catcaagccc atcctggaaa agatggacgg
2521 caccgaggaa ctgctcgtga agctgaacag agaggacctg ctgcggaagc agcggacctt
2581 cgacaacggc agcatccccc accagatcca cctgggagag ctgcacgcca ttctgcggcg
2641 gcaggaagat ttttacccat tcctgaagga caaccgggaa aagatcgaga agatcctgac
2701 cttccgcatc ccctactacg tgggccctct ggccagggga aacagcagat tcgcctggat
2761 gaccagaaag agcgaggaaa ccatcacccc ctggaacttc gaggaagtgg tggacaaggg
2821 cgcttccgcc cagagcttca tcgagcggat gaccaacttc gataagaacc tgcccaacga
2881 gaaggtgctg cccaagcaca gcctgctgta cgagtacttc accgtgtata acgagctgac
2941 caaagtgaaa tacgtgaccg agggaatgag aaagcccgcc ttcctgagcg gcgagcagaa
3001 aaaggccatc gtggacctgc tgttcaagac caaccggaaa gtgaccgtga agcagctgaa
3061 agaggactac ttcaagaaaa tcgagtgctt cgactccgtg gaaatctccg gcgtggaaga
3121 tcggttcaac gcctccctgg gcacatacca cgatctgctg aaaattatca aggacaagga
3181 cttcctggac aatgaggaaa acgaggacat tctggaagat atcgtgctga ccctgacact
3241 gtttgaggac agagagatga tcgaggaacg gctgaaaacc tatgcccacc tgttcgacga
3301 caaagtgatg aagcagctga agcggcggag atacaccggc tggggcaggc tgagccggaa
3361 gctgatcaac ggcatccggg acaagcagtc cggcaagaca atcctggatt tcctgaagtc
3421 cgacggcttc gccaacagaa acttcatgca gctgatccac gacgacagcc tgacctttaa
3481 agaggacatc cagaaagccc aggtgtccgg ccagggcgat agcctgcacg agcacattgc
3541 caatctggcc ggcagccccg ccattaagaa gggcatcctg cagacagtga aggtggtgga
3601 cgagctcgtg aaagtgatgg gccggcacaa gcccgagaac atcgtgatcg aaatggccag
3661 agagaaccag accacccaga agggacagaa gaacagccgc gagagaatga agcggatcga
3721 agagggcatc aaagagctgg gcagccagat cctgaaagaa caccccgtgg aaaacaccca
3781 gctgcagaac gagaagctgt acctgtacta cctgcagaat gggcgggata tgtacgtgga
3841 ccaggaactg gacatcaacc ggctgtccga ctacgatgtg gaccatatcg tgcctcagag
3901 ctttctgaag gacgactcca tcgacaacaa ggtgctgacc agaagcgaca agaaccgggg
3961 caagagcgac aacgtgccct ccgaagaggt cgtgaagaag atgaagaact actggcggca
4021 gctgctgaac gccaagctga ttacccagag aaagttcgac aatctgacca aggccgagag
4081 aggcggcctg agcgaactgg ataaggccgg cttcatcaag agacagctgg tggaaacccg
4141 gcagatcaca aagcacgtgg cacagatcct ggactcccgg atgaacacta agtacgacga
4201 gaatgacaag ctgatccggg aagtgaaagt gatcaccctg aagtccaagc tggtgtccga
4261 tttccggaag gatttccagt tttacaaagt gcgcgagatc aacaactacc accacgccca
4321 cgacgcctac ctgaacgccg tcgtgggaac cgccctgatc aaaaagtacc ctaagctgga
4381 aagcgagttc gtgtacggcg actacaaggt gtacgacgtg cggaagatga tcgccaagag
4441 cgagcaggaa atcggcaagg ctaccgccaa gtacttcttc tacagcaaca tcatgaactt
4501 tttcaagacc gagattaccc tggccaacgg cgagatccgg aagcggcctc tgatcgagac
4561 aaacggcgaa accggggaga tcgtgtggga taagggccgg gattttgcca ccgtgcggaa
4621 agtgctgagc atgccccaag tgaatatcgt gaaaaagacc gaggtgcaga caggcggctt
4681 cagcaaagag tctatcctgc ccaagaggaa cagcgataag ctgatcgcca gaaagaagga
4741 ctgggaccct aagaagtacg gcggcttcga cagccccacc gtggcctatt ctgtgctggt
4801 ggtggccaaa gtggaaaagg gcaagtccaa gaaactgaag agtgtgaaag agctgctggg
4861 gatcaccatc atggaaagaa gcagcttcga gaagaatccc atcgactttc tggaagccaa
4921 gggctacaaa gaagtgaaaa aggacctgat catcaagctg cctaagtact ccctgttcga
4981 gctggaaaac ggccggaaga gaatgctggc ctctgccggc gaactgcaga agggaaacga
5041 actggccctg ccctccaaat atgtgaactt cctgtacctg gccagccact atgagaagct
5101 gaagggctcc cccgaggata atgagcagaa acagctgttt gtggaacagc acaagcacta
5161 cctggacgag atcatcgagc agatcagcga gttctccaag agagtgatcc tggccgacgc
5221 taatctggac aaagtgctgt ccgcctacaa caagcaccgg gataagccca tcagagagca
5281 ggccgagaat atcatccacc tgtttaccct gaccaatctg ggagcccctg ccgccttcaa
5341 gtactttgac accaccatcg accggaagag gtacaccagc accaaagagg tgctggacgc
5401 caccctgatc caccagagca tcaccggcct gtacgagaca cggatcgacc tgtctcagct
5461 gggaggcgac aaaaggccgg cggccacgaa aaaggccggc caggcaaaaa agaaaaaggg
5521 cagtggagag ggcagaggaa gtctgctaac atgcggtgac gtcgaggaga atcctggccc
5581 agaattcgtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt
5641 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
5701 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct
5761 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa
5821 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga
5881 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
5941 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
6001 cgtaatgcag aagaaaacca tgggctggga ggcctcctcc gagcggatgt accccgagga
6061 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga
6121 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa
6181 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta
6241 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca aggaattcta
6301 actagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
6361 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
6421 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
6481 gggcaggaca gcaaggggga ggattgggaa gagaatagca ggcatgctgg ggagcggccg
6541 caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag
6601 gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag
6661 cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc
6721 ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa
6781 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccttagcgc
6841 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
6901 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
6961 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
7021 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
7081 cactcaactc tatctcgggc tattcttttg atttataagg gattttgccg atttcggtct
7141 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
7201 cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
7261 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
7321 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
7381 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
7441 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
7501 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
7561 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
7621 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
7681 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
7741 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
7801 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
7861 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
7921 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
7981 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
8041 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
8101 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
8161 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
8221 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
8281 ttattgctga taaatctgga gccggtgagc gtggaagccg cggtatcatt gcagcactgg
8341 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
8401 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
8461 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
8521 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
8581 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
8641 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
8701 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
8761 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
8821 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
8881 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
8941 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
9001 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
9061 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
9121 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
9181 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
9241 tacggttcct ggccttttgc tggccttttg ctcacatgt
//
PX458-mcherry载体图谱质粒图谱pdf版和PX458-mcherry载体序列质粒序列等相关资料下载
PX458-mcherry质粒载体应用举例
- 上一篇：pegCTNNB1 S45del
- 下一篇：pHDM-VSV-G
