| 载体名称: | pCWori |
|---|---|
| 质粒类型: | 原核表达载体 |
| 启动子: | 双tac/lac |
| 载体大小: | 5427 bp |
| 载体抗性: | 氨苄青霉素 |
| 备注: | NdeI与SpeI之间有400bp左右的填充序列，构建时需要删除 |
买家导航
pCWori载体质粒基本信息
pCWori质粒图谱载体图谱和pCWori载体序列质粒序列多克隆位点信息
pCWori质粒载体简介
pCWori质粒序列载体序列
LOCUS pCWori 5427 bp DNA circular SYN 05-MAR-2026
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5427)
AUTHORS DotmaticsRD
TITLE Direct Submission
JOURNAL Exported Mar 5, 2026 from SnapGene 8.1.0 to Vector NTI(R) format
https://www.biofeng.com
COMMENT VNTDATE|1122940800|
COMMENT VNTDBDATE|1122940800|
COMMENT VNTNAME|pCWori|
COMMENT VNTAUTHORNAME|DotmaticsRD|
FEATURES Location/Qualifiers
primer_bind complement(465..481)
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 662..1117
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1144..1248
/gene="bla"
/label=AmpR promoter
CDS join(1249..1317,1318..2109)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
rep_origin 2280..2868
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 3054..3196
/label=bom
/note="basis of mobility region from pBR322"
CDS complement(3317..3778)
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/label=lacZ-alpha
primer_bind 3742..3758
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3774..3790)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 3798..3814
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(join(3822..3828,3829..3846,3847..3852))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3867..3888
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
CDS complement(3901..4983)
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
promoter complement(4984..5061)
/gene="lacI (mutant)"
/label=lacIq promoter
/note="In the lacIq allele, a single base change in the
promoter boosts expression of the lacI gene about 10-fold."
promoter join(5149..5154,5155..5172,5173..5179)
/label=lac UV5 promoter
/note="E. coli lac promoter with an ""up"" mutation"
protein_bind 5187..5203
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter join(5258..5263,5264..5279,5280..5286)
/label=tac promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind 5294..5310
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter join(5353..5358,5359..5374,5375..5381)
/label=tac promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind 5389..5405
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
1 atcgatgctt aggaggtcat atggcggata aagaacttaa atttttggtt gtggatgact
61 tttccaccat gcgacgcata gtgcgtaacc tgctgaaaga gctgggattc aataatgttg
121 aggaagcgga agatggcgtc gacgctctca ataagttgca ggcaggcggt tatggatttg
181 ttatctccga ctggaacatg cccaatatgg atggcctgga attgctgaaa acaattcgtg
241 cggatggcgc gatgtcggca ttgccagtgt taatggtgac tgcagaagcg aagaaagaga
301 acatcattgc tgcggcgcaa gcgggggcca gtggctatgt ggtgaagcca tttaccgccg
361 cgacgctgga ggaaaaactc aacaaaatct ttgagaaact gggcatgtga ggatgcgact
421 atgatgcaac catcaatcaa acctgctgac gagcattcag gggggatcca ctagttctag
481 agtcgacctg cagcccaagc ttatcgatga taagctgtca aacatgagca gatctgagcc
541 cgcctaatga gcgggctttt ttttcagatc tgcttgaaga cgaaagggcc tcgtgatacg
601 cctattttta taggttaatg tcatgataat aatggtttct tagcgtcaaa gcaaccatag
661 tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
721 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
781 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
841 agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg
901 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
961 ggactcttgt tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta
1021 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
1081 aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat
1141 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
1201 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
1261 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
1321 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
1381 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
1441 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
1501 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
1561 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
1621 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
1681 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
1741 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg
1801 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
1861 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
1921 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
1981 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
2041 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
2101 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
2161 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
2221 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
2281 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
2341 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
2401 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
2461 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
2521 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
2581 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
2641 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
2701 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
2761 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
2821 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
2881 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
2941 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
3001 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
3061 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca
3121 gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga
3181 ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
3241 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
3301 gaggttttca ccgtcatcac cgaaacgcgc gaggcagaac gccatcaaaa ataattcgcg
3361 tctggccttc ctgtagccag ctttcatcaa cattaaatgt gagcgagtaa caacccgtcg
3421 gattctccgt gggaacaaac ggcggattga ccgtaatggg ataggttacg ttggtgtaga
3481 tgggcgcatc gtaaccgtgc atctgccagt ttgaggggac gacgacagta tcggcctcag
3541 gaagatcgca ctccagccag ctttccggca ccgcttctgg tgccggaaac caggcaaagc
3601 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
3661 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
3721 ggttttccca gtcacgacgt tgtaaaacga cggccagtga atccgtaatc atggtcatag
3781 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
3841 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
3901 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
3961 cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga
4021 gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc
4081 cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata
4141 acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag
4201 cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat
4261 cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc
4321 actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg
4381 ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat
4441 ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga
4501 gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt
4561 agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag
4621 cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct
4681 tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc
4741 cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa
4801 cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat
4861 cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg
4921 ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt
4981 cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt
5041 tttgcaccat tcgatggtgt cctggcacga caggtttccc gactggaaag cgggcagtga
5101 gcgcaacgca attaatgtaa gttagctcac tcattaggca ccccaggctt tacactttat
5161 gcttccggct cgtataatgt gtggaattgt gagcggataa caatttcaca caggaaacag
5221 gatcgatcca tcgatgagct tactccccat ccccctgttg acaattaatc atcggctcgt
5281 ataatgtgtg gaattgtgag cggataacaa tttcacacag gaaacaggat cagcttactc
5341 cccatccccc tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat
5401 aacaatttca cacaggaaac aggatcc
//
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