| 质粒名称: | pT2/HB |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 26557 |
| 存储实验室: | Perry Hackett |
|
载体骨架基本信息 |
|
| 载体名称: | pBluescript |
| 载体出品公司: | Stratagene |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 2918 bp |
| 载体类型: | 细菌表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | pT2/HB |
| 插入大小: | 3558 bp |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | HindIII(未破坏) |
| 3' 克隆位点: | BglII (未破坏) |
| 5' 测序引物 | T7 |
| 3' 测序引物 | T3 |
|
文献引用方法 |
|
| 材料和方法部分: | pT2/HB was a gift from Perry Hackett (Addgene plasmid # 26557) |
| 参考文献部分: | |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pT2/HB 3558 bp DNA SYN
DEFINITION pT2/HB
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..3558
/organism="pT2/HB"
/mol_type="other DNA"
misc_feature 34..56
/label="M13_pUC_fwd_primer"
promoter 49..65
/label="M13_forward20_primer"
promoter 75..93
/label="T7_promoter"
promoter complement(818..837)
/label="T3_promoter"
promoter complement(854..872)
/label="M13_reverse_primer"
misc_feature complement(871..893)
/label="M13_pUC_rev_primer"
promoter complement(907..936)
/label="lac_promoter"
gene complement(2019..2879)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(2019..2879)
/label="ORF frame 2"
promoter complement(2921..2949)
/label="AmpR_promoter"
rep_origin 3028..3334
/label="f1_origin"
misc_feature complement(3478..63)
/label="lacZ_a"
ORIGIN
1 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
61 ccagtgagcg cgcgtaatac gactcactat agggcgaatt ggagctcgga tccctataca
121 gttgaagtcg gaagtttaca tacacttaag ttggagtcat taaaactcgt ttttcaacta
181 ctccacaaat ttcttgttaa caaacaatag ttttggcaag tcagttagga catctacttt
241 gtgcatgaca caagtcattt ttccaacaat tgtttacaga cagattattt cacttataat
301 tcactgtatc acaattccag tgggtcagaa gtttacatac actaagttga ctgtgccttt
361 aaacagcttg gaaaattcca gaaaatgatg tcatggcttt agaagcttga tatccatgga
421 attcactagt gcgcgcggcc gctctagatg gccagatcta gcttgtggaa ggctactcga
481 aatgtttgac ccaagttaaa caatttaaag gcaatgctac caaatactaa ttgagtgtat
541 gtaaacttct gacccactgg gaatgtgatg aaagaaataa aagctgaaat gaatcattct
601 ctctactatt attctgatat ttcacattct taaaataaag tggtgatcct aactgaccta
661 agacagggaa tttttactag gattaaatgt caggaattgt gaaaaagtga gtttaaatgt
721 atttggctaa ggtgtatgta aacttccgac ttcaactgta tagggatcct ctagctagag
781 tcgacctcga gggggggccc ggtacccagc ttttgttccc tttagtgagg gttaattgcg
841 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
901 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
961 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
1021 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
1081 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
1141 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
1201 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
1261 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
1321 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
1381 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
1441 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
1501 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
1561 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
1621 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
1681 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
1741 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
1801 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
1861 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
1921 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
1981 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
2041 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
2101 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
2161 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
2221 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
2281 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
2341 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
2401 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
2461 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
2521 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
2581 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
2641 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
2701 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
2761 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
2821 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
2881 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
2941 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
3001 gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
3061 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
3121 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
3181 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
3241 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
3301 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
3361 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
3421 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttccat tcgccattca
3481 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
3541 cgaaaggggg atgtgctg
//
