pCas9

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联系方式:I47-825O-882O

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载体基本信息

出品公司: Addgene
载体名称: pCas9
质粒类型: Crisper/cas9 载体,用于敲除大肠杆菌基因
高拷贝/低拷贝: 低拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: p15a
载体大小: 9.3 kb
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: 氯霉素
克隆菌株: DH5α 等
宿主细胞(系): 常规大肠杆菌
备注: 用于常规大肠杆菌的基因crisper/cas9技术基因敲除
产品目录号: 42876
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pCas9载体图谱

pCas9载体特征


pCas9载体特征

载体简介

载体序列

LOCUS       pCas9                   9326 bp    DNA     circular SYN 12-DEC-2015
DEFINITION  Cloning vector pACYC184 backbone, pCas9 vector complete sequence.
ACCESSION   pCas9
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 9326)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     This file created by Clone Manager, Scientific & Educational Software
            http://www.biofeng.com/
COMMENT     VNTNAME|pCas9|
COMMENT     VNTOAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     misc_feature    581..1372
                     /gene="p15a"                    
                     /label=p15a
     misc_feature    complement(1844..2014)                    
                     /label=TracrRNA
                     /note="tracrRNA"
     CDS             2225..6331
                     /gene="cas9"
                     /transl_table=11                    
                     /label=cas9
     misc_feature    complement(6484..6519)                    
                     /label=Repeat
                     /note="Repeat"
     misc_feature    6520..6549
                     /gene="BsaI-spc"                  
                     /label=BsaI-spc
     misc_feature    6550..6585                     
                     /label=Repeat
                     /note="Repeat"
     CDS             6824..7852
                     /gene="truncated-tetAC"
                     /transl_table=11                   
                     /label=truncated-tetAC
     CDS             complement(8886..219)
                     /gene="cat" 
                     /label=Cm\Resistance     
BASE COUNT     2760 a      1915 c      2091 g      2560 t 
ORIGIN
        1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt 
       61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt 
      121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga 
      181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga 
      241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt 
      301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc 
      361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat 
      421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt 
      481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg 
      541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact 
      601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa 
      661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc 
      721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc 
      781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa 
      841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc 
      901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc 
      961 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc 
     1021 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac 
     1081 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt 
     1141 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt 
     1201 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg 
     1261 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt 
     1321 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc 
     1381 aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca 
     1441 atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc 
     1501 atgtttgaca gcttatcatc gataagcttt aatgcggtag tttatcacag ttaaattgct 
     1561 aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg 
     1621 tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg 
     1681 attacgaaat catcctgtgg agcttagtag gtttagcaag atggcagcgc ctaaatgtag 
     1741 aatgataaaa ggattaagag attaatttcc ctaaaaatga taaaacaagc gttttgaaag 
     1801 cgcttgtttt tttggtttgc agtcagagta gaatagaagt atcaaaaaaa gcaccgactc 
     1861 ggtgccactt tttcaagttg ataacggact agccttattt taacttgcta tgctgttttg 
     1921 aatggttcca acaagattat tttataactt ttataacaaa taatcaagga gaaattcaaa 
     1981 gaaatttatc agccataaaa caatacttaa tactatagaa tgataacaaa ataaactact 
     2041 ttttaaaaga attttgtgtt ataatctatt tattattaag tattgggtaa tattttttga 
     2101 agagatattt tgaaaaagaa aaattaaagc atattaaact aatttcggag gtcattaaaa 
     2161 ctattattga aatcatcaaa ctcattatgg atttaattta aactttttat tttaggaggc 
     2221 aaaaatggat aagaaatact caataggctt agatatcggc acaaatagcg tcggatgggc 
     2281 ggtgatcact gatgaatata aggttccgtc taaaaagttc aaggttctgg gaaatacaga 
     2341 ccgccacagt atcaaaaaaa atcttatagg ggctctttta tttgacagtg gagagacagc 
     2401 ggaagcgact cgtctcaaac ggacagctcg tagaaggtat acacgtcgga agaatcgtat 
     2461 ttgttatcta caggagattt tttcaaatga gatggcgaaa gtagatgata gtttctttca 
     2521 tcgacttgaa gagtcttttt tggtggaaga agacaagaag catgaacgtc atcctatttt 
     2581 tggaaatata gtagatgaag ttgcttatca tgagaaatat ccaactatct atcatctgcg 
     2641 aaaaaaattg gtagattcta ctgataaagc ggatttgcgc ttaatctatt tggccttagc 
     2701 gcatatgatt aagtttcgtg gtcatttttt gattgaggga gatttaaatc ctgataatag 
     2761 tgatgtggac aaactattta tccagttggt acaaacctac aatcaattat ttgaagaaaa 
     2821 ccctattaac gcaagtggag tagatgctaa agcgattctt tctgcacgat tgagtaaatc 
     2881 aagacgatta gaaaatctca ttgctcagct ccccggtgag aagaaaaatg gcttatttgg 
     2941 gaatctcatt gctttgtcat tgggtttgac ccctaatttt aaatcaaatt ttgatttggc 
     3001 agaagatgct aaattacagc tttcaaaaga tacttacgat gatgatttag ataatttatt 
     3061 ggcgcaaatt ggagatcaat atgctgattt gtttttggca gctaagaatt tatcagatgc 
     3121 tattttactt tcagatatcc taagagtaaa tactgaaata actaaggctc ccctatcagc 
     3181 ttcaatgatt aaacgctacg atgaacatca tcaagacttg actcttttaa aagctttagt 
     3241 tcgacaacaa cttccagaaa agtataaaga aatctttttt gatcaatcaa aaaacggata 
     3301 tgcaggttat attgatgggg gagctagcca agaagaattt tataaattta tcaaaccaat 
     3361 tttagaaaaa atggatggta ctgaggaatt attggtgaaa ctaaatcgtg aagatttgct 
     3421 gcgcaagcaa cggacctttg acaacggctc tattccccat caaattcact tgggtgagct 
     3481 gcatgctatt ttgagaagac aagaagactt ttatccattt ttaaaagaca atcgtgagaa 
     3541 gattgaaaaa atcttgactt ttcgaattcc ttattatgtt ggtccattgg cgcgtggcaa 
     3601 tagtcgtttt gcatggatga ctcggaagtc tgaagaaaca attaccccat ggaattttga 
     3661 agaagttgtc gataaaggtg cttcagctca atcatttatt gaacgcatga caaactttga 
     3721 taaaaatctt ccaaatgaaa aagtactacc aaaacatagt ttgctttatg agtattttac 
     3781 ggtttataac gaattgacaa aggtcaaata tgttactgaa ggaatgcgaa aaccagcatt 
     3841 tctttcaggt gaacagaaga aagccattgt tgatttactc ttcaaaacaa atcgaaaagt 
     3901 aaccgttaag caattaaaag aagattattt caaaaaaata gaatgttttg atagtgttga 
     3961 aatttcagga gttgaagata gatttaatgc ttcattaggt acctaccatg atttgctaaa 
     4021 aattattaaa gataaagatt ttttggataa tgaagaaaat gaagatatct tagaggatat 
     4081 tgttttaaca ttgaccttat ttgaagatag ggagatgatt gaggaaagac ttaaaacata 
     4141 tgctcacctc tttgatgata aggtgatgaa acagcttaaa cgtcgccgtt atactggttg 
     4201 gggacgtttg tctcgaaaat tgattaatgg tattagggat aagcaatctg gcaaaacaat 
     4261 attagatttt ttgaaatcag atggttttgc caatcgcaat tttatgcagc tgatccatga 
     4321 tgatagtttg acatttaaag aagacattca aaaagcacaa gtgtctggac aaggcgatag 
     4381 tttacatgaa catattgcaa atttagctgg tagccctgct attaaaaaag gtattttaca 
     4441 gactgtaaaa gttgttgatg aattggtcaa agtaatgggg cggcataagc cagaaaatat 
     4501 cgttattgaa atggcacgtg aaaatcagac aactcaaaag ggccagaaaa attcgcgaga 
     4561 gcgtatgaaa cgaatcgaag aaggtatcaa agaattagga agtcagattc ttaaagagca 
     4621 tcctgttgaa aatactcaat tgcaaaatga aaagctctat ctctattatc tccaaaatgg 
     4681 aagagacatg tatgtggacc aagaattaga tattaatcgt ttaagtgatt atgatgtcga 
     4741 tcacattgtt ccacaaagtt tccttaaaga cgattcaata gacaataagg tcttaacgcg 
     4801 ttctgataaa aatcgtggta aatcggataa cgttccaagt gaagaagtag tcaaaaagat 
     4861 gaaaaactat tggagacaac ttctaaacgc caagttaatc actcaacgta agtttgataa 
     4921 tttaacgaaa gctgaacgtg gaggtttgag tgaacttgat aaagctggtt ttatcaaacg 
     4981 ccaattggtt gaaactcgcc aaatcactaa gcatgtggca caaattttgg atagtcgcat 
     5041 gaatactaaa tacgatgaaa atgataaact tattcgagag gttaaagtga ttaccttaaa 
     5101 atctaaatta gtttctgact tccgaaaaga tttccaattc tataaagtac gtgagattaa 
     5161 caattaccat catgcccatg atgcgtatct aaatgccgtc gttggaactg ctttgattaa 
     5221 gaaatatcca aaacttgaat cggagtttgt ctatggtgat tataaagttt atgatgttcg 
     5281 taaaatgatt gctaagtctg agcaagaaat aggcaaagca accgcaaaat atttctttta 
     5341 ctctaatatc atgaacttct tcaaaacaga aattacactt gcaaatggag agattcgcaa 
     5401 acgccctcta atcgaaacta atggggaaac tggagaaatt gtctgggata aagggcgaga 
     5461 ttttgccaca gtgcgcaaag tattgtccat gccccaagtc aatattgtca agaaaacaga 
     5521 agtacagaca ggcggattct ccaaggagtc aattttacca aaaagaaatt cggacaagct 
     5581 tattgctcgt aaaaaagact gggatccaaa aaaatatggt ggttttgata gtccaacggt 
     5641 agcttattca gtcctagtgg ttgctaaggt ggaaaaaggg aaatcgaaga agttaaaatc 
     5701 cgttaaagag ttactaggga tcacaattat ggaaagaagt tcctttgaaa aaaatccgat 
     5761 tgacttttta gaagctaaag gatataagga agttaaaaaa gacttaatca ttaaactacc 
     5821 taaatatagt ctttttgagt tagaaaacgg tcgtaaacgg atgctggcta gtgccggaga 
     5881 attacaaaaa ggaaatgagc tggctctgcc aagcaaatat gtgaattttt tatatttagc 
     5941 tagtcattat gaaaagttga agggtagtcc agaagataac gaacaaaaac aattgtttgt 
     6001 ggagcagcat aagcattatt tagatgagat tattgagcaa atcagtgaat tttctaagcg 
     6061 tgttatttta gcagatgcca atttagataa agttcttagt gcatataaca aacatagaga 
     6121 caaaccaata cgtgaacaag cagaaaatat tattcattta tttacgttga cgaatcttgg 
     6181 agctcccgct gcttttaaat attttgatac aacaattgat cgtaaacgat atacgtctac 
     6241 aaaagaagtt ttagatgcca ctcttatcca tcaatccatc actggtcttt atgaaacacg 
     6301 cattgatttg agtcagctag gaggtgactg aagtatattt tagatgaaga ttatttctta 
     6361 ataactaaaa atatggtata atactcttaa taaatgcagt aatacagggg cttttcaaga 
     6421 ctgaagtcta gctgagacaa atagtgcgat tacgaaattt tttagacaaa aatagtctac 
     6481 gaggttttag agctatgctg ttttgaatgg tcccaaaact gagaccagtc tcggaagctc 
     6541 aaaggtctcg ttttagagct atgctgtttt gaatggtccc aaaacttcag cacactgaga 
     6601 cttgttgagt tccatgtttt agagctatgc tgttttgaat ggactccatt caacattgcc 
     6661 gatgataact tgagaaagag ggttaatacc agcagtcgga taccttccta ttctttctgt 
     6721 taaagcgttt tcatgttata ataggcaaaa gaagagtagt gtgatcgtcc attccgacag 
     6781 catcgccagt cactatggcg tgctgctagc gctatatgcg ttgatgcaat ttctatgcgc 
     6841 acccgttctc ggagcactgt ccgaccgctt tggccgccgc ccagtcctgc tcgcttcgct 
     6901 acttggagcc actatcgact acgcgatcat ggcgaccaca cccgtcctgt ggatcctcta 
     6961 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat 
     7021 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt 
     7081 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca 
     7141 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct 
     7201 aatgcaggag tcgcataagg gagagcgtcg accgatgccc ttgagagcct tcaacccagt 
     7261 cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt 
     7321 tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg 
     7381 ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc 
     7441 cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga agcaggccat 
     7501 tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg 
     7561 ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga tgcccgcgtt 
     7621 gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc aaggatcgct 
     7681 cgcggctctt accagcctaa cttcgatcat tggaccgctg atcgtcacgg cgatttatgc 
     7741 cgcctcggcg agcacatgga acgggttggc atggattgta ggcgccgccc tataccttgt 
     7801 ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct gaatggaagc 
     7861 cggcggcacc tcgctaacgg attcaccact ccaagaattg gagccaatca attcttgcgg 
     7921 agaactgtga atgcgcaaac caacccttgg cagaacatat ccatcgcgtc cgccatctcc 
     7981 agcagccgca cgcggcgcat ctcgggcagc gttgggtcct ggccacgggt gcgcatgatc 
     8041 gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat 
     8101 gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga 
     8161 gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtcc 
     8221 cctacgtgct gctgaagttg cccgcaacag agagtggaac caaccggtga taccacgata 
     8281 ctatgactga gagtcaacgc catgagcggc ctcatttctt attctgagtt acaacagtcc 
     8341 gcaccgctgt ccggtagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt 
     8401 atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgcc caacagtccc 
     8461 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgccctgcac cattatgttc 
     8521 cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc tgtattaacg 
     8581 aagcgctaac cgtttttatc aggctctggg aggcagaata aatgatcata tcgtcaatta 
     8641 ttacctccac ggggagagcc tgagcaaact ggcctcaggc atttgagaag cacacggtca 
     8701 cactgcttcc ggtagtcaat aaaccggtaa accagcaata gacataagcg gctatttaac 
     8761 gaccctgccc tgaaccgacg accgggtcga atttgctttc gaatttctgc cattcatccg 
     8821 cttattatca cttattcagg cgtagcacca ggcgtttaag ggcaccaata actgccttaa 
     8881 aaaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg 
     8941 ccgacatgga agccatcaca gacggcatga tgaacctgaa tcgccagcgg catcagcacc 
     9001 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata 
     9061 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac 
     9121 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct 
     9181 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa 
     9241 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc 
     9301 agctcaccgt ctttcattgc catacg 
//

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