aMHC-eGFP-Rex-Neo

价格:1200元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

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载体基本信息

质粒名称: aMHC-eGFP-Rex-Neo 
出品公司: 爱迪基因
目录编号: 21229
存储实验室: Mark Mercola

载体骨架基本信息
载体名称: SIN18
载体抗性: 氨苄霉素
空载体大小: 8400 bp
载体类型: 慢病毒载体
筛选标记: 新霉素抗性标记Rex-1基因启动子驱动
高拷贝/低拷贝: 未知
生长条件: 37 ℃

插入基因信息
插入基因名称: alpha myosin heavy chain promoter
物种来源: M.肌肉(老鼠)
插入大小: 5470 bp
标签/融合蛋白: eGFP(C端)
克隆方法: 限制性内切酶
5' 克隆位点: claI(未破坏)
3' 克隆位点: AgeI (未破坏)
5' 测序引物 CTTGATGCCGTTCTTCTGCTT - 3' Sequencing 

文献引用方法
材料和方法部分: aMHC-eGFP-Rex-Neo was a gift from Mark Mercola (Addgene plasmid # 21229)
参考文献部分: Lentiviral vectors and protocols for creation of stable hESC lines for fluorescent tracking and drug resistance selection of cardiomyocytes. Kita-Matsuo H, Barcova M, Prigozhina N, Salomonis N, Wei K, Jacot JG, Nelson B, Spiering S, Haverslag R, Kim C, Talantova M, Bajpai R, Calzolari D, Terskikh A, McCulloch AD, Price JH, Conklin BR, Chen HS, Mercola M. PLoS ONE. 2009 . 4(4):e5046. 10.1371/journal.pone.0005046 PubMed 19352491

载体图谱质粒图谱和多克隆位点信息

aMHC-eGFP-Rex-Neo载体图谱

载体简介

载体序列

LOCUS       aMHC-eGFP-Rex-Neo	13870 bp 	DNA	SYN
DEFINITION  aMHC-eGFP-Rex-Neo
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..13870
                     /organism="aMHC-eGFP-Rex-Neo"
                     /mol_type="other DNA"
     promoter        62..90
                     /label="AmpR_promoter"
     gene            132..992
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             132..992
                     /label="ORF frame 3"
     rep_origin      1147..1766
                     /label="pBR322_origin"
     promoter        2075..2104
                     /label="lac_promoter"
     misc_feature    2118..2140
                     /label="M13_pUC_rev_primer"
     promoter        2139..2157
                     /label="M13_reverse_primer"
     promoter        2174..2193
                     /label="T3_promoter"
     misc_feature    2448..2628
                     /label="truncHIV-1_3_LTR"
     misc_feature    2448..2628
                     /label="HIV-1_5_LTR"
     misc_feature    2736..2780
                     /label="HIV-1_psi_pack"
     CDS             3168..4331
                     /label="ORF frame 3"
     misc_feature    3290..3523
                     /label="RRE"
     misc_feature    4023..4038
                     /label="cPPT"
     misc_feature    4181..4197
                     /label="pBluescriptSK_primer"
     CDS             complement(8677..9189)
                     /label="ORF frame 2"
     CDS             9670..10389
                     /label="ORF frame 1"
     gene            9673..10386
                     /label="EGFP"
                     /gene="EGFP"
     misc_feature    complement(9715..9736)
                     /label="EGFP_N_primer"
     misc_feature    9853..9882
                     /label="Y66 (EGFP)"
     misc_feature    10323..10344
                     /label="EGFP_C_primer"
     misc_feature    10407..10982
                     /label="WPRE"
     CDS             10495..11031
                     /label="ORF frame 1"
     gene            11735..12535
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
     CDS             11735..12538
                     /label="ORF frame 2"
     misc_feature    12594..12609
                     /label="cPPT"
     misc_feature    12594..12615
                     /label="U3PPT"
     misc_feature    12611..12663
                     /label="delta_U3"
     misc_feature    12664..12844
                     /label="truncHIV-1_3_LTR"
     misc_feature    12664..12844
                     /label="HIV-1_5_LTR"
     terminator      12919..13038
                     /label="SV40_PA_terminator"
     misc_feature    13007..13026
                     /label="EBV_rev_primer"
     rep_origin      13056..13133
                     /label="SV40_origin"
     misc_feature    13118..13137
                     /label="SV40pro_F_primer"
     promoter        complement(13224..13242)
                     /label="T7_promoter"
     promoter        complement(13252..13268)
                     /label="M13_forward20_primer"
     misc_feature    complement(13261..13283)
                     /label="M13_pUC_fwd_primer"
     misc_feature    13261..13404
                     /label="lacZ_a"
     rep_origin      13430..13736
                     /label="f1_origin"
ORIGIN
    1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
   61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
  121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
  181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
  241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
  301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
  361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
  421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
  481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
  541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
  601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
  661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
  721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
  781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
  841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
  901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
  961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
 1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
 1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
 1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
 1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
 1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
 1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
 1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
 1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
 1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
 1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
 1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
 1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
 1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
 1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
 1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
 1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
 1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
 2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
 2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
 2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
 2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
 2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
 2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
 2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
 2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
 2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
 2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
 2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
 2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
 2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
 2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
 2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
 2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
 3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
 3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
 3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
 3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
 3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
 3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
 3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
 3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
 3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
 3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
 3601 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
 3661 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
 3721 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
 3781 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
 3841 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
 3901 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
 3961 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt
 4021 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
 4081 caacagacat acaaacaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
 4141 tcacgagact agcctcgagc tccaccgcgg tggcggccgc tctagaacta gtggatcctg
 4201 caaggtcaca caagggtctc cacccaccag gtgccctagt ctcaatttca gtttccatgc
 4261 cttgttctca caatgctggc ctccccagag ctaatttgga ctttgttttt atttcaaaag
 4321 ggcctgaatg aggagtagat cttgtgctac ccagctctaa gggtgcccgt gaagccctca
 4381 gacctggagc ctttgcaaca gccctttagg tggaagcaga ataaagcaat tttccttaaa
 4441 gccaaaatcc tgcctctaga ctcttcttct ctgacctcgg tccctgggct ctagggtggg
 4501 gaggtggggc ttggaagaag aaggtgggga agtggcaaaa gccgatccct agggccctgt
 4561 gaagttcgga gccttccctg tacagcactg gctcatagat cctcctccag ccaaacatag
 4621 caagaagtga tacctccttt gtgacttccc caggcccagt acctgtcagg ttgaaacagg
 4681 atttagagaa gcctctgaac tcacctgaac tctgaagctc atccaccaag caagcaccta
 4741 ggtgccactg ctagttagta tcctacgctg ataatatgca gagctgggcc acagaagtcc
 4801 tggggtgtag gaactgacca gtgacttttc agtcggcaaa ggtatgaccc cctcagcaga
 4861 tgtagtaatg tccccttaga tcccatccca ggcaggtctc taagaggaca tgggatgaga
 4921 gatgtagtca tgtggcattc caaacacagc tatccacagt gtcccttgcc ccttccactt
 4981 agccaggagg acagtaacct tagcctatct ttcttcctcc ccatcctccc aggacacacc
 5041 ccctggtctg cagtattcat ttcttccttc acgtcccctc tgtgacttcc atttgcaagg
 5101 cttttgacct ctgcagctgc tggaagatag agtttggccc taggtgtggc aagccatctc
 5161 aagagaaagc agacaacagg gggaccagat tttggaagga tcaggaacta aatcactggc
 5221 gggcctgggg gtagaaaaaa gagtgagtga gtccgctcca gctaagccaa gctagtcccc
 5281 gagatactct gccacagctg ggctgctcgg ggtagcttta ggaatgtggg tctgaaagac
 5341 aatgggattg gaagacatct ctttgagtct cccctcaacc ccacctacag acacactcgt
 5401 gtgtggccag actcctgttc aacagccctc tgtgttctga ccactgagct aggcaaccag
 5461 agcatgggcc ctgtgctgag gatgaagagt tggttaccaa tagcaaaaac agcaggggag
 5521 ggagaacaga gaacgaaata aggaaggaag aaggaaaggc cagtcaatca gatgcagtca
 5581 gaagagatgg gaagccaaca cacagcttga gcagaggaaa cagaaaaggg agagattctg
 5641 ggcataagga ggccacagaa agaagagccc aggcccccca agtctcctct ttataccctc
 5701 atcccgtctc ccaattaagc ccactcttct tcctagatca gacctgagct gcagcgaaga
 5761 gacccgtagg gaggatcaca ctggatgaag gagatgtgtg gagaagtcca gggcaaccta
 5821 agagccagag cctaaaagag caagagataa aggtgcttca aaggtggcca ggctgtgcac
 5881 acagagggtc gaggactggt ggtagagcct caagataagg atgatgctca gaatgggcgg
 5941 ggggggggat tctggggggg ggagagagaa ggtgagaagg agcctggaac agagaatctg
 6001 gaagcgctgg aaacgatacc ataaagggaa gaacccaggc tacctttaga tgtaaatcat
 6061 gaaagacagg gagaagggaa gctggagaga gtagaaggac cccggggcaa gacatggaag
 6121 caaggacaag ccaggttgag cgctccgtga aatcagcctg ctgaaggcag agccctggta
 6181 tgagcaccag aacagcagag gctagggtta atgtcgagac agggaacaga aggtagacac
 6241 aggaacagac agagacgggg gagccaggta acaaaggaat ggtccttctc acctgtggcc
 6301 agagcgtcca tctgtgtcca catactctag aatgttcatc agactgcagg gctggcttgg
 6361 gaggcagctg gaaagagtat gtgagagcca ggggagacaa gggggcctag gaaaggaaga
 6421 agagggcaaa ccaggccaca caagagggca gagcccagaa ctgagttaac tccttccttg
 6481 ttgcatcttc cataggaggc agtgggaact ctgtgaccac catcccccat gagcccccac
 6541 tacccatacc aagtttggcc tgagtggcat tctaggttcc ctgaggacag agcctggcct
 6601 ttgtctcttg gacctgaccc aagctgaccc aatgttctca gtaccttatc atgccctcaa
 6661 gagcttgaga accaggcagt gacatattag gccatgggct aaccctggag cttgcacaca
 6721 ggagcctcaa gtgacctcca gggacacagc tgcagacagg tggcctttat ccccaaagag
 6781 caaccatttg gcataggtgg ctgcaaatgg gaatgcaagg ttgaatcagg tcccttcaag
 6841 aatactgcat gcaagaccta agacccctgg agagaggggt atgctcctgc ccccacccac
 6901 cataagggga gtgaactatc ctagggggct ggcgaccttg gggagacacc acattactga
 6961 gagtgctgag cccagaaaaa ctgaccgccc tgtgtcctgc ccacctccac actctagagc
 7021 tatattgaga ggtgacagta gatagggtgg gagctggtag cagggagagt gttcctgggt
 7081 gtgagggtgt aggggaaagc cagagcaggg gagtctggct ttgtctcctg aacacaatgt
 7141 ctacttagtt ataacaggca tgacctgcta aagacccaac atctacgacc tctgaaaaga
 7201 cagcagccct ggaggacagg ggttgtctct gagccttggg tgcttgatgg tgccacaaag
 7261 gagggcatga gtgtgagtat aaggccccag gagcgttaga gaagggcact tgggaagggg
 7321 tcagtctgca gagcccctat ccatggaatc tggagcctgg ggccaactgg tgtaaatctc
 7381 tgggcctgcc aggcattcaa agcagcacct gcatcctctg gcagcctggg gaggcggaag
 7441 ggagcaaccc cccacttata ccctttctcc ctcagcccca ggattaacac ctctggcctt
 7501 cccccttccc acctcccatc aggagtggag ggttgcagag ggagggtaaa aacctacatg
 7561 tccaaacatc atggtgcacg atatatggat cagtatgtgt agaggcaaga aaggaaatct
 7621 gcaggcttaa ctgggttaat gtgtaaagtc tgtgtgcatg tgtgtgtgtc tgactgaaaa
 7681 cgggcatggc tgtgcagctg ttcagttctg tgcgtgaggt taccagactg caggtttgtg
 7741 tgtaaattgc ccaaggcaaa gtgggtgaat cccttccatg gtttaaagag attggatgat
 7801 ggcctgcatc tcaaggacca tggaaaatag aatggacact ctatatgtgt ctctaagcta
 7861 aggtagcaag gtctttggag gacacctgtc tagagatgtg ggcaacagag actacagaca
 7921 gtatctgtac agagtaagga gagagaggag ggggtgtaga attctcttac tatcaaaggg
 7981 aaactgagtc gtgcacctgc aaagtggatg ctctccctag acatcatgac tttgtctctg
 8041 gggagccagc actgtggaac ttcaggtctg agagagtagg aggctcccct cagcctgaag
 8101 ctatgcagat agccagggtt gaaaggggga agggagagcc tgggatggga gcttgtgtgt
 8161 tggaggcagg ggacagatat taagcctgga agagaaggtg acccttaccc agttgttcaa
 8221 ctcacccttc agattaaaaa taactgaggt aagggcctgg gtaggggagg tggtgtgaga
 8281 cgctcctgtc tctcctctat ctgcccatcg gccctttggg gaggaggaat gtgcccaagg
 8341 actaaaaaaa ggccatggag ccagaggggc gagggcaaca gacctttcat gggcaaacct
 8401 tggggccctg ctgtcctcct gtcacctcca gagccaaggg atcaaaggag gaggagccag
 8461 gacaggaggg aagtgggagg gagggtccca gcagaggact ccaaatttag gcagcaggca
 8521 tatgggatgg gatataaagg ggctggagca ctgagagctg tcagagattt ctccaaccca
 8581 ggtaagaggg agtttcgggt gggggctctt cacccacacc agacctctcc ccacctagaa
 8641 ggaaactgcc tttcctggaa gtggggttca ggccggtcag agatctgaca gggtggcctt
 8701 ccaccagcct gggaagttct cagtggcagg aggtttccac aagaaacact ggatgcccct
 8761 tcccttacgc tgtcttctcc atcttcctcc tggggatgct cctccccgtc ttggtttatc
 8821 ttggctcttc gtcttcagca agatttgccc tgtgctgtcc actccatctt tctctactgt
 8881 ctccgtgcct tgccttgcct tcttgcgtgt ccttcctttc cacccatttc tcacttcacc
 8941 ttttctcccc ttctcatttg tattcatcct tccttccttc cttccttcct tccttccttc
 9001 cttccttcct tcctttctcc cttccttcct tccttccttc cttccttcct tccttccttc
 9061 ctgtgtcaga gtgctgagaa tcacacctgg ggttcccacc cttatgtaaa caatcttcca
 9121 gtgagccaca gcttcagtgc tgctgggtgc tctcttacct tcctcacccc ctggcttgtc
 9181 ctgttccatc ctggtcagga tctctagatt ggtctcccag cctctgctac tcctcttcct
 9241 gcctgttcct ctctctgtcc agctgcgcca ctgtggtgcc tcgttccagc tgtggtccac
 9301 attcttcagg attctctgaa aagttaacca ggtgagaatg tttcccctgt agacagcaga
 9361 tcacgattct cccggaagtc aggcttccag ccctctcttt ctctgcccag ctgcccggca
 9421 ctcttagcaa acctcaggca cccttacccc acatagacct ctgacagaga agcaggcact
 9481 ttacatggag tcctggtggg agagccatag gctacggtgt aaaagaggca gggaagtggt
 9541 ggtgtaggaa agtcaggact tcacatagaa gcctagccca caccagaaat gacagacaga
 9601 tccctcctat ctcccccata agagtttgag tcgacggtac cgcgggcccg ggatccaccg
 9661 gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
 9721 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
 9781 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
 9841 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
 9901 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
 9961 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
10021 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
10081 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
10141 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
10201 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
10261 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
10321 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
10381 tacaagtaaa gcggccgcgt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
10441 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
10501 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
10561 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
10621 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
10681 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
10741 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
10801 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
10861 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
10921 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
10981 gccgcctccc cgcctgaatt tcaccggcga ccgattcctc ccgataagta aattacagaa
11041 aagcttcagg ttaaatgtaa gaggatctta aatattgatt tggtttttgt cttaatggtg
11101 cccggcgtat ttataagaac cattaaattt cactagaccg tttgtagtca ggggtgaacg
11161 ctgcatgacc cacgctctca aagtgagttg tgttgctttg cgggaatcca gcagtcacat
11221 taatgctgga aaaagttcag gcaactagtg tactttgtag cggggtccgg gagaggctgg
11281 ggtctagagt ggcgatggga cgaaagggta aagaaagttt tcggtgtgtg tgtgtgtgtg
11341 tgtgtgtgtg tgtgtgtgtg tgtgtgtgta ggtaggtagg taggtagaaa ctgcatcctc
11401 tgcttgtgta aatccggtta catgtgtgta acagaggtac tgagatgtga ctgagtctca
11461 aggccaggcg atcgggattc agaagaggca tttgcataac tgagcaagag cctttgcccc
11521 acccttccac gcggacccaa gacaagcgga cagctctggg tgggtcacct tgaagccagg
11581 ggcccgccca catccccgcc cacacccacc ttgagcgctt ctcattgggc attccagcct
11641 acccagctcg gagttagtta ctccgtaagt gtggccggaa cagagttcgt ccatctaaaa
11701 agggagggga ccggcgaaca catctagagc caccatggga tcggccattg aacaagatgg
11761 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
11821 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
11881 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
11941 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
12001 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
12061 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
12121 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
12181 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
12241 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgatg atctcgtcgt
12301 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
12361 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
12421 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
12481 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgaag
12541 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
12601 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
12661 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
12721 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
12781 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
12841 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
12901 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
12961 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
13021 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt
13081 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
13141 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
13201 gcgtcgagac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
13261 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
13321 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
13381 acagttgcgc agcctgaatg gcgaatggcg cgacgcgccc tgtagcggcg cattaagcgc
13441 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
13501 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
13561 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
13621 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
13681 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
13741 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
13801 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
13861 tacaatttcc
//

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