pLVX-Het-1

价格:3500元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pLVX-Het-1
质粒类型: 慢病毒载体;双顺反子载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: CMV
载体大小: 8437 bp
5' 测序引物及序列: CMV Forward: CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列: --
载体标签: DmrC (C、N-端均可)
载体抗性: 氨苄青霉素
筛选标记: 嘌呤霉素(Puromycin)
克隆菌株: Stbl3
宿主细胞(系): 常规细胞系(293、CV-1、CHO等)
备注: 慢病毒载体pLVX-Het-1组成型表达DmrC融合蛋白;
外源基因与嘌呤霉素抗性基因以双顺反子的形式表达。
产品目录号: 635074
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLVX-Het-1载体图谱



pLVX-Het-1 多克隆位点

pLVX-Het-1 载体特征

载体简介

载体序列

LOCUS       pLVX-Het1    8437 bp    DNA   circular             8-NOV-2011
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No.635074, 635075  
FEATURES             Location/Qualifiers
     LTR             1..635
                     /label=5'\LTR
     misc_feature    685..822
                     /label=packaging\signal
     misc_feature    2028..2151
                     /label=CTS/cppt\(DNA\flap)
     promoter        2185..2788
                     /label=pCMV
     misc_feature    4359..4950
                     /label=WPRE
     rep_origin      complement(6260..6930)
                     /label=pUC\origin
     CDS             complement(7075..8071)
                     /label=Amp(R)
     misc_binding    1303..1536
                     /label=RRE
     LTR             5154..5790
                     /label=3'\LTR
     misc_feature    3138..3713
                     /label=IRES
     CDS             3746..4345
                     /label=Puro(R)
     misc_feature    2822..3121
                     /label=DmrC
BASE COUNT     2162 a      2086 c      2179 g      2010 t 
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc 
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 
     2521 agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 
     2761 tccatagaag acaccgactc tagctagagg atctatttcc ggtgaattcc tcgagactag 
     2821 tatggcttct agaatcctct ggcatgagat gtggcatgaa ggcctggaag aggcatctcg 
     2881 tttgtacttt ggggaaagga acgtgaaagg catgtttgag gtgctggagc ccttgcatgc 
     2941 tatgatggaa cggggccccc agactctgaa ggaaacatcc tttaatcagg cctatggtcg 
     3001 agatttaatg gaggcccaag agtggtgcag gaagtacatg aaatcaggga atgtcaagga 
     3061 cctcctccaa gcctgggacc tctattatca tgtgttccga cgaatctcaa agagcggccg 
     3121 cggatcctac gtatgaccgc ccctctccct cccccccccc taacgttact ggccgaagcc 
     3181 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt 
     3241 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc 
     3301 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc 
     3361 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc 
     3421 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg 
     3481 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct 
     3541 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat 
     3601 ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc 
     3661 taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga taagcttgcc 
     3721 acaacccaca aggagacgac cttccatgac cgagtacaag cccacggtgc gcctcgccac 
     3781 ccgcgacgac gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc 
     3841 cacgcgccac accgtcgacc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact 
     3901 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc 
     3961 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg 
     4021 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct 
     4081 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc 
     4141 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga 
     4201 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga 
     4261 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg 
     4321 catgacccgc aagcccggtg cctagacgcg tctggaacaa tcaacctctg gattacaaaa 
     4381 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg 
     4441 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct 
     4501 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg 
     4561 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct 
     4621 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg 
     4681 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg 
     4741 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc 
     4801 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc 
     4861 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc 
     4921 ggatctccct ttgggccgcc tccccgcctg gaattaattc tgcagtcgag acctagaaaa 
     4981 acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga 
     5041 agcacaagag gaggaggagg tgggtttttc cagtcacacc tcaggtacct ttaagaccaa 
     5101 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagagg ggactggaag 
     5161 ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag 
     5221 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct 
     5281 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag 
     5341 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag 
     5401 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc 
     5461 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg 
     5521 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca 
     5581 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg 
     5641 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg 
     5701 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc 
     5761 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat 
     5821 ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg ctagcgtttt 
     5881 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa 
     5941 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 
     6001 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 
     6061 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 
     6121 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 
     6181 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 
     6241 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 
     6301 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 
     6361 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 
     6421 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 
     6481 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 
     6541 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 
     6601 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 
     6661 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 
     6721 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc 
     6781 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 
     6841 caccgctggt agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 
     6901 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 
     6961 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 
     7021 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 
     7081 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 
     7141 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 
     7201 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 
     7261 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 
     7321 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 
     7381 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 
     7441 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 
     7501 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 
     7561 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 
     7621 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 
     7681 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 
     7741 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 
     7801 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 
     7861 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 
     7921 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 
     7981 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 
     8041 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac ttgtttattg 
     8101 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 
     8161 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga 
     8221 tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta 
     8281 ccactgccaa ttacctagtg gtttcattta ctctaaacct gtgattcctc tgaattattt 
     8341 tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt ttttaaagaa 
     8401 attgtatttg ttaaatatgt actacaaact tagtagt 
//

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