pIVT2

LOCUS pIVT2 3060 bp DNA circular SYN 15-DEC-2025 DEFINITION For making cRNA appropriate for microinjection. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3060) AUTHORS Winuthayanon W, Bernhardt ML, Padilla-Banks E, Myers PH, Edin ML, Lih FB, Hewitt SC, Korach KS, Williams CJ TITLE Oviductal estrogen receptor alpha signaling prevents protease-mediated embryo death. JOURNAL Elife. 2015 Dec 1;4:e10453. doi: 10.7554/eLife.10453. PUBMED 26623518 REFERENCE 2 (bases 1 to 3060) AUTHORS . TITLE Direct Submission JOURNAL Exported Dec 15, 2025 from SnapGene Server 8.0.1 https://www.biofeng.com COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; date: "2015-12-1"; volume: "4:e10453. doi"; pages: " 10.7554/eLife.10453" COMMENT Sequence Label: pIVT2 FEATURES Location/Qualifiers source 1..3060 /mol_type="other DNA" /organism="synthetic DNA construct" 5'UTR 8..50 /label=Xenopus globin 5'-UTR /note="translational enhancer from the 5'-UTR of the major beta-globin gene of Xenopus laevis" misc_feature 57..113 /label=MCS /note="pUC18/19 multiple cloning site" 3'UTR 151..276 /label=Xenopus globin 3'-UTR /note="3'-UTR of the major beta-globin gene of Xenopus laevis" primer_bind complement(337..354) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(337..353) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(346..368) /label=M13/pUC Forward /note="In lacZ gene" primer_bind complement(562..581) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 681..703 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(741..759) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 827..931 /gene="bla" /label=AmpR promoter CDS join(932..1000,1001..1792) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(1150..1169) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1963..2551 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2452..2471 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2705..2722 /label=L4440 /note="L4440 vector, forward primer" protein_bind 2839..2860 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter join(2875..2880,2881..2898,2899..2905) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2913..2929 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2918..2940 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 2937..2953 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 2937..2953 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" promoter 2977..2995 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 2977..2994 /label=SP6 /note="SP6 promoter, forward primer" primer_bind 3007..3026 /label=T7 /note="T7 promoter, forward primer" promoter 3007..3025 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" ORIGIN 1 aagcttgctt gttctttttg cagaagctca gaataaacgc tcaactttgg cagatcaagc 61 ttgcgtgcct gcaggtcgac tctagaggat ccccgggtac cgagctcgaa ttcgcccttc 121 tagtgactga ctaggatctg gttaccacta aaccagcctc aagaacaccc gaatggagtc 181 tctaagctac ataataccaa cttacacttt acaaaatgtt gtcccccaaa atgtagccat 241 tcgtatctgc tcctaataaa aagaaagttt cttcacattc taaaaaaaaa aaaaaaaaaa 301 aaaaaaaaaa amcccccccc ccccaagggc gaattcactg gccgtcgttt tacaacgtcg 361 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc 421 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct 481 gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca 541 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg 601 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta 661 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc 721 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat 781 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat 841 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata 901 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct 961 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa 1021 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa 1081 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt 1141 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg 1201 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca 1261 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa 1321 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt 1381 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc 1441 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa 1501 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga 1561 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc 1621 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga 1681 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga 1741 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga 1801 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat 1861 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt 1921 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 1981 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 2041 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 2101 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 2161 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 2221 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 2281 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 2341 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 2401 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 2461 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 2521 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 2581 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt 2641 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga 2701 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc 2761 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg 2821 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca 2881 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg 2941 aaacagctat gaccatgatt acgccaagct ccaacgattt aggtgacact atagaagaga 3001 aggaattaat acgactcact atagggagag agagagaatt accctcacta aagggaggag //