| 出品人: | Fodor,B.D.(2004) |
|---|---|
| 载体名称: | pMHE2 |
| 质粒类型: | 广宿主载体；细菌表达载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | T7/lac |
| 载体大小: | 5878 bp |
| 5' 测序引物及序列: | T7 promoter 5’TAATACGACTCACTATAGGG 3’ |
| 3' 测序引物及序列: | T7 terminator 5’GCTAGTTATTGCTCAGCGG 3’ |
| 载体标签: | 6XHis tag (N ter)、EK site |
| 载体抗性: | 链霉素 |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 多个菌属 |
| 备注: | -- |
| 产品目录号: | -- |
| 组成型/诱导型: | 诱导型 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS AY299693 5878 bp DNA circular SYN 10-FEB-2004
DEFINITION Broad host range expression vector pMHE2, complete sequence.
ACCESSION AY299693
VERSION AY299693.1 GI:34334140
REFERENCE 1 (bases 1 to 5878)
TITLE Modular broad-host-range expression vectors for single-protein and
protein complex purification
JOURNAL Appl. Environ. Microbiol. 70 (2), 712-721 (2004)
PUBMED 14766546
AUTHORS Fodor,B.D.
TITLE Direct Submission
JOURNAL Submitted (16-MAY-2003) Biophysics, BRC Hungarian Academy of
Sciences, Temesvari krt., Szeged, Csongrad 6701, Hungary
FEATURES Location/Qualifiers
source 1..5878
/organism="Broad host range expression vector pMHE2"
/mol_type="genomic DNA"
/db_xref="taxon:244051"
primer_bind 207..225
/note="T7 promoter primer"
regulatory 207..223
/regulatory_class="promoter"
/note="T7 promoter"
misc_feature 224..249
/note="lac operator"
regulatory 280..286
/regulatory_class="ribosome_binding_site"
misc_feature 294..296
/note="fusion protein start codon"
misc_feature 300..317
/note="His-tag"
misc_feature 318..329
/note="Factor Xa cleavage site"
primer_bind complement(428..445)
/note="T7 terminator primer"
regulatory 441..488
/regulatory_class="terminator"
/note="T7 terminator"
rep_origin 1120..1782
/note="pBBR1 oriV"
misc_feature 2776..3769
/note="RP4 mob; oriT"
regulatory complement(3859..3974)
/regulatory_class="terminator"
/note="T4 terminator"
gene complement(4234..5244)
/gene="aadA"
CDS complement(4234..5244)
/gene="aadA"
/note="streptomycin resistance"
/codon_start=1
/transl_table=11
/product="aminoglycoside-3:adenyltransferase fusion"
/protein_id="AAQ64664.1"
/db_xref="GI:34334141"
/translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQA
RYAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLL
AVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVE
VTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVAL
VGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIA
PKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVV
GK"
regulatory 5761..5876
/regulatory_class="terminator"
/note="T4 terminator"
ORIGIN
1 aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac
61 aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat
121 aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga
181 ggatcgagat ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga
241 taacaattcc cctctagaaa taattttgtt taactttaag aaggagatat accatggggc
301 atcatcatca tcatcatatc gagggaaggc ctgtcgacgg tatcgataag cttgatatcg
361 aattcctgca gcccggggga tccggctgct aacaaagccc gaaaggaagc tgagttggct
421 gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg
481 ggttttttgc tgaaaggagg aactatatcc ggatcgccct tcccaacagt tgcgcagcct
541 gaatggcgaa tggaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
601 taaatcagct cattttttaa ccaataggcc gactgcgatg agtggcaggg cggggcgtaa
661 tttttttaag gcagttattg gtgcccttaa acgcctggtg ctacgcctga ataagtgata
721 ataagcggat gaatggcaga aattcgaaag caaattcgac ccggtcgtcg gttcagggca
781 gggtcgttaa atagccgctt atgtctattg ctggtttacc ggtttattga ctaccggaag
841 cagtgtgacc gtgtgcttct caaatgcctg aggccagttt gctcaggctc tccccgtgga
901 ggtaataatt gacgatatga tcatttattc tgcctcccag agcctgataa aaacggtgaa
961 tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg
1021 cgacgcaacg cggggaggca gacaaggtat agggcggcga ggcggctaca gccgatagtc
1081 tggaacagcg cacttacggg ttgctgcgca acccaagtgc taccggcgcg gcagcgtgac
1141 ccgtgtcggc ggctccaacg gctcgccatc gtccagaaaa cacggctcat cgggcatcgg
1201 caggcgctgc tgcccgcgcc gttcccattc ctccgtttcg gtcaaggctg gcaggtctgg
1261 ttccatgccc ggaatgccgg gctggctggg cggctcctcg ccggggccgg tcggtagttg
1321 ctgctcgccc ggatacaggg tcgggatgcg gcgcaggtcg ccatgcccca acagcgattc
1381 gtcctggtcg tcgtgatcaa ccaccacggc ggcactgaac accgacaggc gcaactggtc
1441 gcggggctgg ccccacgcca cgcggtcatt gaccacgtag gccgacacgg tgccggggcc
1501 gttgagcttc acgacggaga tccagcgctc ggccaccaag tccttgactg cgtattggac
1561 cgtccgcaaa gaacgtccga tgagcttgga aagtgtcttc tggctgacca ccacggcgtt
1621 ctggtggccc atctgcgcca cgaggtgatg cagcagcatt gccgccgtgg gtttcctcgc
1681 aataagcccg gcccacgcct catgcgcttt gcgttccgtt tgcacccagt gaccgggctt
1741 gttcttggct tgaatgccga tttctctgga ctgcgtggcc atgcttatct ccatgcggta
1801 gggtgccgca cggttgcggc accatgcgca atcagctgca acttttcggc agcgcgacaa
1861 caattatgcg ttgcgtaaaa gtggcagtca attacagatt ttctttaacc tacgcaatga
1921 gctattgcgg ggggtgccgc aatgagctgt tgcgtacccc ccttttttaa gttgttgatt
1981 tttaagtctt tcgcatttcg ccctatatct agttctttgg tgcccaaaga agggcacccc
2041 tgcggggttc ccccacgcct tcggcgcggc tccccctccg gcaaaaagtg gcccctccgg
2101 ggcttgttga tcgactgcgc ggccttcggc cttgcccaag gtggcgctgc ccccttggaa
2161 cccccgcact cgccgccgtg aggctcgggg ggcaggcggg cgggcttcgc cttcgactgc
2221 ccccactcgc ataggcttgg gtcgttccag gcgcgtcaag gccaagccgc tgcgcggtcg
2281 ctgcgcgagc cttgacccgc cttccacttg gtgtccaacc ggcaagcgaa gcgcgcaggc
2341 cgcaggccgg aggcttttcc ccagagaaaa ttaaaaaaat tgatggggca aggccgcagg
2401 ccgcgcagtt ggagccggtg ggtatgtggt cgaaggctgg gtagccggtg ggcaatccct
2461 gtggtcaagc tcgtgggcag gcgcagcctg tccatcagct tgtccagcag ggttgtccac
2521 gggccgagcg aagcgagcca gccggtggcc gctcgcggcc atcgtccaca tatccacggg
2581 ctggcaaggg agcgcagcga ccgcgcaggg cgaagcccgg agagcaagcc cgtagggcgc
2641 cgcagccgcc gtaggcggtc acgactttgc gaagcaaagt ctagtgagta tactcaagca
2701 ttgagtggcc cgccggaggc accgccttgc gctgcccccg tcgagccggt tggacaccaa
2761 aagggagggg caggcatggc ggcatacgcg atcatgcgat gcaagaagct ggcgaaaatg
2821 ggcaacgtgg cggccagtct caagcacgcc taccgcgagc gcgagacgcc caacgctgac
2881 gccagcagga cgccagagaa cgagcactgg gcggccagca gcaccgatga agcgatgggc
2941 cgactgcgcg agttgctgcc agagaagcgg cgcaaggacg ctgtgttggc ggtcgagtac
3001 gtcatgacgg ccagcccgga atggtggaag tcggccagcc aagaacagca ggcggcgttc
3061 ttcgagaagg cgcacaagtg gctggcggac aagtacgggg cggatcgcat cgtgacggcc
3121 agcatccacc gtgacgaaac cagcccgcac atgaccgcgt tcgtggtgcc gctgacgcag
3181 gacggcaggc tgtcggccaa ggagttcatc ggcaacaaag cgcagatgac ccgcgaccag
3241 accacgtttg cggccgctgt ggccgatcta gggctgcaac ggggcatcga gggcagcaag
3301 gcacgtcaca cgcgcattca ggcgttctac gaggccctgg agcggccacc agtgggccac
3361 gtcaccatca gcccgcaagc ggtcgagcca cgcgcctatg caccgcaggg attggccgaa
3421 aagctgggaa tctcaaagcg cgttgagacg ccggaagccg tggccgaccg gctgacaaaa
3481 gcggttcggc aggggtatga gcctgcccta caggccgccg caggagcgcg tgagatgcgc
3541 aagaaggccg atcaagccca agagacggcc cgagaccttc gggagcgcct gaagcccgtt
3601 ctggacgccc tggggccgtt gaatcgggat atgcaggcca aggccgccgc gatcatcaag
3661 gccgtgggcg aaaagctgct gacggaacag cgggaagtcc agcgccagaa acaggcccag
3721 cgccagcagg aacgcgggcg cgcacatttc cccgaaaagt gccacctggc ggcgttgtga
3781 caatttaccg aacaactccg cggccgggaa gccgatctcg gcttgaacga attgttaggt
3841 ggcggtactt gggtcgatag ctttatgctt gtaaaccgtt ttgtgaaaaa atttttaaaa
3901 taaaaaaggg gacctctagg gtccccaatt aattagtaat ataatctatt aaaggtcatt
3961 caaaaggtca tccaccggat cagcttagta aagccctcgc tagattttaa tgcggatgtt
4021 gcgattactt cgccaactat tgcgataaca agaaaaagcc agcctttcat gatatatctc
4081 ccaatttgtg tagggcttat tatgcacgct taaaaataat aaaagcagac ttgacctgat
4141 agtttggctg tgagcaatta tgtgcttagt gcatctaacg cttgagttaa gccgcgccgc
4201 gaagcggcgt cggcttgaac gaattgttag acattatttg ccgactacct tggtgatctc
4261 gcctttcacg tagtggacaa attcttccaa ctgatctgcg cgcgaggcca agcgatcttc
4321 ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact gggccggcag
4381 gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg ttactgcgct
4441 gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc agtcgggcgg
4501 cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt caggaaccgg
4561 atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc ttgcttttgt
4621 cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg caagaatgtc
4681 attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc acggaatgat
4741 gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct ctccagggga
4801 agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat caagccttac
4861 ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat ccactgcgga
4921 gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac
4981 ctctgatagt tgagtcgata cttcggcgat caccgcttcc ctcatgatgt ttaactttgt
5041 tttagggcga ctgccctgct gcgtaacatc gttgctgctc cataacatca aacatcgacc
5101 cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtaccc caaaaaaaca
5161 gtcataacaa gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt
5221 tctggaccag ttgcgtgagc gcatacgcta cttgcattac agcttacgaa ccgaacaggc
5281 ttatgtccac tgggttcgtg ccttcatccg tttccacggt gtgcgtcacc cggcaacctt
5341 gggcagcagc gaagtcgagg catttctgtc ctggctggcg aacgagcgca aggtttcggt
5401 ctccacgcat cgtcaggcat tggcggcctt gctgttcttc tacggcaagg tgctgtgcac
5461 ggatctgccc tggcttcagg agatcggaag acctcggccg tcgcggcgct tgccggtggt
5521 gctgaccccg gatgaagtgg ttcgcatcct cggttttctg gaaggcgagc atcgtttgtt
5581 cgcccagctt ctgtatggaa cgggcatgcg gatcagtgag ggtttgcaac tgcgggtcaa
5641 ggatctggat ttcgatcacg gcacgatcat cgtgcgggag ggcaagggct ccaaggatcg
5701 ggccttgatg ttacccgaga gcttggcacc cagcctgcgc gagcagggga attgatccgg
5761 tggatgacct tttgaatgac ctttaataga ttatattact aattaattgg ggaccctaga
5821 ggtccccttt tttattttaa aaattttttc acaaaacggt ttacaagcat aaagctca
//
