| 质粒名称: | pLH-sgRNA1-boxB-MS2-PP7 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 75397 |
| 存储实验室: | Thoru Pederson |
|
载体骨架基本信息 |
|
| 载体名称: | pLKO.1 |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 8000 bp |
| 完整质粒大小: | 8337 bp |
| 载体类型: | 哺乳动物表达，慢病毒，CRISPR |
| 筛选标记: | 潮霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | sgRNA1-boxB-MS2-PP7 |
| 物种来源: | Synthetic |
| 插入大小: | 146 bp |
| 启动子 | U6 |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | Sbf I (未破坏) |
| 3' 克隆位点: | EcoR I (未破坏) |
| 5' 测序引物 | cccttcaccgagggcctatttccc |
| 3' 测序引物 | CTATTCTTTCCCCTGCACTGTACCC |
|
文献引用方法 |
|
| 材料和方法部分: | pLH-sgRNA1-boxB-MS2-PP7 was a gift from Thoru Pederson (Addgene plasmid # 75397 ; http://n2t.net/addgene:75397 ; RRID:Addgene_75397) |
| 参考文献部分: | Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow. Ma H, Tu LC, Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T. Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526. 10.1038/nbt.3526 PubMed 27088723 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLH-sgRNA1-boxB-MS2-PP7 8337 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION sgRNA1-boxB-MS2-PP7.
ACCESSION .
VERSION .
KEYWORDS pLH-sgRNA1-boxB-MS2-PP7
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8337)
AUTHORS Ma H, Tu LC, Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T
TITLE Multiplexed labeling of genomic loci with dCas9 and engineered
sgRNAs using CRISPRainbow.
JOURNAL Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
PUBMED 27088723
REFERENCE 2 (bases 1 to 8337)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8337
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 94..111
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 228..249
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 264..294
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 302..318
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 307..329
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 326..342
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 326..342
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 361..381
/label=T3
/note="T3 promoter, forward primer"
promoter 363..381
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 409..635
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 636..816
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 863..988
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1481..1714
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
promoter 2241..2481
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
primer_bind 2241..2261
/label=hU6-F
/note="Human U6 promoter, forward primer"
primer_bind 2412..2431
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
CDS 2830..3135
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind 3014..3033
/label=ccdB-fwd
/note="ccdB gene, forward primer"
misc_RNA 3228..3246
/label=MS2 stem loop
/note="stem loop that binds the bacteriophage MS2 coat
protein"
misc_feature 3350..3467
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 3516..4026
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
primer_bind 3945..3963
/label=hPGK-F
/note="Human PGK promoter, forward primer"
CDS 4116..5141
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
E"
primer_bind complement(5165..5181)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5166..5182)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 5264..5497
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 5569..5690
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5606..5625)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5660..5679
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 5730..5865
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 5792..5811
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(5885..5904)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(5886..5904)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(5914..5931)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(5914..5930)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5923..5945)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 6072..6527
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6159..6178)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6369..6390
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 6553..6657
/gene="bla"
/label=AmpR promoter
CDS 6658..7518
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6876..6895)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7689..8277
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8178..8197
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
61 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
121 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
181 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
241 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
301 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
361 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg
421 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
481 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
541 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
601 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
661 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
721 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
781 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
841 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
901 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
961 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
1021 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
1081 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
1141 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
1201 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
1261 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
1321 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
1381 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
1441 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
1501 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
1561 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
1621 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
1681 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
1741 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
1801 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
1861 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
1921 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
1981 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
2041 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
2101 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
2161 gagacagatc cattcgatta gtgaacggat ctcgacggta tcgatcacga gactagcctc
2221 gagcggccgc ccccttcacc gagggcctat ttcccatgat tccttcatat ttgcatatac
2281 gatacaaggc tgttagagag ataattggaa ttaatttgac tgtaaacaca aagatattag
2341 tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat
2401 gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt
2461 tatatatctt gtggaaagga cgaaacaccg gtgtcttcga ggcttactaa aagccagata
2521 acagtatgcg tatttgcgcg ctgatttttg cggtataaga atatatactg atatgtatac
2581 ccgaagtatg tcaaaaagag gtgtgctatg aagcagcgta ttacagtgac agttgacagc
2641 gacagctatc agttgctcaa ggcatatatg atgtcaatat ctccggtctg gtaagcacaa
2701 ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag
2761 ggatggctga ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac gagaacaggg
2821 actggtgaaa tgcagtttaa ggtttacacc tataaaagag agagccgtta tcgtctgttt
2881 gtggatgtac agagtgatat tattgacacg cccgggcgac ggatggtgat ccccctggcc
2941 agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt acccggtggt gcatatcggg
3001 gatgaaagct ggcgcatgat gaccaccgat atggccagtg tgccggtctc cgttatcggg
3061 gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg
3121 ttctggggaa tataaaccct gcaggacgaa gacccgtttg agagctaggg ccctgaagaa
3181 gggccctagc aagttcaaat aaggctagtc cgttatcaac ttggccaaca tgaggatcac
3241 ccatgtctgc agggccaagt ggcaccgagt cggtgcggag cagacgatat ggcgtcgctc
3301 ctttttttga attctcgacc tcgagacaaa tggcagtatt catccacaat tttaaaagaa
3361 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
3421 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
3481 gggacagcag agatccactt tggccgcggc tcgagggggt tggggttgcg ccttttccaa
3541 ggcagccctg ggtttgcgca gggacgcggc tgctctgggc gtggttccgg gaaacgcagc
3601 ggcgccgacc ctgggtctcg cacattcttc acgtccgttc gcagcgtcac ccggatcttc
3661 gccgctaccc ttgtgggccc cccggcgacg cttcctgctc cgcccctaag tcgggaaggt
3721 tccttgcggt tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct cactagtacc
3781 ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc
3841 aatagcggct gctcagcagg gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag
3901 gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc
3961 aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg accgaatcac cgacctctct
4021 ccccaggggg atccaccgga tcaagcttgc cagctggggc gccctctggt aaggttggga
4081 agccctgcag atctgatccc ggggggcaat gagatatgaa aaagcctgaa ctcaccgcga
4141 cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct
4201 cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc
4261 gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat
4321 cggccgcgct cccgattccg gaagtgcttg acattgggga atttagcgag agcctgacct
4381 attgcatctc ccgccgtgca cagggtgtca cgttgcaaga tctgcctgaa accgaactgc
4441 ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc
4501 agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg
4561 atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca
4621 ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc
4681 ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg
4741 gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg
4801 tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact
4861 tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca
4921 ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg
4981 cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa
5041 tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg
5101 gaaaccgacg ccccagcact cgtccgaggg caaaggaata gagtagatgc cgaccgggat
5161 ctatcgatac cgtcgacctc gagggggggc ccggtacctt taagaccaat gacttacaag
5221 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
5281 tcccaacgaa gaaaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
5341 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
5401 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
5461 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
5521 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
5581 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
5641 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
5701 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
5761 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
5821 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa
5881 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga
5941 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
6001 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
6061 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
6121 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
6181 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
6241 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
6301 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
6361 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
6421 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
6481 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
6541 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
6601 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
6661 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
6721 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
6781 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
6841 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
6901 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
6961 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
7021 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
7081 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
7141 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
7201 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
7261 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
7321 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
7381 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
7441 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
7501 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
7561 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
7621 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
7681 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
7741 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
7801 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
7861 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
7921 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
7981 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
8041 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
8101 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
8161 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
8221 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
8281 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatg
//
