pCMV-PKA

价格:2000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clonetech
载体名称: pCMV-PKA
质粒类型: 哺乳动物表达载体;基因载体;信号通路报告载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: CMV
载体大小: 5.0 kb
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签:
载体抗性: 卡那霉素
筛选标记: Neomycin
克隆菌株: HB101、DH5alpha;JM109(单链DNA复制)
宿主细胞(系): 常规细胞系,如293、Hela等
备注: pCMV-PKA载体是小鼠蛋白激酶A(PKA)的表达载体;
CMV启动子驱动PKA基因的过表达;
用于研究细胞PKA信号通路,结合激酶定量分析试剂盒可以定量分析信号通路中的蛋白的功能。
产品目录号: 631927
稳定性: 瞬表达 或 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pCMV-PKA载体图谱
pCMV-PKA 载体特征

载体简介

pCMV-PKA载体介绍

pCMV-PKA is designed for the constitutive expression of Protein Kinase A (PKA) (mouse) in mammalian cells. It can also be used to study the effects of a given stimulus on the PKA signaling pathway. In conjunction with Clontech’s In Vivo Kinase Assay Kits, pCMV-PKA is an ideal positive control for studying a gene or molecule and its affect on kinase activation in the PKA pathway. cAMP, a ubiquitous second messenger, regulates expression of cellular functions including metabolism, cell proliferation, and neuronal signaling via activation of the PKA-dependent phosphorylation of the CREB transcription factor (1–3). pCMV-PKA contains the gene encoding the catalytic subunit of PKA, which is driven by the human cytomegalovirus (CMV) promoter.
SV40 polyadenylation signals downstream of the PKA gene direct proper processing of the 3’ end of the mRNA. The vector backbone also contains an SV40 origin for replication in mammalian cells expressing the SV40 T-antigen. A neomycin resistance cassette (Neor)—consisting of the SV40 early promoter, the neomycin/kanamycin resistance gene of Tn5, and polyadenylation signals from the Herpes simplex virus thymidine kinase (HSV TK) gene—allows stably transfected eukaryotic cells to be selected using G418 (4). A bacterial promoter upstream of this cassette expresses kanamycin resistance in E. coli. The pCMV-PKA backbone also provides a pUC origin of replication for propagation in E. coli and an f1 origin for single-stranded DNA production. The recombinant vector can be transfected into mammalian cells using any standard method.

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