pSP64

价格:750元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Promega
载体名称: pSP64
质粒类型: 酵母双杂交系统载体
高拷贝/低拷贝: 高拷贝
启动子: --
克隆方法: 多克隆位点,限制性内切酶
载体大小: 2999bp
5' 测序引物及序列: Sp6 Fwd: ATTTAGGTGACACTATAG
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素(Ampicillian)
筛选标记: --
备注: --
产品目录号: --
稳定性: 瞬表达
组成型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pSP64载体图谱



pSP64多克隆位点

pSP64载体特征

载体简介

The pSP64 Poly(A) Vector can be used as a standard cloning vector and for in vitro transcription 
from the SP6 promoter. The pSP64 Poly(A) Vector also can be used to generate poly(A)+ transcripts 
in vitro. The vector has a stretch of 30 dA:dT residues inserted between the SacI and EcoRI sites. 
Therefore, when foreign DNA is cloned into any polylinker site other than EcoRI (HindIII, PstI, SalI, 
AccI, HincII, XbaI, BamHI, AvaI, SmaI or SacI), linearization of the recombinant plasmid with EcoRI 
allows the use of SP6 RNA polymerase in vitro to prepare RNA copies of the inserted sequences that 
contain a synthetic 3′ "poly(A)" tail of 30 residues.

载体序列

LOCUS       pSP64	2999 bp 	DNA	SYN
DEFINITION  pSP64
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="pSP64"
                     /mol_type="other DNA"
     promoter        complement(63..81)
                     /label="M13_reverse_primer"
     misc_feature    complement(80..102)
                     /label="M13_pUC_rev_primer"
     promoter        complement(116..145)
                     /label="lac_promoter"
     gene            complement(1228..2088)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(1228..2088)
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(2130..2158)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    complement(2317..2339)
                     /label="pGEX_3_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     gene            complement(2490..2741)
                     /label="tet (887 - 636)"
                     /gene="tet (887 - 636)"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    2673..2692
                     /label="pBRrevBam_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    2983..1
                     /label="Sp6_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 gaatacaagc ttgggctgca ggtcgactct agaggatccc cgggcgagct cgaattcgta
   61 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
  121 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
  181 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
  241 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
  301 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
  361 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
  421 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
  481 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
  541 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
  601 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
  661 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
  721 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
  781 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
  841 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
  901 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
  961 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
 1021 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
 1081 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
 1141 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
 1201 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
 1261 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
 1321 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
 1381 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
 1441 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
 1501 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
 1561 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
 1621 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
 1681 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
 1741 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
 1801 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
 1861 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
 1921 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
 1981 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
 2041 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
 2101 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
 2161 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
 2221 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
 2281 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
 2341 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
 2401 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
 2461 gagagtgcac catatcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt
 2521 agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg
 2581 cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg
 2641 agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca
 2701 accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatctggcta
 2761 gcgatgaccc tgctgattgg ttcgctgacc atttccgggg tgcggaacgg cgttaccaga
 2821 aactcagaag gttcgtccaa ccaaaccgac tctgacggca gtttacgaga gagatgatag
 2881 ggtctgcttc agtaagccag atgctacaca attaggcttg tacatattgt cgttagaacg
 2941 cggctacaat taatacataa ccttatgtat catacacata cgatttaggt gacactata

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