pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ARHGEF10-FLAG

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ARHGEF10-FLAG                6569 bp DNA     circular SYN 18-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6569)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6569
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..3282
                     /codon_start=1
                     /label=ARHGEF10
                     /translation="VYKGLF*RKKMKSELRTAAEFSYSFPGSRTNLGDRRSLQLCSIRS
                     LLPSRSPGSTAYRWPSSP*KRRTTWAGSVWKTMGITLKRRSILSSSDTCP*WWPSSRSS
                     RLNVLLITLNLT*IMKASQIHFPRHMVSCGSEVAPIKWVRLPSSRFKIPLPKSLSASTW
                     NLASCACCTFPSRRSAESLGHPRTPRPRP*ELLMSPRSV*GRRREAFPFIKAVKAPRK*
                     DFSTFSLLRSPQS*AWLARLRACTLAWSTGQSPATPEPQMDPGIQNLKK*SS*ASYQLE
                     VYS*WKTRCGRLPEVKSSSSVWRLML*RVSWRPTRRKAW*SPTWPCPASGSGLPSPQGP
                     RSAFFTRKLSSTCRTSTSPPLFTTCCQGTSGCR*RACSSATDC*WSAPAWESSWPCRSH
                     VCKGFPK*PEEAWSPTMHTTVLSNSSSWPRLCTRKTRTNPGTAWLLAPSLRTKTRRTHF
                     RVEELVHL*ARVTLTQPSGWEIRWDR*LRKATCPPHLGP*ACLTAPAL*STDQRTAPSM
                     IS*RILSR*EAKHAGPRKPRPARRWWSVEGRATAGCTGRPGSPTRKSWRRPSWSGRSLC
                     *I"
     misc_feature    2162..2167
                     /label=SDS
     misc_feature    2492..2497
                     /label=SDS
     misc_feature    3020..3025
                     /label=SDS
     CDS             3289..3354
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3358..3789
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3502..3507
                     /label=SDS
     repeat_region   3832..3972
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    3832..3961
                     /label=AAV2 ITR
     rep_origin      4047..4502
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4784..4888
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4889..5749
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5920..6508
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggttt acaaaggctt
     1561 attttgaaga aagaagatga aatcagagct gcggactgct gcagaattca gttacagctt
     1621 cccgggaagc aggacaaatc tgggcgaccg acgttcttta cagctgtgtt caatacgttc
     1681 actcctgcca tcaaggagtc ctgggtcaac agcttacaga tggccaagct cgccctagaa
     1741 gaggagaacc acatgggctg gttctgtgtg gaagacgatg ggaatcacat taaaaaggag
     1801 aagcatcctc tcctcgtcgg acacatgccc gtgatggtgg ccaagcagca ggagttcaag
     1861 attgaatgtg ctgcttataa ccctgaacct tacctaaata atgaaagcca gccagattca
     1921 ttttccacgg cacatggttt cctgtggatc ggaagttgca cccatcaaat gggtcagatt
     1981 gccatcgtct cgtttcaaaa ttccactccc aaagtcattg agtgcttcaa cgtggaatct
     2041 cgcatcctgt gcatgctgta cgttcccgtc gaggagaagc gcagagagcc tggggcaccc
     2101 ccggaccccg agaccccggc cgtgagagct tctgatgtcc ccacgatctg tgtagggacg
     2161 gaggagggaa gcatttccat ttataaaagc agtcaaggct ccaagaaagt gagacttcag
     2221 cactttttca ctcctgagaa gtccacagtc atgagcctgg cttgcacgtc tcagagcctg
     2281 tacgctggcc tggtcaacgg ggcagtcgcc agctacgcca gagccccaga tggatcctgg
     2341 gattcagaac ctcaaaaagt gatcaagtta ggcgtcctac cagttagaag tctactcatg
     2401 atggaagaca cgttgtgggc ggcttccgga ggtcaagtct tcatcatcag tgtggagact
     2461 catgctgtag agggtcagct ggaggcccac caggaggaag gcatggtgat ctcccacatg
     2521 gccgtgtccg gcgtcgggat ctggattgcc ttcacctcag ggtccacgct ccgccttttt
     2581 cacacggaaa ctctcaagca cctgcaggac atcaacatcg ccacccctgt tcacaacatg
     2641 ctgccagggc accagcggct gtcggtgacg agcctgctcg tctgccacgg attgctgatg
     2701 gtcggcacca gcctgggagt cctcgtggcc ctgccggtcc cacgtctgca agggattccc
     2761 aaagtgaccg gaagaggcat ggtctcctac catgcacaca acagtcctgt caaattcatc
     2821 gtcctggcca cggctctgca cgagaaagac aaggacaaat ccagggacag cctggctcct
     2881 ggccccgagc ctcaggacga agaccagaag gacgcacttc cgagtggagg agctggttca
     2941 tctctgagcc agggtgaccc tgacgcagcc atctggttgg gagattcgct gggatcgatg
     3001 actcagaaaa gcgacctgtc ctcctcatct gggtccctga gcttgtctca cggctccagc
     3061 tctctagagc acagatcaga ggacagcacc atctatgatc tcctgaagga tcctgtctcg
     3121 ctgagaagca aagcacgccg ggccaagaaa gccaaggcca gctcggcgct ggtggtctgt
     3181 ggagggcagg gccaccgccg ggtgcacagg aaggcccggc agccccacca ggaagagctg
     3241 gcgccgaccg tcatggtctg gcagatccct ctgctgaata tatgtacaga ctacaaagac
     3301 catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga caagtaactc
     3361 gagataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     3421 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     3481 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttagttctt gccacggcgg
     3541 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     3601 attccgtggt gtttatttgt gaaatttgtg atgctattgc tttatttgta accatctagc
     3661 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     3721 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag
     3781 gttttttaaa gatctggaga ccggtgtggc acgtgcggac cgagcggccg caggaacccc
     3841 tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac
     3901 caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca
     3961 gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     4021 accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg
     4081 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccttagcgc ccgctccttt
     4141 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     4201 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     4261 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     4321 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaactc
     4381 tatctcgggc tattcttttg atttataagg gattttgccg atttcggtct attggttaaa
     4441 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
     4501 tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     4561 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     4621 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     4681 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     4741 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     4801 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     4861 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     4921 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     4981 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     5041 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     5101 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     5161 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     5221 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     5281 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     5341 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     5401 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     5461 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     5521 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     5581 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     5641 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     5701 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     5761 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     5821 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     5881 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     5941 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     6001 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     6061 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     6121 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     6181 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     6241 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     6301 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     6361 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     6421 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     6481 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     6541 ggccttttgc tggccttttg ctcacatgt
//