| 质粒名称: | pLenti-CMV-MCS-GFP-SV-puro |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 73582 |
| 存储实验室: | Paul Odgren |
|
载体骨架基本信息 |
|
| 载体名称: | pLenti-CMV-MCs-SV-puro |
| 载体出品公司: | gift from Dr. Hong Zhang, University of Massachusetts Medical School |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 8829 bp |
| 载体修饰: | GFP insert |
| 载体类型: | 慢病毒表达 |
| 筛选标记: | 嘌呤霉素 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 标签/融合蛋白: | GFP tag（主干上的C终端） |
| 克隆方法: | 限制性内切酶 |
| 5' 测序引物 | GAGCACCCAGTCCGCCCTG |
|
文献引用方法 |
|
| 材料和方法部分: | pLenti-CMV-MCS-GFP-SV-puro was a gift from Paul Odgren (Addgene plasmid # 73582 ; http://n2t.net/addgene:73582 ; RRID:Addgene_73582) |
| 参考文献部分: | Studies of OC-STAMP in Osteoclast Fusion: A New Knockout Mouse Model, Rescue of Cell Fusion, and Transmembrane Topology. Witwicka H, Hwang SY, Reyes-Gutierrez P, Jia H, Odgren PE, Donahue LR, Birnbaum MJ, Odgren PR. PLoS One. 2015 Jun 4;10(6):e0128275. doi: 10.1371/journal.pone.0128275. eCollection 2015. PONE-D-14-50827 [pii] PubMed 26042409 |
买家导航
pLenti-CMV-MCS-GFP-SV-puro载体质粒基本信息
pLenti-CMV-MCS-GFP-SV-puro质粒图谱载体图谱和pLenti-CMV-MCS-GFP-SV-puro载体序列质粒序列多克隆位点信息
pLenti-CMV-MCS-GFP-SV-puro质粒载体简介
pLenti-CMV-MCS-GFP-SV-puro质粒序列载体序列
LOCUS pLenti-CMV-MCS-GFP-SV-puro 8455 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Third generation lentiviral vector expressing GFP and puromycin.
ACCESSION .
VERSION .
KEYWORDS pLenti-CMV-MCS-GFP-SV-puro
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8455)
AUTHORS Witwicka H, Hwang SY, Reyes-Gutierrez P, Jia H, Odgren PE, Donahue
LR, Birnbaum MJ, Odgren PR
TITLE Studies of OC-STAMP in Osteoclast Fusion: A New Knockout Mouse
Model, Rescue of Cell Fusion, and Transmembrane Topology.
JOURNAL PLoS One. 2015 Jun 4;10(6):e0128275. doi:
10.1371/journal.pone.0128275. eCollection 2015.
PUBMED 26042409
REFERENCE 2 (bases 1 to 8455)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8455
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 113..134
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 149..179
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 187..203
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 192..214
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 211..227
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 211..227
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 246..266
/label=T3
/note="T3 promoter, forward primer"
promoter 248..266
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 294..520
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 521..701
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 748..873
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1366..1599
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2095..2211
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
enhancer 2234..2537
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2538..2741
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2692..2712
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 2738..2762
/label=LNCX
/note="Human CMV promoter, forward primer"
CDS 2818..3537
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(2863..2884)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(3124..3143)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 3471..3492
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(3582..3602)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 3587..3916
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3767..3902
/label=SV40 ori
/note="SV40 origin of replication"
CDS 3926..4525
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(3926..3945)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 4422..4442
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 4592..5180
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4645..4665)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5063..5074)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 5267..5500
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 5572..5693
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5609..5628)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5663..5682
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 5733..5868
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(5888..5907)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(5889..5907)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(5917..5934)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(5917..5933)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5926..5948)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 6075..6530
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6162..6181)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6372..6393
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 6556..6660
/gene="bla"
/label=AmpR promoter
CDS 6661..7521
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6879..6898)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7692..8280
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8181..8200
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 8434..8451
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
61 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
121 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
181 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
241 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc
301 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
361 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
421 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg
481 cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca
541 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
601 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
661 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
721 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
781 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
841 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
901 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
961 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
1021 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
1081 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
1141 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
1201 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
1261 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
1321 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
1381 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
1441 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
1501 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
1561 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
1621 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
1681 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
1741 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
1801 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
1861 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
1921 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
1981 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
2041 agacagagac agatccattc gattagtgaa cggatctcga cggtatcggt taacttttaa
2101 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
2161 agacatacaa actaaagaat tacaaaaaca aattacaaaa ttcaaaattt tatcgataag
2221 cttgggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
2281 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
2341 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
2401 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
2461 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
2521 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
2581 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
2641 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
2701 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
2761 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccga ctctagaatg
2821 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
2881 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
2941 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
3001 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
3061 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
3121 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
3181 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
3241 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
3301 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
3361 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
3421 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
3481 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaacgg
3541 gatccggact agttaacgcg tcgaccagtg gtcgaccctg tggaatgtgt gtcagttagg
3601 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
3661 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
3721 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
3781 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
3841 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
3901 cctaggcttt tgcaaaaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac
3961 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
4021 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
4081 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
4141 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
4201 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
4261 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
4321 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
4381 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
4441 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
4501 catgacccgc aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag
4561 gagcgcacga ccccatgcat cgccagtcga caatcaacct ctggattaca aaatttgtga
4621 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
4681 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
4741 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
4801 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
4861 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
4921 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
4981 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
5041 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
5101 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
5161 cctttgggcc gcctccccgc ctggaattcg agctcggtac ctttaagacc aatgacttac
5221 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
5281 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
5341 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
5401 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
5461 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
5521 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
5581 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
5641 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
5701 tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
5761 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
5821 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc
5881 caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg
5941 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
6001 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
6061 gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
6121 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
6181 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
6241 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
6301 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
6361 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
6421 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
6481 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact
6541 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
6601 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
6661 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
6721 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
6781 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
6841 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
6901 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
6961 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
7021 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
7081 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
7141 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
7201 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
7261 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
7321 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
7381 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
7441 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
7501 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
7561 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
7621 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
7681 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
7741 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
7801 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
7861 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
7921 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
7981 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
8041 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
8101 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
8161 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
8221 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
8281 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
8341 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
8401 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaag
//
