PX396

价格:1000元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Addgene
载体名称: PX396 或 PX396 Parvibaculum lavamentivorans Cas9
质粒类型: CRISPR-Cas9载体;基因敲除载体;昆虫细胞载体
高拷贝/低拷贝: 高拷贝
插入位点: 限制性内切酶,多克隆位点
启动子: CMV
载体大小: 10307 bp
5' 测序引物及序列: CMV Forward:CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列: C-CMV-24 (5'-TATTAGGACAAGGCTGGTGGGCAC-3')
载体标签: NLS (C ter);HA、NLS (N ter)
载体抗性: 氨苄青霉素
筛选标记: Neomycin
克隆菌株: Stbl3
宿主细胞(系): 昆虫细胞
备注: --
产品目录号: #68333
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 杆状病毒

载体质粒图谱和多克隆位点信息

PX396载体图谱



载体简介

载体序列

LOCUS       PX396 Parvibaculum lavamentivorans Cas9	10307 bp 	DNA	SYN
DEFINITION  PX396 Parvibaculum lavamentivorans Cas9
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..10307
                     /organism="PX396 Parvibaculum lavamentivorans Cas9"
                     /mol_type="other DNA"
     misc_feature    47..1001
                     /label="Ac_5_flank"
     CDS             complement(343..948)
                     /label="ORF frame 3"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     promoter        1023..1587
                     /label="CMV_immearly_promoter"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1090..1377
                     /label="CAG_enhancer"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1544..1564
                     /label="CMV_fwd_primer"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     promoter        1615..1633
                     /label="T7_promoter"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1633..1656
                     /label="lacO"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1772..1796
                     /label="attB1"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     CDS             complement(1795..2445)
                     /label="ORF frame 3"
                     /translation="MGVDSSLAVRRGSAGQPCSQCGLFIFQGLQSGCSLVSGFLLIVH
                     IRVGCIGFLQFSAFKMPVLGQMIDCPSELVSTQAFLQSPSPQFIRLVGHDHGPICRPV
                     SWQKAGFVQCLIQCLSANPSPSQLAPHHAFLSPLLVQGGPIWVPCLRENAHAQPLDVA
                     CGLGATVINHAESDTCCADVKAENPLHGRFDLALFLRRSVIWNIVWVHGGTGGISP*"
     CDS             1822..4998
                     /label="ORF frame 1"
                     /translation="MYPYDVPDYASPKKKRKVEASMERIFGFDIGTTSIGFSVIDYSS
                     TQSAGNIQRLGVRIFPEARDPDGTPLNQQRRQKRMMRRQLRRRRIRRKALNETLHEAG
                     FLPAYGSADWPVVMADEPYELRRRGLEEGLSAYEFGRAIYHLAQHRHFKGRELEESDT
                     PDPDVDDEKEAANERAATLKALKNEQTTLGAWLARRPPSDRKRGIHAHRNVVAEEFER
                     LWEVQSKFHPALKSEEMRARISDTIFAQRPVFWRKNTLGECRFMPGEPLCPKGSWLSQ
                     QRRMLEKLNNLAIAGGNARPLDAEERDAILSKLQQQASMSWPGVRSALKALYKQRGEP
                     GAEKSLKFNLELGGESKLLGNALEAKLADMFGPDWPAHPRKQEIRHAVHERLWAADYG
                     ETPDKKRVIILSEKDRKAHREAAANSFVADFGITGEQAAQLQALKLPTGWEPYSIPAL
                     NLFLAELEKGERFGALVNGPDWEGWRRTNFPHRNQPTGEILDKLPSPASKEERERISQ
                     LRNPTVVRTQNELRKVVNNLIGLYGKPDRIRIEVGRDVGKSKREREEIQSGIRRNEKQ
                     RKKATEDLIKNGIANPSRDDVEKWILWKEGQERCPYTGDQIGFNALFREGRYEVEHIW
                     PRSRSFDNSPRNKTLCRKDVNIEKGNRMPFEAFGHDEDRWSAIQIRLQGMVSAKGGTG
                     MSPGKVKRFLAKTMPEDFAARQLNDTRYAAKQILAQLKRLWPDMGPEAPVKVEAVTGQ
                     VTAQLRKLWTLNNILADDGEKTRADHRHHAIDALTVACTHPGMTNKLSRYWQLRDDPR
                     AEKPALTPPWDTIRADAEKAVSEIVVSHRVRKKVSGPLHKETTYGDTGTDIKTKSGTY
                     RQFVTRKKIESLSKGELDEIRDPRIKEIVAAHVAGRGGDPKKAFPPYPCVSPGGPEIR
                     KVRLTSKQQLNLMAQTGNGYADLGSNHHIAIYRLPDGKADFEIVSLFDASRRLAQRNP
                     IVQRTRADGASFVMSLAAGEAIMIPEGSKKGIWIVQGVWASGQVVLERDTDADHSTTT
                     RPMPNPILKDDAKKVSIDPIGRVRPSND*"
     misc_feature    1825..1851
                     /label="HA"
                     /translation="MYPYDVPDYASPKKKRKVEASMERIFGFDIGTTSIGFSVIDYSS
                     TQSAGNIQRLGVRIFPEARDPDGTPLNQQRRQKRMMRRQLRRRRIRRKALNETLHEAG
                     FLPAYGSADWPVVMADEPYELRRRGLEEGLSAYEFGRAIYHLAQHRHFKGRELEESDT
                     PDPDVDDEKEAANERAATLKALKNEQTTLGAWLARRPPSDRKRGIHAHRNVVAEEFER
                     LWEVQSKFHPALKSEEMRARISDTIFAQRPVFWRKNTLGECRFMPGEPLCPKGSWLSQ
                     QRRMLEKLNNLAIAGGNARPLDAEERDAILSKLQQQASMSWPGVRSALKALYKQRGEP
                     GAEKSLKFNLELGGESKLLGNALEAKLADMFGPDWPAHPRKQEIRHAVHERLWAADYG
                     ETPDKKRVIILSEKDRKAHREAAANSFVADFGITGEQAAQLQALKLPTGWEPYSIPAL
                     NLFLAELEKGERFGALVNGPDWEGWRRTNFPHRNQPTGEILDKLPSPASKEERERISQ
                     LRNPTVVRTQNELRKVVNNLIGLYGKPDRIRIEVGRDVGKSKREREEIQSGIRRNEKQ
                     RKKATEDLIKNGIANPSRDDVEKWILWKEGQERCPYTGDQIGFNALFREGRYEVEHIW
                     PRSRSFDNSPRNKTLCRKDVNIEKGNRMPFEAFGHDEDRWSAIQIRLQGMVSAKGGTG
                     MSPGKVKRFLAKTMPEDFAARQLNDTRYAAKQILAQLKRLWPDMGPEAPVKVEAVTGQ
                     VTAQLRKLWTLNNILADDGEKTRADHRHHAIDALTVACTHPGMTNKLSRYWQLRDDPR
                     AEKPALTPPWDTIRADAEKAVSEIVVSHRVRKKVSGPLHKETTYGDTGTDIKTKSGTY
                     RQFVTRKKIESLSKGELDEIRDPRIKEIVAAHVAGRGGDPKKAFPPYPCVSPGGPEIR
                     KVRLTSKQQLNLMAQTGNGYADLGSNHHIAIYRLPDGKADFEIVSLFDASRRLAQRNP
                     IVQRTRADGASFVMSLAAGEAIMIPEGSKKGIWIVQGVWASGQVVLERDTDADHSTTT
                     RPMPNPILKDDAKKVSIDPIGRVRPSND*"
     misc_feature    1855..1875
                     /label="SV40 NLS"
                     /translation="MYPYDVPDYASPKKKRKVEASMERIFGFDIGTTSIGFSVIDYSS
                     TQSAGNIQRLGVRIFPEARDPDGTPLNQQRRQKRMMRRQLRRRRIRRKALNETLHEAG
                     FLPAYGSADWPVVMADEPYELRRRGLEEGLSAYEFGRAIYHLAQHRHFKGRELEESDT
                     PDPDVDDEKEAANERAATLKALKNEQTTLGAWLARRPPSDRKRGIHAHRNVVAEEFER
                     LWEVQSKFHPALKSEEMRARISDTIFAQRPVFWRKNTLGECRFMPGEPLCPKGSWLSQ
                     QRRMLEKLNNLAIAGGNARPLDAEERDAILSKLQQQASMSWPGVRSALKALYKQRGEP
                     GAEKSLKFNLELGGESKLLGNALEAKLADMFGPDWPAHPRKQEIRHAVHERLWAADYG
                     ETPDKKRVIILSEKDRKAHREAAANSFVADFGITGEQAAQLQALKLPTGWEPYSIPAL
                     NLFLAELEKGERFGALVNGPDWEGWRRTNFPHRNQPTGEILDKLPSPASKEERERISQ
                     LRNPTVVRTQNELRKVVNNLIGLYGKPDRIRIEVGRDVGKSKREREEIQSGIRRNEKQ
                     RKKATEDLIKNGIANPSRDDVEKWILWKEGQERCPYTGDQIGFNALFREGRYEVEHIW
                     PRSRSFDNSPRNKTLCRKDVNIEKGNRMPFEAFGHDEDRWSAIQIRLQGMVSAKGGTG
                     MSPGKVKRFLAKTMPEDFAARQLNDTRYAAKQILAQLKRLWPDMGPEAPVKVEAVTGQ
                     VTAQLRKLWTLNNILADDGEKTRADHRHHAIDALTVACTHPGMTNKLSRYWQLRDDPR
                     AEKPALTPPWDTIRADAEKAVSEIVVSHRVRKKVSGPLHKETTYGDTGTDIKTKSGTY
                     RQFVTRKKIESLSKGELDEIRDPRIKEIVAAHVAGRGGDPKKAFPPYPCVSPGGPEIR
                     KVRLTSKQQLNLMAQTGNGYADLGSNHHIAIYRLPDGKADFEIVSLFDASRRLAQRNP
                     IVQRTRADGASFVMSLAAGEAIMIPEGSKKGIWIVQGVWASGQVVLERDTDADHSTTT
                     RPMPNPILKDDAKKVSIDPIGRVRPSND*"
     misc_feature    1861..1875
                     /label="NLS"
                     /translation="MYPYDVPDYASPKKKRKVEASMERIFGFDIGTTSIGFSVIDYSS
                     TQSAGNIQRLGVRIFPEARDPDGTPLNQQRRQKRMMRRQLRRRRIRRKALNETLHEAG
                     FLPAYGSADWPVVMADEPYELRRRGLEEGLSAYEFGRAIYHLAQHRHFKGRELEESDT
                     PDPDVDDEKEAANERAATLKALKNEQTTLGAWLARRPPSDRKRGIHAHRNVVAEEFER
                     LWEVQSKFHPALKSEEMRARISDTIFAQRPVFWRKNTLGECRFMPGEPLCPKGSWLSQ
                     QRRMLEKLNNLAIAGGNARPLDAEERDAILSKLQQQASMSWPGVRSALKALYKQRGEP
                     GAEKSLKFNLELGGESKLLGNALEAKLADMFGPDWPAHPRKQEIRHAVHERLWAADYG
                     ETPDKKRVIILSEKDRKAHREAAANSFVADFGITGEQAAQLQALKLPTGWEPYSIPAL
                     NLFLAELEKGERFGALVNGPDWEGWRRTNFPHRNQPTGEILDKLPSPASKEERERISQ
                     LRNPTVVRTQNELRKVVNNLIGLYGKPDRIRIEVGRDVGKSKREREEIQSGIRRNEKQ
                     RKKATEDLIKNGIANPSRDDVEKWILWKEGQERCPYTGDQIGFNALFREGRYEVEHIW
                     PRSRSFDNSPRNKTLCRKDVNIEKGNRMPFEAFGHDEDRWSAIQIRLQGMVSAKGGTG
                     MSPGKVKRFLAKTMPEDFAARQLNDTRYAAKQILAQLKRLWPDMGPEAPVKVEAVTGQ
                     VTAQLRKLWTLNNILADDGEKTRADHRHHAIDALTVACTHPGMTNKLSRYWQLRDDPR
                     AEKPALTPPWDTIRADAEKAVSEIVVSHRVRKKVSGPLHKETTYGDTGTDIKTKSGTY
                     RQFVTRKKIESLSKGELDEIRDPRIKEIVAAHVAGRGGDPKKAFPPYPCVSPGGPEIR
                     KVRLTSKQQLNLMAQTGNGYADLGSNHHIAIYRLPDGKADFEIVSLFDASRRLAQRNP
                     IVQRTRADGASFVMSLAAGEAIMIPEGSKKGIWIVQGVWASGQVVLERDTDADHSTTT
                     RPMPNPILKDDAKKVSIDPIGRVRPSND*"
     CDS             complement(3273..3998)
                     /label="ORF frame 1"
                     /translation="MSGHSLFSWARICFAAYLVSFSCRAAKSSGMVLARKRLTFPGLI
                     PVPPLADTMPCSLIWMALHRSSSWPNASKGILFPFSMFTSLRHSVLFLGLLSKLRERG
                     QMCSTSYLPSLNRALKPIWSPVYGHLSWPSFHRIHFSTSSRLGLAIPFLIRSSVAFFL
                     CFSFRRMPDWISSLSLLDFPTSRPTSIRIRSGFPYSPIRLLTTFLSSFCVRTTVGLRS
                     WLMRSLSSFEAGLGSLSRISPVG*"
     CDS             complement(3316..3798)
                     /label="ORF frame 3"
                     /translation="MAKCLERHPISLLDVHVFATQCLIPWAVIKTSRARPDVFHLISA
                     FSKQGIEANLVAGVWASFLALFPQDPLLHIVPARISNSVFDQIFSGFLSLLLVPSNAR
                     LDFLPFSFGLPDVPAHFNPDSIGFSIQPDQIVDHLSQLILCSDHGWVAQLANAFPFLF
                     *"
     CDS             complement(3847..4332)
                     /label="ORF frame 3"
                     /translation="MQWAADFLSHPVTDHNFRHGFLSVGADGIPGWSQSWLLCSWIVA
                     QLPIPAQLVGHSRMSAGHCQSVDGMVPVICSGFLPIVSQNIVQSPQFAQLGGDLSSHC
                     FDLHWSLRSHVWPQPFQLGQDLLCSVSCVVQLPGCKILRHGLSQEAFDLSGAHPSSPF
                     G*"
     misc_feature    complement(5031..5048)
                     /label="Sp6_primer"
                     /translation="MQWAADFLSHPVTDHNFRHGFLSVGADGIPGWSQSWLLCSWIVA
                     QLPIPAQLVGHSRMSAGHCQSVDGMVPVICSGFLPIVSQNIVQSPQFAQLGGDLSSHC
                     FDLHWSLRSHVWPQPFQLGQDLLCSVSCVVQLPGCKILRHGLSQEAFDLSGAHPSSPF
                     G*"
     misc_feature    5053..5077
                     /label="attB2"
                     /translation="MQWAADFLSHPVTDHNFRHGFLSVGADGIPGWSQSWLLCSWIVA
                     QLPIPAQLVGHSRMSAGHCQSVDGMVPVICSGFLPIVSQNIVQSPQFAQLGGDLSSHC
                     FDLHWSLRSHVWPQPFQLGQDLLCSVSCVVQLPGCKILRHGLSQEAFDLSGAHPSSPF
                     G*"
     terminator      5089..5555
                     /label="hGH_PA_terminator"
                     /translation="MQWAADFLSHPVTDHNFRHGFLSVGADGIPGWSQSWLLCSWIVA
                     QLPIPAQLVGHSRMSAGHCQSVDGMVPVICSGFLPIVSQNIVQSPQFAQLGGDLSSHC
                     FDLHWSLRSHVWPQPFQLGQDLLCSVSCVVQLPGCKILRHGLSQEAFDLSGAHPSSPF
                     G*"
     terminator      5561..5596
                     /label="T7_terminator"
                     /translation="MQWAADFLSHPVTDHNFRHGFLSVGADGIPGWSQSWLLCSWIVA
                     QLPIPAQLVGHSRMSAGHCQSVDGMVPVICSGFLPIVSQNIVQSPQFAQLGGDLSSHC
                     FDLHWSLRSHVWPQPFQLGQDLLCSVSCVVQLPGCKILRHGLSQEAFDLSGAHPSSPF
                     G*"
     rep_origin      5713..6019
                     /label="f1_origin"
                     /translation="MQWAADFLSHPVTDHNFRHGFLSVGADGIPGWSQSWLLCSWIVA
                     QLPIPAQLVGHSRMSAGHCQSVDGMVPVICSGFLPIVSQNIVQSPQFAQLGGDLSSHC
                     FDLHWSLRSHVWPQPFQLGQDLLCSVSCVVQLPGCKILRHGLSQEAFDLSGAHPSSPF
                     G*"
     misc_feature    complement(6133..6153)
                     /label="pBABE_3_primer"
                     /translation="MQWAADFLSHPVTDHNFRHGFLSVGADGIPGWSQSWLLCSWIVA
                     QLPIPAQLVGHSRMSAGHCQSVDGMVPVICSGFLPIVSQNIVQSPQFAQLGGDLSSHC
                     FDLHWSLRSHVWPQPFQLGQDLLCSVSCVVQLPGCKILRHGLSQEAFDLSGAHPSSPF
                     G*"
     misc_feature    complement(6139..6354)
                     /label="SV40_enhancer"
                     /translation="MQWAADFLSHPVTDHNFRHGFLSVGADGIPGWSQSWLLCSWIVA
                     QLPIPAQLVGHSRMSAGHCQSVDGMVPVICSGFLPIVSQNIVQSPQFAQLGGDLSSHC
                     FDLHWSLRSHVWPQPFQLGQDLLCSVSCVVQLPGCKILRHGLSQEAFDLSGAHPSSPF
                     G*"
     promoter        6151..6419
                     /label="SV40_promoter"
                     /translation="MQWAADFLSHPVTDHNFRHGFLSVGADGIPGWSQSWLLCSWIVA
                     QLPIPAQLVGHSRMSAGHCQSVDGMVPVICSGFLPIVSQNIVQSPQFAQLGGDLSSHC
                     FDLHWSLRSHVWPQPFQLGQDLLCSVSCVVQLPGCKILRHGLSQEAFDLSGAHPSSPF
                     G*"
     rep_origin      6318..6395
                     /label="SV40_origin"
                     /translation="MQWAADFLSHPVTDHNFRHGFLSVGADGIPGWSQSWLLCSWIVA
                     QLPIPAQLVGHSRMSAGHCQSVDGMVPVICSGFLPIVSQNIVQSPQFAQLGGDLSSHC
                     FDLHWSLRSHVWPQPFQLGQDLLCSVSCVVQLPGCKILRHGLSQEAFDLSGAHPSSPF
                     G*"
     misc_feature    6380..6399
                     /label="SV40pro_F_primer"
                     /translation="MQWAADFLSHPVTDHNFRHGFLSVGADGIPGWSQSWLLCSWIVA
                     QLPIPAQLVGHSRMSAGHCQSVDGMVPVICSGFLPIVSQNIVQSPQFAQLGGDLSSHC
                     FDLHWSLRSHVWPQPFQLGQDLLCSVSCVVQLPGCKILRHGLSQEAFDLSGAHPSSPF
                     G*"
     CDS             6534..7328
                     /label="ORF frame 3"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     gene            6537..7325
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     CDS             complement(6843..7379)
                     /label="ORF frame 1"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     terminator      7505..7624
                     /label="SV40_PA_terminator"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    7593..7612
                     /label="EBV_rev_primer"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     terminator      7815..7850
                     /label="T7_terminator"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    complement(7873..7890)
                     /label="Baculovirs_rev_primer"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    7880..7957
                     /label="3_AcPH"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     CDS             complement(7987..8604)
                     /label="ORF frame 3"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     misc_feature    8584..8617
                     /label="loxP"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     gene            complement(9385..10245)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     CDS             complement(9385..10245)
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
   61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
  121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
  181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
  241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
  301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
  361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
  421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
  481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
  541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
  601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
  661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
  721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
  781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
  841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
  901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
  961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc
 1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
 1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
 1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
 1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
 1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
 1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
 1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
 1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
 1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
 1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac
 1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac
 1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac
 1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg
 1801 agatatacca ccggtgccac catgtaccca tacgatgttc cagattacgc ttcgccgaag
 1861 aaaaagcgca aggtcgaagc gtccatggag aggattttcg gctttgacat cggcacaaca
 1921 agtatcggat tcagcgtgat tgattacagt agcacccagt ccgcaggcaa catccagagg
 1981 ctgggcgtgc gcattttccc tgaggcaagg gacccagatg ggacccccct gaaccagcag
 2041 cggagacaga aacgcatgat gaggcgccag ctgcgacgga gaaggattcg ccgaaaggca
 2101 ctgaatgaga cactgcacga agccggcttt ctgccagctt acgggtctgc agattggccc
 2161 gtggtcatgg ccgacgagcc ttatgaactg cggagaaggg gactggagga aggcctgagt
 2221 gcttacgagt tcggacgggc aatctatcat ctggcccagc accggcattt taaaggcaga
 2281 gaactggagg aatccgatac acccgaccct gatgtggacg atgagaagga agccgctaac
 2341 gagagagcag ccactctgaa ggccctgaaa aatgaacaga ccacactggg agcatggctg
 2401 gcccgccgac ccccttctga ccgcaagcga ggaatccacg cccataggaa cgtggtcgct
 2461 gaggagttcg agcgcctgtg ggaagtgcag tccaagtttc accccgctct gaaatctgag
 2521 gaaatgcggg caagaatcag tgatacaatt ttcgcccaga ggcctgtgtt ttggcgcaag
 2581 aacactctgg gagagtgcag attcatgcct ggcgaaccac tgtgtcccaa ggggtcctgg
 2641 ctgtctcagc agcggagaat gctggagaaa ctgaacaatc tggctatcgc aggcgggaat
 2701 gctaggccac tggatgcaga ggaacgcgac gccattctga gtaagctgca gcagcaggcc
 2761 agcatgtcct ggccaggcgt gcggtcagct ctgaaggcac tgtacaaaca gagaggcgag
 2821 cccggggctg aaaagagcct gaaattcaac ctggagctgg gaggcgaatc caagctgctg
 2881 ggaaatgccc tggaggctaa actggcagat atgtttggcc ctgactggcc agctcacccc
 2941 cgaaagcagg agatccggca cgcagtgcat gaacggctgt gggctgcaga ttacggcgag
 3001 acacccgaca agaaaagagt catcattctg tccgagaagg atcgaaaagc tcatcgggaa
 3061 gccgctgcaa actctttcgt ggcagacttt ggaattactg gcgagcaggc agctcagctg
 3121 caggccctga agctgccaac cggctgggaa ccttatagca tcccagcact gaacctgttc
 3181 ctggccgagc tggaaaaggg ggagaggttt ggagccctgg tgaatggacc tgattgggaa
 3241 ggctggaggc gcacaaactt cccccaccgc aatcagccta ctggggagat cctggacaag
 3301 ctgccaagtc ccgcctcaaa agaggaaagg gaacgcatta gccagctgcg caacccaacc
 3361 gtggtccgaa cacagaatga gctgagaaag gtggtcaaca atctgatcgg gctgtatgga
 3421 aaacccgatc gaatccggat tgaagtgggc cgggacgtcg ggaagtccaa aagagaaagg
 3481 gaggaaatcc agtctggcat tcgacggaac gagaagcaga gaaagaaagc cactgaagat
 3541 ctgatcaaaa acggaattgc taatcctagc cgggacgatg tggagaagtg gatcctgtgg
 3601 aaagagggcc aggaaagatg cccatacacc ggcgaccaga ttggcttcaa tgccctgttt
 3661 agagaaggca gatatgaggt ggaacacatc tggcctcgct ctcgaagttt tgataacagc
 3721 ccaaggaata agacactgtg tcgcaaagac gtgaacatcg agaagggaaa taggatgcct
 3781 ttcgaggcat ttggccatga cgaagatcgg tggagcgcca tccagattag actgcagggc
 3841 atggtgtcag ccaaaggggg aactgggatg agccccggaa aggtcaaacg cttcctggct
 3901 aagaccatgc ctgaggattt tgcagcccgg cagctgaacg acacaagata cgctgcaaag
 3961 cagatcctgg cccagctgaa aaggctgtgg ccagacatgg gacctgaggc tccagtgaag
 4021 gtcgaagcag tgactggaca ggtcaccgcc cagctgcgca aactgtggac tctgaacaat
 4081 attctggctg acgatgggga gaaaaccaga gcagatcaca ggcaccatgc catcgacgct
 4141 ctgacagtgg cctgcactca tcctggaatg accaacaagc tgagcaggta ttggcagctg
 4201 cgcgacgatc cacgagcaga gaagccagct ctgactccac cctgggatac catccgcgcc
 4261 gacgctgaga aagccgtgtc tgaaattgtg gtcagtcacc gggtgagaaa gaaagtcagc
 4321 ggcccactgc ataaggagac tacctacggc gatacaggga ctgacattaa gaccaaatcc
 4381 ggcacatata gacagttcgt gaccaggaag aaaatcgagt cactgagcaa gggggagctg
 4441 gatgaaattc gcgacccccg aatcaaagaa attgtggcag ctcacgtcgc aggacgagga
 4501 ggcgacccca agaaggcctt ccctccatac ccctgtgtgt ctcccggagg ccctgagatc
 4561 cggaaggtca gactgaccag taaacagcag ctgaacctga tggcccagac agggaatgga
 4621 tacgctgacc tgggctccaa ccaccatatc gcaatctacc ggctgcccga tgggaaggcc
 4681 gacttcgaga ttgtgtcact gtttgatgct agcagaaggc tggcacagag aaatccaatc
 4741 gtgcagagga cacgagcaga cggagccagc ttcgtcatgt ccctggcagc cggagaggcc
 4801 atcatgattc ccgaaggctc aaagaaaggg atctggattg tgcagggagt ctgggcaagc
 4861 ggacaggtgg tcctggagag ggacaccgat gctgaccact ctacaactac ccgccctatg
 4921 ccaaacccca tcctgaagga cgatgccaag aaagtgagta tcgatcctat tggccgagtc
 4981 cggccatcaa atgactaaga attcggtacc gagctcaagc ttgcggccgc ctatagtgtc
 5041 acctaaatcg taacccagct ttcttgtaca aagtggtcgg gtggcatccc tgtgacccct
 5101 ccccagtgcc tctcctggcc ctggaagttg ccactccagt gcccaccagc cttgtcctaa
 5161 taaaattaag ttgcatcatt ttgtctgact aggtgtcctt ctataatatt atggggtgga
 5221 ggggggtggt atggagcaag gggcaagttg ggatgacaac ctgtagggcc tgcggggtct
 5281 attgggaacc aagctggagt gcagtggcac aatcttggct cactgcaatc tccgcctcct
 5341 gggttcaagc gattctcctg cctcagcctc ccgagttgtt gggattccag gcatgcatga
 5401 ccaggctcag ctaatttttg tttttttggt agagacgggg tttcaccata ttggccaggc
 5461 tggtctccaa ctcctaatct caggtgatct acccaccttg gcctcccaaa ttgctgggat
 5521 tacaggcgtg aaccactgct cccttccctg tccttctagc ataacccctt ggggcctcta
 5581 aacgggtctt gaggggtttt ttgtctagaa gacaatagca ggcatgctgg ggatgcggtg
 5641 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
 5701 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
 5761 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
 5821 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
 5881 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
 5941 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
 6001 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
 6061 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
 6121 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
 6181 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
 6241 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
 6301 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
 6361 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
 6421 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
 6481 cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg
 6541 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
 6601 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
 6661 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
 6721 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
 6781 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
 6841 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
 6901 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
 6961 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
 7021 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
 7081 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
 7141 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
 7201 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
 7261 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
 7321 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
 7381 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
 7441 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
 7501 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
 7561 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
 7621 ttatcatgtc tgttcatgaa gccccttgag catctgactt ctggctaata aaggaaattt
 7681 attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg
 7741 agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc
 7801 catatgtaac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg
 7861 aaagcatgcg gaggaaattc tccttgaagt ttccctggtg ttcaaagtaa aggagtttgc
 7921 accagacgca cctctgttca ctggtccggc gtattaaaac acgatacatt gttattagta
 7981 catttattaa gcgctagatt ctgtgcgttg ttgatttaca gacaattgtt gtacgtattt
 8041 taataattca ttaaatttat aatctttagg gtggtatgtt agagcgaaaa tcaaatgatt
 8101 ttcagcgtct ttatatctga atttaaatat taaatcctca atagatttgt aaaataggtt
 8161 tcgattagtt tcaaacaagg gttgtttttc cgaaccgatg gctggactat ctaatggatt
 8221 ttcgctcaac gccacaaaac ttgccaaatc ttgtagcagc aatctagctt tgtcgatatt
 8281 cgtttgtgtt ttgttttgta ataaaggttc gacgtcgttc aaaatattat gcgcttttgt
 8341 atttctttca tcactgtcgt tagtgtacaa ttgactcgac gtaaacacgt taaatagagc
 8401 ttggacatat ttaacatcgg gcgtgttagc tttattaggc cgattatcgt cgtcgtccca
 8461 accctcgtcg ttagaagttg cttccgaaga cgattttgcc atagccacac gacgcctatt
 8521 aattgtgtcg gctaacacgt ccgcgatcaa atttgtagtt gagctttttg gaattgcgat
 8581 cgcataactt cgtatagcat acattatacg aagttataag ctcggaacgc tgcgctcggt
 8641 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
 8701 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
 8761 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc cccctgacga gcatcacaaa
 8821 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
 8881 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
 8941 tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc
 9001 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
 9061 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
 9121 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
 9181 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
 9241 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
 9301 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
 9361 aaaggatctc aagaagatcc tttgttacca atgcttaatc agtgaggcac ctatctcagc
 9421 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
 9481 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
 9541 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
 9601 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
 9661 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
 9721 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
 9781 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
 9841 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
 9901 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
 9961 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
10021 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
10081 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
10141 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
10201 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
10261 atattattga agcatttatc agggttattg tctcatgtcc gcgcgtt
//

载体质粒图谱pdf版和相关资料下载

载体应用举例

分享到:
Copyright © 20012-2013 BIOFENG. 生物风 版权所有 Powered by Biofeng