| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-WNK3-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001558 |
| 产品用途: | 3' AAVLINK plasmid for WNK3 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | WNK3 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv1-pCALM1-Cre-3'-WNK3-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv1-pCALM1-Cre-3'-WNK3-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-WNK3-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-WNK3-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-WNK3-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-WNK3-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-WNK3-FLAG 6489 bp DNA circular SYN 12-JAN-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6489)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 18, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6489
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3202
/codon_start=1
/label=WNK3
/translation="GIQRPILLLCQAVEPFICRQEVDILA*ALLVLVSKILLARNPGLA
N*KAGHTGYGSQPAFSRDQKSVKWKQKR*DQQLLLIPFL*HGSPQLILGL*IDVKR*VD
HFSGVGSR*LQFLSSSQQK*HLLE*NTYQCSVRQTILVKKPLLKQQSLSW*K*NLPHKI
QKLRFLMRSYKLFRKPVKKIKEFPNKVTTSYLSAQLVRLMYLQ*PQKRNLKKLQPQEVA
CSLDLNCCLKRERY*LLGNSLALIVNFQPVLLAVESQWQRLVQRVISAYHTTKNKLMLK
HRVHSSIRHLPQ*AVMMNQK*RMRT*RWSFKDYEKNTFRRW*IFKPSRIRSCRSSMNAF
GQLKIAKPNLLRFLCHLHHHVDQDLSKANFEAAPSP*HMWTMA*LLQGKAAL*MSWKIH
CVWRVMQHHANSLQPVKKGCSQMTYTSWWMTGQRKQ*EILLLSQV*TNLNKVNTN*RQK
TGIKYLKILRLLWATHQHGFLLCPKSVELSQLPCHKDSHSLHFLGHYHHMECLTFVSIM
LWRGRGIQYSG*EFQEQHNNL**FPPNLGDHSSQG*ICRHFQLHQCRILPQSLLVL"
misc_feature 1540..1545
/label=SDS
misc_feature 2439..2444
/label=SDS
misc_feature 3188..3193
/label=SDS
CDS 3209..3274
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3278..3709
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3422..3427
/label=SDS
repeat_region 3752..3892
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3752..3881
/label=AAV2 ITR
rep_origin 3967..4422
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4704..4808
/gene="bla"
/label=AmpR promoter
CDS 4809..5669
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5840..6428
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggggatccag aggcctatcc
1501 tgctgctgtg tcaagcggtg gagccattca tctgcagaca ggaggtggat attttggcct
1561 aagctttact tgtcctagtc tcaaaaatcc tattagcaag aaatcctgga ctcgcaaatt
1621 aaaaagctgg gcatacaggc tacggcagtc aaccagcttt ttcaagagat caaaagtccg
1681 tcaagtggaa acagaagaga tgagatcagc aattgctcct gatcccattc ctctgacacg
1741 ggagtccaca gctgatacta gggctttgaa tagatgtaaa gcgatgagtg gatcatttca
1801 gcggggtcgg ttccaggtga ttacaattcc tcagcagcag tcagcaaaaa tgacatcttt
1861 tggaatagaa cacatatcag tgttcagtga gacaaaccat tctagtgaag aagcctttat
1921 taaaacagca aagtctcagt tggtagaaat agaacctgcc acacaaaatc caaaaacttc
1981 gttttcttat gagaagttac aagctcttca ggaaacctgt aaagaaaata aaggagttcc
2041 caaacaaggt gacaacttct tatctttcag cgcagcttgt gagactgatg tatcttcagt
2101 gaccccagaa aaggaatttg aagaaacttc agccacagga agtagcatgc agtctggatc
2161 tgaactgttg cttaaagaga gagagatatt gactgctggg aaacagccta gctctgatag
2221 tgaattttca gccagtcttg ctggcagtgg aaagtcagtg gcaaagactg gtccagagag
2281 taatcagtgc ttaccacacc acgaagaaca agcttatgct caaacacaga gttcactctt
2341 ctattcgcca tcttccccaa tgagcagtga tgatgaatca gaaatagagg atgaggactt
2401 gaaggtggag cttcaaagat tacgagaaaa acacattcag gaggtggtaa atcttcaaac
2461 ccagcagaat aaggagctgc aggagctcta tgaacgcctt cggtcaatta aagatagcaa
2521 aacccaatct actgagattc ctttgccacc tgcatcacca cgtcgaccaa gatctttcaa
2581 aagcaaactt cgaagccgcc cccagtcctt gacacatgtg gacaatggca tagttgctac
2641 agggaaaagc tgccttataa atgagttgga aaatccactg tgtgtggaga gtaatgcagc
2701 atcatgccaa cagtctccag ccagtaaaaa agggatgttc acagatgact tacacaagct
2761 ggtggatgac tggacaaagg aagcagtagg aaattctctt attaagccaa gtttaaacca
2821 acttaaacaa agtcaacaca aactagagac agaaaactgg aataaagtat ctgaaaatac
2881 tccgtctact atgggctaca catcaacatg gatttcttct ctgtcccaaa tccgtggagc
2941 tgtcccaact tccttgccac aaggactctc actcccttca tttcctgggc cattatcatc
3001 atatggaatg cctcacgttt gtcagtataa tgctgtggcg ggggcggggt atccagtaca
3061 gtgggtagga atttcaggaa caacacaaca atctgtagta attcccgccc aatctggggg
3121 accattccag ccagggatga atatgcaggc atttccaact tcatcagtgc agaatcctgc
3181 cacaatccct cctggtccta aatgtacaga ctacaaagac catgacggtg attataaaga
3241 tcatgacatc gactacaagg atgacgatga caagtaactc gagataatca acctctggat
3301 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
3361 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
3421 tcctccttgt ataaatcctg gttagttctt gccacggcgg aactcatcgc cgcctgcctt
3481 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gtttatttgt
3541 gaaatttgtg atgctattgc tttatttgta accatctagc tttatttgtg aaatttgtga
3601 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
3661 cattcatttt atgtttcagg ttcaggggga gatgtgggag gttttttaaa gatctggaga
3721 ccggtgtggc acgtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact
3781 ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg
3841 ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg
3901 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac
3961 catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
4021 tgaccgctac acttgccagc gccttagcgc ccgctccttt cgctttcttc ccttcctttc
4081 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
4141 gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta
4201 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
4261 atagtggact cttgttccaa actggaacaa cactcaactc tatctcgggc tattcttttg
4321 atttataagg gattttgccg atttcggtct attggttaaa aaatgagctg atttaacaaa
4381 aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta
4441 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
4501 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
4561 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
4621 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
4681 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
4741 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
4801 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
4861 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
4921 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
4981 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
5041 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
5101 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
5161 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
5221 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
5281 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
5341 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
5401 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
5461 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
5521 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
5581 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
5641 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
5701 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
5761 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
5821 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
5881 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
5941 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
6001 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
6061 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
6121 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
6181 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
6241 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
6301 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
6361 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
6421 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
6481 ctcacatgt
//
