| 质粒名称: | Desmoplakin I no force control (F40-based) |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 119187 |
| 存储实验室: | Alex Dunn |
|
载体骨架基本信息 |
|
| 载体名称: | pSBtet-Pur |
| 载体出品公司: | Eric Kowarz |
| 空载体大小: | 5569 bp |
| 完整质粒大小: | 13466 bp |
| 载体类型: | 哺乳细胞表达、Transposon |
| 筛选标记: | Puromycin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | human Desmoplakinhuman Desmoplakin I (1-1945)-[mTFP1-F40-mEYFP] / Desmoplakin I no force control (F40-based) / DPI-ctrl |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 7897 bp |
| 基因突变: | truncation after aa1945 |
| GenBank编号 | NM_004415.3 |
| 启动子: | TCE |
| 标签/融合蛋白: | mTFP1-F40-mEYFP (C terminal on insert) |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | SfiI (not destroyed) |
| 3’克隆位点: | eSfiI (not destroyed) |
| 5’测序引物 | CCTGGAGCCAATTCCAACTCT |
| 3’测序引物 | CACTGCATTCTTGTTGTGGTT |
|
文献引用方法 |
|
| 材料和方法部分: | Desmoplakin I no force control (F40-based) was a gift from Alex Dunn (Addgene plasmid # 119187 ; http://n2t.net/addgene:119187 ; RRID:Addgene_119187) |
| 参考文献部分: | Mechanical loading of desmosomes depends on the magnitude and orientation of external stress. Price AJ, Cost AL, Ungewiss H, Waschke J, Dunn AR, Grashoff C. Nat Commun. 2018 Dec 11;9(1):5284. doi: 10.1038/s41467-018-07523-0. 10.1038/s41467-018-07523-0 PubMed 30538252 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS Desmoplakin I no force control (F40-based) 13466 bp ds-DNA circular SYN 13-MAY-2021
DEFINITION The no force control for the F40-based human desmoplakin I tension
sensor serves to detect changes in FRET between mTFP1 and mEYFP that
are tension-independent..
ACCESSION .
VERSION .
KEYWORDS Desmoplakin I no force control (F40-based)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13466)
AUTHORS Price AJ, Cost AL, Ungewiss H, Waschke J, Dunn AR, Grashoff C
TITLE Mechanical loading of desmosomes depends on the magnitude and
orientation of external stress.
JOURNAL Nat Commun. 2018 Dec 11;9(1):5284. doi: 10.1038/s41467-018-07523-0.
PUBMED 30538252
REFERENCE 2 (bases 1 to 13466)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..13466
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 295..607
/label=tight TRE promoter
/note="Tet-responsive promoter PTight, consisting of seven
tet operator sequences followed by the minimal CMV
promoter"
protein_bind 303..321
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 339..357
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 374..392
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 410..428
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 446..464
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 481..499
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 517..535
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
primer_bind 580..604
/label=LNCX
/note="Human CMV promoter, forward primer"
CDS 6928..7632
/codon_start=1
/product="monomeric teal (cyan) fluorescent protein (Ai et
al., 2006)"
/label=mTFP1
/note="mammalian codon-optimized"
/translation="VSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDGT
NTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTFE
DKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLKG
DVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESAV
ARNSTDGMDELYK"
CDS 7753..8469
/codon_start=1
/product="enhanced YFP with monomerizing A206K mutation
(Zacharias et al., 2002)"
/label=mEYFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(7798..7819)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(8059..8078)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 8406..8427
/label=EGFP-C
/note="EGFP, forward primer"
polyA_signal 8602..8723
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(8613..8632)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 8667..8686
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
CDS 9446..10189
/codon_start=1
/product="improved tetracycline-controlled transactivator"
/label=rtTA-Advanced
/note="In the Tet-On(R) system, rtTA-Advanced binds to the
Tet-responsive element and stimulates transcription only in
the presence of tetracycline or doxycycline."
/translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
DALDDFDLDMLPADALDDFDLDMLPG"
CDS 10196..10252
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 10253..10852
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(10253..10272)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 10749..10769
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
primer_bind complement(10907..10924)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 10913..11137
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(11463..11480)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(11634..12222)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(11714..11733)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(12393..13253)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 13016..13035
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(13254..13358)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ggatccctat acagttgaag tcggaagttt acatacactt aagttggagt cattaaaact
61 cgtttttcaa ctactccaca aatttcttgt taacaaacaa tagttttggc aagtcagtta
121 ggacatctac tttgtgcatg acacaagtca tttttccaac aattgtttac agacagatta
181 tttcacttat aattcactgt atcacaattc cagtgggtca gaagtttaca tacactaagt
241 tcgactcctc tgcagaatgc ggcgatgttt cggtaagggg tccgctatct agacgagttt
301 actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg
361 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt
421 gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta
481 tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta
541 tgtcgattag gcgtgtacgg tgggcgccta taaaagcaga gctcgtttag tgaaccgtca
601 gatcgcctgg agccaattcc aactcttttg ccttatacct actttccgta ccacttccta
661 ccctcgaaag gcctctgcgg ccgcgtcaac agcccctgct ccttgggccc ctccatgcca
721 tgccgtaatc tctcccaccc gaccaacacc aacacccagc tccgacgcag ctcctctgcg
781 cccttgccgc cctccgagcc acagctttcc tcccgctcct gcccccggcc cgtcgccgtc
841 tccgcgctcg cagcggcctc gggagggccc aggtagcgag cagcgacctc gcgagccttc
901 cgcactcccg cccggttccc cggccgtccg cctatccttg gccccctccg ctttctccgc
961 gccggcccgc ctcgcttatg cctcggcgct gagccgctct cccgattgcc cgccgacatg
1021 agctgcaacg gaggctccca cccgcggatc aacactctgg gccgcatgat ccgcgccgag
1081 tctggcccgg acctgcgcta cgaggtgacc agcggcggcg ggggcaccag caggatgtac
1141 tattctcggc gcggcgtgat caccgaccag aactcggacg gctactgtca aaccggcacg
1201 atgtccaggc accagaacca gaacaccatc caggagctgc tgcagaactg ctccgactgc
1261 ttgatgcgag cagagctcat cgtgcagcct gaattgaagt atggagatgg aatacaactg
1321 actcggagtc gagaattgga tgagtgtttt gcccaggcca atgaccaaat ggaaatcctc
1381 gacagcttga tcagagagat gcggcagatg ggccagccct gtgatgctta ccagaaaagg
1441 cttcttcagc tccaagagca aatgcgagcc ctttataaag ccatcagtgt ccctcgagtc
1501 cgcagggcca gctccaaggg tggtggaggc tacacttgtc agagtggctc tggctgggat
1561 gagttcacca aacatgtcac cagtgaatgt ttggggtgga tgaggcagca aagggcggag
1621 atggacatgg tggcctgggg tgtggacctg gcctcagtgg agcagcacat taacagccac
1681 cggggcatcc acaactccat cggcgactat cgctggcagc tggacaaaat caaagccgac
1741 ctgcgcgaga aatctgcgat ctaccagttg gaggaggagt atgaaaacct gctgaaagcg
1801 tcctttgaga ggatggatca cctgcgacag ctgcagaaca tcattcaggc cacgtccagg
1861 gagatcatgt ggatcaatga ctgcgaggag gaggagctgc tgtacgactg gagcgacaag
1921 aacaccaaca tcgctcagaa acaggaggcc ttctccatac gcatgagtca actggaagtt
1981 aaagaaaaag agctcaataa gctgaaacaa gaaagtgacc aacttgtcct caatcagcat
2041 ccagcttcag acaaaattga ggcctatatg gacactctgc agacgcagtg gagttggatt
2101 cttcagatca ccaagtgcat tgatgttcat ctgaaagaaa atgctgccta ctttcagttt
2161 tttgaagagg cgcagtctac tgaagcatac ctgaaggggc tccaggactc catcaggaag
2221 aagtacccct gcgacaagaa catgcccctg cagcacctgc tggaacagat caaggagctg
2281 gagaaagaac gagagaaaat ccttgaatac aagcgtcagg tgcagaactt ggtaaacaag
2341 tctaagaaga ttgtacagct gaagcctcgt aacccagact acagaagcaa taaacccatt
2401 attctcagag ctctctgtga ctacaaacaa gatcagaaaa tcgtgcataa gggggatgag
2461 tgtatcctga aggacaacaa cgagcgcagc aagtggtacg tgacgggccc gggaggcgtt
2521 gacatgcttg ttccctctgt ggggctgatc atccctcctc cgaacccact ggccgtggac
2581 ctctcttgca agattgagca gtactacgaa gccatcttgg ctctgtggaa ccagctctac
2641 atcaacatga agagcctggt gtcctggcac tactgcatga ttgacataga gaagatcagg
2701 gccatgacaa tcgccaagct gaaaacaatg cggcaggaag attacatgaa gacgatagcc
2761 gaccttgagt tacattacca agagttcatc agaaatagcc aaggctcaga gatgtttgga
2821 gatgatgaca agcggaaaat acagtctcag ttcaccgatg cccagaagca ttaccagacc
2881 ctggtcattc agctccctgg ctatccccag caccagacag tgaccacaac tgaaatcact
2941 catcatggaa cctgccaaga tgtcaaccat aataaagtaa ttgaaaccaa cagagaaaat
3001 gacaagcaag aaacatggat gctgatggag ctgcagaaga ttcgcaggca gatagagcac
3061 tgcgagggca ggatgactct caaaaacctc cctctagcag accaggggtc ttctcaccac
3121 atcacagtga aaattaacga gcttaagagt gtgcagaatg attcacaagc aattgctgag
3181 gttctcaacc agcttaaaga tatgcttgcc aacttcagag gttctgaaaa gtactgctat
3241 ttacagaatg aagtatttgg actatttcag aaactggaaa atatcaatgg tgttacagat
3301 ggctacttaa atagcttatg cacagtaagg gcactgctcc aggctattct ccaaacagaa
3361 gacatgttaa aggtttatga agccaggctc actgaggagg aaactgtctg cctggacctg
3421 gataaagtgg aagcttaccg ctgtggactg aagaaaataa aaaatgactt gaacttgaag
3481 aagtcgttgt tggccactat gaagacagaa ctacagaaag cccagcagat ccactctcag
3541 acttcacagc agtatccact ttatgatctg gacttgggca agttcggtga aaaagtcaca
3601 cagctgacag accgctggca aaggatagat aaacagatcg actttagatt atgggacctg
3661 gagaaacaaa tcaagcaatt gaggaattat cgtgataact atcaggcttt ctgcaagtgg
3721 ctctatgatg ctaaacgccg ccaggattcc ttagaatcca tgaaatttgg agattccaac
3781 acagtcatgc ggtttttgaa tgagcagaag aacttgcaca gtgaaatatc tggcaaacga
3841 gacaaatcag aggaagtaca aaaaattgct gaactttgcg ccaattcaat taaggattat
3901 gagctccagc tggcctcata cacctcagga ctggaaactc tgctgaacat acctatcaag
3961 aggaccatga ttcagtcccc ttctggggtg attctgcaag aggctgcaga tgttcatgct
4021 cggtacattg aactacttac aagatctgga gactattaca ggttcttaag tgagatgctg
4081 aagagtttgg aagatctgaa gctgaaaaat accaagatcg aagttttgga agaggagctc
4141 agactggccc gagatgccaa ctcggaaaac tgtaataaga acaaattcct ggatcagaac
4201 ctgcagaaat accaggcaga gtgttcccag ttcaaagcga agcttgcgag cctggaggag
4261 ctgaagagac aggctgagct ggatgggaag tcggctaagc aaaatctaga caagtgctac
4321 ggccaaataa aagaactcaa tgagaagatc acccgactga cttatgagat tgaagatgaa
4381 aagagaagaa gaaaatctgt ggaagacaga tttgaccaac agaagaatga ctatgaccaa
4441 ctgcagaaag caaggcaatg tgaaaaggag aaccttggtt ggcagaaatt agagtctgag
4501 aaagccatca aggagaagga gtacgagatt gaaaggttga gggttctact gcaggaagaa
4561 ggcacccgga agagagaata tgaaaatgag ctggcaaagg taagaaacca ctataatgag
4621 gagatgagta atttaaggaa caagtatgaa acagagatta acattacgaa gaccaccatc
4681 aaggagatat ccatgcaaaa agaggatgat tccaaaaatc ttagaaacca gcttgataga
4741 ctttcaaggg aaaatcgaga tctgaaggat gaaattgtca ggctcaatga cagcatcttg
4801 caggccactg agcagcgaag gcgagctgaa gaaaacgccc ttcagcaaaa ggcctgtggc
4861 tctgagataa tgcagaagaa gcagcatctg gagatagaac tgaagcaggt catgcagcag
4921 cgctctgagg acaatgcccg gcacaagcag tccctggagg aggctgccaa gaccattcag
4981 gacaaaaata aggagatcga gagactcaaa gctgagtttc aggaggaggc caagcgccgc
5041 tgggaatatg aaaatgaact gagtaaggta agaaacaatt atgatgagga gatcattagc
5101 ttaaaaaatc agtttgagac cgagatcaac atcaccaaga ccaccatcca ccagctcacc
5161 atgcagaagg aagaggatac cagtggctac cgggctcaga tagacaatct cacccgagaa
5221 aacaggagct tatctgaaga aataaagagg ctgaagaaca ctctaaccca gaccacagag
5281 aatctcagga gggtggaaga agacatccaa cagcaaaagg ccactggctc tgaggtgtct
5341 cagaggaaac agcagctgga ggttgagctg agacaagtca ctcagatgcg aacagaggag
5401 agcgtaagat ataagcaatc tcttgatgat gctgccaaaa ccatccagga taaaaacaag
5461 gagatagaaa ggttaaaaca actgatcgac aaagaaacaa atgaccggaa atgcctggaa
5521 gatgaaaacg cgagattaca aagggtccag tatgacctgc agaaagcaaa cagtagtgcg
5581 acggagacaa taaacaaact gaaggttcag gagcaagaac tgacacgcct gaggatcgac
5641 tatgaaaggg tttcccagga gaggactgtg aaggaccagg atatcacgcg gttccagaac
5701 tctctgaaag agctgcagct gcagaagcag aaggtggaag aggagctgaa tcggctgaag
5761 aggaccgcgt cagaagactc ctgcaagagg aagaagctgg aggaagagct ggaaggcatg
5821 aggaggtcgc tgaaggagca agccatcaaa atcaccaacc tgacccagca gctggagcag
5881 gcatccattg ttaagaagag gagtgaggat gacctccggc agcagaggga cgtgctggat
5941 ggccacctga gggaaaagca gaggacccag gaagagctga ggaggctctc ttctgaggtc
6001 gaggccctga ggcggcagtt actccaggaa caggaaagtg tcaaacaagc tcacttgagg
6061 aatgagcatt tccagaaggc gatagaagat aaaagcagaa gcttaaatga aagcaaaata
6121 gaaattgaga ggctgcagtc tctcacagag aacctgacca aggagcactt gatgttagaa
6181 gaagaactgc ggaacctgag gctggagtac gatgacctga ggagaggacg aagcgaagcg
6241 gacagtgata aaaatgcaac catcttggaa ctaaggagcc agctgcagat cagcaacaac
6301 cggaccctgg aactgcaggg gctgattaat gatttacaga gagagaggga aaatttgaga
6361 caggaaattg agaaattcca aaagcaggct ttagaggcat ctaataggat tcaggaatca
6421 aagaatcagt gtactcaggt ggtacaggaa agagagagcc ttctggtgaa aatcaaagtc
6481 ctggagcaag acaaggcaag gctgcagagg ctggaggatg agctgaatcg tgcaaaatca
6541 actctagagg cagaaaccag ggtgaaacag cgcctggagt gtgagaaaca gcaaattcag
6601 aatgacctga atcagtggaa gactcaatat tcccgcaagg aggaggctat taggaagata
6661 gaatcggaaa gagaaaagag tgagagagag aagaacagtc ttaggagtga gatcgaaaga
6721 ctccaagcag agatcaagag aattgaagag aggtgcaggc gtaagctgga ggattctacc
6781 agggagacac agtcacagtt agaaacagaa cgctcccgat atcagaggga gattgataaa
6841 ctcagacagc gcttaattaa gggaagcggc ggaaccggat cgactagcgg gggtagcggg
6901 ggaagcacgg ggggaggaac tggcgccgtg agcaagggcg aggagaccac aatgggcgta
6961 atcaagcccg acatgaagat caagctgaag atggagggca acgtgaatgg ccacgccttc
7021 gtgatcgagg gcgagggcga gggcaagccc tacgacggca ccaacaccat caacctggag
7081 gtgaaggagg gagcccccct gcccttctcc tacgacattc tgaccaccgc gttcgcctac
7141 ggcaacaggg ccttcaccaa gtaccccgac gacatcccca actacttcaa gcagtccttc
7201 cccgagggct actcttggga gcgcaccatg accttcgagg acaagggcat cgtgaaggtg
7261 aagtccgaca tctccatgga ggaggactcc ttcatctacg agatacacct caagggcgag
7321 aacttccccc ccaacggccc cgtgatgcag aagaagacca ccggctggga cgcctccacc
7381 gagaggatgt acgtgcgcga cggcgtgctg aagggcgacg tcaagcacaa gctgctgctg
7441 gagggcggcg gccaccaccg cgttgacttc aagaccatct acagggccaa gaaggcggtg
7501 aagctgcccg actatcactt tgtggaccac cgcatcgaga tcctgaacca cgacaaggac
7561 tacaacaagg tgaccgttta cgagagcgcc gtggcccgca actccaccga cggcatggac
7621 gagctgtaca aggggccagg tggtgcaggg ccaggtggtg cagggccagg tggtgcaggg
7681 ccaggtggtg cagggcccgg tggtgcaggt ccaggtggtg caggtccagg tggtgcaggt
7741 ccaggtggtg ctatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
7801 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
7861 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
7921 ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc
7981 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
8041 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
8101 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
8161 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
8221 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
8281 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
8341 cccgacaacc actacctgag ctaccagtcc aaactgagca aagaccccaa cgagaagcgc
8401 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
8461 ctgtacaagg gcaccggagg tggcactagc ggaggctcag gcggatcgac ctccggaact
8521 ggaggttccg gtagcggccg ctgaataggt aaggcgcgcc gctagcggcc tgtcaggcca
8581 agcttccatc gatagacatg ataagataca ttgatgagtt tggacaaacc acaacaagaa
8641 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
8701 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
8761 agggggagat gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtacctagg
8821 cgagaccctg tctcacaaaa taaagtaagc ccggactgag tgcggaaagg cgggcctggc
8881 gggtctggtc tccccatgcg ggccaccaga ggccctgcag ccttcagtcg cttgaagggg
8941 taatggcgct tccactcaca aacatggcgg acagagcgtg tgaacgagat gaacagcccc
9001 tcaaaaatat ggccgccgag gctggacggc cgtgccccag cagcaccgcc tccgcgcccc
9061 acgtgatctc tcgccgggca cagcgctgac cgcggaggtc caaccggaag aatgtccgga
9121 ttggacattc ggaagagggc ccgccttccc tggggaatct ctgcgcacgc gcagaacgct
9181 tcgaccaatg aaaacacagg aagccgtccg cgcaaccgcg ttgcgtcact tctgccgccc
9241 ctgtttcaag gtatatagcc gtagacggaa cttcgccttt ctctcggcct tagcgccatt
9301 tttttgggtg agtgtttttt ggttcctgcg ttgggattcc gtgtacaatc catagacatc
9361 tgacctcggc acttagcatc atcacagcaa actaactgta gcctttctct ctttccctgt
9421 agaaacctct gcacctgagg ccaccatgtc tagactggac aagagcaaag tcataaacgg
9481 cgctctggaa ttactcaatg gagtcggtat cgaaggcctg acgacaagga aactcgctca
9541 aaagctggga gttgagcagc ctaccctgta ctggcacgtg aagaacaagc gggccctgct
9601 cgatgccctg ccaatcgaga tgctggacag gcatcatacc cacttctgcc ccctggaagg
9661 cgagtcatgg caagactttc tgcggaacaa cgccaagtca ttccgctgtg ctctcctctc
9721 acatcgcgac ggggctaaag tgcatctcgg cacccgccca acagagaaac agtacgaaac
9781 cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga acgcactgta
9841 cgctctgtcc gccgtgggcc actttacact gggctgcgta ttggaggaac aggagcatca
9901 agtagcaaaa gaggaaagag agacacctac caccgattct atgcccccac ttctgagaca
9961 agcaattgag ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg gcctggaact
10021 aatcatatgt ggcctggaga aacagctaaa gtgcgaaagc ggcgggccgg ccgacgccct
10081 tgacgatttt gacttagaca tgctcccagc cgatgccctt gacgactttg accttgatat
10141 gctgcctgct gacgctcttg acgattttga ccttgacatg ctccccgggt ccggcgctac
10201 taacttcagc ctgctgaagc aggctggtga cgtcgaggag aatcctggtc ccatgaccga
10261 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
10321 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
10381 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
10441 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
10501 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
10561 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
10621 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
10681 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
10741 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
10801 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gattcgaagg
10861 cctgtcgtga agcttgggga tcaattctct agagctcgct gatcagcctc gactgtgcct
10921 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
10981 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
11041 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
11101 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
11161 tgggcctact agctactcgg gaccccttac cgaaacatcg ccgcattctg cagaggagtc
11221 gagtgtatgt aaacttctga cccactggga atgtgatgaa agaaataaaa gctgaaatga
11281 atcattctct ctactattat tctgatattt cacattctta aaataaagtg gtgatcctaa
11341 ctgacctaag acagggaatt tttactagga ttaaatgtca ggaattgtga aaaagtgagt
11401 ttaaatgtat ttggctaagg tgtatgtaaa cttccgactt caactgtata gggatccgct
11461 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
11521 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
11581 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
11641 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
11701 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
11761 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
11821 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
11881 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
11941 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
12001 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
12061 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
12121 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
12181 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
12241 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
12301 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
12361 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
12421 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
12481 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
12541 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
12601 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
12661 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
12721 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
12781 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
12841 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
12901 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
12961 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
13021 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
13081 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
13141 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
13201 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
13261 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
13321 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
13381 cctgatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggaaatt
13441 gtaagcgtta atattttgtt aaaatt
//
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