| 质粒名称: | Flag-HA-USP20 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 22573 |
| 存储实验室: | Wade Harper |
|
载体骨架基本信息 |
|
| 载体名称: | pDEST_LTR_N_FLAG_HA_IRES_puro |
| 载体出品公司: | Harper_Lab |
| 空载体大小: | 6521bp |
| 完整质粒大小: | 9170 bp |
| 载体类型: | 哺乳细胞表达, Retroviral |
| 筛选标记: | Puromycin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | USP20 / ubiquitin specific peptidase 20 /KIAA1003, LSFR3A, VDU2 |
| 物种来源: | H. sapiens (human) |
| 基因突变: | 无 |
| GenBank编号 | NM_006676 |
| 启动子: | CMV |
| 标签/融合蛋白: | HA (N terminal on backbone) |
| 克隆方法: | Gateway Cloning |
| 5’克隆位点: | GATEWAY_LR (unknown if destroyed) |
| 3’克隆位点: | GATEWAY_LR (unknown if destroyed) |
| 5’测序引物 | CAGCCCTCACTCCTTCTCTAGG |
| 3’测序引物 | CAAGCGGCTTCGGCCAGTAAC |
|
文献引用方法 |
|
| 材料和方法部分: |
Flag-HA-USP20 was a gift from Wade Harper (Addgene plasmid # 22573 ; http://n2t.net/addgene:22573 ; RRID:Addgene_22573)
|
| 参考文献部分: | Defining the human deubiquitinating enzyme interaction landscape. Sowa ME, Bennett EJ, Gygi SP, Harper JW. Cell. 2009 Jul 23. 138(2):389-403. 10.1016/j.cell.2009.04.042 PubMed 19615732 |
买家导航
Flag-HA-USP20载体质粒基本信息
Flag-HA-USP20质粒图谱载体图谱和Flag-HA-USP20载体序列质粒序列多克隆位点信息
Flag-HA-USP20质粒载体简介
Flag-HA-USP20质粒序列载体序列
LOCUS Flag-HA-USP20 9170 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Flag-HA-USP20
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9170)
AUTHORS Sowa ME, Bennett EJ, Gygi SP, Harper JW
TITLE Defining the human deubiquitinating enzyme interaction landscape.
JOURNAL Cell. 2009 Jul 23. 138(2):389-403.
PUBMED 19615732
REFERENCE 2 (bases 1 to 9170)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9170
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(63..80)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(234..822)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(314..333)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(993..1853)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 1616..1635
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(1854..1958)
/gene="bla"
/label=AmpR promoter
primer_bind 2026..2044
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(2082..2104)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 2204..2223
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 2417..2439
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 2567..2586
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
LTR 2786..3302
/label=5' LTR
/note="5' long terminal repeat from murine embryonic stem
cell virus"
misc_feature 3366..3707
/label=MESV Psi
/note="packaging signal of murine embryonic stem cell
virus"
CDS 3774..4190
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 4120..4142
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 4158..4174
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
regulatory 4203..4212
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 4212..4235
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
CDS 4248..4274
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
protein_bind 4290..4314
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
protein_bind complement(7057..7081)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
misc_feature 7111..7663
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(7255..7272)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 7482..7501
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 7675..8274
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(7675..7694)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 8171..8191
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
LTR 8350..8864
/label=3' LTR
/note="3' long terminal repeat from murine embryonic stem
cell virus"
protein_bind 9026..9042
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(9050..9080)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 9095..9116
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
ORIGIN
1 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
61 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
121 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
181 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
241 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
301 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
361 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
421 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
481 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
541 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
601 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
661 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
721 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
781 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
841 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
901 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
961 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
1021 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
1081 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
1141 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
1201 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
1261 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
1321 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
1381 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
1441 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
1501 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
1561 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
1621 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
1681 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
1741 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
1801 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
1861 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
1921 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
1981 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
2041 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
2101 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
2161 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
2221 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
2281 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
2341 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
2401 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggcgcaagg aatggtgcat
2461 gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga
2521 aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga
2581 tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt
2641 agaggcgatt agtccaattt gttaaagaca ggatatcagt ggtccaggct ctagttttga
2701 ctcaacaata tcaccagctg aagcctatag agtacgagcc atagataaaa taaaagattt
2761 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag
2821 cttaagtaac gccattttgc aaggcatgga aaatacataa ctgagaatag agaagttcag
2881 atcaaggtta ggaacagaga gacagcagaa tatgggccaa acaggatatc tgtggtaagc
2941 agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc ccgccctcag
3001 cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt
3061 gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc
3121 cgagctcaat aaaagagccc acaacccctc actcggcgcg ccagtcctcc gatagactgc
3181 gtcgcccggg tacccgtatt cccaataaag cctcttgctg tttgcatccg aatcgtggac
3241 tcgctgatcc ttgggagggt ctcctcagat tgattgactg cccacctcgg gggtctttca
3301 tttggaggtt ccaccgagat ttggagaccc ctgcccaggg accaccgacc cccccgccgg
3361 gaggtaagct ggccagcggt cgtttcgtgt ctgtctctgt ctttgtgcgt gtttgtgccg
3421 gcatctaatg tttgcgcctg cgtctgtact agttagctaa ctagctctgt atctggcgga
3481 cccgtggtgg aactgacgag ttctgaacac ccggccgcaa ccctgggaga cgtcccaggg
3541 actttggggg ccgtttttgt ggcccgacct gaggaaggga gtcgatgtgg aatccgaccc
3601 cgtcaggata tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg
3661 aatttttgct ttcggtttgg aaccgaagcc gcgcgtcttg tctgctgcag cgctgcagca
3721 tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt gtctgaaaat tagggccaga
3781 ctgttaccac tcccttaagt ttgaccttag gtcactggaa agatgtcgag cggatcgctc
3841 acaaccagtc ggtagatgtc aagaagagac gttgggttac cttctgctct gcagaatggc
3901 caacctttaa cgtcggatgg ccgcgagacg gcacctttaa ccgagacctc atcacccagg
3961 ttaagatcaa ggtcttttca cctggcccgc atggacaccc agaccaggtc ccctacatcg
4021 tgacctggga agccttggct tttgaccccc ctccctgggt caagcccttt gtacacccta
4081 agcctccgcc tcctcttcct ccatccgccc cgtctctccc ccttgaacct cctcgttcga
4141 ccccgcctcg atcctccctt tatccagccc tcactccttc tctaggcgcc ggaattagat
4201 ctgccaccat ggactacaag gatgacgatg acaagctcga tggaggatac ccatacgatg
4261 ttccagatta cgctgctcga ggttatcaaa caagtttgta caaaaaagtt ggcatggggg
4321 actccaggga cctttgccct caccttgact ccataggaga ggtgaccaaa gaggacttgc
4381 tgctcaaatc taagggaacc tgtcagtcgt gtggggtcac cggaccaaac ctatgggcct
4441 gtctgcaggt tgcctgcccc tatgttggct gcggagaatc cttcgctgac cacagcacca
4501 ttcatgcaca ggcaaaaaag cacaacttga ccgtgaacct gaccacgttc cgactgtggt
4561 gttacgcctg tgagaaggag gtattcctgg agcagcggct ggcagcccct ctgctgggct
4621 cctcttccaa gttctctgaa caggactccc cgccaccctc ccaccctctg aaagctgttc
4681 ctattgctgt ggctgatgaa ggagagtctg agtcagagga tgatgacctg aaacctcgag
4741 gcctcacggg catgaagaac ctcgggaact cctgctacat gaacgccgcc ctgcaggccc
4801 tgtccaattg cccgccgctg actcagttct tcttggagtg tggcggcctg gtgcgcacag
4861 ataagaagcc agccctgtgc aagagctacc agaagctggt ctctgaggtc tggcataaga
4921 aacggccaag ctacgtggtc cccaccagtc tgtctcatgg gatcaagttg gtcaacccaa
4981 tgttccgagg ctatgcccag caggacaccc aagagttcct tcgctgcctg atggaccagc
5041 tgcacgagga gctcaaggag ccggtggtgg ccacggtggc gctgacggag gctcgggact
5101 cagattcgag tgacacggat gagaaacggg agggtgaccg gagcccatca gaagatgagt
5161 tcttgtcctg tgactcgagc agtgaccggg gtgagggtga cgggcagggg cgtggcgggg
5221 gcagctcgca ggccgagacg gagctgctga tcccagatga ggcgggccga gccatctctg
5281 agaaggagcg gatgaaggac cgcaagttct cctggggcca gcagcgtaca aactcggagc
5341 aagtggacga ggacgctgat gtggacactg ccatggctgc ccttgaccag cccgcggagg
5401 cccagccccc gtcaccacgg tcctccagcc cctgccggac gccagagccg gacaatgatg
5461 ctcacctacg cagctcctct cgcccctgca gccccgtcca ccaccacgag ggccatgcca
5521 agctgtctag cagcccccct cgtgcaagcc ccgtgaggat ggcaccgtcg tacgtgctca
5581 agaaagccca ggtattgagt gctggcagcc ggaggcggaa ggagcagcgc taccgcagcg
5641 tcatctcaga catctttgac ggctccattc tcagcctcgt gcagtgtctc acctgtgacc
5701 gggtatccac cacagtggaa acgttccagg acttatcact gcccattcct ggaaaggagg
5761 acctggccaa gctccattca gccatctacc agaatgtgcc ggccaagcca ggcgcctgtg
5821 gggacagcta tgccgcccag ggctggctgg ccttcattgt ggagtacatc cgacggtttg
5881 tggtatcctg tacccccagc tggttttggg ggcctgtcgt caccctggaa gactgccttg
5941 ctgccttctt tgccgctgat gagttaaagg gtgacaacat gtacagctgt gagcggtgta
6001 agaagctgcg gaacggagtg aagtactgca aagtcctgcg gttgcccgag atcctgtgca
6061 ttcacctaaa gcgctttcgg cacgaggtga tgtactcatt caagatcaac agccacgtct
6121 ccttccccct cgaggggctc gacctgcgcc ccttccttgc caaggagtgc acatcccaga
6181 tcaccaccta cgacctcctc tcggtcatct gccaccacgg cacggcaggc agtgggcact
6241 acatcgccta ctgccagaac gtgatcaatg ggcagtggta cgagtttgat gaccagtacg
6301 tcacagaagt ccacgagacg gtggtgcaga acgccgaggg ctacgtactc ttctacagga
6361 agagcagcga ggaggccatg cgggagcgac agcaggtggt gtccctggcc gccatgcggg
6421 agcccagcct gctgcggttc tacgtgtccc gcgagtggct caacaagttc aacaccttcg
6481 cggagccagg ccccatcacc aaccagacct tcctctgctc ccacggaggc atcccgcccc
6541 acaaatacca ctacatcgac gacctggtgg tcatcctgcc ccagaacgtc tgggagcacc
6601 tgtacaacag attcgggggt ggccccgccg tgaaccacct gtacgtgtgc tccatctgcc
6661 aggtggagat cgaggcactg gccaagcgca ggaggatcga gatcgacacc ttcatcaagt
6721 tgaacaaggc cttccaggcc gaggagtcgc cgggcgtcat ctactgcatc agcatgcagt
6781 ggttccggga gtgggaggcg ttcgtcaagg ggaaggacaa cgagcccccc gggcccattg
6841 acaacagcag gattgcacag gtcaaaggaa gcggccatgt ccagctgaag cagggagctg
6901 actacgggca gatttcggag gagacctgga cctacctgaa cagcctgtat ggaggtggcc
6961 ccgagattgc catccgccag agtgtggcgc agccgctggg cccagagaac ctgcacgggg
7021 agcagaagat cgaagccgag acgcgggccg tgtgataccc aactttcttg tacaaagtgg
7081 ttcgataacg aattccgccc ccccccccta acgttactgg ccgaagccgc ttggaataag
7141 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
7201 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
7261 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
7321 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
7381 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
7441 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
7501 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
7561 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
7621 accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac aaccatgacc
7681 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
7741 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
7801 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
7861 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
7921 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
7981 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
8041 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
8101 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
8161 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
8221 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgatgcatc
8281 gatgtcgacc tgcagccaag cttatcgata aaataaaaga ttttatttag tctccagaaa
8341 aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt
8401 tgcaaggcat ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag
8461 agagacagca gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc
8521 agggccaaga acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca
8581 tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac
8641 caatcagttc gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag
8701 cccacaaccc ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt
8761 gtatccaata aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg
8821 gtctcctctg agtgattgac tacccgtcag cgggggtctt tcatgggtaa cagtttcttg
8881 aagttggaga acaacattct gagggtagga gtcgaatatt aagtaatcct gactcaatta
8941 gccactgttt tgaatccaca tactccaata ctcctgaaat agttcattat ggacagcgca
9001 gaaagagctg gggagaattg tgaaattgtt atccgctcac aattccacac aacatacgag
9061 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
9121 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
//
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Flag-HA-USP20质粒载体应用举例
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