pAAV-UbC

价格:1100元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Addgene
载体名称: pAAV-UbC
质粒类型: 腺病毒载体;RNAi载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: UbC
载体大小: 5185 bp
5' 测序引物及序列: pCAG fwd primer 5’CAACGTGCTGGTTATTGTG 3’
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素
筛选标记: --
克隆菌株: Stbl3 或 NEB Stble
宿主细胞(系): 常规哺乳动物细胞系
备注: --
产品目录号: #62806
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 腺病毒

载体质粒图谱和多克隆位点信息

pAAV-Ubc载体图谱



pAAV-Ubc 多克隆位点

载体简介

载体序列

LOCUS       pAAV-UbC	5185 bp 	DNA	SYN
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTNAME|pAAV-UbC|
FEATURES             Location/Qualifiers
     source          1..5185
                     /organism="pAAV-UbC"
                     /mol_type="other DNA"
     promoter        157..1282
                     /label="hUbC_promoter"
     misc_feature    1312..1827
                     /label="bGlob_int"
     misc_feature    1835..1854
                     /label="pCAG_F_primer"
     terminator      1930..2408
                     /label="hGH_PA_terminator"
     rep_origin      2680..2986
                     /label="f1_origin"
     misc_feature    3254..3276
                     /label="pGEX_3_primer"
     promoter        3435..3463
                     /label="AmpR_promoter"
     gene            3505..4365
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             3505..4365
                     /label="ORF frame 1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      4520..5139
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCAAAG CCCGGGCGTC
   61 GGGCGACCTT TGGTCGCCCG GCCTCAGTGA GCGAGCGAGC GCGCAGAGAG GGAGTGGCCA
  121 ACTCCATCAC TAGGGGTTCC TGCGGCCGCG GCCTCCGCGC CGGGTTTTGG CGCCTCCCGC
  181 GGGCGCCCCC CTCCTCACGG CGAGCGCTGC CACGTCAGAC GAAGGGCGCA GCGAGCGTCC
  241 TGATCCTTCC GCCCGGACGC TCAGGACAGC GGCCCGCTGC TCATAAGACT CGGCCTTAGA
  301 ACCCCAGTAT CAGCAGAAGG ACATTTTAGG ACGGGACTTG GGTGACTCTA GGGCACTGGT
  361 TTTCTTTCCA GAGAGCGGAA CAGGCGAGGA AAAGTAGTCC CTTCTCGGCG ATTCTGCGGA
  421 GGGATCTCCG TGGGGCGGTG AACGCCGATG ATTATATAAG GACGCGCCGG GTGTGGCACA
  481 GCTAGTTCCG TCGCAGCCGG GATTTGGGTC GCGGTTCTTG TTTGTGGATC GCTGTGATCG
  541 TCACTTGGTG AGTAGCGGGC TGCTGGGCTG GCCGGGGCTT TCGTGGCCGC CGGGCCGCTC
  601 GGTGGGACGG AAGCGTGTGG AGAGACCGCC AAGGGCTGTA GTCTGGGTCC GCGAGCAAGG
  661 TTGCCCTGAA CTGGGGGTTG GGGGGAGCGC AGCAAAATGG CGGCTGTTCC CGAGTCTTGA
  721 ATAGAACCTT CGCTAATGCG GGAAAGCTCT TATTCGGGTG AGATGGGCTG GGGCACCATC
  781 TGGGGACCCT GACGTGAAGT TTGTCACTGA CTGGAGAACT CGGTTTGTCG TCTGTTGCGG
  841 GGGCGGCAGT TATGGCGGTG CCGTTGGGCA GTGCACCCGT ACCTTTGGGA GCGCGCGCCC
  901 TCGTCGTGTC GTGACGTCAC CCGTTCTGTT GGCTTATAAT GCAGGGTGGG GCCACCTGCC
  961 GGTAGGTGTG CGGTAGGCTT TTCTCCGTCG CAGGACGCAG GGTTCGGGCC TAGGGTAGGC
 1021 TCTCCTGAAT CGACAGGCGC CGGACCTCTG GTGAGGGGAG GGATAAGTGA GGCGTCAGTT
 1081 TCTTTGGTCG GTTTTATGTA CCTATCTTCT TAAGTAGCTG AAGCTCCGGT TTTGAACTAT
 1141 GCGCTCGGGG TTGGCGAGTG TGTTTTGTGA AGTTTTTTAG GCACCTTTTG AAATGTAATC
 1201 ATTTGGGTCA ATATGTAATT TTCAGTGTTA GACTAGTAAA TTGTCCGCTA AATTCTGGCC
 1261 GTTTTTGGCT TTTTTGTTAG ACGAAGCTAA GGCGCGCCTG AGAACTTCAG GGTGAGTTTG
 1321 GGGACCCTTG ATTGTTCTTT CTTTTTCGCT ATTGTAAAAT TCATGTTATA TGGAGGGGGC
 1381 AAAGTTTTCA GGGTGTTGTT TAGAATGGGA AGATGTCCCT TGTATCACCA TGCATGGACC
 1441 CTCATGATAA TTTTGTTTCT TTCACTTTCT ACTCTGTTGA CAACCATTGT CTCCTCTTAT
 1501 TTTCTTTTCA TTTTCTGTAA CTTTTTCGTT AAACTTTAGC TTGCATTTGT AACGAATCTA
 1561 GACAATCAGG GTATATTATA TTGTACTTCA GCACAGTTTT AGAGAACAAT TGTTATAATT
 1621 AAATGATAAG GTAGAATATT TCTGCATATA AATTCTGGCT GGCGTGGAAA TATTCTTATT
 1681 GGTAGAAACA ACTACATCCT GGTCATCATC CTGCCTTTCT CTTTATGGTT ACAATGATAT
 1741 ACACTGTTTG AGATGAGGAT AAAATACTCT GAGTCCAAAC CGGGCCCCTC TGCTAACCAT
 1801 GTTCATGCCT TCTTCTTTTT CCTACAGCTC CTGGGCAACG TGCTGGTTAT TGTGCTGTCT
 1861 CATCAAGCTT GGATCCGAAT TCGCTAGCGA TATCTCGAGT CTAGAGTTAA CGGCCATATT
 1921 GGCCAATCTA CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG
 1981 TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG
 2041 ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG
 2101 TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG
 2161 CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC
 2221 CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT
 2281 GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA
 2341 TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC
 2401 CTGTCCTTCT GATTTTGTAG GTAACCACGT GCGGACCGAG CGGCCGCAGG AACCCCTAGT
 2461 GATGGAGTTG GCCACTCCCT CTCTGCGCGC TCGCTCGCTC ACTGAGGCCG GGCGACCAAA
 2521 GGTCGCCCGA CGCCCGGGCT TTGCCCGGGC GGCCTCAGTG AGCGAGCGAG CGCGCAGCTG
 2581 CCTGCAGGGG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG
 2641 CATACGTCAA AGCAACCATA GTACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG
 2701 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT
 2761 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG
 2821 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTTG
 2881 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG
 2941 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC
 3001 TCGGGCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT
 3061 GAGCTGATTT AACAAAAATT TAACGCGAAT TTTAACAAAA TATTAACGTT TACAATTTTA
 3121 TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGCC CCGACACCCG
 3181 CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA
 3241 GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC
 3301 GCGAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG
 3361 GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA
 3421 TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT
 3481 CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC
 3541 TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA
 3601 GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT
 3661 AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT
 3721 CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC
 3781 ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG
 3841 GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG
 3901 GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC
 3961 ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA
 4021 AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA
 4081 ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT
 4141 AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA
 4201 TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG
 4261 CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT
 4321 AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT
 4381 TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG
 4441 AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA
 4501 GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA
 4561 ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA
 4621 GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT
 4681 GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA
 4741 TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT
 4801 ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG
 4861 GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG
 4921 CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA
 4981 AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT
 5041 CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG
 5101 TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC
 5161 TTTTGCTGGC CTTTTGCTCA CATGT
//

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