| 质粒名称: | pXSE901BG |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 91714 |
| 存储实验室: | Qi-Jun Chen |
|
载体骨架基本信息 |
|
| 载体名称: | pCambia |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 植物表达 |
| 筛选标记: | Basta ; Glyphosate |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pXSE901BG was a gift from Qi-Jun Chen (Addgene plasmid # 91714 ; http://n2t.net/addgene:91714 ; RRID:Addgene_91714) |
| 参考文献部分: | CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis. Chen Y, Wang Z, Ni H, Xu Y, Chen Q, Jiang L. Sci China Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5. 10.1007/s11427-017-9021-5 [pii] PubMed 28303459 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pXSE901BG 17520 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A)
under CaMV 35S promoter and empty gRNA scaffold, Basta and
glyphosate-resistance.
ACCESSION .
VERSION .
KEYWORDS pXSE901BG
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 17520)
AUTHORS Chen Y, Wang Z, Ni H, Xu Y, Chen Q, Jiang L
TITLE CRISPR/Cas9-mediated base-editing system efficiently generates
gain-of-function mutations in Arabidopsis.
JOURNAL Sci China Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5.
PUBMED 28303459
REFERENCE 2 (bases 1 to 17520)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..17520
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 1..25
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
primer_bind 213..235
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 227..244
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 228..244
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
CDS 957..1748
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
misc_RNA 1900..1975
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
promoter 2589..2933
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
CDS 3083..3769
/codon_start=1
/product="cytidine deaminase (C to U editing enzyme) from
rat"
/label=APOBEC-1
/note="can use ssDNA as a substrate (Komor et al., 2016)"
/translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
LWATGLK"
CDS 3818..7918
/codon_start=1
/product="nickase mutant of the Cas9 endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system"
/label=Cas9(D10A)
/note="generates RNA-guided single strand nicks in DNA due
to the D10A mutation in the RuvC catalytic domain"
/translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
RBS 5601..5609
/label=Shine-Dalgarno sequence
/note="full consensus sequence for ribosome-binding sites
upstream of start codons in E. coli; complementary to a
region in the 3' end of the 16S rRNA (Chen et al., 1994)"
CDS 7931..8179
/codon_start=1
/product="uracil-DNA glycosylase inhibitor from a Bacillus
subtilis bacteriophage (Mol et al., 1995)"
/label=UGI
/translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
CDS 8192..8212
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
promoter 9294..9638
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
misc_feature 11241..11265
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
CDS 11690..12484
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
rep_origin 12571..13159
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 13060..13079
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 13313..13330
/label=L4440
/note="L4440 vector, forward primer"
misc_feature 13345..13485
/label=bom
/note="basis of mobility region from pBR322"
primer_bind complement(13411..13430)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 13571..13593
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
rep_origin 13829..14023
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
CDS complement(14089..15162)
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
CDS complement(15591..16220)
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
61 aatctgatcc aagctcaagc tgctctagca ttcgccattc aggctgcgca actgttggga
121 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
181 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
241 cagtgccaag cttcgacttg ccttccgcac aatacatcat ttcttcttag ctttttttct
301 tcttcttcgt tcatacagtt tttttttgtt tatcagctta cattttcttg aaccgtagct
361 ttcgttttct tctttttaac tttccattcg gagtttttgt atcttgtttc atagtttgtc
421 ccaggattag aatgattagg catcgaacct tcaagaattt gattgaataa aacatcttca
481 ttcttaagat atgaagataa tcttcaaaag gcccctggga atctgaaaga agagaagcag
541 gcccatttat atgggaaaga acaatagtat ttcttatata ggcccattta agttgaaaac
601 aatcttcaaa agtcccacat cgcttagata agaaaacgaa gctgagttta tatacagcta
661 gagtcgaagt agtgattggg agaccaaccc agtggacata agcctgttcg gttcgtaagc
721 tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
781 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt
841 ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat
901 ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatgg
961 gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc
1021 gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc
1081 tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa
1141 cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga
1201 ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc
1261 cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct
1321 tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata
1381 gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc
1441 tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg
1501 atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa
1561 tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc
1621 ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc
1681 gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg
1741 gcaaataatg tctagctaga aattcgttca agccgacgcc gcttcgcggc gcggcttaac
1801 tcaagcgtta gatgcactaa gcacataatt gctcacagcc aaactatcag gtcaagtctg
1861 cttttattat ttttaagcgt gcataataag ccggtctcgg ttttagagct agaaatagca
1921 agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt
1981 tttgcaaaat tttccagatc gatttcttct tcctctgttc ttcggcgttc aatttctggg
2041 gttttctctt cgttttctgt aactgaaacc taaaatttga cctaaaaaaa atctcaaata
2101 atatgattca gtggttttgt acttttcagt tagttgagtt ttgcagttcc gatgagataa
2161 accaataagc ttgcatgcct gcaggtcaac atggtggagc acgacacact tgtctactcc
2221 aaaaatatca aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg
2281 gtaatatccg gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag
2341 atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc
2401 gttgaagatg cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc
2461 gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgataacatg
2521 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
2581 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
2641 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
2701 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
2761 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
2821 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
2881 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag gacctcgacc
2941 tcaacacaac atatacaaaa caaacgaatc tcaagcaatc aagcattcta cttctattgc
3001 agcaatttaa atcatttctt ttaaagcaaa agcaattttc tgaaaatttt caccatttac
3061 gaacgatact cgagtaatct agatgtcttc cgagacagga ccggttgccg tcgaccctac
3121 tcttagaagg cgcattgagc cacacgagtt cgaggtgttc ttcgatccga gagagctgag
3181 gaaggagact tgcctccttt acgagatcaa ttggggcgga aggcactcta tttggcgcca
3241 tacctcccag aacacaaata agcatgtgga ggttaatttc atcgagaagt tcactaccga
3301 gaggtacttc tgcccaaaca cacgctgcag catcacttgg ttccttagct ggtcaccgtg
3361 cggagagtgc tctcgcgcca ttacagagtt cctgtccaga tacccgcacg ttactctttt
3421 catctacatt gccagactgt accaccatgc ggatcctcgc aacagacagg gtcttaggga
3481 cctgatcagc tcaggcgtca ccatccagat tatgacagag caggagtctg gatactgctg
3541 gcgcaacttc gtgaattact ctccttccaa tgaggctcac tggccaagat acccgcatct
3601 gtgggtcagg ctctacgtgc tcgagcttta ctgcatcatt cttggtctgc ctccatgcct
3661 caacatcctt agaaggaagc agccacagct cacattcttc actattgccc ttcagtcttg
3721 ccactaccag aggcttccgc ctcatattct gtgggcgact ggcctcaaga gcggctcaga
3781 gactccggga acatctgagt ccgctactcc tgagtctgac aagaagtact ccatcggact
3841 cgccattggt actaactccg ttggatgggc ggtcatcacc gatgagtaca aggtgcctag
3901 caagaagttc aaggttcttg gtaacacaga cagacactca atcaagaaga atctgattgg
3961 tgctctgctc ttcgattctg gagagactgc cgaggctacc aggctcaaga gaaccgcccg
4021 cagaaggtac acacgcagaa agaataggat ctgctacctt caggagattt tctctaacga
4081 gatggctaag gttgatgaca gcttcttcca tcgccttgag gagtcattcc tggtcgagga
4141 ggacaagaag cacgagagac atcctatctt cggtaacatt gtcgatgagg tggcctacca
4201 cgagaagtac ccaactatct accatcttag gaagaagctg gtggatagca ccgacaaggc
4261 ggatctccgc cttatctacc tggctctcgc ccacatgatt aagttcagag gccatttcct
4321 catcgagggc gatctcaacc cagataattc agacgtcgat aagctcttca tccagcttgt
4381 gcagacatac aatcagcttt tcgaggagaa cccgattaat gcgagcggtg ttgatgcgaa
4441 ggctatcctg tcagctagac tcagcaagtc aaggcgcctg gagaacctca tcgcccagct
4501 gccaggcgag aagaagaacg gtcttttcgg caatctgatt gcgctttctc tgggactcac
4561 cccgaacttc aagtccaatt tcgacctggc tgaggatgcc aagctccagc tgtctaagga
4621 tacatacgat gacgatctcg acaaccttct ggctcagatc ggcgaccagt acgccgatct
4681 cttccttgct gccaagaatc ttagcgatgc catcctcctt tcagacattc tgagagttaa
4741 cactgagatt accaaggctc cgctgtctgc ctccatgatc aagagatacg atgagcacca
4801 tcaggacctc actctgctca aggcgctggt ccgccagcag ctccctgaga agtacaagga
4861 gatcttcttc gaccagtcta agaacggcta cgcgggttac attgatggtg gcgctagcca
4921 ggaggagttc tacaagttca tcaagccaat tctggagaag atggatggca ctgaggagct
4981 tctggtcaag ctcaataggg aggatctcct taggaagcag cgcaccttcg acaacggatc
5041 tatccctcac cagattcatc ttggagagct gcacgccatc ctcagaaggc aggaggattt
5101 ctacccattc cttaaggaca accgcgagaa gatcgagaag attctgactt tcagaatccc
5161 ttactacgtt ggcccgctcg ctagaggcaa ctctaggttc gcgtggatga ccaggaagtc
5221 agaggagact atcacccctt ggaacttcga ggaggtggtt gacaagggag ccagcgcgca
5281 gtcattcatt gagcgcatga ctaatttcga taagaacctg cctaatgaga aggtcctccc
5341 aaagcatagc ctgctctacg agtacttcac tgtgtacaac gagcttacca aggtgaagta
5401 tgtgacagag ggcatgcgca agccggcttt cctttcagga gagcagaaga aggccatcgt
5461 ggatcttctg ttcaagacta atagaaaggt caccgtgaag cagctgaagg aggattactt
5521 caagaagatt gagtgcttcg actctgttga gatctccggt gtcgaggata ggttcaacgc
5581 ttccctcggc acctaccacg acctccttaa gatcattaag gacaaggatt tcctggataa
5641 cgaggagaat gaggacatcc tcgaggatat tgtgctgaca ctcactcttt tcgaggacag
5701 ggagatgatc gaggagcgcc ttaagacata cgcgcatctg ttcgacgata aggttatgaa
5761 gcagctcaag cgcagaaggt acactggatg gggtagactc tctaggaagc tcatcaacgg
5821 catcagagat aagcagtctg gcaagactat tctcgatttc cttaagtccg acggcttcgc
5881 taacaggaat ttcatgcagc tcattcacga cgattctctt actttcaagg aggacatcca
5941 gaaggcgcag gttagcggcc agggagattc actgcacgag catatcgcga acctcgctgg
6001 ctcccctgct atcaagaagg gcatcctcca gaccgttaag gtcgtggatg agctggttaa
6061 ggtcatgggc agacataagc cagagaacat cgtcattgag atggccaggg agaatcagac
6121 aactcagaag ggacagaaga actctaggga gcgcatgaag agaatcgagg agggtattaa
6181 ggagcttggc tcccagatcc tgaaggagca cccggtggag aacacacagc tgcagaatga
6241 gaagctgtac ctctactacc tccagaatgg ccgcgacatg tatgtggatc aggagcttga
6301 cattaacaga ctttctgact acgatgtgga ccatatcgtt ccacagtctt tccttaagga
6361 cgattccatt gataataagg tgctgactag atccgataag aacaggggaa agtctgacaa
6421 tgttccgtcc gaggaggttg tcaagaagat gaagaactac tggaggcagc tgctcaatgc
6481 taagctcatc acccagagga agttcgacaa ccttacaaag gccgagcgcg gaggtctgag
6541 cgagcttgat aaggcgggtt tcattaagag acagctcgtt gagacaaggc agatcactaa
6601 gcacgtcgcc cagattcttg actcaaggat gaacaccaag tacgacgaga atgataagct
6661 gatccgcgag gtgaaggtta ttacactgaa gagcaagctc gtttcagatt tcagaaagga
6721 cttccagttc tacaaggtca gggagatcaa caattaccac catgcccatg atgcgtacct
6781 caacgcggtg gttggtactg ctcttattaa gaagtacccg aagctggagt ctgagttcgt
6841 gtacggcgat tacaaggtgt acgacgttag aaagatgatc gctaagagcg agcaggagat
6901 tggcaaggct accgccaagt acttcttcta ctcaaacatt atgaatttct tcaagacaga
6961 gatcactctc gcgaacggcg agatcagaaa gaggccactt attgagacta acggcgagac
7021 aggagagatc gtctgggata agggtcgcga cttcgctact gtcagaaagg tgctctctat
7081 gccgcaggtt aatattgtca agaagactga ggtgcagacc ggcggattct ctaaggagtc
7141 cattctccct aagaggaact ccgacaagct catcgcccgc aagaaggatt gggaccctaa
7201 gaagtacggt ggcttcgata gcccaaccgt cgcttactca gtgcttgtcg tggccaaggt
7261 cgagaaggga aagagcaaga agctgaagtc agtgaaggag cttctgggta tcacaattat
7321 ggagaggtct tccttcgaga agaatcctat cgacttcctc gaggcgaagg gctacaagga
7381 ggttaagaag gatcttatca ttaagctgcc aaagtactca cttttcgagc tggagaacgg
7441 acgcaagaga atgctggcgt ctgctggaga gcttcagaag ggtaatgagc ttgctctgcc
7501 gtctaagtat gtgaacttcc tctaccttgc ctctcattac gagaagctca agggctcccc
7561 tgaggacaac gagcagaagc agctgttcgt cgagcagcac aagcattacc tcgatgagat
7621 cattgagcag attagcgagt tctcaaagag agtgatcctc gccgatgcga atctcgacaa
7681 ggttcttagc gcgtacaaca agcaccgcga taagccaatc agagagcagg ctgagaatat
7741 cattcatctc ttcaccctta caaacctggg tgctccggcg gctttcaagt acttcgatac
7801 cacaattgac aggaagcgct acacttcaac caaggaggtg ctggacgcca ccctcatcca
7861 ccagtctatt actggcctct acgagactag gatcgatctc tcccagcttg gtggtgactc
7921 tggcggatcc accaacctca gcgatatcat tgagaaggag acaggcaagc agcttgttat
7981 ccaggagtca attctgatgc tcccggagga ggtggaggag gttattggca ataagcctga
8041 gtctgatatc ctcgtgcata ctgcctacga tgagagcacc gacgagaacg ttatgctcct
8101 tacatcagac gcgcctgagt acaagccttg ggctctcgtc attcaggatt ccaacggaga
8161 gaataagatc aagatgctta gcggtggctc tcctaagaag aagagaaagg tgtgagctca
8221 gagctttcgt tcgtatcatc ggtttcgaca acgttcgtca agttcaatgc atcagtttca
8281 ttgcgcacac accagaatcc tactgagttt gagtattatg gcattgggaa aactgttttt
8341 cttgtaccat ttgttgtgct tgtaatttac tgtgtttttt attcggtttt cgctatcgaa
8401 ctgtgaaatg gaaatggatg gagaagagtt aatgaatgat atggtccttt tgttcattct
8461 caaattaata ttatttgttt tttctcttat ttgttgtgtg ttgaatttga aattataaga
8521 gatatgcaaa cattttgttt tgagtaaaaa tgtgtcaaat cgtggcctct aatgaccgaa
8581 gttaatatga ggagtaaaac acttgtagtt gtaccattat gcttattcac taggcaacaa
8641 atatattttc agacctagaa aagctgcaaa tgttactgaa tacaagtatg tcctcttgtg
8701 ttttagacat ttatgaactt tcctttatgt aattttccag aatccttgtc agattctaat
8761 cattgcttta taattatagt tatactcatg gatttgtagt tgagtatgaa aatatttttt
8821 aatgcatttt atgacttgcc aattgattga caacgaattc gaggcggttt gcgtattggc
8881 tagagcagct tgccaacatg gtggagcacg acactctcgt ctactccaag aatatcaaag
8941 atacagtctc agaagaccaa agggctattg agacttttca acaaagggta atatcgggaa
9001 acctcctcgg attccattgc ccagctatct gtcacttcat caaaaggaca gtagaaaagg
9061 aaggtggcac ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt caagatgcct
9121 ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag
9181 acgttccaac cacgtcttca aagcaagtgg attgatgtga acatggtgga gcacgacact
9241 ctcgtctact ccaagaatat caaagataca gtctcagaag accaaagggc tattgagact
9301 tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac
9361 ttcatcaaaa ggacagtaga aaaggaaggt ggcacctaca aatgccatca ttgcgataaa
9421 ggaaaggcta tcgttcaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc
9481 acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga
9541 tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct
9601 tcctctatat aaggaagttc atttcatttg gagaggacct cgacaccatc ttccacacac
9661 tcaagccaca ctattggaga acacacaggg acaacacacc ataagatcca agggaggcca
9721 ccgccgccgc gccggtaacc accccgcccc tctcctcttt ctttctccgt ttgatctttc
9781 tttcttcttt ttgtgggtag aatttgaatc cctcagcatt gttcatcggt agtttttctt
9841 ttcatgattt gtgacaaatg cagcctcgtg cggagctttt ttgtaggtag aagctagatg
9901 agccccgaga ggaggccagc ggacattagg agggcgactg aggcggatat gccagccgtc
9961 tgcaccatcg tcaatcacta catcgaaacc tccacggtga acttcaggac agagccgcag
10021 gagccccagg agtggactga cgatctcgtc cgcctgaggg agcgctaccc gtggctcgtc
10081 gctgaggttg acggcgaggt ggctgggatc gcttacgctg gcccctggaa ggcgcgcaat
10141 gcttacgatt ggaccgccga gagcacggtt tacgtgtcgc caaggcacca gaggaccggc
10201 ctcgggtcaa cactgtacac tcatctcctg aagtccctgg aggctcaggg cttcaagagc
10261 gtggtcgccg ttattggcct gccaaacgac ccttctgtgc ggatgcacga ggccctcggc
10321 tacgctccac gcgggatgct gagggctgct ggcttcaagc acgggaattg gcatgacgtg
10381 ggcttctggc agctcgattt ctcgctgccg gtcccgccga ggccggtgct ccccgtcacg
10441 gagatcctga acttcgacct cctgaagctg gccggcgatg tcgagtctaa cccagggcct
10501 atgatcgagg ttaagccgat taatgcggag gacacctacg atctccgcca cagggtcctg
10561 cgcccaaatc agcctatcga ggcgtgcatg ttcgagtctg acctcacgcg ctcagcgttc
10621 catctgggcg ggttctacgg cgggaagctc atttcagtgg cgtccttcca ccaggctgag
10681 catagcgagc tgcagggcaa gaagcagtac cagctcaggg gcgttgctac actggagggc
10741 taccgcgagc agaaggctgg ctccagcctc gtcaagcacg ctgaggagat cctgcggaag
10801 cgcggcgcgg atatgatttg gtgcaacgct cggacctcgg cctctggcta ctaccgcaag
10861 ctcggcttct cggagcaggg ggaggtcttc gacacccccc cagttggccc ccatattctg
10921 atgtacaagc ggattactta gctcgatcga gcggccatgc tagagtccgc aaaaatcacc
10981 agtctctctc tacaaatcta tctctctcta tttttctcca gaataatgtg tgagtagttc
11041 ccagataagg gaattagggt tcttataggg tttcgctcat gtgttgagca tataagaaac
11101 ccttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa
11161 ccaaaatcca gtgacctact agtaattcag tacattaaaa acgtccgcaa tgtgttatta
11221 agttgtctaa gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag
11281 ctccccgacc ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagtccgg
11341 gacggcgtca gcgggagagc cgttgtaagg cggcagactt tgctcatgtt accgatgcta
11401 ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag
11461 catgttgatt gtaacgatga cagagcgttg ctgcctgtga tcaccgcggt ttcaaaatcg
11521 gctccgtcga tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct
11581 ggtatttaag gttttagaat gcaaggaaca gtgaattgga gttcgtcttg ttataattag
11641 cttcttgggg tatctttaaa tactgtagaa aagaggaagg aaataataaa tggctaaaat
11701 gagaatatca ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga
11761 aggaatgtct cctgctaagg tatataagct ggtgggagaa aatgaaaacc tatatttaaa
11821 aatgacggac agccggtata aagggaccac ctatgatgtg gaacgggaaa aggacatgat
11881 gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg
11941 ctggagcaat ctgctcatga gtgaggccga tggcgtcctt tgctcggaag agtatgaaga
12001 tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc tctttcactc
12061 catcgacata tcggattgtc cctatacgaa tagcttagac agccgcttag ccgaattgga
12121 ttacttactg aataacgatc tggccgatgt ggattgcgaa aactgggaag aagacactcc
12181 atttaaagat ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg aagaggaact
12241 tgtcttttcc cacggcgacc tgggagacag caacatcttt gtgaaagatg gcaaagtaag
12301 tggctttatt gatcttggga gaagcggcag ggcggacaag tggtatgaca ttgccttctg
12361 cgtccggtcg atcagggagg atatcgggga agaacagtat gtcgagctat tttttgactt
12421 actggggatc aagcctgatt gggagaaaat aaaatattat attttactgg atgaattgtt
12481 ttagtaccta gaatgcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
12541 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
12601 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
12661 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
12721 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
12781 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
12841 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
12901 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
12961 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
13021 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
13081 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
13141 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
13201 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
13261 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
13321 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg
13381 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
13441 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca
13501 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
13561 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
13621 aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg
13681 ccctggtaga ttgcctggcc gtaggccagc catttttgag cggccagcgg ccgcgatagg
13741 ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga agggtaggcg ctttttgcag
13801 ctcttcggct gtgcgctggc cagacagtta tgcacaggcc aggcgggttt taagagtttt
13861 aataagtttt aaagagtttt aggcggaaaa atcgcctttt ttctctttta tatcagtcac
13921 ttacatgtgt gaccggttcc caatgtacgg ctttgggttc ccaatgtacg ggttccggtt
13981 cccaatgtac ggctttgggt tcccaatgta cgtgctatcc acaggaaaca gaccttttcg
14041 acctttttcc cctgctaggg caatttgccc tagcatctgc tccgtacatt aggaaccggc
14101 ggatgcttcg ccctcgatca ggttgcggta gcgcatgact aggatcgggc cagcctgccc
14161 cgcctcctcc ttcaaatcgt actccggcag gtcatttgac ccgatcagct tgcgcacggt
14221 gaaacagaac ttcttgaact ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa
14281 ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat
14341 gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc acgtccgggt tcttgccttc
14401 tgtgatctcg cggtacatcc aatcagctag ctcgatctcg atgtactccg gccgcccggt
14461 ttcgctcttt acgatcttgt agcggctaat caaggcttca ccctcggata ccgtcaccag
14521 gcggccgttc ttggccttct tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat
14581 gcaggtttct accaggtcgt ctttctgctt tccgccatcg gctcgccggc agaacttgag
14641 tacgtccgca acgtgtggac ggaacacgcg gccgggcttg tctcccttcc cttcccggta
14701 tcggttcatg gattcggtta gatgggaaac cgccatcagt accaggtcgt aatcccacac
14761 actggccatg ccggccggcc ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg
14821 gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg aaaacggcca cgtccatgat
14881 gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga acgaaaaaat ctggttgctc
14941 gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct gccggcggtt gccgggattc
15001 tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat
15061 gcgttgccgc tgggcggcct gcgcggcctt caacttctcc accaggtcat cacccagcgc
15121 cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc acgccgattc ctcgggcttg
15181 ggggttccag tgccattgca gggccggcag gcaacccagc cgcttacgcc tggccaaccg
15241 cccgttcctc cacacatggg gcattccacg gcgtcggtgc ctggttgttc ttgattttcc
15301 atgccgcctc ctttagccgc taaaattcat ctactcattt attcatttgc tcatttactc
15361 tggtagctgc gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc
15421 gtacatcttc agcttggtgt gatcctccgc cggcaactga aagttgaccc gcttcatggc
15481 tggcgtgtct gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc
15541 ggcacttagc gtgtttgtgc ttttgctcat tttctcttta cctcattaac tcaaatgagt
15601 tttgatttaa tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct
15661 gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata
15721 cgggactcaa gaatgggcag ctcgtacccg gccagcgcct cggcaacctc accgccgatg
15781 cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt gtagccttcc atccgtgacc
15841 tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg cccatatgtc gtaagggctt
15901 ggctgcaccg gaatcagcac gaagtcggct gccttgatcg cggacacagc caagtccgcc
15961 gcctggggcg ctccgtcgat cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg
16021 tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt gatcttcccg cacggccgcc
16081 caatcgcggg cactgccctg gggatcggaa tcgactaaca gaacatcggc cccggcgagt
16141 tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta
16201 agtacagcga taaccttcat gcgttcccct tgcgtatttg tttatttact catcgcatca
16261 tatacgcagc gaccgcatga cgcaagctgt tttactcaaa tacacatcac ctttttagac
16321 ggcggcgctc ggtttcttca gcggccaagc tggccggcca ggccgccagc ttggcatcag
16381 acaaaccggc caggatttca tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca
16441 cgtacccggc cgcgatcatc tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct
16501 ggccgtcctg gtgcggtttc atgcttgttc ctcttggcgt tcattctcgg cggccgccag
16561 ggcgtcggcc tcggtcaatg cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg
16621 cgtcacttcc tcgctgcgct caagtgcgcg gtacagggtc gagcgatgca cgccaagcag
16681 tgcagccgcc tctttcacgg tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat
16741 ctgtgccggg gtgagggtag ggcgggggcc aaacttcacg cctcgggcct tggcggcctc
16801 gcgcccgctc cgggtgcggt cgatgattag ggaacgctcg aactcggcaa tgccggcgaa
16861 cacggtcaac accatgcggc cggccggcgt ggtggtgtcg gcccacggct ctgccaggct
16921 acgcaggccc gcgccggcct cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct
16981 gcgggccagg cggtctagcc tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag
17041 catcctggcc agctccgggc ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt
17101 ggtgcagccg gccgcgtgca gttcggcccg ttggttggtc aagtcctggt cgtcggtgct
17161 gacgcgggca tagcccagca ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg
17221 gttctagtcg caagtattct actttatgcg actaaaacac gcgacaagaa aacgccagga
17281 aaagggcagg gcggcagcct gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga
17341 agacggctgc actgaacgtc agaagccgac tgcactatag cagcggaggg gttggatcaa
17401 agtactttga tcccgagggg aaccctgtgg ttggcatgca catacaaatg gacgaacgga
17461 taaacctttt cacgcccttt taaatatccg attattctaa taaacgctct tttctcttag
//
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