| 质粒名称: | pMP71-HLA-A0201-His |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 108214 |
| 存储实验室: | Sébastien Walchli |
|
载体骨架基本信息 |
|
| 载体名称: | pMP71 |
| 载体出品公司: | -- |
| 空载体大小: | 5000 bp |
| 完整质粒大小: | 6759 bp |
| 载体修饰: | 转换为网关兼容，请参阅本文：PMID:22132171 |
| 载体类型: | 哺乳细胞表达,逆转录病毒 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄霉素100 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | major histocompatibility complex, class I, B，HLA-A0201 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 1115 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | his（插入件上的C端子） |
| 克隆方法: | -- |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pMP71-HLA-A0201-His was a gift from Sébastien Walchli (Addgene plasmid # 108214 ; http://n2t.net/addgene:108214 ; RRID:Addgene_108214) |
| 参考文献部分: | Breadth and Dynamics of HLA-A2– and HLA-B7–Restricted CD8+ T Cell Responses against Nonstructural Viral Proteins in Acute Human Tick-Borne Encephalitis Virus Infection. Lampen MH, Uchtenhagen H, Blom K, Varnaitė R, Pakalniene J, Dailidyte L, Wälchli S, Lindquist L, Mickiene A, Michaëlsson J, Schumacher TN, Ljunggren H, Sandberg JK, Achour A, Gredmark-Russ S.. ImmunoHorizons (2018) 2 (6), 172-184 10.4049/immunohorizons.1800029 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pMP71-HLA-A0201-His 6758 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION HLA-A0201 his tagged.
ACCESSION .
VERSION .
KEYWORDS pMP71-HLA-A0201-His
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6758)
AUTHORS Lampen MH, Uchtenhagen H, Blom K, Varnaite R, Pakalniene J,
Dailidyte L, Walchli S, Lindquist L, Mickiene A, Michaelsson J,
Schumacher TN, Ljunggren H, Sandberg JK, Achour A, Gredmark-Russ S.
TITLE Breadth and Dynamics of HLA-A2– and HLA-B7–Restricted CD8+ T Cell
Responses against Nonstructural Viral Proteins in Acute Human
Tick-Borne Encephalitis Virus Infection
JOURNAL ImmunoHorizons (2018) 2 (6), 172-184
REFERENCE 2 (bases 1 to 6758)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6758
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 6..25
/label=T7
/note="T7 promoter, forward primer"
promoter 6..24
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 56..80
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 1196..1213
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
protein_bind complement(1239..1263)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
misc_feature 1508..1911
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(3032..3048)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3032..3048)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(3045..3067)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 3056..3072
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3080..3110)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3125..3146
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(3263..3280)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3434..4022)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4193..5053)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4816..4835
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5054..5158)
/gene="bla"
/label=AmpR promoter
primer_bind 5226..5244
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(5282..5304)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 5404..5423
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 5617..5639
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 5631..5648
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 5632..5648
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 tcgagtaata cgactcacta tagggagacc caagctggct aggtaagctt gatcaacaag
61 tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc atggccgtca tggcgccccg
121 aaccctcgtc ctgctactct cgggggctct ggccctgacc cagacctggg cgggctctca
181 ctccatgagg tatttcttca catccgtgtc ccggcccggc cgcggggagc cccgcttcat
241 cgcagtgggc tacgtggacg acacgcagtt cgtgcggttc gacagcgacg ccgcgagcca
301 gaggatggag ccgcgggcgc cgtggataga gcaggagggt ccggagtatt gggacgggga
361 gacacggaaa gtgaaggccc actcacagac tcaccgagtg gacctgggga ccctgcgcgg
421 ctactacaac cagagcgagg ccggttctca caccgtccag aggatgtatg gctgcgacgt
481 ggggtcggac tggcgcttcc tccgcgggta ccaccagtac gcctacgacg gcaaggatta
541 catcgccctg aaagaggacc tgcgctcttg gaccgcggcg gacatggcag ctcagaccac
601 caagcacaag tgggaggcgg cccatgtggc ggagcagttg agagcctacc tggagggcac
661 gtgcgtggag tggctccgca gatacctgga gaacgggaag gagacgctgc agcgcacgga
721 cgcccccaaa acgcatatga ctcaccacgc tgtctctgac catgaagcca ccctgaggtg
781 ctgggccctg agcttctacc ctgcggagat cacactgacc tggcagcggg atggggagga
841 ccagacccag gacacggagc tcgtggagac caggcctgca ggggatggaa ccttccagaa
901 gtgggcggct gtggtggtgc cttctggaca ggagcagaga tacacctgcc atgtgcagca
961 tgagggtttg cccaagcccc tcaccctgag atgggagccg tcttcccagc ccaccatccc
1021 catcgtgggc atcattgctg gcctggttct ctttggagct gtgatcactg gagctgtggt
1081 cgctgctgtg atgtggagga ggaagagctc agatagaaaa ggagggagct actctcaggc
1141 tgcaagcagt gacagtgccc agggctctga tgtgtctctc acagcttgta aagtgcatca
1201 ccatcaccat cactgactcg agaagggtgg gcgcgccgac ccagctttct tgtacaaagt
1261 ggttgatcta gagggcccgc ggttcgaagg taagcctatc cctaaccctc aattcgagca
1321 tcttaccgcc atttattccc atatttgttc tgtttttctt gatttgggta tacatttaaa
1381 tgttaataaa acaaaatggt ggggcaatca tttacatttt atgggatatg taattactag
1441 ttcaggtgta ttgccacaag acaaacatgt taagaaactt tcccgttatt tacgctctgt
1501 tcctgttaat caacctctgg attacaaaat ttgtgaaaga ttgactgata ttcttaacta
1561 tgttgctcct tttacgctgt gtggatatgc tgctttaatg cctctgtatc atgctattgc
1621 ttcccgtacg gctttcgttt tctcctcctt gtataaatcc tggttgctgt ctctttatga
1681 ggagttgtgg cccgttgtcc gtcaacgtgg cgtggtgtgc tctgtgtttg ctgacgcaac
1741 ccccactggc tggggcattg ccaccacctg tcaactcctt tctgggactt tcgctttccc
1801 cctcccgatc gccacggcag aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
1861 taggttgctg ggcactgata attccgtggt gttgtcgggg aagctgacgt cctttccatg
1921 gctgctcgcc tgtgttgcca actggatcct gcgcgggacg tccttctgct acgtcccttc
1981 ggctctcaat ccagcggacc tcccttcccg aggccttctg ccggttctgc ggcctctccc
2041 gcgtcttcgc tttcggcctc cgacgagtcg gatctccctt tgggccgcct ccccgcctgt
2101 ttcgcctcgg cgtccggtcc gtgttgcttg gtcgtcacct gtgcagaatt gcgaaccatg
2161 gattccaccg tgaactttgt ctcctggcat gcaaatcgtc aacttggcat gccaagaata
2221 attcggatcc aagcttaggc ctgctcgctt tcttgctgtc ccatttctat taaaggttcc
2281 tttgttccct aagtccaact actaaactgg gggatattat gaagggcctt gagcatctgg
2341 attctgccta gcgctaagct taacacgagc catagataga ataaaagatt ttatttagtc
2401 tccagaaaaa ggggggaatg aaagacccca cctgtaggtt tggcaagcta gcttaagtaa
2461 cgccattttg caaggcatgg aaaatacata actgagaata gagaagttca gatcaaggtt
2521 aggaacagag agacagcaga atatgggcca aacaggatat ctgtggtaag cagttcctgc
2581 cccggctcag ggccaagaac agttggaaca gcagaatatg ggccaaacag gatatctgtg
2641 gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtcccgc
2701 cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga
2761 ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct
2821 gctccccgag ctcaataaaa gagcccacaa cccctcactc ggcgcgccag tcctccgata
2881 gactgcgtcg cccgggtacc cgtgttctca ataaaccctc ttgcagttgc atccgactcg
2941 tggtctcgct gttccttggg agggtctcct ctgagtgatt gactgcccac ctcgggggtc
3001 tttcattctc gagcagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
3061 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
3121 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
3181 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
3241 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
3301 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
3361 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
3421 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
3481 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt cgccctggaa
3541 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
3601 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
3661 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
3721 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
3781 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
3841 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
3901 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
3961 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
4021 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
4081 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
4141 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
4201 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
4261 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
4321 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
4381 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
4441 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
4501 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
4561 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
4621 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
4681 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
4741 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
4801 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
4861 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
4921 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
4981 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
5041 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
5101 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
5161 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
5221 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
5281 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
5341 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
5401 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
5461 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt
5521 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
5581 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
5641 cggccagtga attagtactc tagcttaagt aacgccattt tgcaaggcat ggaaaataca
5701 taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca gaatatgggc
5761 caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga acagttggaa
5821 cagcagaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc
5881 caagaacaga tggtccccag atgcggtccc gccctcagca gtttctagag aaccatcaga
5941 tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc
6001 agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa aagagcccac
6061 aacccctcac tcggcgcgcc agtcctccga tagactgcgt cgcccgggta cccgtattcc
6121 caataaagcc tcttgctgtt tgcatccgaa tcgtggactc gctgatcctt gggagggtct
6181 cctcagattg attgactgcc cacctcgggg gtctttcatt tggaggttcc accgagattt
6241 ggagacccct gcccagggac caccgacccc cccgccggga ggtaagctgg ccagcggtcg
6301 tttcgtgtct gtctctgtct ttgtgcgtgt ttgtgccggc atctaatgtt tgcgcctgcg
6361 tctgtactag ttggctaact agatctgtat ctggcggtcc cgcggaagaa ctgacgagtt
6421 cgtattcccg gccgcagccc ctgggagacg tcccagcggc ctcgggggcc cgttttgtgg
6481 cccattctgt atcagttaac ctacccgagt cggacttttt ggagctccgc cactgtccga
6541 ggggtacgtg gctttgttgg gggacgagag acagagacac ttcccgcccc cgtctgaatt
6601 tttgctttcg gttttacgcc gaaaccgcgc cgcgcgtctt gtctgctgca gcatcgttct
6661 gtgttgtctc tgtctgactg tgtttctgta tttgtctgaa aattagctcg acaaagttaa
6721 gtaatagtcc ctctctccaa gctcacttac aggcggcc
//
