PX388

价格:1000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Addgene
载体名称: PX388 或 PX388 Bacteroides coprophilus Cas9
质粒类型: CRISPR-Cas9载体;基因敲除载体;昆虫细胞载体
高拷贝/低拷贝: 高拷贝
插入位点: 限制性内切酶,多克隆位点
启动子: CMV
载体大小: 11723 bp
5' 测序引物及序列: CMV Forward:CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列: --
载体标签: NLS (C ter);HA、NLS (N ter)
载体抗性: 氨苄青霉素
筛选标记: Neomycin
克隆菌株: Stbl3
宿主细胞(系): 昆虫细胞
备注: --
产品目录号: #68325
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 杆状病毒

载体质粒图谱和多克隆位点信息

PX388载体图谱



载体简介

载体序列

LOCUS       PX388 Bacteroides coprophilus Cas9	11723 bp 	DNA	SYN
DEFINITION  PX388 Bacteroides coprophilus Cas9
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..11723
                     /organism="PX388 Bacteroides coprophilus Cas9"
                     /mol_type="other DNA"
     misc_feature    47..1001
                     /label="Ac_5_flank"
     CDS             complement(343..948)
                     /label="ORF frame 3"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     promoter        1023..1587
                     /label="CMV_immearly_promoter"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1090..1377
                     /label="CAG_enhancer"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1544..1564
                     /label="CMV_fwd_primer"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     promoter        1615..1633
                     /label="T7_promoter"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1633..1656
                     /label="lacO"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1772..1796
                     /label="attB1"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     CDS             1822..6414
                     /label="ORF frame 1"
                     /translation="MYPYDVPDYASPKKKRKVEASMKKTLGLDLGTNSIGWAVINSNI
                     DSEGKEKLVGISSCGSRIIPMDATTLGDFGKGNTKSPVAERTRLRGIRRLLERSLLRR
                     ERLHRVLSVMGFLPEHYASQLDRYGKFLPETEPKLAWYKDDSGRYQFLFQKSFHEMLE
                     DFRQHQPELVAGEKKIPYDWTIYYLRKKALSQEITKEELAWILLNFNQKRGYYQLRGE
                     EEQEENNKSVEYHALKVVSVEDSGERKGKDIWYNVTLENGWVYRRASNIPLDWTGKVK
                     EFVVTTELDDAGNPKKDKEGNVKRSFRAPKEDDWGLLKTRTQAQIDESGKTVGTYIYE
                     SLLCMPNQKIRGKLVRTIERKYYKDELRQILVKQSEFHAALQDHNLLLSCIEELYPNN
                     EAHRRLLSASSFIYFLIEDILFYQRPLKSQKGLIDNCPYESHIYKDKKDGSLHHVPLK
                     CVSKSHPLFQEFRLWQFLSNLRIYQRERMMDGSLKLDVDVTREFLPSEEDYVKLFDWL
                     NERKEISQKFLLAYKPFGLKKNEQANYRWNYVEDKSYPCNETRAEIKSRLSKAGVPEE
                     FLTEEKEEALWHILYSISDKKELTKALGTFAAKNCLNESFVEVFAKIPPFESNYAAYS
                     LKAIRKLLALMRMGKYWNEQAIDRQTRDRIEKILTGEYDETIRSRVREKAMLLTDISS
                     FRGLPLWLACYIVYDRHSESKELVKWETPADIDHFLSKFKQNSLRNPIVEQVITESLR
                     TVRDIWKQEGKIDEIHVELGREMKNPAKERARITAQVQENENTNLRIKALLAEFMNPE
                     FEIENVHPYSPGQQEILRIYEDGVLSGIAEKDLPDDITAILKKFRENDVKKRPTTSEV
                     LRYKLWLEQRYRSPYTGRVIPLGKLFTPAYEIEHVIPQSRYFDDSISNKVICESAVNK
                     LKDNCLGYEFIKKHSGEMVELGNGETVPVFSVEEYERFVKESYFGNSKKMKKLLLEDI
                     PDSFIERQLNDSRYISRVVTSLLSNLVCEEGEQDGLSKNVIVCTGGITDRLKKDWGVQ
                     EVWNRIILPRFLRLNEITGRTDFTSTSVNGHLLPALPLYLQKGFNKKRIDHRHHAMDA
                     IVIACANRNIVNYLNNSSARKNSEISRYDLQRLLCEKVKTDANGNYKWILRKPWETFT
                     QDVYAALTNIVVSFKQNLRVINRTTNYYQHYNEAGEKRMIPQTKGDRWAIRKPMHKDT
                     VYGEVNLRKEKTLPLRDVVKNPSIVVDKSLKNKLYELLKSQYDLKAIAKYFETHQDVW
                     ADVNLKKIKVYYFTKETNERFFATRKSLDPSFDQKKIEEEVTDTGVQKILLHHLQQNN
                     NDPDMAFSPDGIDRMNQNMTILNDGKWHQPIYKVRTYEKADKFAVGESGNKAKKFVEA
                     AKGTNLFFAVYESVQEDEAIGKQVCKRTFATIPLNEVIKRKKQGLPAAPEDLNGNLPK
                     FVLSPNDLVYLPTEEERNSSRIIQPLDRERIYKMVSSSGSQCFFIKVFVANSIWDKNE
                     YSSLNKMERAITNEMIKEICVPIKIDRLGNVSLIQI*"
     misc_feature    1825..1851
                     /label="HA"
                     /translation="MYPYDVPDYASPKKKRKVEASMKKTLGLDLGTNSIGWAVINSNI
                     DSEGKEKLVGISSCGSRIIPMDATTLGDFGKGNTKSPVAERTRLRGIRRLLERSLLRR
                     ERLHRVLSVMGFLPEHYASQLDRYGKFLPETEPKLAWYKDDSGRYQFLFQKSFHEMLE
                     DFRQHQPELVAGEKKIPYDWTIYYLRKKALSQEITKEELAWILLNFNQKRGYYQLRGE
                     EEQEENNKSVEYHALKVVSVEDSGERKGKDIWYNVTLENGWVYRRASNIPLDWTGKVK
                     EFVVTTELDDAGNPKKDKEGNVKRSFRAPKEDDWGLLKTRTQAQIDESGKTVGTYIYE
                     SLLCMPNQKIRGKLVRTIERKYYKDELRQILVKQSEFHAALQDHNLLLSCIEELYPNN
                     EAHRRLLSASSFIYFLIEDILFYQRPLKSQKGLIDNCPYESHIYKDKKDGSLHHVPLK
                     CVSKSHPLFQEFRLWQFLSNLRIYQRERMMDGSLKLDVDVTREFLPSEEDYVKLFDWL
                     NERKEISQKFLLAYKPFGLKKNEQANYRWNYVEDKSYPCNETRAEIKSRLSKAGVPEE
                     FLTEEKEEALWHILYSISDKKELTKALGTFAAKNCLNESFVEVFAKIPPFESNYAAYS
                     LKAIRKLLALMRMGKYWNEQAIDRQTRDRIEKILTGEYDETIRSRVREKAMLLTDISS
                     FRGLPLWLACYIVYDRHSESKELVKWETPADIDHFLSKFKQNSLRNPIVEQVITESLR
                     TVRDIWKQEGKIDEIHVELGREMKNPAKERARITAQVQENENTNLRIKALLAEFMNPE
                     FEIENVHPYSPGQQEILRIYEDGVLSGIAEKDLPDDITAILKKFRENDVKKRPTTSEV
                     LRYKLWLEQRYRSPYTGRVIPLGKLFTPAYEIEHVIPQSRYFDDSISNKVICESAVNK
                     LKDNCLGYEFIKKHSGEMVELGNGETVPVFSVEEYERFVKESYFGNSKKMKKLLLEDI
                     PDSFIERQLNDSRYISRVVTSLLSNLVCEEGEQDGLSKNVIVCTGGITDRLKKDWGVQ
                     EVWNRIILPRFLRLNEITGRTDFTSTSVNGHLLPALPLYLQKGFNKKRIDHRHHAMDA
                     IVIACANRNIVNYLNNSSARKNSEISRYDLQRLLCEKVKTDANGNYKWILRKPWETFT
                     QDVYAALTNIVVSFKQNLRVINRTTNYYQHYNEAGEKRMIPQTKGDRWAIRKPMHKDT
                     VYGEVNLRKEKTLPLRDVVKNPSIVVDKSLKNKLYELLKSQYDLKAIAKYFETHQDVW
                     ADVNLKKIKVYYFTKETNERFFATRKSLDPSFDQKKIEEEVTDTGVQKILLHHLQQNN
                     NDPDMAFSPDGIDRMNQNMTILNDGKWHQPIYKVRTYEKADKFAVGESGNKAKKFVEA
                     AKGTNLFFAVYESVQEDEAIGKQVCKRTFATIPLNEVIKRKKQGLPAAPEDLNGNLPK
                     FVLSPNDLVYLPTEEERNSSRIIQPLDRERIYKMVSSSGSQCFFIKVFVANSIWDKNE
                     YSSLNKMERAITNEMIKEICVPIKIDRLGNVSLIQI*"
     misc_feature    1855..1875
                     /label="SV40 NLS"
                     /translation="MYPYDVPDYASPKKKRKVEASMKKTLGLDLGTNSIGWAVINSNI
                     DSEGKEKLVGISSCGSRIIPMDATTLGDFGKGNTKSPVAERTRLRGIRRLLERSLLRR
                     ERLHRVLSVMGFLPEHYASQLDRYGKFLPETEPKLAWYKDDSGRYQFLFQKSFHEMLE
                     DFRQHQPELVAGEKKIPYDWTIYYLRKKALSQEITKEELAWILLNFNQKRGYYQLRGE
                     EEQEENNKSVEYHALKVVSVEDSGERKGKDIWYNVTLENGWVYRRASNIPLDWTGKVK
                     EFVVTTELDDAGNPKKDKEGNVKRSFRAPKEDDWGLLKTRTQAQIDESGKTVGTYIYE
                     SLLCMPNQKIRGKLVRTIERKYYKDELRQILVKQSEFHAALQDHNLLLSCIEELYPNN
                     EAHRRLLSASSFIYFLIEDILFYQRPLKSQKGLIDNCPYESHIYKDKKDGSLHHVPLK
                     CVSKSHPLFQEFRLWQFLSNLRIYQRERMMDGSLKLDVDVTREFLPSEEDYVKLFDWL
                     NERKEISQKFLLAYKPFGLKKNEQANYRWNYVEDKSYPCNETRAEIKSRLSKAGVPEE
                     FLTEEKEEALWHILYSISDKKELTKALGTFAAKNCLNESFVEVFAKIPPFESNYAAYS
                     LKAIRKLLALMRMGKYWNEQAIDRQTRDRIEKILTGEYDETIRSRVREKAMLLTDISS
                     FRGLPLWLACYIVYDRHSESKELVKWETPADIDHFLSKFKQNSLRNPIVEQVITESLR
                     TVRDIWKQEGKIDEIHVELGREMKNPAKERARITAQVQENENTNLRIKALLAEFMNPE
                     FEIENVHPYSPGQQEILRIYEDGVLSGIAEKDLPDDITAILKKFRENDVKKRPTTSEV
                     LRYKLWLEQRYRSPYTGRVIPLGKLFTPAYEIEHVIPQSRYFDDSISNKVICESAVNK
                     LKDNCLGYEFIKKHSGEMVELGNGETVPVFSVEEYERFVKESYFGNSKKMKKLLLEDI
                     PDSFIERQLNDSRYISRVVTSLLSNLVCEEGEQDGLSKNVIVCTGGITDRLKKDWGVQ
                     EVWNRIILPRFLRLNEITGRTDFTSTSVNGHLLPALPLYLQKGFNKKRIDHRHHAMDA
                     IVIACANRNIVNYLNNSSARKNSEISRYDLQRLLCEKVKTDANGNYKWILRKPWETFT
                     QDVYAALTNIVVSFKQNLRVINRTTNYYQHYNEAGEKRMIPQTKGDRWAIRKPMHKDT
                     VYGEVNLRKEKTLPLRDVVKNPSIVVDKSLKNKLYELLKSQYDLKAIAKYFETHQDVW
                     ADVNLKKIKVYYFTKETNERFFATRKSLDPSFDQKKIEEEVTDTGVQKILLHHLQQNN
                     NDPDMAFSPDGIDRMNQNMTILNDGKWHQPIYKVRTYEKADKFAVGESGNKAKKFVEA
                     AKGTNLFFAVYESVQEDEAIGKQVCKRTFATIPLNEVIKRKKQGLPAAPEDLNGNLPK
                     FVLSPNDLVYLPTEEERNSSRIIQPLDRERIYKMVSSSGSQCFFIKVFVANSIWDKNE
                     YSSLNKMERAITNEMIKEICVPIKIDRLGNVSLIQI*"
     misc_feature    1861..1875
                     /label="NLS"
                     /translation="MYPYDVPDYASPKKKRKVEASMKKTLGLDLGTNSIGWAVINSNI
                     DSEGKEKLVGISSCGSRIIPMDATTLGDFGKGNTKSPVAERTRLRGIRRLLERSLLRR
                     ERLHRVLSVMGFLPEHYASQLDRYGKFLPETEPKLAWYKDDSGRYQFLFQKSFHEMLE
                     DFRQHQPELVAGEKKIPYDWTIYYLRKKALSQEITKEELAWILLNFNQKRGYYQLRGE
                     EEQEENNKSVEYHALKVVSVEDSGERKGKDIWYNVTLENGWVYRRASNIPLDWTGKVK
                     EFVVTTELDDAGNPKKDKEGNVKRSFRAPKEDDWGLLKTRTQAQIDESGKTVGTYIYE
                     SLLCMPNQKIRGKLVRTIERKYYKDELRQILVKQSEFHAALQDHNLLLSCIEELYPNN
                     EAHRRLLSASSFIYFLIEDILFYQRPLKSQKGLIDNCPYESHIYKDKKDGSLHHVPLK
                     CVSKSHPLFQEFRLWQFLSNLRIYQRERMMDGSLKLDVDVTREFLPSEEDYVKLFDWL
                     NERKEISQKFLLAYKPFGLKKNEQANYRWNYVEDKSYPCNETRAEIKSRLSKAGVPEE
                     FLTEEKEEALWHILYSISDKKELTKALGTFAAKNCLNESFVEVFAKIPPFESNYAAYS
                     LKAIRKLLALMRMGKYWNEQAIDRQTRDRIEKILTGEYDETIRSRVREKAMLLTDISS
                     FRGLPLWLACYIVYDRHSESKELVKWETPADIDHFLSKFKQNSLRNPIVEQVITESLR
                     TVRDIWKQEGKIDEIHVELGREMKNPAKERARITAQVQENENTNLRIKALLAEFMNPE
                     FEIENVHPYSPGQQEILRIYEDGVLSGIAEKDLPDDITAILKKFRENDVKKRPTTSEV
                     LRYKLWLEQRYRSPYTGRVIPLGKLFTPAYEIEHVIPQSRYFDDSISNKVICESAVNK
                     LKDNCLGYEFIKKHSGEMVELGNGETVPVFSVEEYERFVKESYFGNSKKMKKLLLEDI
                     PDSFIERQLNDSRYISRVVTSLLSNLVCEEGEQDGLSKNVIVCTGGITDRLKKDWGVQ
                     EVWNRIILPRFLRLNEITGRTDFTSTSVNGHLLPALPLYLQKGFNKKRIDHRHHAMDA
                     IVIACANRNIVNYLNNSSARKNSEISRYDLQRLLCEKVKTDANGNYKWILRKPWETFT
                     QDVYAALTNIVVSFKQNLRVINRTTNYYQHYNEAGEKRMIPQTKGDRWAIRKPMHKDT
                     VYGEVNLRKEKTLPLRDVVKNPSIVVDKSLKNKLYELLKSQYDLKAIAKYFETHQDVW
                     ADVNLKKIKVYYFTKETNERFFATRKSLDPSFDQKKIEEEVTDTGVQKILLHHLQQNN
                     NDPDMAFSPDGIDRMNQNMTILNDGKWHQPIYKVRTYEKADKFAVGESGNKAKKFVEA
                     AKGTNLFFAVYESVQEDEAIGKQVCKRTFATIPLNEVIKRKKQGLPAAPEDLNGNLPK
                     FVLSPNDLVYLPTEEERNSSRIIQPLDRERIYKMVSSSGSQCFFIKVFVANSIWDKNE
                     YSSLNKMERAITNEMIKEICVPIKIDRLGNVSLIQI*"
     CDS             complement(2149..3189)
                     /label="ORF frame 3"
                     /translation="MGFADTLQRHMVQTSIFLILVDVRLVGAVIDQPFLGLQWALIKQ
                     NVLDQEVDEAAGAQQPSVGLIVRVQLFDTGQQQIMILQSGVELALLHQDLSQLVFIVL
                     AFNGPHQFSPDLLVGHTQQRLVDVSSHGLAGFVDLSLGPSFQQPPIVLLWCPKGSFHI
                     SFLIFLWVPGIVQLGSDHELFHLAGPVQGNVASPSIDPSVLQSHIVPDIFALSFSRVL
                     DAHHFQGVILHALIVLFLLFLPSQLIVAAFLVEIQQNPGQFLLSDLLRQCFLPQIVDC
                     PIVRDFLLSCHQFWLMLSKVLQHLVEGLLKQELIPAAIVFVPCQLGLGFWQKLSIAVQ
                     LASVMLWQEPHD*"
     misc_feature    complement(6447..6464)
                     /label="Sp6_primer"
                     /translation="MGFADTLQRHMVQTSIFLILVDVRLVGAVIDQPFLGLQWALIKQ
                     NVLDQEVDEAAGAQQPSVGLIVRVQLFDTGQQQIMILQSGVELALLHQDLSQLVFIVL
                     AFNGPHQFSPDLLVGHTQQRLVDVSSHGLAGFVDLSLGPSFQQPPIVLLWCPKGSFHI
                     SFLIFLWVPGIVQLGSDHELFHLAGPVQGNVASPSIDPSVLQSHIVPDIFALSFSRVL
                     DAHHFQGVILHALIVLFLLFLPSQLIVAAFLVEIQQNPGQFLLSDLLRQCFLPQIVDC
                     PIVRDFLLSCHQFWLMLSKVLQHLVEGLLKQELIPAAIVFVPCQLGLGFWQKLSIAVQ
                     LASVMLWQEPHD*"
     misc_feature    6469..6493
                     /label="attB2"
                     /translation="MGFADTLQRHMVQTSIFLILVDVRLVGAVIDQPFLGLQWALIKQ
                     NVLDQEVDEAAGAQQPSVGLIVRVQLFDTGQQQIMILQSGVELALLHQDLSQLVFIVL
                     AFNGPHQFSPDLLVGHTQQRLVDVSSHGLAGFVDLSLGPSFQQPPIVLLWCPKGSFHI
                     SFLIFLWVPGIVQLGSDHELFHLAGPVQGNVASPSIDPSVLQSHIVPDIFALSFSRVL
                     DAHHFQGVILHALIVLFLLFLPSQLIVAAFLVEIQQNPGQFLLSDLLRQCFLPQIVDC
                     PIVRDFLLSCHQFWLMLSKVLQHLVEGLLKQELIPAAIVFVPCQLGLGFWQKLSIAVQ
                     LASVMLWQEPHD*"
     terminator      6505..6971
                     /label="hGH_PA_terminator"
                     /translation="MGFADTLQRHMVQTSIFLILVDVRLVGAVIDQPFLGLQWALIKQ
                     NVLDQEVDEAAGAQQPSVGLIVRVQLFDTGQQQIMILQSGVELALLHQDLSQLVFIVL
                     AFNGPHQFSPDLLVGHTQQRLVDVSSHGLAGFVDLSLGPSFQQPPIVLLWCPKGSFHI
                     SFLIFLWVPGIVQLGSDHELFHLAGPVQGNVASPSIDPSVLQSHIVPDIFALSFSRVL
                     DAHHFQGVILHALIVLFLLFLPSQLIVAAFLVEIQQNPGQFLLSDLLRQCFLPQIVDC
                     PIVRDFLLSCHQFWLMLSKVLQHLVEGLLKQELIPAAIVFVPCQLGLGFWQKLSIAVQ
                     LASVMLWQEPHD*"
     terminator      6977..7012
                     /label="T7_terminator"
                     /translation="MGFADTLQRHMVQTSIFLILVDVRLVGAVIDQPFLGLQWALIKQ
                     NVLDQEVDEAAGAQQPSVGLIVRVQLFDTGQQQIMILQSGVELALLHQDLSQLVFIVL
                     AFNGPHQFSPDLLVGHTQQRLVDVSSHGLAGFVDLSLGPSFQQPPIVLLWCPKGSFHI
                     SFLIFLWVPGIVQLGSDHELFHLAGPVQGNVASPSIDPSVLQSHIVPDIFALSFSRVL
                     DAHHFQGVILHALIVLFLLFLPSQLIVAAFLVEIQQNPGQFLLSDLLRQCFLPQIVDC
                     PIVRDFLLSCHQFWLMLSKVLQHLVEGLLKQELIPAAIVFVPCQLGLGFWQKLSIAVQ
                     LASVMLWQEPHD*"
     rep_origin      7129..7435
                     /label="f1_origin"
                     /translation="MGFADTLQRHMVQTSIFLILVDVRLVGAVIDQPFLGLQWALIKQ
                     NVLDQEVDEAAGAQQPSVGLIVRVQLFDTGQQQIMILQSGVELALLHQDLSQLVFIVL
                     AFNGPHQFSPDLLVGHTQQRLVDVSSHGLAGFVDLSLGPSFQQPPIVLLWCPKGSFHI
                     SFLIFLWVPGIVQLGSDHELFHLAGPVQGNVASPSIDPSVLQSHIVPDIFALSFSRVL
                     DAHHFQGVILHALIVLFLLFLPSQLIVAAFLVEIQQNPGQFLLSDLLRQCFLPQIVDC
                     PIVRDFLLSCHQFWLMLSKVLQHLVEGLLKQELIPAAIVFVPCQLGLGFWQKLSIAVQ
                     LASVMLWQEPHD*"
     misc_feature    complement(7549..7569)
                     /label="pBABE_3_primer"
                     /translation="MGFADTLQRHMVQTSIFLILVDVRLVGAVIDQPFLGLQWALIKQ
                     NVLDQEVDEAAGAQQPSVGLIVRVQLFDTGQQQIMILQSGVELALLHQDLSQLVFIVL
                     AFNGPHQFSPDLLVGHTQQRLVDVSSHGLAGFVDLSLGPSFQQPPIVLLWCPKGSFHI
                     SFLIFLWVPGIVQLGSDHELFHLAGPVQGNVASPSIDPSVLQSHIVPDIFALSFSRVL
                     DAHHFQGVILHALIVLFLLFLPSQLIVAAFLVEIQQNPGQFLLSDLLRQCFLPQIVDC
                     PIVRDFLLSCHQFWLMLSKVLQHLVEGLLKQELIPAAIVFVPCQLGLGFWQKLSIAVQ
                     LASVMLWQEPHD*"
     misc_feature    complement(7555..7770)
                     /label="SV40_enhancer"
                     /translation="MGFADTLQRHMVQTSIFLILVDVRLVGAVIDQPFLGLQWALIKQ
                     NVLDQEVDEAAGAQQPSVGLIVRVQLFDTGQQQIMILQSGVELALLHQDLSQLVFIVL
                     AFNGPHQFSPDLLVGHTQQRLVDVSSHGLAGFVDLSLGPSFQQPPIVLLWCPKGSFHI
                     SFLIFLWVPGIVQLGSDHELFHLAGPVQGNVASPSIDPSVLQSHIVPDIFALSFSRVL
                     DAHHFQGVILHALIVLFLLFLPSQLIVAAFLVEIQQNPGQFLLSDLLRQCFLPQIVDC
                     PIVRDFLLSCHQFWLMLSKVLQHLVEGLLKQELIPAAIVFVPCQLGLGFWQKLSIAVQ
                     LASVMLWQEPHD*"
     promoter        7567..7835
                     /label="SV40_promoter"
                     /translation="MGFADTLQRHMVQTSIFLILVDVRLVGAVIDQPFLGLQWALIKQ
                     NVLDQEVDEAAGAQQPSVGLIVRVQLFDTGQQQIMILQSGVELALLHQDLSQLVFIVL
                     AFNGPHQFSPDLLVGHTQQRLVDVSSHGLAGFVDLSLGPSFQQPPIVLLWCPKGSFHI
                     SFLIFLWVPGIVQLGSDHELFHLAGPVQGNVASPSIDPSVLQSHIVPDIFALSFSRVL
                     DAHHFQGVILHALIVLFLLFLPSQLIVAAFLVEIQQNPGQFLLSDLLRQCFLPQIVDC
                     PIVRDFLLSCHQFWLMLSKVLQHLVEGLLKQELIPAAIVFVPCQLGLGFWQKLSIAVQ
                     LASVMLWQEPHD*"
     rep_origin      7734..7811
                     /label="SV40_origin"
                     /translation="MGFADTLQRHMVQTSIFLILVDVRLVGAVIDQPFLGLQWALIKQ
                     NVLDQEVDEAAGAQQPSVGLIVRVQLFDTGQQQIMILQSGVELALLHQDLSQLVFIVL
                     AFNGPHQFSPDLLVGHTQQRLVDVSSHGLAGFVDLSLGPSFQQPPIVLLWCPKGSFHI
                     SFLIFLWVPGIVQLGSDHELFHLAGPVQGNVASPSIDPSVLQSHIVPDIFALSFSRVL
                     DAHHFQGVILHALIVLFLLFLPSQLIVAAFLVEIQQNPGQFLLSDLLRQCFLPQIVDC
                     PIVRDFLLSCHQFWLMLSKVLQHLVEGLLKQELIPAAIVFVPCQLGLGFWQKLSIAVQ
                     LASVMLWQEPHD*"
     misc_feature    7796..7815
                     /label="SV40pro_F_primer"
                     /translation="MGFADTLQRHMVQTSIFLILVDVRLVGAVIDQPFLGLQWALIKQ
                     NVLDQEVDEAAGAQQPSVGLIVRVQLFDTGQQQIMILQSGVELALLHQDLSQLVFIVL
                     AFNGPHQFSPDLLVGHTQQRLVDVSSHGLAGFVDLSLGPSFQQPPIVLLWCPKGSFHI
                     SFLIFLWVPGIVQLGSDHELFHLAGPVQGNVASPSIDPSVLQSHIVPDIFALSFSRVL
                     DAHHFQGVILHALIVLFLLFLPSQLIVAAFLVEIQQNPGQFLLSDLLRQCFLPQIVDC
                     PIVRDFLLSCHQFWLMLSKVLQHLVEGLLKQELIPAAIVFVPCQLGLGFWQKLSIAVQ
                     LASVMLWQEPHD*"
     CDS             7950..8744
                     /label="ORF frame 3"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     gene            7953..8741
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     CDS             complement(8259..8795)
                     /label="ORF frame 1"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     terminator      8921..9040
                     /label="SV40_PA_terminator"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    9009..9028
                     /label="EBV_rev_primer"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     terminator      9231..9266
                     /label="T7_terminator"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    complement(9289..9306)
                     /label="Baculovirs_rev_primer"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    9296..9373
                     /label="3_AcPH"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     CDS             complement(9403..10020)
                     /label="ORF frame 3"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     misc_feature    10000..10033
                     /label="loxP"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     gene            complement(10801..11661)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     CDS             complement(10801..11661)
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
   61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
  121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
  181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
  241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
  301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
  361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
  421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
  481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
  541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
  601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
  661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
  721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
  781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
  841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
  901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
  961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc
 1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
 1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
 1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
 1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
 1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
 1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
 1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
 1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
 1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
 1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac
 1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac
 1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac
 1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg
 1801 agatataccA CCGGTGCCAC CATGTACCCA TACGATGTTC CAGATTACGC TTCGCCGAAG
 1861 AAAAAGCGCA AGGTCGAAGC GTCCATGAAG AAAACACTGG GACTGGACCT GGGGACTAAC
 1921 AGCATTGGGT GGGCCGTCAT CAACTCAAAC ATCGACTCAG AAGGAAAAGA AAAGCTGGTG
 1981 GGGATCAGCT CCTGCGGAAG CCGGATCATT CCTATGGACG CTACCACACT GGGAGATTTC
 2041 GGAAAGGGCA ACACTAAAAG TCCAGTGGCA GAGCGAACCC GACTGCGGGG CATTCGGAGA
 2101 CTGCTGGAAA GGTCACTGCT GAGGCGCGAG CGACTGCACC GAGTGCTGTC AGTCATGGGG
 2161 TTCCTGCCAG AGCATTACGC TAGCCAGCTG GACCGCTATG GAAAGTTTCT GCCAGAAACC
 2221 GAGCCCAAGC TGGCATGGTA CAAAGACGAT AGCGGCAGGT ATCAGTTCCT GTTTCAGAAG
 2281 TCCTTCCACG AGATGCTGGA GGACTTTCGA CAGCATCAGC CAGAACTGGT GGCAGGAGAG
 2341 AAGAAAATCC CTTACGATTG GACAATCTAC TATCTGCGGA AGAAAGCACT GTCTCAGGAG
 2401 ATCACTAAGG AGGAACTGGC CTGGATTCTG CTGAATTTCA ACCAGAAACG CGGCTACTAT
 2461 CAGCTGCGAG GGGAGGAAGA GCAGGAAGAG AACAATAAGA GCGTGGAGTA TCACGCCCTG
 2521 AAAGTGGTGA GCGTCGAGGA CTCTGGAGAA AGAAAGGGCA AAGATATCTG GTACAATGTG
 2581 ACTCTGGAGA ACGGATGGGT CTATCGACGG GCTAGCAACA TTCCCCTGGA CTGGACCGGC
 2641 AAGGTGAAAG AGTTCGTGGT CACTACCGAG CTGGACGATG CCGGGAACCC AAAGAAAGAT
 2701 AAGGAAGGAA ATGTGAAAAG ATCCTTTAGG GCACCAAAGG AGGACGATTG GGGGCTGCTG
 2761 AAAACTAGGA CCCAGGCTCA GATCGACGAA TCCGGCAAGA CCGTGGGAAC TTACATCTAC
 2821 GAGTCTCTGC TGTGTATGCC CAACCAGAAG ATCCGGGGAA AACTGGTGAG GACCATTGAA
 2881 CGCAAGTACT ATAAAGACGA GCTGAGACAG ATCCTGGTGA AGCAGAGCGA GTTCCACGCC
 2941 GCTCTGCAGG ATCATAATCT GCTGCTGTCC TGTATCGAAG AGCTGTACCC TAACAATGAG
 3001 GCCCACAGAA GGCTGCTGAG CGCCAGCAGC TTCATCTACT TCCTGATCGA GGACATTCTG
 3061 TTTTATCAGC GCCCACTGAA GTCCCAGAAA GGGCTGATCG ATAACTGCCC CTACGAGTCT
 3121 CACATCTACA AGGATAAGAA AGATGGAAGT CTGCACCATG TGCCTCTGAA GTGTGTCAGC
 3181 AAATCCCATC CACTGTTCCA GGAATTTCGC CTGTGGCAGT TCCTGTCTAA CCTGCGAATC
 3241 TACCAGAGAG AGAGGATGAT GGACGGCAGT CTGAAACTGG ACGTGGATGT CACCCGGGAG
 3301 TTCCTGCCCT CAGAAGAGGA CTACGTGAAG CTGTTTGATT GGCTGAATGA GAGAAAGGAA
 3361 ATCTCTCAGA AATTCCTGCT GGCTTATAAA CCTTTTGGGC TGAAGAAAAA CGAACAGGCA
 3421 AATTACAGAT GGAACTATGT GGAGGACAAG AGCTACCCCT GCAACGAGAC ACGGGCAGAA
 3481 ATCAAGAGCA GACTGTCCAA AGCCGGAGTG CCTGAAGAGT TTCTGACTGA AGAGAAGGAA
 3541 GAGGCCCTGT GGCACATCCT GTATTCTATT AGTGATAAGA AAGAGCTGAC TAAGGCTCTG
 3601 GGCACCTTCG CAGCCAAAAA CTGTCTGAAT GAGTCTTTCG TGGAAGTCTT TGCCAAGATC
 3661 CCCCCTTTTG AGTCAAACTA CGCTGCATAT AGCCTGAAGG CTATTAGGAA ACTGCTGGCA
 3721 CTGATGCGCA TGGGGAAGTA CTGGAATGAA CAGGCCATCG ACAGGCAGAC TCGCGATCGA
 3781 ATCGAGAAAA TTCTGACCGG AGAGTATGAC GAAACAATCC GGAGCAGAGT GAGGGAGAAG
 3841 GCAATGCTGC TGACCGATAT TAGCAGCTTC CGGGGCCTGC CTCTGTGGCT GGCCTGTTAC
 3901 ATCGTGTATG ACCGCCACTC AGAGAGCAAG GAACTGGTCA AATGGGAGAC ACCAGCCGAC
 3961 ATCGATCATT TCCTGTCCAA GTTTAAACAG AACAGCCTGC GGAACCCCAT CGTGGAACAG
 4021 GTCATTACAG AGTCCCTGCG CACTGTGCGA GACATTTGGA AGCAGGAGGG AAAAATCGAT
 4081 GAGATTCACG TGGAACTGGG CCGGGAGATG AAGAACCCTG CAAAAGAGCG CGCCCGAATT
 4141 ACAGCTCAGG TGCAGGAAAA TGAGAACACT AATCTGAGAA TCAAGGCTCT GCTGGCAGAG
 4201 TTCATGAACC CCGAATTTGA GATTGAAAAT GTGCATCCAT ACTCACCCGG CCAGCAGGAA
 4261 ATCCTGCGGA TCTACGAGGA CGGCGTGCTG AGCGGGATCG CTGAGAAGGA TCTGCCTGAC
 4321 GATATCACAG CAATTCTGAA GAAATTCCGA GAAAACGACG TGAAGAAACG GCCAACAACT
 4381 AGCGAAGTCC TGCGGTACAA ACTGTGGCTG GAGCAGCGGT ACAGATCCCC ATATACCGGA
 4441 AGAGTGATCC CCCTGGGCAA GCTGTTCACA CCTGCTTACG AGATCGAACA CGTGATTCCC
 4501 CAGAGCCGGT ATTTTGACGA TTCCATCTCT AACAAAGTGA TCTGCGAAAG TGCCGTCAAT
 4561 AAGCTGAAAG ATAACTGTCT GGGCTATGAG TTCATCAAGA AACATTCCGG GGAGATGGTG
 4621 GAACTGGGGA ATGGAGAGAC AGTGCCCGTG TTCAGCGTGG AAGAGTACGA ACGGTTCGTC
 4681 AAGGAGTCTT ACTTCGGCAA CAGTAAGAAA ATGAAGAAAC TGCTGCTGGA GGACATCCCA
 4741 GATAGCTTCA TTGAGAGACA GCTGAATGAC AGTCGATACA TCTCACGGGT GGTCACATCT
 4801 CTGCTGAGTA ACCTGGTGTG CGAAGAGGGA GAGCAGGATG GCCTGTCCAA GAATGTGATC
 4861 GTCTGTACCG GCGGGATTAC AGACAGGCTG AAGAAAGATT GGGGAGTGCA GGAAGTCTGG
 4921 AACCGCATCA TTCTGCCTCG GTTCCTGAGA CTGAATGAGA TCACCGGACG GACAGACTTT
 4981 ACAAGTACTT CAGTGAACGG CCACCTGCTG CCTGCCCTGC CACTGTACCT GCAGAAGGGC
 5041 TTTAATAAGA AAAGAATTGA CCATAGGCAC CATGCCATGG ATGCTATCGT GATTGCCTGC
 5101 GCTAACCGGA ATATCGTCAA CTACCTGAAC AATTCCTCTG CTAGAAAGAA CAGCGAAATT
 5161 AGCCGATATG ACCTGCAGCG GCTGCTGTGT GAGAAGGTGA AAACCGATGC CAACGGCAAT
 5221 TACAAGTGGA TCCTGAGGAA ACCATGGGAG ACATTCACCC AGGATGTGTA TGCCGCTCTG
 5281 ACAAACATCG TGGTCAGCTT TAAGCAGAAT CTGCGCGTGA TTAATCGAAC CACAAACTAC
 5341 TATCAGCACT ACAACGAGGC AGGGGAAAAG CGCATGATCC CTCAGACCAA AGGCGACAGA
 5401 TGGGCCATTA GAAAGCCAAT GCATAAAGAT ACTGTGTATG GCGAAGTCAA TCTGAGAAAG
 5461 GAGAAAACCC TGCCACTGAG GGACGTGGTC AAGAACCCCA GTATCGTGGT CGATAAATCA
 5521 CTGAAGAACA AGCTGTACGA GCTGCTGAAG AGCCAGTATG ACCTGAAGGC AATCGCCAAA
 5581 TACTTCGAGA CACACCAGGA CGTGTGGGCA GATGTCAACC TGAAGAAAAT TAAGGTGTAC
 5641 TACTTCACTA AGGAGACAAA CGAAAGATTC TTTGCCACTA GGAAGAGCCT GGACCCATCC
 5701 TTTGATCAGA AGAAAATCGA AGAGGAAGTG ACTGATACCG GCGTCCAGAA GATTCTGCTG
 5761 CACCATCTGC AGCAGAACAA TAACGACCCT GATATGGCCT TTTCCCCAGA CGGCATCGAT
 5821 AGGATGAACC AGAATATGAC CATTCTGAAT GACGGGAAGT GGCACCAGCC CATCTACAAA
 5881 GTGCGCACAT ATGAAAAGGC AGATAAATTC GCCGTCGGCG AGTCTGGGAA CAAGGCCAAG
 5941 AAATTTGTGG AAGCAGCCAA AGGCACCAAT CTGTTCTTTG CCGTGTACGA GAGTGTCCAG
 6001 GAGGACGAAG CTATCGGGAA GCAGGTCTGC AAACGGACAT TCGCCACTAT CCCCCTGAAC
 6061 GAAGTGATCA AGAGAAAGAA ACAGGGCCTG CCCGCTGCAC CTGAGGACCT GAACGGGAAT
 6121 CTGCCCAAGT TTGTGCTGAG CCCTAACGAT CTGGTCTACC TGCCCACCGA GGAAGAGAGG
 6181 AATAGTTCAC GCATCATTCA GCCTCTGGAC AGGGAGCGCA TCTATAAGAT GGTGAGCTCC
 6241 TCTGGGAGTC AGTGCTTCTT TATCAAAGTG TTCGTCGCCA ATTCAATTTG GGATAAGAAC
 6301 GAATACAGCA GCCTGAACAA GATGGAGAGG GCTATTACAA ACGAAATGAT CAAGGAGATT
 6361 TGTGTGCCTA TCAAAATTGA CCGCCTGGGC AATGTCAGCC TGATCCAGAT TTAAGAATTC
 6421 ggtaccgagc tcaagcttgc ggccgcctat agtgtcacct aaatcgtaac ccagctttct
 6481 tgtacaaagt ggtcgggtgg catccctgtg acccctcccc agtgcctctc ctggccctgg
 6541 aagttgccac tccagtgccc accagccttg tcctaataaa attaagttgc atcattttgt
 6601 ctgactaggt gtccttctat aatattatgg ggtggagggg ggtggtatgg agcaaggggc
 6661 aagttgggat gacaacctgt agggcctgcg gggtctattg ggaaccaagc tggagtgcag
 6721 tggcacaatc ttggctcact gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc
 6781 agcctcccga gttgttggga ttccaggcat gcatgaccag gctcagctaa tttttgtttt
 6841 tttggtagag acggggtttc accatattgg ccaggctggt ctccaactcc taatctcagg
 6901 tgatctaccc accttggcct cccaaattgc tgggattaca ggcgtgaacc actgctccct
 6961 tccctgtcct tctagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgt
 7021 ctagaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
 7081 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
 7141 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
 7201 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
 7261 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
 7321 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
 7381 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
 7441 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
 7501 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
 7561 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
 7621 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
 7681 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
 7741 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
 7801 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
 7861 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
 7921 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
 7981 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
 8041 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
 8101 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
 8161 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
 8221 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
 8281 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
 8341 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
 8401 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
 8461 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
 8521 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
 8581 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
 8641 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
 8701 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
 8761 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
 8821 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
 8881 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
 8941 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
 9001 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgtt catgaagccc
 9061 cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg
 9121 aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag
 9181 aatgagtatt tggtttagag tttggcaaca tatgcccata tgtaactagc ataacccctt
 9241 ggggcctcta aacgggtctt gaggggtttt ttgctgaaag catgcggagg aaattctcct
 9301 tgaagtttcc ctggtgttca aagtaaagga gtttgcacca gacgcacctc tgttcactgg
 9361 tccggcgtat taaaacacga tacattgtta ttagtacatt tattaagcgc tagattctgt
 9421 gcgttgttga tttacagaca attgttgtac gtattttaat aattcattaa atttataatc
 9481 tttagggtgg tatgttagag cgaaaatcaa atgattttca gcgtctttat atctgaattt
 9541 aaatattaaa tcctcaatag atttgtaaaa taggtttcga ttagtttcaa acaagggttg
 9601 tttttccgaa ccgatggctg gactatctaa tggattttcg ctcaacgcca caaaacttgc
 9661 caaatcttgt agcagcaatc tagctttgtc gatattcgtt tgtgttttgt tttgtaataa
 9721 aggttcgacg tcgttcaaaa tattatgcgc ttttgtattt ctttcatcac tgtcgttagt
 9781 gtacaattga ctcgacgtaa acacgttaaa tagagcttgg acatatttaa catcgggcgt
 9841 gttagcttta ttaggccgat tatcgtcgtc gtcccaaccc tcgtcgttag aagttgcttc
 9901 cgaagacgat tttgccatag ccacacgacg cctattaatt gtgtcggcta acacgtccgc
 9961 gatcaaattt gtagttgagc tttttggaat tgcgatcgca taacttcgta tagcatacat
10021 tatacgaagt tataagctcg gaacgctgcg ctcggtcgtt cggctgcggc gagcggtatc
10081 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
10141 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
10201 tttccatagg ctccgccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
10261 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
10321 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
10381 gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
10441 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
10501 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
10561 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
10621 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
10681 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
10741 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
10801 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
10861 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
10921 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
10981 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
11041 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
11101 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
11161 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
11221 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
11281 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
11341 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
11401 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
11461 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
11521 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
11581 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
11641 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
11701 ttattgtctc atgtccgcgc gtt
//

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