pAAVLINKv1-pCALM1-Cre-3'-ARID2-FLAG

LOCUS       pAAVLINKv1-pCALM1-Cre-3'-ARID2-FLAG            6789 bp DNA     circular SYN 12-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6789)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6789
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     promoter        145..265
                     /label=pCALM1
     CDS             277..1308
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     intron          1309..1349
                     /label=Splicing Donor
     protein_bind    1356..1389
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1390..1481
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1482..3490
                     /codon_start=1
                     /label=ARID2
                     /translation="GLLATR*P*QLCQIRVLHLQL*VREMQLSSLLQQELP*AERRQEL
                     DFQYKRFQPLKHLLLDNHHVLLLLPHSKVIK*FAKRRRKQRKQQVYMFMNVKLKSWRTR
                     PADEEPQTPAMGIQRKMKCMWEVF*MGESTVTQVYLLQTQGKFKVRLISAH*SVMGHHW
                     N*VRMEHLGNRTQNK*TCKISKVI*ENR*LMESVILIKEMVLI*AKTFQIIKLPIM*EM
                     VRYLQWNHKGL*ISLSKILPKVIN*KEFLMDLY*LWVVHL*AVYRRLQMRQHSNLVVLI
                     CLMDL*LQV*IQMCLSNAQV*LSHHILQPLLYRDIKS*QFPTQDQKYPILLPYHLTFGL
                     QMAQQNAKL*RGQQRILIGKQSQEFQIK*ELELLQSVTPTMLAAAQQWLLCQQEQIQAL
                     *LK*Q*KVLFSKSNSIHQHMYRMWSRRTLLCHLHQLYKCRASLTVLSLLHSVDPVSLEI
                     Q*ENLDRTSCVCGSLVKSGFRHPHRFSTMQQLNMEEKMYIQGSVFGKVVSLFSDSGFLL
                     LPTCRISTVQRMPYLQD*NKMNQDKQEVRSLLPSSQL*GAQAQLLEHKRPL*IIPVLHL
                     WL*GEDQETLSFEILQMKKRDQ*LNTSD*QLP*Y*KILVNIQNVVADC*RDMKITYQC*
                     PLVTWKLPPPLPNAFMNLILQFRVRNKKKTQKCC"
     misc_feature    1722..1727
                     /label=SDS
     misc_feature    1725..1730
                     /label=SDS
     misc_feature    1898..1903
                     /label=SDS
     misc_feature    2280..2285
                     /label=SDS
     CDS             3497..3562
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3578..4009
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3722..3727
                     /label=SDS
     repeat_region   4052..4192
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4052..4181
                     /label=AAV2 ITR
     rep_origin      4267..4722
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5004..5108
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5109..5969
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6140..6728
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
      181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
      241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
      301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
      361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
      421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
      481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
      541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
      601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
      661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
      721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
      781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
      841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
      901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
      961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
     1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
     1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
     1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
     1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
     1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
     1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
     1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
     1441 tattggtctt actgacatcc actttgcctt tctctccaca gggacttctg gcaaccaggt
     1501 aaccataaca gttgtgccaa atacgagttt tgcacctgca actgtgagtc agggaaatgc
     1561 aactcagctc attgctccag caggaattac catgagcgga acgcagacag gagttggact
     1621 tccagtacaa acgcttccag ccactcaagc atctcctgct ggacaatcat catgtactac
     1681 tgctactccc ccattcaaag gtgataaaat aatttgccaa aaggaggagg aagcaaagga
     1741 agcaacaggt ttacatgttc atgaacgtaa aattgaagtc atggagaacc cgtcctgccg
     1801 acgaggagcc acaaacacca gcaatgggga tacaaaggaa aatgaaatgc atgtgggaag
     1861 tcttttaaat gggagaaagt acagtgactc aagtctacct ccttcaaact cagggaaaat
     1921 tcaaagtgag actaatcagt gctcactaat cagtaatggg ccatcattgg aattaggtga
     1981 gaatggagca tctgggaaac agaactcaga acaaatagac atgcaagata tcaaaagtga
     2041 tttgagaaaa ccgctagtta atggaatctg tgattttgat aaaggagatg gttctcattt
     2101 aagcaaaaac attccaaatc ataaaacttc caatcatgta ggaaatggtg agatatctcc
     2161 aatggaacca caagggactt tagatatcac tcagcaagat actgccaaag gtgatcaact
     2221 agaaagaatt tctaatggac ctgtattaac tttgggtggt tcatctgtga gcagtataca
     2281 ggaggcttca aatgcggcaa cacagcaatt tagtggtact gatttgctta atggacctct
     2341 agcttcaagt ttgaattcag atgtgcctca gcaacgccca agtgtagttg tctcaccaca
     2401 ttctacaacc tctgttatac agggacatca aatcatagca gttcccgact caggatcaaa
     2461 agtatcccat tctcctgccc tatcatctga cgttcggtct acaaatggca cagcagaatg
     2521 caaaactgta aagaggccag cagaggatac tgatagggaa acagtcgcag gaattccaaa
     2581 taaagtagga gttagaattg ttacaatcag tgaccccaac aatgctggct gcagcgcaac
     2641 aatggttgct gtgccagcag gagcagatcc aagcactgta gctaaagtag caatagaaag
     2701 tgctgttcag caaaagcaac agcatccacc aacatatgta cagaatgtgg tcccgcagaa
     2761 cactcctatg ccaccttcac cagctgtaca agtgcagggc cagcctaaca gttctcagcc
     2821 ttctccattc agtggatcca gtcagcctgg agatccaatg agaaaacctg gacagaactt
     2881 catgtgtctg tggcagtctt gtaaaaagtg gtttcagaca ccctcacagg ttttctacca
     2941 tgcagcaact gaacatggag gaaaagatgt atatccaggg cagtgtcttt gggaaggttg
     3001 tgagcctttt cagcgacagc ggttttcttt tattacccac ttgcaggata agcactgttc
     3061 aaaggatgcc ctacttgcag gattaaaaca agatgaacca ggacaagcag gaagtcagaa
     3121 gtcttctacc aagcagccaa ctgtaggggg cacaagctca actcctagag cacaaaaggc
     3181 cattgtgaat catcccagtg ctgcacttat ggctctgagg agaggatcaa gaaaccttgt
     3241 ctttcgagat tttacagatg aaaaagaggg accaataact aaacacatcc gactaacagc
     3301 tgccttaata ttaaaaaata ttggtaaata ttcagaatgt ggtcgcagat tgttaaagag
     3361 acatgaaaat aacttatcag tgctagccat tagtaacatg gaagcttcct ccacccttgc
     3421 caaatgcctt tatgaactta attttacagt tcagagtaag gaacaagaaa aagactcaga
     3481 aatgctgcag tgtacagact acaaagacca tgacggtgat tataaagatc atgacatcga
     3541 ctacaaggat gacgatgaca agtaagaatt ctgatgactc gagataatca acctctggat
     3601 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     3661 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     3721 tcctccttgt ataaatcctg gttagttctt gccacggcgg aactcatcgc cgcctgcctt
     3781 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gtttatttgt
     3841 gaaatttgtg atgctattgc tttatttgta accatctagc tttatttgtg aaatttgtga
     3901 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     3961 cattcatttt atgtttcagg ttcaggggga gatgtgggag gttttttaaa gatctggaga
     4021 ccggtgtggc acgtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact
     4081 ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg
     4141 ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg
     4201 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac
     4261 catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     4321 tgaccgctac acttgccagc gccttagcgc ccgctccttt cgctttcttc ccttcctttc
     4381 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     4441 gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta
     4501 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     4561 atagtggact cttgttccaa actggaacaa cactcaactc tatctcgggc tattcttttg
     4621 atttataagg gattttgccg atttcggtct attggttaaa aaatgagctg atttaacaaa
     4681 aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta
     4741 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     4801 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     4861 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     4921 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     4981 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     5041 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     5101 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     5161 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     5221 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     5281 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     5341 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     5401 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     5461 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     5521 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     5581 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     5641 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     5701 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     5761 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     5821 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     5881 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     5941 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     6001 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     6061 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     6121 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     6181 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     6241 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     6301 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     6361 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     6421 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     6481 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     6541 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     6601 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     6661 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     6721 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     6781 ctcacatgt
//