| 质粒名称: | pAAV2/1 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 112862 |
| 存储实验室: | James M. Wilson |
|
载体骨架基本信息 |
|
| 载体名称: | -- |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | AAV |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Rep2/Cap1 |
| 物种来源: | AAV |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | Truncated P5 |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | KS primer |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pAAV2/1 was a gift from James M. Wilson (Addgene plasmid # 112862 ; http://n2t.net/addgene:112862 ; RRID:Addgene_112862) |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pAAV2/1 7409 bp ds-DNA circular SYN 07-AUG-2018
DEFINITION AAV packaging plasmid expressing Rep/Cap genes.
ACCESSION .
VERSION .
KEYWORDS pAAV2/1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7409)
TITLE AAV packaging plasmids
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 7409)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 7, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7409
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 111..133
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 125..142
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 126..142
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 152..171
/label=T7
/note="T7 promoter, forward primer"
promoter 152..170
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 195..211
/label=pBluescriptKS
/note="For pBluescript vector"
primer_bind 196..212
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4515..4531)
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4515..4531)
/label=pBluescriptSK
/note="For pBluescript vector"
primer_bind complement(4747..4767)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(4747..4765)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(4786..4802)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4786..4802)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4799..4821)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4810..4826
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4834..4864)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4879..4900
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(5017..5034)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5188..5776)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5268..5287)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5947..6807)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6570..6589
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6808..6912)
/gene="bla"
/label=AmpR promoter
rep_origin complement(6938..7393)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(7075..7096)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 7287..7306
/label=F1ori-R
/note="F1 origin, reverse primer"
ORIGIN
1 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
61 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
121 acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac tcactatagg gcgaattggg
181 taccgggccc cccctcgagg tcgacggtat cgggggagct cgcagggtct ccattttgaa
241 gcgggaggtt tgaacgcgca gccgccatgc cggggtttta cgagattgtg attaaggtcc
301 ccagcgacct tgacgagcat ctgcccggca tttctgacag ctttgtgaac tgggtggccg
361 agaaggaatg ggagttgccg ccagattctg acatggatct gaatctgatt gagcaggcac
421 ccctgaccgt ggccgagaag ctgcagcgcg actttctgac ggaatggcgc cgtgtgagta
481 aggccccgga ggctcttttc tttgtgcaat ttgagaaggg agagagctac ttccacatgc
541 acgtgctcgt ggaaaccacc ggggtgaaat ccatggtttt gggacgtttc ctgagtcaga
601 ttcgcgaaaa actgattcag agaatttacc gcgggatcga gccgactttg ccaaactggt
661 tcgcggtcac aaagaccaga aatggcgccg gaggcgggaa caaggtggtg gatgagtgct
721 acatccccaa ttacttgctc cccaaaaccc agcctgagct ccagtgggcg tggactaata
781 tggaacagta tttaagcgcc tgtttgaatc tcacggagcg taaacggttg gtggcgcagc
841 atctgacgca cgtgtcgcag acgcaggagc agaacaaaga gaatcagaat cccaattctg
901 atgcgccggt gatcagatca aaaacttcag ccaggtacat ggagctggtc gggtggctcg
961 tggacaaggg gattacctcg gagaagcagt ggatccagga ggaccaggcc tcatacatct
1021 ccttcaatgc ggcctccaac tcgcggtccc aaatcaaggc tgccttggac aatgcgggaa
1081 agattatgag cctgactaaa accgcccccg actacctggt gggccagcag cccgtggagg
1141 acatttccag caatcggatt tataaaattt tggaactaaa cgggtacgat ccccaatatg
1201 cggcttccgt ctttctggga tgggccacga aaaagttcgg caagaggaac accatctggc
1261 tgtttgggcc tgcaactacc gggaagacca acatcgcgga ggccatagcc cacactgtgc
1321 ccttctacgg gtgcgtaaac tggaccaatg agaactttcc cttcaacgac tgtgtcgaca
1381 agatggtgat ctggtgggag gaggggaaga tgaccgccaa ggtcgtggag tcggccaaag
1441 ccattctcgg aggaagcaag gtgcgcgtgg accagaaatg caagtcctcg gcccagatag
1501 acccgactcc cgtgatcgtc acctccaaca ccaacatgtg cgccgtgatt gacgggaact
1561 caacgacctt cgaacaccag cagccgttgc aagaccggat gttcaaattt gaactcaccc
1621 gccgtctgga tcatgacttt gggaaggtca ccaagcagga agtcaaagac tttttccggt
1681 gggcaaagga tcacgtggtt gaggtggagc atgaattcta cgtcaaaaag ggtggagcca
1741 agaaaagacc cgcccccagt gacgcagata taagtgagcc caaacgggtg cgcgagtcag
1801 ttgcgcagcc atcgacgtca gacgcggaag cttcgatcaa ctacgcagac aggtaccaaa
1861 acaaatgttc tcgtcacgtg ggcatgaatc tgatgctgtt tccctgcaga caatgcgaga
1921 gaatgaatca gaattcaaat atctgcttca ctcacggaca gaaagactgt ttagagtgct
1981 ttcccgtgtc agaatctcaa cccgtttctg tcgtcaaaaa ggcgtatcag aaactgtgct
2041 acattcatca tatcatggga aaggtgccag acgcttgcac tgcctgcgat ctggtcaatg
2101 tggatttgga tgactgcatc tttgaacaat aaatgattta aatcaggtat ggctgccgat
2161 ggttatcttc cagattggct cgaggacaac ctctctgagg gcattcgcga gtggtgggac
2221 ttgaaacctg gagccccgaa gcccaaagcc aaccagcaaa agcaggacga cggccggggt
2281 ctggtgcttc ctggctacaa gtacctcgga cccttcaacg gactcgacaa gggggagccc
2341 gtcaacgcgg cggacgcagc ggccctcgag cacgacaagg cctacgacca gcagctcaaa
2401 gcgggtgaca atccgtacct gcggtataac cacgccgacg ccgagtttca ggagcgtctg
2461 caagaagata cgtcttttgg gggcaacctc gggcgagcag tcttccaggc caagaagcgg
2521 gttctcgaac ctctcggtct ggttgaggaa ggcgctaaga cggctcctgg aaagaaacgt
2581 ccggtagagc agtcgccaca agagccagac tcctcctcgg gcatcggcaa gacaggccag
2641 cagcccgcta aaaagagact caattttggt cagactggcg actcagagtc agtccccgat
2701 ccacaacctc tcggagaacc tccagcaacc cccgctgctg tgggacctac tacaatggct
2761 tcaggcggtg gcgcaccaat ggcagacaat aacgaaggcg ccgacggagt gggtaatgcc
2821 tcaggaaatt ggcattgcga ttccacatgg ctgggcgaca gagtcatcac caccagcacc
2881 cgcacctggg ccttgcccac ctacaataac cacctctaca agcaaatctc cagtgcttca
2941 acgggggcca gcaacgacaa ccactacttc ggctacagca ccccctgggg gtattttgat
3001 ttcaacagat tccactgcca cttttcacca cgtgactggc agcgactcat caacaacaat
3061 tggggattcc ggcccaagag actcaacttc aaactcttca acatccaagt caaggaggtc
3121 acgacgaatg atggcgtcac aaccatcgct aataacctta ccagcacggt tcaagtcttc
3181 tcggactcgg agtaccagct tccgtacgtc ctcggctctg cgcaccaggg ctgcctccct
3241 ccgttcccgg cggacgtgtt catgattccg caatacggct acctgacgct caacaatggc
3301 agccaagccg tgggacgttc atccttttac tgcctggaat atttcccttc tcagatgctg
3361 agaacgggca acaactttac cttcagctac acctttgagg aagtgccttt ccacagcagc
3421 tacgcgcaca gccagagcct ggaccggctg atgaatcctc tcatcgacca atacctgtat
3481 tacctgaaca gaactcaaaa tcagtccgga agtgcccaaa acaaggactt gctgtttagc
3541 cgtgggtctc cagctggcat gtctgttcag cccaaaaact ggctacctgg accctgttat
3601 cggcagcagc gcgtttctaa aacaaaaaca gacaacaaca acagcaattt tacctggact
3661 ggtgcttcaa aatataacct caatgggcgt gaatccatca tcaaccctgg cactgctatg
3721 gcctcacaca aagacgacga agacaagttc tttcccatga gcggtgtcat gatttttgga
3781 aaagagagcg ccggagcttc aaacactgca ttggacaatg tcatgattac agacgaagag
3841 gaaattaaag ccactaaccc tgtggccacc gaaagatttg ggaccgtggc agtcaatttc
3901 cagagcagca gcacagaccc tgcgaccgga gatgtgcatg ctatgggagc attacctggc
3961 atggtgtggc aagatagaga cgtgtacctg cagggtccca tttgggccaa aattcctcac
4021 acagatggac actttcaccc gtctcctctt atgggcggct ttggactcaa gaacccgcct
4081 cctcagatcc tcatcaaaaa cacgcctgtt cctgcgaatc ctccggcgga gttttcagct
4141 acaaagtttg cttcattcat cacccaatac tccacaggac aagtgagtgt ggaaattgaa
4201 tgggagctgc agaaagaaaa cagcaagcgc tggaatcccg aagtgcagta cacatccaat
4261 tatgcaaaat ctgccaacgt tgattttact gtggacaaca atggacttta tactgagcct
4321 cgccccattg gcacccgtta ccttacccgt cccctgtaat tacgtgttaa tcaataaacc
4381 ggttgattcg tttcagttga actttggtct cctgtccttc ttatcttatc ggttaccatg
4441 gttatagctt acacattaac tgcttggttg cgcttcgcga taaaagactt acgtcatcgg
4501 gttaccccta gggggatcca ctagttctag aggtcctgta ttagaggtca cgtgagtgtt
4561 ttgcgacatt ttgcgacacc atgtggtcac gctgggtatt taagcccgag tgagcacgca
4621 gggtctccat tttgaagcgg gaggtttgaa cgcgcagccg ccaagccgaa ttctgcagat
4681 atccatcaca ctggcggccg ctcgactaga gcggccgcca ccgcggtgga gctccagctt
4741 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
4801 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
4861 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
4921 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
4981 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
5041 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
5101 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
5161 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
5221 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
5281 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
5341 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
5401 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
5461 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
5521 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
5581 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
5641 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
5701 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
5761 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
5821 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
5881 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
5941 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
6001 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
6061 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
6121 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
6181 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
6241 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
6301 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
6361 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
6421 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
6481 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
6541 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
6601 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
6661 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
6721 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
6781 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
6841 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
6901 aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg
6961 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
7021 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
7081 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
7141 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
7201 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
7261 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
7321 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
7381 ctacagggcg cgtcccattc gccattcag
//
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