| 质粒名称: | Cerulean-C1 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 54604 |
| 存储实验室: | Michael Davidson, Dave Piston |
|
载体骨架基本信息 |
|
| 载体名称: | Cerulean-C1 |
| 空载体大小: | 4750 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 卡那霉素，50μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 标签/融合蛋白: | Cerulean（主干上的N端子） |
|
文献引用方法 |
|
| 材料和方法部分: | Cerulean-C1 was a gift from Michael Davidson & Dave Piston (Addgene plasmid # 54604 ; http://n2t.net/addgene:54604 ; RRID:Addgene_54604) |
| 参考文献部分: | An improved cyan fluorescent protein variant useful for FRET. Rizzo MA, Springer GH, Granada B, Piston DW. Nat Biotechnol. 2004 Apr . 22(4):445-9. 10.1038/nbt945 PubMed 14990965 |
买家导航
Cerulean-C1载体质粒基本信息
Cerulean-C1质粒图谱载体图谱和Cerulean-C1载体序列质粒序列多克隆位点信息
Cerulean-C1质粒载体简介
Cerulean-C1质粒序列载体序列
LOCUS Cerulean-C1 4731 bp DNA circular SYN 15-DEC-2025
DEFINITION Localization: C1 Cloning Vector, Excitation: 433, Emission: 475.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4731)
AUTHORS Rizzo MA, Springer GH, Granada B, Piston DW
TITLE An improved cyan fluorescent protein variant useful for FRET.
JOURNAL Nat Biotechnol. 2004 Apr . 22(4):445-9.
PUBMED 14990965
REFERENCE 2 (bases 1 to 4731)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Dec 15, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat
Biotechnol. 2004 Apr . 22(4):445-9."
COMMENT Sequence Label: Cerulean-C1
FEATURES Location/Qualifiers
source 1..4731
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 68..371
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 372..575
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 526..546
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 614..623
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS join(620..622,623..625,626..1336)
/codon_start=1
/product="enhanced monomeric variant of CFP (Rizzo et al.,
2004)"
/label=mCerulean
/note=" mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(665..686)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(926..945)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 1273..1294
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 1337..1402
/label=MCS
/note="multiple cloning site"
polyA_signal 1526..1647
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1563..1582)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 1617..1636
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(1654..2109)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(1791..1812)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2003..2022
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 2136..2240
/gene="bla"
/label=AmpR promoter
promoter 2242..2599
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
enhancer 2242..2433
/label=SV40 enhancer
/note="enhancer for the SV40 early promoter (Herr, 1993)"
primer_bind complement(2265..2285)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 2450..2585
/label=SV40 ori
/note="SV40 origin of replication"
CDS 2634..3428
/codon_start=1
/product="aminoglycoside phosphotransferase"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
CDS 2634..3428
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(2688..2707)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3298..3317
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(3616..3635)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 3660..3707
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4036..4624
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4525..4544
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
541 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
601 agcgctaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
661 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
721 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
781 gcccgtgccc tggcccaccc tcgtgaccac cctgacctgg ggcgtgcagt gcttcgcccg
841 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
901 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
961 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
1021 cggcaacatc ctggggcaca agctggagta caacgccatc agcgacaacg tctatatcac
1081 cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga
1141 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
1201 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
1261 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
1321 ggacgagctg tacaagtccg gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga
1381 cggtaccgcg ggcccgggat ccaccggatc tagataactg atcataatca gccataccac
1441 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
1501 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
1561 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
1621 tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa
1681 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
1741 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
1801 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
1861 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
1921 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
1981 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
2041 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
2101 agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
2161 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
2221 aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
2281 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
2341 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
2401 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
2461 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
2521 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
2581 aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg
2641 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
2701 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
2761 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg
2821 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
2881 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
2941 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
3001 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
3061 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
3121 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg
3181 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
3241 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
3301 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
3361 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
3421 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
3481 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
3541 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
3601 tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac
3661 ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt
3721 tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc
3781 ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc
3841 agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat
3901 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
3961 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
4021 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
4081 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
4141 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
4201 gttaggccac cacttcaaga actctgtatc accgcctaca tacctcgctc tgctaatcct
4261 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
4321 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
4381 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
4441 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
4501 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
4561 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
4621 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
4681 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg c
//
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