| 质粒名称: | HDAC1 Flag |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 13820 |
| 存储实验室: | Eric Verdin |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 + |
| 载体出品公司: | Invitrogen |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5500 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | HDAC1,Histone Deacetylase 1 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 1600 bp |
| 基因突变: | -- |
| GenBank编号 | NM_004964 |
| 启动子 | -- |
| 标签/融合蛋白: | flag（主干上的C终端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | EcoRI(未破坏) |
| 3' 克隆位点: | EcoRI(未破坏) |
| 5' 测序引物 | T7 |
| 3' 测序引物 | BGH Reverse |
|
文献引用方法 |
|
| 材料和方法部分: | HDAC1 Flag was a gift from Eric Verdin (Addgene plasmid # 13820 ; http://n2t.net/addgene:13820 ; RRID:Addgene_13820) |
| 参考文献部分: | Characterization of a human RPD3 ortholog, HDAC3. Emiliani S, Fischle W, Van Lint C, Al-Abed Y, Verdin E. Proc Natl Acad Sci U S A. 1998 Mar 17. 95(6):2795-800. 10.1073/pnas.95.6.2795 PubMed 9501169 |
买家导航
HDAC1Flag载体基本信息
HDAC1Flag载体图谱质粒图谱和多克隆位点信息
HDAC1Flag载体简介
HDAC1Flag载体序列
LOCUS HDAC1 Flag 6869 bp ds-DNA circular SYN 19-MAR-2019
DEFINITION Mammalian expression of human histone deacetylase 1 with flag tag.
ACCESSION .
VERSION .
KEYWORDS HDAC1 Flag
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6869)
AUTHORS Emiliani S, Fischle W, Van Lint C, Al-Abed Y, Verdin E
TITLE Characterization of a human RPD3 ortholog, HDAC3.
JOURNAL Proc Natl Acad Sci U S A. 1998 Mar 17. 95(6):2795-800.
PUBMED 9501169
REFERENCE 2 (bases 1 to 6869)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Mar 19, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6869
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 74..453
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 454..657
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 608..628
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 702..721
/label=T7
/note="T7 promoter, forward primer"
promoter 702..720
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 2241..2264
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
primer_bind complement(2299..2316)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 2305..2529
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 2575..3003
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2662..2681)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 2872..2893
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(3012..3032)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 3017..3346
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3197..3332
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3259..3278
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 3413..4207
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3467..3486)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 4077..4096
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 4381..4502
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4418..4437)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 4472..4491
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(4551..4567)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4551..4567)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4564..4586)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4575..4591
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4599..4629)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4644..4665
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4782..4799)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4953..5541)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5033..5052)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5712..6572)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6335..6354
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6573..6677)
/gene="bla"
/label=AmpR promoter
primer_bind complement(6752..6771)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
61 agatatacgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
121 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
181 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
241 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
301 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
361 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
421 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
481 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
541 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
601 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc
661 tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg
721 gagacccaag ctggctagcg tttaaactta agcttggtac cgagctcgga tccgaattca
781 cgatggcgca gacgcagggc acccggagga aagtctgtta ctactacgac ggggatgttg
841 gaaattacta ttatggacaa ggccacccaa tgaagcctca ccgaatccgc atgactcata
901 atttgctgct caactatggt ctctaccgaa aaatggaaat ctatcgccct cacaaagcca
961 atgctgagga gatgaccaag taccacagcg atgactacat taaattcttg cgctccatcc
1021 gtccagataa catgtcggag tacagcaagc agatgcagag attcaacgtt ggtgaggact
1081 gtccagtatt cgatggcctg tttgagttct gtcagttgtc tactggtggt tctgtggcaa
1141 gtgctgtgaa acttaataag cagcagacgg acatcgctgt gaattgggct gggggcctgc
1201 accatgcaaa gaagtccgag gcatctggct tctgttacgt caatgatatc gtcttggcca
1261 tcctggaact gctaaagtat caccagaggg tgctgtacat tgacattgat attcaccatg
1321 gtgacggcgt ggaagaggcc ttctacacca cggaccgggt catgactgtg tcctttcata
1381 agtatggaga gtacttccca ggaactgggg acctacggga tatcggggct ggcaaaggca
1441 agtattatgc tgttaactac ccgctccgag acgggattga tgacgagtcc tatgaggcca
1501 ttttcaagcc ggtcatgtcc aaagtaatgg agatgttcca gcctagtgcg gtggtcttac
1561 agtgtggctc agactcccta tctggggatc ggttaggttg cttcaatcta actatcaaag
1621 gacacgccaa gtgtgtggaa tttgtcaaga gctttaacct gcctatgctg atgctgggag
1681 gcggtggtta caccattcgt aacgttgccc ggtgctggac atatgagaca gctgtggccc
1741 tggatacgga gatccctaat gagcttccat acaatgacta ctttgaatac tttggaccag
1801 atttcaagct ccacatcagt ccttccaata tgactaacca gaacacgaat gagtacctgg
1861 agaagatcaa acagcgactg tttgagaacc ttagaatgct gccgcacgca cctggggtcc
1921 aaatgcaggc gattcctgag gacgccatcc ctgaggagag tggcgatgag gacgaagacg
1981 accctgacaa gcgcatctcg atctgctcct ctgacaaacg aattgcctgt gaggaagagt
2041 tctccgattc tgaagaggag ggagaggggg gccgcaagaa ctcttccaac ttcaaaaaag
2101 ccaagagagt caaaacagag gatgaaaaag agaaagaccc agaggagaag aaagaagtca
2161 ccgaagagga gaaaaccaag gaggagaagc cagaagccaa aggggtcaag gaggaggtca
2221 agttggccga attcggagga gactacaagg acgacgatga caagtaatct agagggcccg
2281 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
2341 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
2401 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
2461 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
2521 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
2581 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
2641 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
2701 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
2761 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
2821 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
2881 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
2941 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
3001 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
3061 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
3121 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
3181 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
3241 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
3301 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
3361 ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga
3421 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
3481 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
3541 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
3601 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
3661 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
3721 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
3781 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
3841 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
3901 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
3961 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
4021 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
4081 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
4141 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
4201 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
4261 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
4321 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
4381 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
4441 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
4501 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
4561 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
4621 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
4681 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
4741 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
4801 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
4861 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
4921 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
4981 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
5041 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
5101 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
5161 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
5221 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
5281 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
5341 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
5401 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
5461 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
5521 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
5581 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
5641 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
5701 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
5761 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
5821 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
5881 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
5941 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
6001 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
6061 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
6121 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
6181 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
6241 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
6301 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
6361 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
6421 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
6481 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
6541 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
6601 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
6661 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga
6721 gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
6781 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt
6841 aagctacaac aaggcaaggc ttgaccgac
//
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