| 质粒名称: | pLH-sgRNA1 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 75388 |
| 存储实验室: | Thoru Pederson |
|
载体骨架基本信息 |
|
| 载体名称: | pLKO.1 |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 8000 bp |
| 完整质粒大小: | 8267 bp |
| 载体类型: | 哺乳动物表达，慢病毒，CRISPR |
| 筛选标记: | 潮霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | sgRNA1 |
| 物种来源: | 合成 |
| 插入大小: | 76 bp |
| 启动子 | U6 |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | Sbf I(未破坏) |
| 3' 克隆位点: | EcoR I(未破坏) |
| 5' 测序引物 | cccttcaccgagggcctatttccc |
| 3' 测序引物 | CTATTCTTTCCCCTGCACTGTACCC |
|
文献引用方法 |
|
| 材料和方法部分: | pLH-sgRNA1 was a gift from Thoru Pederson (Addgene plasmid # 75388 ; http://n2t.net/addgene:75388 ; RRID:Addgene_75388) |
| 参考文献部分: | Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow. Ma H, Tu LC, Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T. Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526. 10.1038/nbt.3526 PubMed 27088723 |
买家导航
pLHsgRNA1载体基本信息
pLHsgRNA1载体图谱质粒图谱和多克隆位点信息
pLHsgRNA1载体简介
pLHsgRNA1载体序列
LOCUS pLH-sgRNA1 8267 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION sgRNA1.
ACCESSION .
VERSION .
KEYWORDS pLH-sgRNA1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8267)
AUTHORS Ma H, Tu LC, Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T
TITLE Multiplexed labeling of genomic loci with dCas9 and engineered
sgRNAs using CRISPRainbow.
JOURNAL Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
PUBMED 27088723
REFERENCE 2 (bases 1 to 8267)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8267
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 51..72
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 87..117
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 125..141
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 130..152
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 149..165
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 149..165
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 184..204
/label=T3
/note="T3 promoter, forward primer"
promoter 186..204
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 232..458
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 459..639
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 686..811
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1304..1537
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
promoter 2064..2304
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
primer_bind 2064..2084
/label=hU6-F
/note="Human U6 promoter, forward primer"
primer_bind 2235..2254
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
CDS 2653..2958
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind 2837..2856
/label=ccdB-fwd
/note="ccdB gene, forward primer"
misc_RNA 2979..3054
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
misc_feature 3103..3220
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 3269..3779
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
primer_bind 3698..3716
/label=hPGK-F
/note="Human PGK promoter, forward primer"
CDS 3869..4894
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
E"
primer_bind complement(4918..4934)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4919..4935)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 5017..5250
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 5322..5443
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5359..5378)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5413..5432
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 5483..5618
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 5545..5564
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(5638..5657)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(5639..5657)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(5667..5684)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(5667..5683)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5676..5698)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 5825..6280
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5912..5931)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6122..6143
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 6306..6410
/gene="bla"
/label=AmpR promoter
CDS 6411..7271
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6629..6648)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7442..8030
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 7931..7950
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 8184..8201
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
61 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
121 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
181 cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt
241 atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag
301 gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat
361 taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca
421 gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga
481 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
541 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
601 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
661 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
721 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
781 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
841 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
901 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
961 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
1021 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
1081 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
1141 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
1201 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
1261 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
1321 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
1381 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
1441 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
1501 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
1561 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
1621 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
1681 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
1741 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
1801 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
1861 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
1921 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
1981 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgatca cgagactagc
2041 ctcgagcggc cgcccccttc accgagggcc tatttcccat gattccttca tatttgcata
2101 tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat
2161 tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat
2221 tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg
2281 ctttatatat cttgtggaaa ggacgaaaca ccggtgtctt cgaggcttac taaaagccag
2341 ataacagtat gcgtatttgc gcgctgattt ttgcggtata agaatatata ctgatatgta
2401 tacccgaagt atgtcaaaaa gaggtgtgct atgaagcagc gtattacagt gacagttgac
2461 agcgacagct atcagttgct caaggcatat atgatgtcaa tatctccggt ctggtaagca
2521 caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg
2581 aagggatggc tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct gacgagaaca
2641 gggactggtg aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg
2701 tttgtggatg tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg
2761 gccagtgcac gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc
2821 ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc
2881 ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg
2941 atgttctggg gaatataaac cctgcaggac gaagacccgt ttgagagcta gaaatagcaa
3001 gttcaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt
3061 tgaattctcg acctcgagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
3121 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
3181 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
3241 cagagatcca ctttggccgc ggctcgaggg ggttggggtt gcgccttttc caaggcagcc
3301 ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg
3361 accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta
3421 cccttgtggg ccccccggcg acgcttcctg ctccgcccct aagtcgggaa ggttccttgc
3481 ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg tctcactagt accctcgcag
3541 acggacagcg ccagggagca atggcagcgc gccgaccgcg atgggctgtg gccaatagcg
3601 gctgctcagc agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt
3661 gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc
3721 cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat caccgacctc tctccccagg
3781 gggatccacc ggatcaagct tgccagctgg ggcgccctct ggtaaggttg ggaagccctg
3841 cagatctgat cccggggggc aatgagatat gaaaaagcct gaactcaccg cgacgtctgt
3901 cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg
3961 cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa
4021 tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc
4081 gctcccgatt ccggaagtgc ttgacattgg ggaatttagc gagagcctga cctattgcat
4141 ctcccgccgt gcacagggtg tcacgttgca agatctgcct gaaaccgaac tgcccgctgt
4201 tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag
4261 cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat
4321 atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag
4381 tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt
4441 ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat
4501 aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa
4561 catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg
4621 gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct
4681 tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg
4741 tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg
4801 cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg
4861 acgccccagc actcgtccga gggcaaagga atagagtaga tgccgaccgg gatctatcga
4921 taccgtcgac ctcgaggggg ggcccggtac ctttaagacc aatgacttac aaggcagctg
4981 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
5041 gaagaaaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
5101 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
5161 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
5221 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
5281 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
5341 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
5401 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
5461 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
5521 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
5581 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
5641 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
5701 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
5761 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
5821 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
5881 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
5941 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
6001 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
6061 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
6121 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
6181 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
6241 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
6301 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
6361 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
6421 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
6481 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
6541 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
6601 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
6661 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
6721 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
6781 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
6841 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
6901 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
6961 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
7021 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
7081 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
7141 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
7201 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
7261 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
7321 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
7381 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
7441 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
7501 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
7561 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
7621 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
7681 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
7741 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
7801 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
7861 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
7921 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
7981 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
8041 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
8101 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
8161 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
8221 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagc
//
