pLVX-TRE3G-ZsGreen1

价格:1800元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pLVX-TRE3G-ZsGreen1, pLVX TRE3G ZsGreen1
质粒类型: 哺乳动物细胞慢病毒表达载体
高拷贝/低拷贝: 高拷贝
启动子: TRE3GV
克隆方法: 多克隆位点,限制性内切酶
载体大小: 9100 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄
筛选标记: 嘌呤霉素
备注: 包含有Tet-On 3G反式激活蛋白,多西环素(doxycycline)诱导基因表达的慢病毒载体,含有ZsGreen1绿色荧光蛋白标签。
产品目录号: 631350, 631353, 631356
稳定性: /
组成型: 诱导型
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLVX-TRE3G-ZsGreen1载体图谱



pLVX-TRE3G-ZsGreen1多克隆位点

pLVX-TRE3G-ZsGreen1载体特征

载体简介

载体序列

LOCUS       pLVX-TRE3G-ZsGreen1   9100 bp    DNA   circular             9-MAY-2012
COMMENT     Created by Clontech Laboratories Inc.
COMMENT     631361, 631350, 631353, 631356
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     misc_feature    1..635
                     /label=5'\LTR
     misc_feature    685..822
                     /label=packaging\signal
     misc_feature    1303..1536
                     /label=RRE
     misc_feature    2028..2151
                     /label=cPPT/CTS
     misc_feature    3881..4384
                     /label=Ppgk
     misc_feature    4405..5004
                     /label=Puro(R)
     misc_feature    5022..5613
                     /label=WPRE
     3'clip          5817..6453
                     /label=3'\LTR
     misc_feature    complement(6923..7593)
                     /label=pUC\ori
     misc_feature    3268..3856
                     /label=IRES
     misc_feature    3857..3880
                     /label=MCS
     misc_feature    2209..2560
                     /label=pTRE3GV
     misc_feature    2564..3259
                     /label=ZsGreen1
     misc_feature    complement(8599..8703)
                     /label=pAmp(R)
     misc_feature    complement(7738..8598)
                     /label=Amp(R)
BASE COUNT     2257 a      2347 c      2391 g      2105 t 
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgagtt tactccctat 
     2221 cagtgataga gaacgtatga agagtttact ccctatcagt gatagagaac gtatgcagac 
     2281 tttactccct atcagtgata gagaacgtat aaggagttta ctccctatca gtgatagaga 
     2341 acgtatgacc agtttactcc ctatcagtga tagagaacgt atctacagtt tactccctat 
     2401 cagtgataga gaacgtatat ccagtttact ccctatcagt gatagagaac gtatgtcgag 
     2461 gtaggcgtgt acggtgggcg cctataaaag cagagctcgt ttagtgaacc gtcagatcgc 
     2521 ctggagcaat tccacaacac ttttgtctta tacttggatc accatggctc agtcaaagca 
     2581 cggcctgacc aaggagatga ccatgaagta ccgcatggag ggctgcgtgg acggccacaa 
     2641 gttcgtgatc accggcgagg gcatcggcta ccccttcaag ggcaagcagg ccatcaacct 
     2701 gtgcgtggtg gagggcggcc ccttgccctt cgccgaggac atcttgtccg ccgccttcat 
     2761 gtacggcaac cgcgtgttca ccgagtaccc ccaggacatc gtcgactact tcaagaactc 
     2821 ctgccccgcc ggctacacct gggaccgctc cttcctgttc gaggacggcg ccgtgtgcat 
     2881 ctgcaacgcc gacatcaccg tgagcgtgga ggagaactgc atgtaccacg agtccaagtt 
     2941 ctacggcgtg aacttccccg ccgacggccc cgtgatgaag aagatgaccg acaactggga 
     3001 gccctcctgc gagaagatca tccccgtgcc caagcagggc atcttgaagg gcgacgtgag 
     3061 catgtacctg ctgctgaagg acggtggccg cttgcgctgc cagttcgaca ccgtgtacaa 
     3121 ggccaagtcc gtgccccgca agatgcccga ctggcacttc atccagcaca agctgacccg 
     3181 cgaggaccgc agcgacgcca agaaccagaa gtggcacctg accgagcacg ccatcgcctc 
     3241 cggatctgca ttgccctgag gccgccccgg ccggcccctc tccctccccc cccccctaac 
     3301 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc 
     3361 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg 
     3421 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg 
     3481 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc 
     3541 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa 
     3601 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa 
     3661 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta 
     3721 ccccattgta tgggatctga tctggggcct cggtacacat gctttacatg tgtttagtcg 
     3781 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca 
     3841 cgatgataat atggcccccg ggacgcgtca tatggaattc taccgggtag gggaggcgct 
     3901 tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac 
     3961 aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt 
     4021 ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc 
     4081 cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt 
     4141 gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata 
     4201 gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg 
     4261 gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt 
     4321 ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct 
     4381 ttcgacctgc agcccaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc 
     4441 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc 
     4501 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc 
     4561 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg 
     4621 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc 
     4681 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc 
     4741 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc 
     4801 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag 
     4861 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag 
     4921 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc 
     4981 atgacccgca agcccggtgc ctgacggggc gcgtctggaa caatcaacct ctggattaca 
     5041 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat 
     5101 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct 
     5161 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac 
     5221 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca 
     5281 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca 
     5341 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg 
     5401 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga 
     5461 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt 
     5521 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga 
     5581 gtcggatctc cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga 
     5641 aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct 
     5701 agaagcacaa gaggaggagg aggtgggttt ttccagtcac acctcaggta cctttaagac 
     5761 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag aggggactgg 
     5821 aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac 
     5881 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga 
     5941 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata 
     6001 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga 
     6061 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc 
     6121 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg 
     6181 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct 
     6241 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc 
     6301 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga 
     6361 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga 
     6421 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag 
     6481 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca ttgctagcgt 
     6541 tttaccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg 
     6601 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 
     6661 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 
     6721 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 
     6781 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 
     6841 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 
     6901 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 
     6961 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 
     7021 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 
     7081 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 
     7141 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 
     7201 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 
     7261 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 
     7321 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 
     7381 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg 
     7441 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 
     7501 aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 
     7561 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 
     7621 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 
     7681 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 
     7741 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 
     7801 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 
     7861 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 
     7921 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 
     7981 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 
     8041 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 
     8101 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 
     8161 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 
     8221 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 
     8281 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 
     8341 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 
     8401 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 
     8461 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 
     8521 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 
     8581 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 
     8641 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 
     8701 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc aacttgttta 
     8761 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 
     8821 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 
     8881 ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta 
     8941 ttaccactgc caattaccta gtggtttcat ttactctaaa cctgtgattc ctctgaatta 
     9001 ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt agtttttaaa 
     9061 gaaattgtat ttgttaaata tgtactacaa acttagtagt 
//

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